Banana streak virus (BSV) and Sugarcane bacilliform virus (SCBV) are two badnaviruses commonly found in all banana growing areas of the world. It is a threat to the production and improvement of Musa germplasm. In Bur...Banana streak virus (BSV) and Sugarcane bacilliform virus (SCBV) are two badnaviruses commonly found in all banana growing areas of the world. It is a threat to the production and improvement of Musa germplasm. In Burkina Faso, the presence of badnaviruses was reported in banana producing regions. The objective of this study was to determine the prevalence of BSV and SCBV in banana production areas of Burkina Faso. A survey followed by a symptomatologic study was conducted in banana plantations in 27 localities of the nine main banana producing regions from July to October 2018 and September to December 2020. In all, 251 leaf samples were collected and analysed for BSV and SCBV infection by Indirect Antigen Coated Plate Assay-ELISA followed by amplification of the RT/RNase H region using Polymerase chain reaction with Badna FP/RP and SCBV F/R primers, respectively. A variety of symptoms were observed on almost all plant organs which were revealed due to BSV by symptomatologic study. The results of serological and molecular diagnosis revealed a high overall prevalence of BSV in 80.48% of the samples tested. BSV was distributed in seven survey regions out of nine with prevalence ranging from 10% to 100% in North, Centre, Centre West, Hauts Bassins, Cascades, Centre East and Boucle of Mouhoun regions. Very low prevalence was recorded for SCBV in Cascades and East Centre region with 4.35 and 12.5%, respectively. Species detection using specific primers to each species revealed three main species: Banana streak Obino l’ewaï virus (BSOLV), Goldfinger virus (BSGFV) and Imové virus (BSIMV) in the samples tested, respectively in the proportions of 23%, 8% and 0.8%. Co-infection between BSV species was also detected.展开更多
香蕉品种‘Agbagaba’和‘Orishele’的胚性细胞悬浮系(ECS)在液体培养基中分别预培养1和2周后,将其接种在RD1和M3培养基上,于光照或黑暗条件下进行体胚的再生。从沉积细胞体积(SCV) 为1 mL(1 mL SCV)的ECS获得的再生体胚数量因预培养...香蕉品种‘Agbagaba’和‘Orishele’的胚性细胞悬浮系(ECS)在液体培养基中分别预培养1和2周后,将其接种在RD1和M3培养基上,于光照或黑暗条件下进行体胚的再生。从沉积细胞体积(SCV) 为1 mL(1 mL SCV)的ECS获得的再生体胚数量因预培养时间、再生培养基的种类及培养条件的不同而异。植株的再生率及从1 mL SCV的ECS获得的再生植株数量也受上述体胚再生条件的间接影响。展开更多
植物光系统Ⅰ反应中心亚基Ⅴ(photosystemⅠreaction center subunitⅤ,简称PSAG或PSⅠ-G)是光合系统Ⅰ的主要组件,具有维持PSⅠ复合体稳定性的重要作用,并与抗盐密切相关。本研究以福州宦溪野生蕉(Musa spp.AB group)叶片为材料,采用...植物光系统Ⅰ反应中心亚基Ⅴ(photosystemⅠreaction center subunitⅤ,简称PSAG或PSⅠ-G)是光合系统Ⅰ的主要组件,具有维持PSⅠ复合体稳定性的重要作用,并与抗盐密切相关。本研究以福州宦溪野生蕉(Musa spp.AB group)叶片为材料,采用同源克隆的方法 ,首次分离到PSAG基因的2个成员:PSAG1、PSAG2(GenBank登录号分别为JX317082、JX317083),分别为800、827 bp,分别编码150、160个氨基酸;PSAG1、PSAG2的基因组序列分析表明2个成员均没有内含子。生物信息学分析表明:PSAG1、PSAG2具有PSⅠ的Ⅹ亚基超家族(photosystemⅠreaction center subunitⅩpsaK)保守结构域,是不具有信号肽的跨膜蛋白,具有亲水性;PSAG1、PSAG2均有4个位点发生磷酸化。宦溪野生蕉PSAG在进化过程中形成了特殊的结构特征,即PSAG1和PSAG2没有内含子,并且在不同物种间保守区具有高度的一致性,为保持PSAG功能的稳定性提供了重要保证。展开更多
The center of diversity of the genus Musa (Musaceae) is in Southeast Asia, a region not studied in detail and where new species and varieties continue to be reported. A new wild banana species, M. chunii Hakkinen fr...The center of diversity of the genus Musa (Musaceae) is in Southeast Asia, a region not studied in detail and where new species and varieties continue to be reported. A new wild banana species, M. chunii Hakkinen from Yunnan, China is described and illustrated based on observed morphological characteristics in the field. This extremely rare new species was only found in Tongbiguan Nature Reserve, Dehong District, West Yunnan. A key to M. chunii and related taxa is provided. In addition, critical notes regarding M. rubra Kurz identity are given.展开更多
