Using 1–8 years bamboo as materials,the content of different chemical constituent was tested,and the reactive oxygen species(ROS)free radicals produced from laccase treated bamboo were detected by electron spin-reson...Using 1–8 years bamboo as materials,the content of different chemical constituent was tested,and the reactive oxygen species(ROS)free radicals produced from laccase treated bamboo were detected by electron spin-resonance(ESR)spectroscope.The wet-process particleboard was made from laccase-treated bamboo by hot pressing and board mechanical properties including internal bond strength(IB),modulus of rupture(MOR)and thickness swelling(TS)after 2-hours water absorption were tested under different conditions.Results showed that laccase mainly catalyze the bamboo components and improved the bonding strength of laccase-treated boards.By ESR measurement on each single component such as milled bamboo lignin,xylan and pore cotton treated with laccase,it was proved that laccase helped the degradation of bamboo lignin to produce ROS free radicals and could not catalyze the oxidation of cellulose and hemicelluloses.A logarithmic function relationship was found between board mechanical properties and ROS free radical level.It is optimal to using 5-year-old bamboo for high efficient utilization.The laccase treatment improves the activity of bamboo particles participating in self-adhesion reaction.展开更多
基金The authors thank Zhejiang Provincial Science and Technology Department Project(2019F1065-4)&Zhejiang Provincial Natural Science Foundation(LY19C160004)&Public Welfare Technology Research Agricultural Project of Zhejiang Province(2013C32104)for their financial contributions.
文摘Using 1–8 years bamboo as materials,the content of different chemical constituent was tested,and the reactive oxygen species(ROS)free radicals produced from laccase treated bamboo were detected by electron spin-resonance(ESR)spectroscope.The wet-process particleboard was made from laccase-treated bamboo by hot pressing and board mechanical properties including internal bond strength(IB),modulus of rupture(MOR)and thickness swelling(TS)after 2-hours water absorption were tested under different conditions.Results showed that laccase mainly catalyze the bamboo components and improved the bonding strength of laccase-treated boards.By ESR measurement on each single component such as milled bamboo lignin,xylan and pore cotton treated with laccase,it was proved that laccase helped the degradation of bamboo lignin to produce ROS free radicals and could not catalyze the oxidation of cellulose and hemicelluloses.A logarithmic function relationship was found between board mechanical properties and ROS free radical level.It is optimal to using 5-year-old bamboo for high efficient utilization.The laccase treatment improves the activity of bamboo particles participating in self-adhesion reaction.
