Background： Colibacillosis caused by enterotoxigenic Escherichia coil （E. coil} results in economic losses in the poultry industry. Antibiotics are usually used to control colibacillosis, however, E. coli has varyin...Background： Colibacillosis caused by enterotoxigenic Escherichia coil （E. coil} results in economic losses in the poultry industry. Antibiotics are usually used to control colibacillosis, however, E. coli has varying degrees of resistance to different antibiotics. Therefore the use of probiotics is becoming accepted as an alternative to antibiotics. In this study, we evaluated the effects of Clostfidium butyricum （C. butyficum） on growth performance, immune response, intestinal barrier function, and digestive enzyme activity in broiler chickens challenged with Eschefichia coli （E. coil） K88. Methods： The chickens were randomly divided into four treatment groups for 28 days. Negative control treatment （NC） consisted of birds fed a basal diet without E. coil K88 challenge and positive control treatment （PC） consisted of birds fed a basal diet and challenged with E. coil K88. C. buO/ricum probiotic treatment （CB） consisted of birds fed a diet containing 2 x 107 cfu C. buO/ricum/kg of diet and challenged with E. coil K88. Colistin sulfate antibiotic treatment （CS） consisted of birds fed a diet containing 20 mg colistin sulfate/kg of diet and challenged with E. coil K88. Results： The body weight （BW） and average day gain （ADG） in the broilers of CB group were higher （P 〈 0.05） than the broilers in the PC group overall except the ADG in the 14-21 d post-challenge. The birds in CB treatment had higher （P 〈 0.05） concentration of tumor necrosis factor-a （TNF-a） at 3 and 7 d post-challenge, and higher （P 〈 0.05） concentration of interleukin-4 （IL-4） at 14 d post-challenge than those in the PC treatment group. The concentration of serum endotoxin in CB birds was lower （P 〈 0.05） at 21 d post-challenge, and the concentrations of serum diamine oxidase in CB birds were lower （P 〈 0.05） at 14 and 21 d post-challenge than in PC birds. Birds in CB treatment group had higher （P 〈 0.05） jejunum villi height than those in PC, NC, or CS treatment at 7, 14, and 21 d post-challenge. In comparison to PC birds, the CB birds had lower （P 〈 0.05） jejunum crypt depth during the whole experiment. The birds in CB or CS treatment group had higher （P 〈 0.05） activities of amylase and protease at 3, 7, and 14 d post-challenge, and higher （P 〈 0.05） activity of lipase at 3, 7 d post-challenge than PC birds.展开更多
The numerical simulation for a new type of precast concrete barrier for viaducts is carried out systematically.To obtain an accurate representation of the damage state of the concrete barrier under the impact of a veh...The numerical simulation for a new type of precast concrete barrier for viaducts is carried out systematically.To obtain an accurate representation of the damage state of the concrete barrier under the impact of a vehicle,a stochastic damage-plasticity model of the concrete is adopted in the finite element model.Meanwhile,a simplified mathematical model of the impact between vehicles and the concrete barrier was established and the input energy was converted to the impact load to facilitate the investigation of the safety performance of the concrete barriers.On this basis,a refined finite element(FE)model of a precast concrete barrier was developed.The impact locations,impact load,boundary constraints,and reinforcement types were used as variables and the dynamic response of the precast concrete barrier was systematically analyzed under 36 working conditions.The simulated results indicated that the damage state of the concrete barrier and the stress of the reinforcing bars were accurately and quantitatively reflected.According to the computed results,some suggestions were put forward for selecting the appropriate reinforcement type of the precast concrete barrier and the connection between the superstructure and foundation.展开更多
Background Zinc glycine chelate(Zn-Gly)has anti-inflammation and growth-promoting properties;however,the mechanism of Zn-Gly contribution to gut barrier function in Cherry Valley ducks during intestinal inflammation i...Background Zinc glycine chelate(Zn-Gly)has anti-inflammation and growth-promoting properties;however,the mechanism of Zn-Gly contribution to gut barrier function in Cherry Valley ducks during intestinal inflammation is unknown.Three-hundred 1-day-old ducks were divided into 5 groups(6 replicates and 10 ducks per replicate)in a completely randomized design:the control and dextran sulfate sodium(DSS)groups were fed a corn-soybean meal basal diet,and experimental groups received supplements of 70,120 or 170 mg/kg Zn in form of Zn-Gly.The DSS and treatment groups were given 2 mL of 0.45 g/mL DSS daily during d 15–21,and the control group received normal saline.The experiment lasted 21 d.Results Compared with DSS group,70,120 and 170 mg/kg Zn significantly increased body weight(BW),villus height and the ratio of villus to crypt,and significantly decreased the crypt depth of jejunum at 21 d.The number of goblet cells in jejunal villi in the Zn-Gly group was significantly increased by periodic acid-Schiff staining.Compared with control,the content of intestinal permeability marker D-lactic acid(D-LA)and fluxes of fluorescein isothiocyanate(FITC-D)in plasma of DSS group significantly increased,and 170 mg/kg Zn supplementation significantly decreased the D-LA content and FITC-D fluxes.Compared with control,contents of plasma,jejunum endotoxin and jejunum pro-inflammatory factors IL-1β,IL-6 and TNF-αwere significantly increased in DSS group,and were significantly decreased by 170 mg/kg Zn