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不同磁珠对致病菌吸附性能的比较研究 被引量:3
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作者 李倩倩 陈萍 任常菲 《卫生研究》 CAS CSCD 北大核心 2012年第2期293-297,共5页
目的通过对裸磁珠、单一抗体和混合抗体包被的免疫磁珠的吸附性能的比较,建立一种快速、同时富集多种致病菌的方法。方法通过沉淀法制备Fe3O4磁珠,对其进行表面修饰,用抗体进行偶联制备成免疫磁珠,比较不同磁珠对致病菌的吸附性能。结... 目的通过对裸磁珠、单一抗体和混合抗体包被的免疫磁珠的吸附性能的比较,建立一种快速、同时富集多种致病菌的方法。方法通过沉淀法制备Fe3O4磁珠,对其进行表面修饰,用抗体进行偶联制备成免疫磁珠,比较不同磁珠对致病菌的吸附性能。结果通过化学合成和修饰的方法制备出裸磁珠和Fe3O4/SiO2的免疫磁珠。免疫磁珠对细菌的吸附率与裸磁珠比较差异显著(t=7.55,P<0.05),平均吸附率分别为96.62%和80.89%。通过洗涤优化,免疫磁珠对目标菌的吸附率从97.03%降到93.67%,对非目标菌的吸附率从53.33%降到16.67%;裸磁珠对目标菌和非目标菌的吸附率从81%降到13.33%;免疫磁珠对单一菌和混合菌的吸附性能无显著差异(P=0.427)。结论单一抗体和混合抗体包被的免疫磁珠能特异性富集目标菌,通过富集程序优化可以达到良好的吸附效果。 展开更多
关键词 裸磁珠 免疫磁珠 吸附性能 食源性致病菌
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Role of a Single Shield in Thermocouple Measurements in Hot Air Flow 被引量:2
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作者 ma hongwei shi lei tian yangtao 《Journal of Thermal Science》 SCIE EI CAS CSCD 2017年第6期523-532,共10页
To investigate the role of a single shield on steady temperature measurement using therrnocouples in hot air flow, a methodology for solving convection, conduction, and radiation in one single model is provided. In or... To investigate the role of a single shield on steady temperature measurement using therrnocouples in hot air flow, a methodology for solving convection, conduction, and radiation in one single model is provided. In order to compare with the experimental results, a cylindrical computational domain is established, which is the same size with the hot calibration wind-tannel. In the computational domain, two kinds of thermocouples, the bare-bead and the single-shielded thermocouples, are simulated respectively. Surface temperature distribution and the tempera- ture measurement bias of the two typical thermocouples are compared. The simulation results indicate that: 1) The existence of the shield reduces bead surface heat flux and changes the direction of wires inner heat conduction in a colder surrounding; 2) The existence of the shield reduces the temperature measurement bias both by improving bead surface temperature and by reducing surface temperature gradient; 3) The shield effectively reduces the effect of the ambient temperature on the temperature measurement bias; 4) The shield effectively reduces the influence of airflow velocity on the temperature measurement bias. 展开更多
关键词 single-shield bare-bead thermocouples temperature measurement fluid-solid conjugated heat transfer.
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