Analysis of Patent Application Status of Scutellaria Barbata Industrial Chain Based on Patent Data

Objective To analyze patent application status of Scutellaria Barbata industrial chain and provide some suggestions for its patent application and product development.Methods Patent data were collected through IncoPat patent analysis system.Meanwhile,the patent analysis method combined with text mining method was adopted to analyze the situation and development trend of patent application in China’s Scutellaria Barbata industrial chain by using pie chart,bubble chart,trend chart and other visual charts to display the results.Results and Conclusion The patent application of Scutellaria Barbata in China mainly experienced three stages:Slow development,rapid development,and recession period.The number of patents is large,but the authorization rate is low.Individuals and enterprises are the main applicants for patent applications.Product development is involved in the whole industrial chain,but it basically focuses on its efficacy in downstream drugs,health food and other aspects.Therefore,government should enhance the awareness of patent protection,encourage collaborative innovation in industry-university-research to promote the combination of basic research and market application.Besides,it should provide theoretical support to tackle the problem of short board products,which can promote the transformation of scientific and technological achievements and contribute to the upgrading of Scutellaria Barbata industrial chain.

Scutellaria barbata attenuates diabetic retinopathy by preventing retinal inflammation and the decreased expression of tight junction protein

AIM： To observe the attenuation of ethanol extract of Herba Scutellaria barbata （SE） against diabetic retinopathy （DR） and its engaged mechanism. METHODS： C57BL/6J mice were intraperitoneally injected with streptozotocin （STZ, 55 mg/kg） for 5 consecutive days to induce diabetes, The diabetic mice were orally given with SE （100, 200 mg/kg） for lmo at lmo after STZ injection. Blood-retinal barrier （BRB） breakdown was detected by using Evans blue permeation assay. Real-time polymerase chain reaction （RT-PCR）, Western blot and immunofiuorescence staining were used to detect mRNA and protein expression. Enzyme-linked immunosorbent assay （ELISA） was used to detect serum contents of tumor necrosis factor-e （TNF-a） and interleukin （IL）-II. RESULTS： SE （100, 200 mg/kg） reversed the breakdown of BRB in STZ-induced diabetic mice. The decreased expression of retinal claudin-1 and claudin-19, which are both tight junction （T J） proteins, was reversed by SE. SE decreased the increased serum contents and retinal mRNA expression of TNF-a and IL-113. SE also decreased the increased retinal expression of intercellular cell adhesion molecule-1 （ICAM-1）. SE reduced the increased phosphorylation of nuclear factor kappa B （NFKB） p65 and its subsequent nuclear translocation in retinas from STZ- induced diabetic mice. Results of Western blot and retinal immunofluorescence staining of ionized calcium-binding adapter molecule 1 （Ibal） demonstrated that SE abrogated the activation of microglia cells in STZ-induced diabetic mice. CONCLUSION： SE attenuates the development of DR by inhibiting retinal inflammation and restoring the decreased expression of TJ proteins including claudin-1 and claudin-19.

Antitumor effects and compatibility mechanism of Hedyotis Diffusa-Sculellaria Barbata herb pair

OBJECTIVE Hedyotis diffusa Willd and Scutellaria barbata D Don are most commonly used herb pair for cancer treatment in traditional Chinese medicine.This study aimed to evaluated the in vitro and in vivo antitumor effects and the compatibility mechanisms of Hedyotis diffusa-Sculellaria barbata herb pair.METHODS Hedyotic diffusa extract,Scutellariae Barbatae extract,and herb pair extract were prepared and the component were detected by UPLC-Q-TOF-MS.The effects of different concentrations of Hedyotic diffusa extract,Scutellariae Barbatae extract,and herb pair extract were detected using MTS assay.The in vivo antitumor effects were evaluated in Panc28 pancreatic cells bearing nude mice model.The compatibility mechanism of Hedyotis diffusa-Sculellaria barbata herb pair were evaluated based on the network pharmacology,using TCMSP,Cytoscape 3.6.1 software,and DAVID 6.8.The anticancer mechanism were further validated in vitro.RESULTS Both the MTS and in vivo results showed that herb pair extract showed more obvious inhibitory effects on cancer cells compared to each individual.A total of 37 active components were selected from Hedyotis diffusa and Sculellaria barbata,33 kinds of active ingredients are involved in their anti-tumor effects.58 cancer-related targets and 66 KEGG pathways were identified.The potential targets for the herb pair might be prostaglandin G/H synthase 2(PTGS2),HSP90,EGFR,72 ku typeⅣcollagenase,PPAR-γ,et al.In vitro validation result showed that compatibility mech⁃anisms was related with HSP90,EGFR related pathways.CONCLUSION The result of the study preliminarily verified the basic anti-tumor pharmacological effects of Hedyotis diffusa-Sculellaria barbata herb pair,and lays a solid foundation for further studies on the anti-tumor mechanism of the herbal pair.

