A novel continuous ovary cell line from barfin flounder(Verasper moseri)(BFO cell line) was established with its primitive application in transgenic expression demonstrated in this study. Primarily cultured cells grew...A novel continuous ovary cell line from barfin flounder(Verasper moseri)(BFO cell line) was established with its primitive application in transgenic expression demonstrated in this study. Primarily cultured cells grew well at 22℃ in Dulbecco's modified Eagle medium/F12 medium(DMEM/F12, 1:1; p H 7.2) supplemented with 20% fetal bovine serum(FBS), carboxymethyl chitooligosaccharide, basic fibroblast growth factor(b FGF) and insulin-like growth factor-I(IGF-I). The primary BFO cells in fibroblastic morphology proliferated into a confluent monolayer about 2 weeks later, and were able to be subcultured. Impacts of medium and temperature on the growth of the cells were examined. The optimum growth was found in DMEM/F12 with 20% FBS and at 22℃. The BFO cells can be continuously subcultured to Passage 120 steadily with a population doubling time of 32.7 h at Passage 60. Chromosome analysis revealed that 72% of BFO cells at Passage 60 maintained the normal diploid chromosome number(46) with a normal karyotype of 2st+44t. The results of gene transformation indicated that green fluorescence protein(GFP) positively expressed in these cells after being transformed with pc DNA3.1-GFP. Therefore, a continuous and transformable BFO cell line was successfully established, which may serve as a useful tool for cytotechnological manipulation and transgenic modification of this fish.展开更多
The genetic structure and variation of the artificial meio- gynogenetic population and common population of barfin flounder （Verasper moseri） were analyzed using eight microsatellite markers. A total of 29 alleles w...The genetic structure and variation of the artificial meio- gynogenetic population and common population of barfin flounder （Verasper moseri） were analyzed using eight microsatellite markers. A total of 29 alleles were detected, of which 23 alleles were in the artificial gynogenetic population while 29 alleles were in the control group. The average observed heterozygosity （Ho） of eight loci in the control group （0.526 8） was several times higher than that （0.185 8） in the gynogenetic population. The results indicate that the genetic diversity of the control group was much higher than that of the gynogenetic population ofbarfin flounder （Verasper moseri）. Most loci significantly deviated from Hardy-Weinberg equilib- rium （HWE） after Bonferroni correction （p 〈 0.005 56） in the gynogenetic population, while four loci deviated from HWE in the control group. The coefficient of gene differentiation （GsT） was 0.131 0, and the genetic distance was 0.171 8 between the two populations, suggesting a significant genetic differentiation between the two populations.展开更多