Objective:To study the jelly formulation produced by Musa acuminata Colla(AAA Group) peels and evaluate its antioxidant properties which are related to the product quality.Methods:The formulations of peel jelly were e...Objective:To study the jelly formulation produced by Musa acuminata Colla(AAA Group) peels and evaluate its antioxidant properties which are related to the product quality.Methods:The formulations of peel jelly were established under two-level full factorial designs within two blocks and one center point.Regarding response optimizer,the amount of sugar and citric acid was obtained;hence,the peel jellies were produced.The evaluation of antioxidant properties was conducted by using total phenolic content(TPC)assay and 1,1 diphenyl-2-picrylhydrazyl(DPPH) free radical assay.Results:The TPC of peel powder varied from 91.8 to 602.26 mg gallic acid equivalents/100 g dry weight,and 5%-7% peel jellies had phenolic content ranging from 29.38 to 48.31 mg gallic acid equivalents/100 g dry weight.The results of DPPH test indicated that at 10 mg/mL,the peel powder showed 89% DPPH inhibition,while 7% peel jelly prominently exhibited 84% DPPH inhibition.The correlation between DPPH IC50 value and TPC of peel powder as well as peel jelly was quite reasonably high with correlation coefficient ranging from 0.843 7 to 0.995.Conclusions:TPC can be used as an indicator in assessing the antioxidant activity of fruits and vegetables.The present investigation reveals that TPC is mainly responsible for DPPH free radical scavenging capacity.展开更多
Since the initial description, the name Musa aurantiaca Baker (1893) has been unclear to most botanists. The aim of this study is to settle its true identity and to update the description. The plant is distributed in ...Since the initial description, the name Musa aurantiaca Baker (1893) has been unclear to most botanists. The aim of this study is to settle its true identity and to update the description. The plant is distributed in the regions of Upper Assam and Arunachal Pradesh, India, Northern Myanmar and Tibet, China where it occurs commonly but it is not mentioned in Chinese literature at all. In this paper, the authors also review the description and the literature history of M. aurantiaca from 1893 to the present. Musa aurantiaca Baker is typified here.展开更多
Objective:Chilling tolerance of salicylic acid(SA) in banana seedlings(Musa acuminata cv.,Williams 8818) was investigated by changes in ultrastructure in this study.Methods:Light and electron microscope observation.Re...Objective:Chilling tolerance of salicylic acid(SA) in banana seedlings(Musa acuminata cv.,Williams 8818) was investigated by changes in ultrastructure in this study.Methods:Light and electron microscope observation.Results:Pretreatment with 0.5 mmol/L SA under normal growth conditions(30/22 °C) by foliar spray and root irrigation resulted in many changes in ultrastructure of banana cells,such as cells separation from palisade parenchymas,the appearance of crevices in cell walls,the swelling of grana and stromal thylakoids,and a reduction in the number of starch granules.These results implied that SA treatment at 30/22 °C could be a type of stress.During 3 d of exposure to 7 °C chilling stress under low light,however,cell ultrastructure of SA-pretreated banana seedlings showed less deterioration than those of control seedlings(distilled water-pretreated).Conclusion:SA could provide some protection for cell structure of chilling-stressed banana seedling.展开更多