Attenuation of Scutellaria barbata flavonoids on cortical cytoplasm apoptotic factors disorders induced by complex Aβ_(25-35) in rats

OBJECTIVE To investigate the effect of SBF on cortical cytoplasm apoptotic factors disturbances induced by complex Aβ25-35in rats.METHODS The cerebral injuries model was established by rats received intracere-broventricular injection of RHTGF-β1,Aβ25-35and Al Cl3and then accepted SBF treatment.All the rats were sacrificed by decapitation for indicators detection at last the drug treatment.Western blotting method was for caspase-3 protein expression and RT-PCR method detected cytochrome c,apoptotic protease activating factor-1(Apaf-1),caspase-9 m RNA expression in cortical cytoplasm.RESULTS The protein expression of caspase-3in cortical cytoplasm of rats was assayed by Western blotting.The results indicated that compared with the sham group,the caspase-3 protein expression of cortical cytoplasm in Aβgroup was significantly increased(P<0.01).However,the increased expression can be obviously reversed by SBF at doses of 35,70 and 140mg·kg-1,as compared with model group(P<0.01).The Cyt-C,Apaf-1 and caspase-9 m RNA expressions in cortical cytoplasm of rats were determined by RT-PCR.The results indicated that compared with the sham group,the Cyt-C Apaf-1 and caspase-9 m RNA relative expressions of cortical cytoplasm in Aβgroup was significantly increased(P<0.01).However,these increased expressions can be differently reversed by SBF at doses of 35,70 and 140 mg·kg-1,as compared with model group(P<0.01).CONCLUSION SBF can definitely improve rats′cortical cytoplasm apoptotic factors disorders induced by complex Aβ25-35,which maybe benefit for treatment of degenerative disease.

半枝莲抗肿瘤的信号通路研究进展

半枝莲在中国药用历史悠久,经现代研究证实含有黄酮类、多糖类、二萜类等多种抗肿瘤有效成分。梳理近年来半枝莲及其活性成分靶向肿瘤的各个信号通路的相关文献发现,半枝莲及其活性成分通过调控磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)、酪氨酸蛋白激酶/信号转导和转录激活因子(JAK/STAT)、Wnt/β-连环蛋白(Wnt/β-cate-nin)、丝裂原活化蛋白激酶(MAPK)、半胱氨酸蛋白酶(Caspase)途径、核因子-κB(NF-κB)、蛋白53(p53)和Hedgehog等多条信号通路,发挥抑制肿瘤细胞增殖、诱导肿瘤细胞凋亡、降低迁移和侵袭能力、抗肿瘤血管形成、调节机体的免疫功能等作用,达到抗肿瘤效应。广泛应用于结肠癌、肺癌、肝癌、乳腺癌等多种肿瘤疾病的治疗。研究以细胞信号通路为切入点,总结半枝莲抗肿瘤的作用机制,以期为深入挖掘半枝莲抗肿瘤机制及其临床应用提供参考。

Two New neo-Clerodane Diterpenoids from Scutellaria barbata

Two new neo-clerodane diterpenoids, 6,7-dibenzoyloxybarbatin C （1, named barbatin D） and 6-（2-acetoxy-3-methylbutanoloxy）-7-（2-carbonyl-3-methylbutanoyloxy） barbatin C （2, named barbatin E） were isolated from the whole plant of Scutellaria barbata D. Don. Their structures were elucidated by spectroscopic methods including extensive 1 D and 2D NMR analyses. In vitro, compounds 1-2 showed cytotoxic activities against three human cancer lines, namely, HONE-1 nasopharyngeal, KB oral epidermoid carcinoma, and HT29 colorectal carcinoma cells, and with IC50 values in the range of 3.5-6.7μM.