应用石蜡切片技术和显微观察法，对从孵化后到变态完成（55～60日龄）的条斑星鲽（Verasper moseri Jordan et Gilbert）仔稚幼鱼早期发育中免疫系统的组织结构进行了研究，描述了个体发育过程中的组织学结构特征。实验表明；在13～15...应用石蜡切片技术和显微观察法，对从孵化后到变态完成（55～60日龄）的条斑星鲽（Verasper moseri Jordan et Gilbert）仔稚幼鱼早期发育中免疫系统的组织结构进行了研究，描述了个体发育过程中的组织学结构特征。实验表明；在13～15℃，条斑星鲽免疫器官原基出现的先后顺序为肾脏、脾脏和胸腺。孵化后即出现肾脏原基，8日龄和12日龄分别出现脾脏和胸腺原基；而免疫器官淋巴化的先后顺序较为特殊，首先淋巴化的器官是头肾，其次为胸腺和脾脏，不同于大多数海水鱼类。在其免疫器官发生后期除头肾和脾脏外，在胸腺中同样出现黑色素巨嗜细胞中心，虽然在数量和形态上不如头肾和脾脏丰富。作者通过对条王醺星鲽免疫器官发生过程研究，初步了解了其免疫器官发生，成熟过程以及特点，为进一步开展条斑星鲽的规模化养殖，以及人工疫苗的应用提供理论依据。展开更多
应用石蜡切片技术和显微测量法，对从孵化后到变态完成（55～60d，day post hatching，dph）的条斑星鲽（Verasper moseri Jordan et Gilbert）仔稚幼鱼早期发育中消化道的结构变化进行了观察，描述了个体发育过程中的组织学结构特征。...应用石蜡切片技术和显微测量法，对从孵化后到变态完成（55～60d，day post hatching，dph）的条斑星鲽（Verasper moseri Jordan et Gilbert）仔稚幼鱼早期发育中消化道的结构变化进行了观察，描述了个体发育过程中的组织学结构特征。结果表明，在13～15℃，7dph的仔鱼开口时，消化道已经分化为口咽腔、食道、肠和直肠，消化器官肝脏和胰腺出现。从7dph到14～17dph（卵黄囊消失）为混合营养期，形态上肠道进一步弯曲、盘旋；功能上中肠空泡样结构和后肠嗜伊红颗粒的出现，预示着肠上皮细胞在胃功能成熟前有吸收蛋白的作用。形态学上胃以及幽门盲囊的分化出现在26～29dph，与稚鱼刚开始附底的时期相吻合。而功能性胃的分化则出现在35dph左右，与稚鱼变态期相一致。条斑星鲽作为中国新近养殖品种，关于其早期发育的报道还是空白，作者对其消化系统进行系统的研究，旨在为该鱼种的商业化养殖提供相关的科学数据，同时也为海水鱼类早期营养和发育生物学的深入研究提供理论依据。展开更多
在人工养殖的条件下（自然光照，水温为11～16．3℃），对初始平均体质量为29．64g土5．98g的条斑星鲽（Verasper moseri Jordan et Gilbert）幼鱼1周年的生长和生态转化效率进行定期测定。结果显示：条斑星鲽的体长和体高在实验期间皆...在人工养殖的条件下（自然光照，水温为11～16．3℃），对初始平均体质量为29．64g土5．98g的条斑星鲽（Verasper moseri Jordan et Gilbert）幼鱼1周年的生长和生态转化效率进行定期测定。结果显示：条斑星鲽的体长和体高在实验期间皆呈直线增长；体质量的增长可以分为两个阶段：前期呈指数生长，后期为直线增长。体长和体质量呈指数函数关系：W=10^-5 L^3.0226，r=0．9893。条斑星鲽幼鱼周年的生长率和生态转化效率都较高，属于高生长类型鱼类；生态转化效率周年波动范围是34．16％～91．07％，特定生长率的周年波动范围是0．170～1．527．展开更多
基金supported by grants from the National 863 High Technology Research Foundation of China(2006AA10A401)the National Natural Science Foundation of China(31001100)
文摘A novel continuous ovary cell line from barfin flounder(Verasper moseri)(BFO cell line) was established with its primitive application in transgenic expression demonstrated in this study. Primarily cultured cells grew well at 22℃ in Dulbecco's modified Eagle medium/F12 medium(DMEM/F12, 1:1; p H 7.2) supplemented with 20% fetal bovine serum(FBS), carboxymethyl chitooligosaccharide, basic fibroblast growth factor(b FGF) and insulin-like growth factor-I(IGF-I). The primary BFO cells in fibroblastic morphology proliferated into a confluent monolayer about 2 weeks later, and were able to be subcultured. Impacts of medium and temperature on the growth of the cells were examined. The optimum growth was found in DMEM/F12 with 20% FBS and at 22℃. The BFO cells can be continuously subcultured to Passage 120 steadily with a population doubling time of 32.7 h at Passage 60. Chromosome analysis revealed that 72% of BFO cells at Passage 60 maintained the normal diploid chromosome number(46) with a normal karyotype of 2st+44t. The results of gene transformation indicated that green fluorescence protein(GFP) positively expressed in these cells after being transformed with pc DNA3.1-GFP. Therefore, a continuous and transformable BFO cell line was successfully established, which may serve as a useful tool for cytotechnological manipulation and transgenic modification of this fish.