Since the initial description, the name Musa dasycarpa Kurz (1867) has been unclear to most botanists. It has usually been synonymized with M. velutina H. Wendl. & Drude (1875). However, although the original diag...Since the initial description, the name Musa dasycarpa Kurz (1867) has been unclear to most botanists. It has usually been synonymized with M. velutina H. Wendl. & Drude (1875). However, although the original diagnosis was very short, 'fruits hairy', it is adequate. Thus, according to Vienna Codes, M. dasycarpa Kurz has priority over M. velutina H. Wendl. & Drude. The aim of this study is to settle the true identity and to update the description of M. dasycarpa Kurz. For that purpose the names M. dasycarpa and M. velutina are typified. In addi-tion, critical notes regarding M. assamica Bull. are given; it is neotypified here, and considered as conspecific with M. sanguinea Hook. f.展开更多
Organic agriculture promotes disease suppression through healthy soils by increasing biological activity and diversity through the application of organic fertilizers and increasing organic inputs. Fusarium wilt of ban...Organic agriculture promotes disease suppression through healthy soils by increasing biological activity and diversity through the application of organic fertilizers and increasing organic inputs. Fusarium wilt of bananas (Fusarium oxysporum f. sp. Fcubense) (Foc), also known as Panama disease, has been a devastating disease throughout the world. So far, no fungicides or cultural measures have been found that control Foc sufficiently. The aim of this research was to assess whether organic-based farming systems were more resilient than inorganic farming systems to soil borne diseases, in particular Fusarium wilts. A survey was conducted comparing five organic and five conventional banana plantations at paired sites in north Queensland, Australia. Soil samples were collected and analysed for chemical, physical and biological soil health indicators. Disease development of F. oxysporum f. sp. lycopersici in tomatoes and Foc in bananas were studied in pot trials to pursue clues for identifying Fusarium suppressive soil traits. Organic soils from the survey showed higher microbial activity and lower disease symptom expression (both with tomatoes and bananas) than conventional soils. In the survey, nematode diversity and soil sulphate content were recurring indicators in all experiments showing close correlations to pathogen growth, disease expression and plant health. Organic soils were lower in plant-parasitic nematodes and sulphate sulphur levels and higher in nematode diversity, labile soil C and microbial indicators. Soil conduciveness or suppression of Foc appeared to be largely governed by competition for carbon. Measurement of soil microbial enzyme activity, nematode community structure and diversity and possibly sulphate sulphur seem to provide a relatively reliable indicator for general disease suppression. Differences between organic and conventional agriculture cannot be related to single management practices, but may be linked to synergies among system components.展开更多
Objective:To investigate the potential of Musa x paradisiaca(M.x paradisiaca)flower extracts in promoting milk production of lactating rats and its effects on growth of the suckling pups. Methods:Galactagogue activity...Objective:To investigate the potential of Musa x paradisiaca(M.x paradisiaca)flower extracts in promoting milk production of lactating rats and its effects on growth of the suckling pups. Methods:Galactagogue activity was evaluated in terms of quantity of milk produced from the rats treated with petroleum ether,ethanol or water extracts of the flower.Lactating rats(n=5) of Spraque Dawley