Scutellaria barbata D.Don Inhibits Colorectal Cancer Growth via Suppression of Wnt/β-Catenin Signaling Pathway

Objective： To investigate the effect of the ethanol extract of Scutellaria barbata D. Don（EESB） on colorectal cancer（CRC） growth and Wnt/β-catenin signaling pathway in vivo and in vitro. Methods： In vivoexperiment, CRC xenograft mouse model was constructed with injection of HT-29 cells. Following xenograft implantation, twenty mice were randomly divided into EESB-treated group（n=10） and control group（n=10） by a random number table, and were given with intra-gastric administration of 2 g/kg EESB or saline, 5 days a week for 16 days, respectively. At the end of experiment, tumors were removed and weighed by electronic scales. The proliferation biomarker Ki-67 of tumor was evaluated by immunohistochemistry（IHC） assay. In vitro study, HT-29 cells were treated with 0, 0.5, 1.5, 2.5 mg/m L EESB for 24 h. At the end of the treatment, the viability and survival of HT-29 cells were determined by methylthiazolyldiphenyl-tetrazolium bromide（MTT） assay and colony formation assay, respectively. The m RNA expression of c-Myc, Survivin and adenomatous polyposis coli（APC） was examined by reverse transcription-polymerase chain reaction（RT-PCR） both in tumor tissues of CRC xenograft mice and HT-29 cells. Protein expression of c-Myc, Survivin, APC, and β-catenin as well as β-catenin phosphorylation level were evaluated by IHC assay or Western blotting. Results： EESB significantly reduced tumor weight in CRC xenografts mice, compared with the control group（P〈0.05）. IHC assay showed that EESB significantly inhibited protein expression of Ki-67 in tumor tissues（P〈0.05）. MTT assay showed that EESB significantly reduced HT-29 cell viability in a dose-dependent manner（P〈0.05）. Colony formation assay showed that EESB dose-dependently decreased the survival of HT-29 cells（P〈0.05）. In addition, RT-PCR assay showed that EESB decreased the m RNA expression of c-Myc and Survivin and increased APC expression, both in tumor tissues of CRC xenograft mice and HT-29 cells（P〈0.05）. IHC assay or Western blotting showed that EESB decreased protein expression of β-catenin, c-Myc and Survivin, as well as increased APC expression and β-catenin phosphorylation in tumor tissues or HT-29 cells（P〈0.05）. Conclusions： EESB significantly reduced tumor growth in CRC xenografts mice, and inhibited the viability and survival of HT-29 cells. EESB could suppress the activation of the Wnt/β-catenin pathway, which might be one of the mechanisms whereby Scutellaria barbata D. Don exerts its anticancer activity.

半枝莲治疗恶性胸腔积液作用机制的网络药理及分子对接研究

目的通过网络药理和分子对接方法,探究半枝莲治疗恶性胸腔积液(malignant pleural effusion,MPE)的作用机制。方法利用IBW SPSS Modeler 18.0软件对绍兴第二医院中医科门诊治疗MPE处方信息进行数据挖掘,使用中药系统药理学数据库与分析平台筛选半枝莲有效活性成分,利用GeneCards、OMIM、DisGeNET数据库获取MPE疾病靶点,通过Cytoscape3.9.1软件构建“中药-成分-靶点”网络图。利用DAVID在线平台生成基因本体(gene ontology,GO)功能富集分析图和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析图。使用PyMol和AutoDock vina软件将半枝莲有效活性成分与核心靶点蛋白进行分子对接验证。结果半枝莲治疗MPE的主要活性成分有18种,其中黄芩黄酮、3-甲基鼠李素、荠苧黄酮、黄芩素、木犀草素等为主要活性成分,EGFR、AKT1、HIF1A、SRC等为核心靶点蛋白。结论半枝莲通过多成分、多靶点作用于MPE,为进一步研发MPE治疗药物提供理论基础和新的切入点。

广西产半枝莲中黄酮类化学成分研究

目的:对广西产半枝莲的黄酮类成分进行研究。方法:综合利用正反相柱层析、MCI柱层析、HPLC等色谱分离技术对半枝莲全草进行提取分离,并通过波谱数据进行结构鉴定。结果:从半枝莲中共分离和鉴定了5个化合物,分别为白杨素(1)、芹菜素(2)、木犀草素(3)、千层纸素(4)、柚皮素(5)。结论:分离得到的化合物类型均为黄酮类化合物,可为广西半枝莲的黄酮成分分析提供一定的参考。