基金Supported by the National High Technology Research and Devel-opment Program of China (863 Program) (2006AA10A403)Shandong Genetic Im-provement Key Project for Agricultural OrganismTaishan Scholar Project of Shan-dong Province
文摘The genetic structure and variation of the artificial meio- gynogenetic population and common population of barfin flounder （Verasper moseri） were analyzed using eight microsatellite markers. A total of 29 alleles were detected, of which 23 alleles were in the artificial gynogenetic population while 29 alleles were in the control group. The average observed heterozygosity （Ho） of eight loci in the control group （0.526 8） was several times higher than that （0.185 8） in the gynogenetic population. The results indicate that the genetic diversity of the control group was much higher than that of the gynogenetic population ofbarfin flounder （Verasper moseri）. Most loci significantly deviated from Hardy-Weinberg equilib- rium （HWE） after Bonferroni correction （p 〈 0.005 56） in the gynogenetic population, while four loci deviated from HWE in the control group. The coefficient of gene differentiation （GsT） was 0.131 0, and the genetic distance was 0.171 8 between the two populations, suggesting a significant genetic differentiation between the two populations.
文摘应用石蜡切片技术和显微观察法，对从孵化后到变态完成（55～60日龄）的条斑星鲽（Verasper moseri Jordan et Gilbert）仔稚幼鱼早期发育中免疫系统的组织结构进行了研究，描述了个体发育过程中的组织学结构特征。实验表明；在13～15℃，条斑星鲽免疫器官原基出现的先后顺序为肾脏、脾脏和胸腺。孵化后即出现肾脏原基，8日龄和12日龄分别出现脾脏和胸腺原基；而免疫器官淋巴化的先后顺序较为特殊，首先淋巴化的器官是头肾，其次为胸腺和脾脏，不同于大多数海水鱼类。在其免疫器官发生后期除头肾和脾脏外，在胸腺中同样出现黑色素巨嗜细胞中心，虽然在数量和形态上不如头肾和脾脏丰富。作者通过对条王醺星鲽免疫器官发生过程研究，初步了解了其免疫器官发生，成熟过程以及特点，为进一步开展条斑星鲽的规模化养殖，以及人工疫苗的应用提供理论依据。
文摘应用石蜡切片技术和显微测量法，对从孵化后到变态完成（55～60d，day post hatching，dph）的条斑星鲽（Verasper moseri Jordan et Gilbert）仔稚幼鱼早期发育中消化道的结构变化进行了观察，描述了个体发育过程中的组织学结构特征。结果表明，在13～15℃，7dph的仔鱼开口时，消化道已经分化为口咽腔、食道、肠和直肠，消化器官肝脏和胰腺出现。从7dph到14～17dph（卵黄囊消失）为混合营养期，形态上肠道进一步弯曲、盘旋；功能上中肠空泡样结构和后肠嗜伊红颗粒的出现，预示着肠上皮细胞在胃功能成熟前有吸收蛋白的作用。形态学上胃以及幽门盲囊的分化出现在26～29dph，与稚鱼刚开始附底的时期相吻合。而功能性胃的分化则出现在35dph左右，与稚鱼变态期相一致。条斑星鲽作为中国新近养殖品种，关于其早期发育的报道还是空白，作者对其消化系统进行系统的研究，旨在为该鱼种的商业化养殖提供相关的科学数据，同时也为海水鱼类早期营养和发育生物学的深入研究提供理论依据。
文摘在人工养殖的条件下（自然光照，水温为11～16．3℃），对初始平均体质量为29．64g土5．98g的条斑星鲽（Verasper moseri Jordan et Gilbert）幼鱼1周年的生长和生态转化效率进行定期测定。结果显示：条斑星鲽的体长和体高在实验期间皆呈直线增长；体质量的增长可以分为两个阶段：前期呈指数生长，后期为直线增长。体长和体质量呈指数函数关系：W=10^-5 L^3.0226，r=0．9893。条斑星鲽幼鱼周年的生长率和生态转化效率都较高，属于高生长类型鱼类；生态转化效率周年波动范围是34．16％～91．07％，特定生长率的周年波动范围是0．170～1．527．
