A reserved-phase HPLC method was developed for the determination of barbaloin in Aloe vera L. var. chinensis (Haw.) Berger and Aloe barbadensis Miller, and whether there was a close relationship between the contents o...A reserved-phase HPLC method was developed for the determination of barbaloin in Aloe vera L. var. chinensis (Haw.) Berger and Aloe barbadensis Miller, and whether there was a close relationship between the contents of barbaloin and their environments in which they were growing was decided. A Hypersil ODS column (4.6 mm×200 mm, 5 μm)was used with a mobile phase of methanol-water (40:60, containing 0.1% acetic acid), the flow rate being 1.0 mL·min -1, detection wavelength at 359 nm, and the column temperature being 30℃. The linear range of barbaloin was between 0.0726 and 0.726 μg with a correlation coefficient of 0.9998 and the regression equation being Y=1.9202×10 6X-1801.9. Barbaloin was stable in methanol in 48 h and the instrument precision was 1.2% while the method precision was 4.9%. The contents of barbaloin of 12 samples ranged from 6.160 to 319.1 μg·g -1. The method developed was fast and simple with good reproducibility. There was high correlation between the contents of barbaloin and their growing environments.展开更多
Barbaloin is C-glucoside of aloe emodin unthrone which is found in the plant name as Aloe vera is a perennial succulenl(Liliaceal),also called the healing planl.Barbaloin have variety of pharmacological activity such ...Barbaloin is C-glucoside of aloe emodin unthrone which is found in the plant name as Aloe vera is a perennial succulenl(Liliaceal),also called the healing planl.Barbaloin have variety of pharmacological activity such as strong inhibitory effect on histamine release,anti-inflammatory,cathartic,antiviral,antimicrobial,anticancer,antioxidant activity and alternative for pharmaceutical or cosmetic applications.The peak amount of barbaloin was reached about 3 h after oral administration.Concentration of barbaloin in Aloe vera leaves was shown to depend on the leaf part,age,and position of the leaf.Young leaves contain more barbaloin compared to old one.Various researches have been done on barbaloin but still the relationship between the barbaloin and its overall effect has not been clarified.A more specific;perceptive of the pharmacological activities of barbaloin is required to develop for pharmaceutical purpose.Many attempts have been made regarding its isolation,biological activity to examine their effects,and clarify their functional mechanism.This review gives a brief idea about its uses,ethnomedicinally and commercially important analytical techniques and their pharmacological activities.展开更多
A multidisciplinary approach-anatomy, histochemistry and phytochemistry-was used to investigate the leaf structure, the content and the storage location of barbaloin in the leaves of Aloe vera L. var. chinensis (Haw.)...A multidisciplinary approach-anatomy, histochemistry and phytochemistry-was used to investigate the leaf structure, the content and the storage location of barbaloin in the leaves of Aloe vera L. var. chinensis (Haw.) Berg. Xeromorphic characteristics including secondary thickened epidermal cell walls, thicker cuticle, ambiguous differentiation of spongy and palisade tissues in the chlorenchyma, and well-developed aquiferous tissue could be seen in the leaves. Several large parenchymatous cells were observed at the phloem pole of the first ring of vascular bundles. The secondary ring of vascular bundles in the leaf base and the stomata, which are surrounded by five cells, have some classification significance in this species. The density of vascular bundles, the content of barbaloin and the intensity of histochemical reaction differed among leaf numbers Ll (annual leaf), L2 (biennial leaf), L3 (triennial leaf) and L4 (quadrennial leaf), and in different parts of the leaf. These three factors were highest in the youngest leaf, Ll, and top parts of all the leaves and lowest in the basal parts and the oldest leaf, L4. The density of vascular bundles had a positive correlation to the content of barbaloin. The histochemical results revealed that the small sheath cells that surrounded the bundles might be the location of barbaloin synthesis and the large parenchymatous cells beneath the sheath might be the storage places of this metabolite.展开更多
文摘A reserved-phase HPLC method was developed for the determination of barbaloin in Aloe vera L. var. chinensis (Haw.) Berger and Aloe barbadensis Miller, and whether there was a close relationship between the contents of barbaloin and their environments in which they were growing was decided. A Hypersil ODS column (4.6 mm×200 mm, 5 μm)was used with a mobile phase of methanol-water (40:60, containing 0.1% acetic acid), the flow rate being 1.0 mL·min -1, detection wavelength at 359 nm, and the column temperature being 30℃. The linear range of barbaloin was between 0.0726 and 0.726 μg with a correlation coefficient of 0.9998 and the regression equation being Y=1.9202×10 6X-1801.9. Barbaloin was stable in methanol in 48 h and the instrument precision was 1.2% while the method precision was 4.9%. The contents of barbaloin of 12 samples ranged from 6.160 to 319.1 μg·g -1. The method developed was fast and simple with good reproducibility. There was high correlation between the contents of barbaloin and their growing environments.
文摘Barbaloin is C-glucoside of aloe emodin unthrone which is found in the plant name as Aloe vera is a perennial succulenl(Liliaceal),also called the healing planl.Barbaloin have variety of pharmacological activity such as strong inhibitory effect on histamine release,anti-inflammatory,cathartic,antiviral,antimicrobial,anticancer,antioxidant activity and alternative for pharmaceutical or cosmetic applications.The peak amount of barbaloin was reached about 3 h after oral administration.Concentration of barbaloin in Aloe vera leaves was shown to depend on the leaf part,age,and position of the leaf.Young leaves contain more barbaloin compared to old one.Various researches have been done on barbaloin but still the relationship between the barbaloin and its overall effect has not been clarified.A more specific;perceptive of the pharmacological activities of barbaloin is required to develop for pharmaceutical purpose.Many attempts have been made regarding its isolation,biological activity to examine their effects,and clarify their functional mechanism.This review gives a brief idea about its uses,ethnomedicinally and commercially important analytical techniques and their pharmacological activities.
文摘A multidisciplinary approach-anatomy, histochemistry and phytochemistry-was used to investigate the leaf structure, the content and the storage location of barbaloin in the leaves of Aloe vera L. var. chinensis (Haw.) Berg. Xeromorphic characteristics including secondary thickened epidermal cell walls, thicker cuticle, ambiguous differentiation of spongy and palisade tissues in the chlorenchyma, and well-developed aquiferous tissue could be seen in the leaves. Several large parenchymatous cells were observed at the phloem pole of the first ring of vascular bundles. The secondary ring of vascular bundles in the leaf base and the stomata, which are surrounded by five cells, have some classification significance in this species. The density of vascular bundles, the content of barbaloin and the intensity of histochemical reaction differed among leaf numbers Ll (annual leaf), L2 (biennial leaf), L3 (triennial leaf) and L4 (quadrennial leaf), and in different parts of the leaf. These three factors were highest in the youngest leaf, Ll, and top parts of all the leaves and lowest in the basal parts and the oldest leaf, L4. The density of vascular bundles had a positive correlation to the content of barbaloin. The histochemical results revealed that the small sheath cells that surrounded the bundles might be the location of barbaloin synthesis and the large parenchymatous cells beneath the sheath might be the storage places of this metabolite.