白花蛇舌草-半枝莲药对治疗胃癌的物质基础和作用机制研究进展

胃癌是消化系统的常见恶性肿瘤之一,好发于中老年人群。中医认为胃癌是由津亏热结、癌毒胶结于胃腹而形成的一种瘤瘕,在临床中常用清热解毒类药物进行治疗。白花蛇舌草-半枝莲药对具有清热解毒、活血化瘀、消痈散结、抗炎止痛的作用,可治疗胃癌。现代药理学研究证实,白花蛇舌草-半枝莲药对可通过诱导细胞凋亡、抑制细胞增殖、抑制血管生成、改善免疫微环境、下调端粒酶活性等途径起到抗胃癌的作用。本文对白花蛇舌草-半枝莲药对配伍后活性成分抗胃癌的机制进行综述,为白花蛇舌草-半枝莲药对的临床用药和抗胃癌新药研发提供理论依据和参考。

半枝莲含药血清对膀胱癌T24细胞凋亡和黏附力的影响探究

目的:探究半枝莲含药血清对膀胱癌T24细胞凋亡和黏附力的影响。方法:SPF级雄性Wistar大鼠20只,随机分为无药血清组、半枝莲低剂量组、半枝莲中剂量组、半枝莲高剂量组,每组5只,分别以蒸馏水及半枝莲1.5g/(kg·d)、3g/(kg·d)、6g/(kg·d)腹腔注射灌胃,连续3d。末次灌胃后抽取心主动脉血,经灭活处理及微孔过滤或获得半枝莲含药血清。体外培养膀胱癌T24细胞株,分为无药血清组、半枝莲(低、中、高剂量)组,分别对应各组动物血清,以无药血清及含10%的药物血清培养。使用CCK-8法检测细胞增殖情况;流式细胞仪检测各组细胞凋亡率及周期分布;MTT酶标仪法检测各药物浓度下细胞的黏附率;以蛋白印记免疫法检测凋亡相关蛋白[B-细胞淋巴瘤因子2(Bcl-2)抗体、BCL2关联X蛋白(Bax)抗体、胱天蛋白酶3(CASP3)]的表达。结果:与无药血清组比较,半枝莲低剂量组24h及48h的增殖抑制率及凋亡率、细胞G0/G1期、黏附率增加(P<0.05);与半枝莲低剂量组比较,半枝莲中剂量组24h及48h的增殖抑制率及凋亡率、细胞G0/G1期、黏附率增加(P<0.05);与半枝莲中剂量组比较,半枝莲高剂量组24h及48h的增殖抑制率及凋亡率、细胞G0/G1期、黏附率增加(P<0.05)。与无药血清组比较,半枝莲低剂量组Bcl-2相对表达量下降,Bax、CASP3相对表达量增加(P<0.05);与半枝莲低剂量组比较,半枝莲中剂量组Bcl-2相对表达量下降,Bax、CASP3相对表达量增加(P<0.05);与半枝莲中剂量组比较,半枝莲高剂量组Bcl-2相对表达量下降,Bax、CASP3相对表达量增加(P<0.05)。结论:半枝莲含药血清体外培养膀胱癌T24细胞,可有效抑制细胞增殖、降低黏附力,并能诱导细胞凋亡,具有作为抗膀胱癌药物研发的价值。

中药半枝莲质量评价的研究进展

目的了解中药半枝莲质量评价的研究进展。方法通过查阅相关文献并归纳总结,对半枝莲质量评价方面的研究进行综述。结果半枝莲质量评价的研究内容主要包括性状和显微鉴定、指纹图谱研究以及含量测定研究,其中对于有效部位和药材中单体成分的含量测定研究报道相对较多。结论半枝莲的质量评价研究取得了一定进展,但尚存在一些问题,需要进一步深入探讨。