with six pups each were administered with the extracts in the amount of 500 mg/kg body weight,while the control rats were given an equivalent amount of distilled water.The rats were daily administered via oral feeding starting from Day 5 until Day 14 and the performance of milk production was measured along the experimental period by weight-suckle-weight method. Results were statistically analyzed using SPSS by means of ANOVA at 0.05 and was expressed as their mean±standard deviation.The rates of pups’ growth were measured as the weight gain along the experimental period.Results:The rats treated with aqueous extract produced higher milk than control and ethanol groups.Aqueous extract was identified to increase milk production by 25%,while petroleum ether extract by 18%.The mean of yields produced by the rats during suckling period for aqueous,petroleum ether,ethanol and control were 4.62±2.45,4.37±1.93,3.65±1.89 and 3.69±1.79,respectively.Growth rates of pups for the rats treated with control,aqueous, ethanol extract and petroleum ether were(1.85±0.49),(1.78±0.56),(1.65±0.46)and(1.56±0.42) g/pup, respectively.Conclusions:The present study reveals the potential of M.x paradisiaca flower to enhance milk production of nursing mothers which could be exploited for commercialization of the isolated extract.展开更多
The genus Musa is one of three genera in the family Musaceae, which includes bananas and plantains, which are monocotyledonous plants. Bananas have valuable nutritional content of vitamin C, B6, minerals, and dietary ...The genus Musa is one of three genera in the family Musaceae, which includes bananas and plantains, which are monocotyledonous plants. Bananas have valuable nutritional content of vitamin C, B6, minerals, and dietary fiber and are a rich food energy source, given that carbohydrates account for 22%–32% of fruit weight. Molecular markers are valuable for crop improvement and population genetics studies. The availability of whole-genome sequence and in silico approaches has revolutionized bulk marker discovery. We describe an online web genomic resource, BanSatDB(http://webtom.cabgrid.res.in/bansatdb/) having the highest number(>341,000) of putative STR markers from Musa genera so far, represented by three species: M. acuminata(110,000), M. balbisiana(107,000), and M. itinerans(124,000)from 11 chromosomes of each species. BanSatDB has also been populated with 580 validated STR markers from the published literature. It is based on a three-tier architecture using MySQL, PHP and Apache. The markers can be retrieved by use of multiple search parameters including chromosome number(s), microsatellite types(simple or compound),repeat nucleotides(1–6), copy number, microsatellite length, pattern of repeat motif, and chromosome location. These markers can be used for Distinctness, Uniformity and Stability(DUS) tests of variety identification and for marker assisted selection(MAS) in variety improvement and management. These STRs have also proved to be helpful in classification of Musa germplasm to distinguish individual accessions and in the development of a standardized procedure for genotyping. These markers can also be used in gene discovery and QTL mapping. The database represents a source of markers for developing and implementing new approaches for molecular breeding, which are required to enhance banana productivity.展开更多
