Under the prerequisite that the incidence of cancer or tumor in negative-control nude mice inoculated subcutaneously with primary feline or canine kidney cell cultures purified in vitro at passage 3 was 0 (0/22) and 0...Under the prerequisite that the incidence of cancer or tumor in negative-control nude mice inoculated subcutaneously with primary feline or canine kidney cell cultures purified in vitro at passage 3 was 0 (0/22) and 0 (0/10), respectively. The incidence of the progressively-growing malignant tumor(MT) in positive-control nude mice inoculated subcutaneously with Hela cell cultures of KB, X, or NM20/X strain was 10/ 10, 25/25 and 5/51, respectively. The results showed that the incidence of tumor in nude mice with di-and hy-perploid YB strain of MDCK cell during 17 - 23 passages, with hyper- and hypoploid KA strain of MDCK cell during 6-8 passages, with hypoploid WB strain of MDCK cell on passage 6, with hyper-and hypoploid H strain of MDCK cell during 8-24 passages was 2/24, 6/10, 5/10 and 10/15, respectively. The chromosomal analysis results showed that the ratio of difference in the rate of modal chromosome number between high(mcs + n) and lowest (mcs)passages was not more than 5 - 15% and the structure aberrations was generally 0 -3 %. These results proved that the genetic characteristics of chromosomal number of cell lines determines their tumorigenicity, but it is species-specific. MDCK line has tumorigenicity no matter what its chromosome kary-otype is, at least it has very low tumorigenicity even when its modal chromosome number is hypoploid. It is thus evident that MDCK cell of WB or H strain can be approved as substrate for the preparation of attenuated viral vaccines, but MDCK cell of YB or KA strain can not be approved as substrate for the preparation of attenuated viral vaccines.展开更多
In the present paper the attention is focused on effect of arrangement of tube banks on acoustic resonance which occurred in the two-dimensional model of boiler. We have examined the characteristics of vortex shedding...In the present paper the attention is focused on effect of arrangement of tube banks on acoustic resonance which occurred in the two-dimensional model of boiler. We have examined the characteristics of vortex shedding and acoustic resonance generated from in-line and staggered tube banks. At the small tube pitch ratio in in-line tube banks, acoustic resonance of third and fourth mode in the transverse direction occurred. As the tube pitch ratio in the flow direction decreased, the vortex shedding frequency became broad-band. The alternative vortex shed from in-line tube banks. The multiple resonance modes were generated within the broad-band vortex shedding frequency. And the acoustic resonances of lower-order modes occurred at the higher gap velocity. On the other hand, at the small tube pitch ratio in staggered tube banks, acoustic resonance did not occurred, although the vortex shed at the resonance frequency in tube banks. The pressure drop at staggered tube banks was larger than that of in-line tube banks. The symmetric vortices were observed inside staggered tube banks at the small tube pitch ratio.展开更多
文摘Under the prerequisite that the incidence of cancer or tumor in negative-control nude mice inoculated subcutaneously with primary feline or canine kidney cell cultures purified in vitro at passage 3 was 0 (0/22) and 0 (0/10), respectively. The incidence of the progressively-growing malignant tumor(MT) in positive-control nude mice inoculated subcutaneously with Hela cell cultures of KB, X, or NM20/X strain was 10/ 10, 25/25 and 5/51, respectively. The results showed that the incidence of tumor in nude mice with di-and hy-perploid YB strain of MDCK cell during 17 - 23 passages, with hyper- and hypoploid KA strain of MDCK cell during 6-8 passages, with hypoploid WB strain of MDCK cell on passage 6, with hyper-and hypoploid H strain of MDCK cell during 8-24 passages was 2/24, 6/10, 5/10 and 10/15, respectively. The chromosomal analysis results showed that the ratio of difference in the rate of modal chromosome number between high(mcs + n) and lowest (mcs)passages was not more than 5 - 15% and the structure aberrations was generally 0 -3 %. These results proved that the genetic characteristics of chromosomal number of cell lines determines their tumorigenicity, but it is species-specific. MDCK line has tumorigenicity no matter what its chromosome kary-otype is, at least it has very low tumorigenicity even when its modal chromosome number is hypoploid. It is thus evident that MDCK cell of WB or H strain can be approved as substrate for the preparation of attenuated viral vaccines, but MDCK cell of YB or KA strain can not be approved as substrate for the preparation of attenuated viral vaccines.
文摘In the present paper the attention is focused on effect of arrangement of tube banks on acoustic resonance which occurred in the two-dimensional model of boiler. We have examined the characteristics of vortex shedding and acoustic resonance generated from in-line and staggered tube banks. At the small tube pitch ratio in in-line tube banks, acoustic resonance of third and fourth mode in the transverse direction occurred. As the tube pitch ratio in the flow direction decreased, the vortex shedding frequency became broad-band. The alternative vortex shed from in-line tube banks. The multiple resonance modes were generated within the broad-band vortex shedding frequency. And the acoustic resonances of lower-order modes occurred at the higher gap velocity. On the other hand, at the small tube pitch ratio in staggered tube banks, acoustic resonance did not occurred, although the vortex shed at the resonance frequency in tube banks. The pressure drop at staggered tube banks was larger than that of in-line tube banks. The symmetric vortices were observed inside staggered tube banks at the small tube pitch ratio.