半枝莲含药血清通过抑制mTOR信号通路影响非小细胞肺癌NCI-H1650细胞上皮间质转化

目的探讨半枝莲含药血清通过抑制雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)信号通路影响非小细胞肺癌(non-small cell lung cancer,NSCLC)NCI-H1650细胞上皮间质转化。方法选取健康青紫蓝大白兔制备半枝莲含药血清,根据不同剂量分为对照组、低剂量组(A组)、中剂量组(B组)、高剂量组(C组)。选取人非小细胞肺癌细胞NCI-H1650与含药血清培养,采用逆转录RT-PCR、免疫印迹、MTT、划痕实验等方法,观察4组不同剂量半枝莲含药血清抑制mTOR信号通路对NCI-H1650细胞上皮间质转化、增殖、迁移的影响。结果4组mTOR表达量差异具有统计学意义,其中C组mTOR表达量低于对照组和A组(P<0.05);4组Vimentin、N-cadherin、E-cadherin蛋白表达相对灰度值差异具有统计学意义,且C组Vimentin、N-cadherin灰度值低于对照组、A组、B组,E-cadherin灰度值高于对照组(P<0.05);4组24、48、72 h吸光度差异具有统计学意义,且C组24、48、72 h吸光度低于对照组、A组、B组(P<0.05);4组NCI-H1650细胞迁移率差异具有统计学意义,且A组、B组、C组迁移率低于对照组,C组低于A组、B组(P<0.05)。结论高剂量半枝莲含药血清可通过抑制mTOR信号通路影响NCI-H1650细胞上皮间质转化、增殖及迁移,从而抑制NSCLC发生和发展。

近十年半枝莲抗肿瘤作用机制研究概况

半枝莲在调控肿瘤细胞的生长、侵袭转移、免疫功能和抗化疗药物耐药等方面发挥重要的作用,具有多成分、多效应、多途径、多靶点和毒副作用小的优势。半枝莲在临床中是一种极具开发潜力的药用资源,具有广阔的应用前景。本文现从抑制肿瘤细胞增殖,阻滞细胞生长周期,诱导细胞凋亡,促进细胞自噬,抑制血管生成,抑制细胞迁移和侵袭,调节免疫、抗化疗药物耐药等药理作用机制着手,对近十年的国内外关于半枝莲抗肿瘤作用机制方面的研究进行综述,发现半枝莲可通过调控Wnt/胞内β-连锁蛋白(β-catenin)、microRNA、磷脂酰肌醇-3激酶(phosphatidylinositol-3-kinases,PI3K)/蛋白激酶B(protein kinase B,Akt)和刺猬因子(sonic hedgehog,SHH)相关信号通路抑制肿瘤细胞生长。此外,半枝莲还能通过抑制肿瘤细胞运动相关蛋白和细胞因子,调节肿瘤细胞分解酶的活性及分解酶/分解酶抑制剂的平衡,发挥抑制细胞迁移和侵袭的作用,并能通过调节免疫相关细胞因子水平和抑制P-糖蛋白(P-glycoprotein,P-gp)的外排逆转化疗药物耐药。在今后的研究中有必要从转录组学、基因组学和蛋白组学等分子水平进一步探讨和验证其抗肿瘤作用机制,为临床应用和进一步新型靶向药物研究提供有力的证据支持。

半枝莲化学成分及其体外抑制乳腺癌细胞增殖活性

目的研究半枝莲Scutellaria barbata D.Don化学成分及其体外乳腺癌细胞增殖活性。方法半枝莲95%乙醇提取物采用聚酰胺、Sephadex LH-20、HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用MTT法对半枝莲各乙醇洗脱部位进行抗乳腺癌活性筛选,并评价各化合物对人乳腺癌细胞MDA-MB-231和人乳腺导管癌细胞株HCC38体外增殖的影响。结果从中分离得到9个化合物,分别鉴定为毛蕊花糖苷(1)、异毛蕊花糖苷(2)、白杨素(3)、野黄芩苷(4)、6-羟基木犀草素(5)、野黄芩素(6)、木犀草素(7)、异野黄芩素(8)、芹菜素(9)。大孔树脂50%乙醇洗脱部分抗乳腺癌活性最好。化合物7对MDA-MB-231细胞和HCC38细胞株体外增殖的抑制作用最好,其次为化合物5,而化合物4、6、9则表现出较弱的抑制作用。同时,化合物3、8对MDA-MB-231细胞体外增殖有较弱的抑制作用。结论化合物1~3为首次从该植物中分离。化合物5、7可以作为乳腺癌药物研究的潜在分子。