Understanding the performance of new crop genotypes for traits of relevance is important in selecting potential cultivars to satisfy end-users. The objective of this study was to determine the performance of new banan...Understanding the performance of new crop genotypes for traits of relevance is important in selecting potential cultivars to satisfy end-users. The objective of this study was to determine the performance of new banana genotypes for bunch mass (BMS) and BMS-related traits, resistance to black Sigatoka and sensory attributes. Eight cooking banana genotypes consisting of six new hybrid genotypes selected from advanced breeding trials and two control cultivars were evaluated in a randomized complete block design for three crop cycles at three locations in Uganda. Genotype, location, crop cycle and their interaction effects were significantly different for most traits assessed. The overall top two genotypes (“9058K-2” and “8099K-16”) combining high BMS, resistance to black Sigatoka and acceptable sensory attributes were identified. These genotypes are suggested as potential commercial cultivars for release to farmers in Uganda and/or other East African countries with similar environmental conditions to those where the genotypes were tested. It should be noted that high BMS/yield and resistance to diseases are not the only factors to consider when selecting banana genotypes that meet end-user needs. A combination of high BMS/yield, acceptable sensory attributes and resistance to diseases essentially influence the selection process of banana cultivars.展开更多
采用AFLP技术,对35个香牙蕉品种(系)进行了鉴别与分类。从AFLP试剂盒所提供的64对引物组织中选取两对引物组合EcoR I ACC+Mse I CAT和EcoR IACC+Mse I CAG对35份香牙蕉材料进行了AFLP分析,共得到扩增位点107个,其中多态性位点84个...采用AFLP技术,对35个香牙蕉品种(系)进行了鉴别与分类。从AFLP试剂盒所提供的64对引物组织中选取两对引物组合EcoR I ACC+Mse I CAT和EcoR IACC+Mse I CAG对35份香牙蕉材料进行了AFLP分析,共得到扩增位点107个,其中多态性位点84个,多态性位点比例达到78.5%,对35个品种的区分率达到100%。各品种(系)多态性带数存在差别,多态性带数量多为海南红蕉(56条),多态性比例最高为0.6667,可见其杂合程度较高;多态性带最少的为大丰1-1号(33条),其多态性比例为0.3929。各品种(系)相互之间的相似系数介于0.71-1.00,表明香牙蕉品种(系)之间遗传关系相对较近,依相似系数0.88的水平,可以将供试的35个香牙蕉品种(单株)分为6个品种群,鉴别了3个引自不同国家的品种。几内亚、苹果以及阳江矮实际上为同一个香牙蕉品种,该研究结果基于AFLP分子标记,能从分子水平上反映香牙蕉地方品种之间的亲缘关系,可以作为香牙蕉品种(系)分类的依据。展开更多
文摘Banana streak virus (BSV) and Sugarcane bacilliform virus (SCBV) are two badnaviruses commonly found in all banana growing areas of the world. It is a threat to the production and improvement of Musa germplasm. In Burkina Faso, the presence of badnaviruses was reported in banana producing regions. The objective of this study was to determine the prevalence of BSV and SCBV in banana production areas of Burkina Faso. A survey followed by a symptomatologic study was conducted in banana plantations in 27 localities of the nine main banana producing regions from July to October 2018 and September to December 2020. In all, 251 leaf samples were collected and analysed for BSV and SCBV infection by Indirect Antigen Coated Plate Assay-ELISA followed by amplification of the RT/RNase H region using Polymerase chain reaction with Badna FP/RP and SCBV F/R primers, respectively. A variety of symptoms were observed on almost all plant organs which were revealed due to BSV by symptomatologic study. The results of serological and molecular diagnosis revealed a high overall prevalence of BSV in 80.48% of the samples tested. BSV was distributed in seven survey regions out of nine with prevalence ranging from 10% to 100% in North, Centre, Centre West, Hauts Bassins, Cascades, Centre East and Boucle of Mouhoun regions. Very low prevalence was recorded for SCBV in Cascades and East Centre region with 4.35 and 12.5%, respectively. Species detection using specific primers to each species revealed three main species: Banana streak Obino l’ewaï virus (BSOLV), Goldfinger virus (BSGFV) and Imové virus (BSIMV) in the samples tested, respectively in the proportions of 23%, 8% and 0.8%. Co-infection between BSV species was also detected.
文摘香蕉品种‘Agbagaba’和‘Orishele’的胚性细胞悬浮系(ECS)在液体培养基中分别预培养1和2周后,将其接种在RD1和M3培养基上,于光照或黑暗条件下进行体胚的再生。从沉积细胞体积(SCV) 为1 mL(1 mL SCV)的ECS获得的再生体胚数量因预培养时间、再生培养基的种类及培养条件的不同而异。植株的再生率及从1 mL SCV的ECS获得的再生植株数量也受上述体胚再生条件的间接影响。
文摘植物光系统Ⅰ反应中心亚基Ⅴ(photosystemⅠreaction center subunitⅤ,简称PSAG或PSⅠ-G)是光合系统Ⅰ的主要组件,具有维持PSⅠ复合体稳定性的重要作用,并与抗盐密切相关。本研究以福州宦溪野生蕉(Musa spp.AB group)叶片为材料,采用同源克隆的方法 ,首次分离到PSAG基因的2个成员:PSAG1、PSAG2(GenBank登录号分别为JX317082、JX317083),分别为800、827 bp,分别编码150、160个氨基酸;PSAG1、PSAG2的基因组序列分析表明2个成员均没有内含子。生物信息学分析表明:PSAG1、PSAG2具有PSⅠ的Ⅹ亚基超家族(photosystemⅠreaction center subunitⅩpsaK)保守结构域,是不具有信号肽的跨膜蛋白,具有亲水性;PSAG1、PSAG2均有4个位点发生磷酸化。宦溪野生蕉PSAG在进化过程中形成了特殊的结构特征,即PSAG1和PSAG2没有内含子,并且在不同物种间保守区具有高度的一致性,为保持PSAG功能的稳定性提供了重要保证。
文摘The center of diversity of the genus Musa (Musaceae) is in Southeast Asia, a region not studied in detail and where new species and varieties continue to be reported. A new wild banana species, M. chunii Hakkinen from Yunnan, China is described and illustrated based on observed morphological characteristics in the field. This extremely rare new species was only found in Tongbiguan Nature Reserve, Dehong District, West Yunnan. A key to M. chunii and related taxa is provided. In addition, critical notes regarding M. rubra Kurz identity are given.
基金Supported by the Research University Grant Scheme of Tier 1 provided by Universiti Teknologi Malaysia(Grant No.PY/2014/03649)
文摘Objective:To study the jelly formulation produced by Musa acuminata Colla(AAA Group) peels and evaluate its antioxidant properties which are related to the product quality.Methods:The formulations of peel jelly were established under two-level full factorial designs within two blocks and one center point.Regarding response optimizer,the amount of sugar and citric acid was obtained;hence,the peel jellies were produced.The evaluation of antioxidant properties was conducted by using total phenolic content(TPC)assay and 1,1 diphenyl-2-picrylhydrazyl(DPPH) free radical assay.Results:The TPC of peel powder varied from 91.8 to 602.26 mg gallic acid equivalents/100 g dry weight,and 5%-7% peel jellies had phenolic content ranging from 29.38 to 48.31 mg gallic acid equivalents/100 g dry weight.The results of DPPH test indicated that at 10 mg/mL,the peel powder showed 89% DPPH inhibition,while 7% peel jelly prominently exhibited 84% DPPH inhibition.The correlation between DPPH IC50 value and TPC of peel powder as well as peel jelly was quite reasonably high with correlation coefficient ranging from 0.843 7 to 0.995.Conclusions:TPC can be used as an indicator in assessing the antioxidant activity of fruits and vegetables.The present investigation reveals that TPC is mainly responsible for DPPH free radical scavenging capacity.
文摘Since the initial description, the name Musa aurantiaca Baker (1893) has been unclear to most botanists. The aim of this study is to settle its true identity and to update the description. The plant is distributed in the regions of Upper Assam and Arunachal Pradesh, India, Northern Myanmar and Tibet, China where it occurs commonly but it is not mentioned in Chinese literature at all. In this paper, the authors also review the description and the literature history of M. aurantiaca from 1893 to the present. Musa aurantiaca Baker is typified here.
基金Project (No. 30471112) supported by the National Natural Science Foundation of China
文摘Objective:Chilling tolerance of salicylic acid(SA) in banana seedlings(Musa acuminata cv.,Williams 8818) was investigated by changes in ultrastructure in this study.Methods:Light and electron microscope observation.Results:Pretreatment with 0.5 mmol/L SA under normal growth conditions(30/22 °C) by foliar spray and root irrigation resulted in many changes in ultrastructure of banana cells,such as cells separation from palisade parenchymas,the appearance of crevices in cell walls,the swelling of grana and stromal thylakoids,and a reduction in the number of starch granules.These results implied that SA treatment at 30/22 °C could be a type of stress.During 3 d of exposure to 7 °C chilling stress under low light,however,cell ultrastructure of SA-pretreated banana seedlings showed less deterioration than those of control seedlings(distilled water-pretreated).Conclusion:SA could provide some protection for cell structure of chilling-stressed banana seedling.