基于高效液相色谱指纹图谱及化学计量学的半枝莲质量分析

目的建立半枝莲高效液相色谱(high performance liquid chromatography,HPLC)指纹图谱联用化学计量学的质量分析方法。方法用InertSustain C_(18)(250 mm×4.6 mm,5μm),乙腈-1 mL·L^(-1)磷酸溶液梯度洗脱,梯度流速,进样量为10μL,波长为335 nm,柱温为30℃,用相似度评价法、聚类分析、主成分分析(principle component analysis,PCA)和偏最小二乘法判别分析(partial least squares discriminant analysis,PLS-DA)评价。结果12批样品相似度均大于0.90;聚类分析河南为一类,云南为一类,广东、广西为一类;PCA分类结果与聚类分析结果一致,差异大的色谱峰有10个;PLS-DA分类结果与聚类分析结果一致,差异大的色谱峰有14个;筛选半枝莲潜在质量标志物的色谱峰有7个,为10、11(木犀草苷)、12(野黄芩苷)、14、16、18、19号峰。结论建立了半枝莲高效液相色谱指纹图谱联用化学计量学的质量分析方法,为半枝莲质量标准研究提供依据。

基于网络药理学研究半枝莲抗三阴性乳腺癌的作用机制

目的利用网络药理学探讨半枝莲治疗三阴性乳腺癌的作用机制。方法本研究采用网络药理学方法,主要包括靶点预测、网络构建和功能富集。从中药系统药理学数据库与分析平台中获取“半枝莲”所有化合物靶点,并与其活化物进行匹配,发现木犀草素和汉黄芩素为主要相关化合物。构建蛋白-蛋白相互作用网络获得相关靶点信息,构建半枝莲与三阴性乳腺癌相关靶点网络,分析半枝莲抗三阴性乳腺癌的关键信号通路。结果共获得15个活性化合物的104个靶点。网络分析得出10个半枝莲治疗三阴性乳腺癌的关键靶点。功能富集分析表明,半枝莲可能通过协同调节多种生物学相关信号通路,对三阴性乳腺癌产生治疗作用。结论半枝莲治疗三阴性乳腺癌的药理机制可能与其协同调节细胞凋亡、细胞周期、分化、增殖、迁移、侵袭和血管生成密切相关。

半枝莲二萜类化学成分及药理作用研究进展

半枝莲是一种传统抗癌中药,从中分离得到的二萜类化合物对人鼻咽癌、人口腔表皮样癌、人直肠癌等表现出较强的细胞毒活性,同时也具有较好的抗病毒、抗氧化等活性。本文对半枝莲中二萜类化合物,尤其是“同物异名”、“同名异物”进行了梳理,从化学成分、药理活性方面进行了综述,希望能对半枝莲中二萜类化合物的更一步深入研究提供理论基础。

半枝莲总黄酮提取工艺优化及抗氧化、抗肿瘤活性评价

本实验旨在优化半枝莲总黄酮提取工艺,评价半枝莲总黄酮纯化物抗氧化、抗肿瘤活性。采用单因素实验结合响应面Box-Behnken设计对半枝莲总黄酮提取工艺进行研究,主要考察了提取时间、料液比、提取温度和乙醇体积分数对黄酮得率的影响,从而得出半枝莲总黄酮提取的最佳工艺;采用DPPH、ABTS法检测半枝莲总黄酮纯化物的抗氧化活性;采用MTT法检测纯化物对NCI-H1299、HepG2、MHCC-97H、HuH-7细胞增殖的影响。结果表明,半枝莲总黄酮提取最佳工艺为提取时间93 min、料液比1:41(g/mL)、提取温度为68℃、乙醇体积分数为75%,该条件下半枝莲总黄酮得率为26.46 mg/g。抗氧化实验结果表明,半枝莲总黄酮纯化物对DPPH、ABTS+自由基有较好的清除作用,IC_(50)值分别为25.41、70.41μg/mL;抗肿瘤实验表明,在一定浓度范围内不同质量浓度的半枝莲总黄酮纯化物对肿瘤细胞增殖均有一定抑制作用,其NCI-H1299、HepG2、MHCC-97H、HuH-7细胞的IC_(50)值分别为168.6、330.5、269.2、335.8μg/mL。综上可知,该方法稳定可行、重复性好,能有效提取出半枝莲中总黄酮成分,且纯化后的半枝莲总黄酮具有良好的抗氧化及抗肿瘤活性,可以进行功能性食品的研发。