文摘Since the initial description, the name Musa dasycarpa Kurz (1867) has been unclear to most botanists. It has usually been synonymized with M. velutina H. Wendl. & Drude (1875). However, although the original diagnosis was very short, 'fruits hairy', it is adequate. Thus, according to Vienna Codes, M. dasycarpa Kurz has priority over M. velutina H. Wendl. & Drude. The aim of this study is to settle the true identity and to update the description of M. dasycarpa Kurz. For that purpose the names M. dasycarpa and M. velutina are typified. In addi-tion, critical notes regarding M. assamica Bull. are given; it is neotypified here, and considered as conspecific with M. sanguinea Hook. f.
文摘Organic agriculture promotes disease suppression through healthy soils by increasing biological activity and diversity through the application of organic fertilizers and increasing organic inputs. Fusarium wilt of bananas (Fusarium oxysporum f. sp. Fcubense) (Foc), also known as Panama disease, has been a devastating disease throughout the world. So far, no fungicides or cultural measures have been found that control Foc sufficiently. The aim of this research was to assess whether organic-based farming systems were more resilient than inorganic farming systems to soil borne diseases, in particular Fusarium wilts. A survey was conducted comparing five organic and five conventional banana plantations at paired sites in north Queensland, Australia. Soil samples were collected and analysed for chemical, physical and biological soil health indicators. Disease development of F. oxysporum f. sp. lycopersici in tomatoes and Foc in bananas were studied in pot trials to pursue clues for identifying Fusarium suppressive soil traits. Organic soils from the survey showed higher microbial activity and lower disease symptom expression (both with tomatoes and bananas) than conventional soils. In the survey, nematode diversity and soil sulphate content were recurring indicators in all experiments showing close correlations to pathogen growth, disease expression and plant health. Organic soils were lower in plant-parasitic nematodes and sulphate sulphur levels and higher in nematode diversity, labile soil C and microbial indicators. Soil conduciveness or suppression of Foc appeared to be largely governed by competition for carbon. Measurement of soil microbial enzyme activity, nematode community structure and diversity and possibly sulphate sulphur seem to provide a relatively reliable indicator for general disease suppression. Differences between organic and conventional agriculture cannot be related to single management practices, but may be linked to synergies among system components.
基金Malaysian Ministry of Higher Education(MOHE) for their sponsorship in this study and the HUM Endowment Fund for financial support to carry out this work
文摘Objective:To investigate the potential of Musa x paradisiaca(M.x paradisiaca)flower extracts in promoting milk production of lactating rats and its effects on growth of the suckling pups. Methods:Galactagogue activity was evaluated in terms of quantity of milk produced from the rats treated with petroleum ether,ethanol or water extracts of the flower.Lactating rats(n=5) of Spraque Dawley with six pups each were administered with the extracts in the amount of 500 mg/kg body weight,while the control rats were given an equivalent amount of distilled water.The rats were daily administered via oral feeding starting from Day 5 until Day 14 and the performance of milk production was measured along the experimental period by weight-suckle-weight method. Results were statistically analyzed using SPSS by means of ANOVA at 0.05 and was expressed as their mean±standard deviation.The rates of pups’ growth were measured as the weight gain along the experimental period.Results:The rats treated with aqueous extract produced higher milk than control and ethanol groups.Aqueous extract was identified to increase milk production by 25%,while petroleum ether extract by 18%.The mean of yields produced by the rats during suckling period for aqueous,petroleum ether,ethanol and control were 4.62±2.45,4.37±1.93,3.65±1.89 and 3.69±1.79,respectively.Growth rates of pups for the rats treated with control,aqueous, ethanol extract and petroleum ether were(1.85±0.49),(1.78±0.56),(1.65±0.46)and(1.56±0.42) g/pup, respectively.Conclusions:The present study reveals the potential of M.x paradisiaca flower to enhance milk production of nursing mothers which could be exploited for commercialization of the isolated extract.
文摘The genus Musa is one of three genera in the family Musaceae, which includes bananas and plantains, which are monocotyledonous plants. Bananas have valuable nutritional content of vitamin C, B6, minerals, and dietary fiber and are a rich food energy source, given that carbohydrates account for 22%–32% of fruit weight. Molecular markers are valuable for crop improvement and population genetics studies. The availability of whole-genome sequence and in silico approaches has revolutionized bulk marker discovery. We describe an online web genomic resource, BanSatDB(http://webtom.cabgrid.res.in/bansatdb/) having the highest number(>341,000) of putative STR markers from Musa genera so far, represented by three species: M. acuminata(110,000), M. balbisiana(107,000), and M. itinerans(124,000)from 11 chromosomes of each species. BanSatDB has also been populated with 580 validated STR markers from the published literature. It is based on a three-tier architecture using MySQL, PHP and Apache. The markers can be retrieved by use of multiple search parameters including chromosome number(s), microsatellite types(simple or compound),repeat nucleotides(1–6), copy number, microsatellite length, pattern of repeat motif, and chromosome location. These markers can be used for Distinctness, Uniformity and Stability(DUS) tests of variety identification and for marker assisted selection(MAS) in variety improvement and management. These STRs have also proved to be helpful in classification of Musa germplasm to distinguish individual accessions and in the development of a standardized procedure for genotyping. These markers can also be used in gene discovery and QTL mapping. The database represents a source of markers for developing and implementing new approaches for molecular breeding, which are required to enhance banana productivity.
文摘Understanding the performance of new crop genotypes for traits of relevance is important in selecting potential cultivars to satisfy end-users. The objective of this study was to determine the performance of new banana genotypes for bunch mass (BMS) and BMS-related traits, resistance to black Sigatoka and sensory attributes. Eight cooking banana genotypes consisting of six new hybrid genotypes selected from advanced breeding trials and two control cultivars were evaluated in a randomized complete block design for three crop cycles at three locations in Uganda. Genotype, location, crop cycle and their interaction effects were significantly different for most traits assessed. The overall top two genotypes (“9058K-2” and “8099K-16”) combining high BMS, resistance to black Sigatoka and acceptable sensory attributes were identified. These genotypes are suggested as potential commercial cultivars for release to farmers in Uganda and/or other East African countries with similar environmental conditions to those where the genotypes were tested. It should be noted that high BMS/yield and resistance to diseases are not the only factors to consider when selecting banana genotypes that meet end-user needs. A combination of high BMS/yield, acceptable sensory attributes and resistance to diseases essentially influence the selection process of banana cultivars.
文摘采用AFLP技术,对35个香牙蕉品种(系)进行了鉴别与分类。从AFLP试剂盒所提供的64对引物组织中选取两对引物组合EcoR I ACC+Mse I CAT和EcoR IACC+Mse I CAG对35份香牙蕉材料进行了AFLP分析,共得到扩增位点107个,其中多态性位点84个,多态性位点比例达到78.5%,对35个品种的区分率达到100%。各品种(系)多态性带数存在差别,多态性带数量多为海南红蕉(56条),多态性比例最高为0.6667,可见其杂合程度较高;多态性带最少的为大丰1-1号(33条),其多态性比例为0.3929。各品种(系)相互之间的相似系数介于0.71-1.00,表明香牙蕉品种(系)之间遗传关系相对较近,依相似系数0.88的水平,可以将供试的35个香牙蕉品种(单株)分为6个品种群,鉴别了3个引自不同国家的品种。几内亚、苹果以及阳江矮实际上为同一个香牙蕉品种,该研究结果基于AFLP分子标记,能从分子水平上反映香牙蕉地方品种之间的亲缘关系,可以作为香牙蕉品种(系)分类的依据。