supplementation.Dietary Zn significantly increased the contents of anti-inflammatory factors IL-10,IL-22 and sIgA and IgG in jejunum.Real-time PCR and Western blot results showed that 170 mg/kg Zn supplementation significantly increased mRNA expression levels of CLDN-1 and expression of OCLN protein in jejunum,and decreased gene and protein expression of CLDN-2 compared with DSS group.The 120 mg/kg Zn significantly promoted the expressions of IL-22 and IgA.Dietary Zn-Gly supplementation significantly decreased pro-inflammatory genes IL-8 and TNF-αexpression levels and TNF-αprotein expression in jejunum.Additionally,Zn significantly reduced the gene and protein expression of TLR4,MYD88 and NF-κB p65.Conclusions Zn-Gly improved duck BW and alleviated intestinal injury by regulating intestinal morphology,barrier function and gut inflammation-related signal pathways TLR4/MYD88/NF-κB p65.展开更多
Locomotor performance in lizards is strongly affected by structural habitat. Understanding this relationship allows us to predict species distributions across habitat types. However, little information is available ab...Locomotor performance in lizards is strongly affected by structural habitat. Understanding this relationship allows us to predict species distributions across habitat types. However, little information is available about the ecological role of the locomotion of multiocellated racerunner （Eremias multiocellata） in the desert steppe ecosystem of Inner Mongolia, China. Herein, we studied the effects of habitat structure on the locomotor performance of this lizard species in the field. We found that the sprint speed of this lizard declined significantly with increasing vegetation coverage. Manipulative experiments were further conducted to examine the effects of branch barriers and surface substrates on the sprint speed of the lizard. We found that the sprint speed was significantly influenced by the surface substrates and branch barriers, and there were no interactions between them. Branch barriers impeded sprint speed, and E. multiocellata showed better locomotor performance on sandy rather than loamy substrates. Our results indicate that E. multiocellata tends to occupy open areas with sandy substrates, but its locomotor performance is not closely associated with habitat preference.展开更多
Road side barriers are constructed to protect passengers and contain vehicles when a vehicle crashes into a barrier. In general, full-scale crash testing needs to be carried out if a geometrically and structurally equ...Road side barriers are constructed to protect passengers and contain vehicles when a vehicle crashes into a barrier. In general, full-scale crash testing needs to be carried out if a geometrically and structurally equivalent barrier has not previously been proven to meet the requirements of containing the vehicle and dissipating sufficient impact energy for passenger protection. As full-scale crash testing is very expensive, the number of data that can be measured in a test is usually limited, and it may not always be possible to obtain good quality measurements in such a test, a reliable and efficient numerical simulation of crash testing is therefore very useful. This paper presents finite element simulations of a 3-rail steel road traffic barrier under vehicle impact. The performance levels defined in Australian Standards AS5100 Clause 10.5 for these barriers are checked. The numerical simulations show that the barrier is able to meet low performance levels. However, the maximum deceleration is higher than the acceptable limit for passenger protection. If present, a kerb launches the vehicles into the barrier, allowing for the possibility of overriding the barrier under certain circumstances, but it redirects the vehicle and reduces the incident angle, which reduces impact force on the barrier. Further investigation into all common kerb profiles on roads should be carried out, as only one kerb profile is investigated in this study.展开更多
Front bumper,crash box and side rail are key body structural parts in front crash.Deformation space is affected by compartment packaging.The improvement suggestions are proposed to solve the problems existed in the cu...Front bumper,crash box and side rail are key body structural parts in front crash.Deformation space is affected by compartment packaging.The improvement suggestions are proposed to solve the problems existed in the current vehicle structure and compartment packaging based on the areas that influence performance of automobile offset deformable barrier impact, such as the side rail,mounting,storage battery packaging,etc.It is proved that 40%offset crash simulation result of one certain car is well-correlated with the physical test.Optimization cases meet the crash performance requirements.The objective of the analysis is to guide structural design and improves a car’s crash safety performance.展开更多
基金supported by the International Cooperation Project of Zhejiang Province(No.2012C14031)Innovative Research Team Program of Zhejiang Province(No.2011R50025)
文摘Background： Colibacillosis caused by enterotoxigenic Escherichia coil （E. coil} results in economic losses in the poultry industry. Antibiotics are usually used to control colibacillosis, however, E. coli has varying degrees of resistance to different antibiotics. Therefore the use of probiotics is becoming accepted as an alternative to antibiotics. In this study, we evaluated the effects of Clostfidium butyricum （C. butyficum） on growth performance, immune response, intestinal barrier function, and digestive enzyme activity in broiler chickens challenged with Eschefichia coli （E. coil） K88. Methods： The chickens were randomly divided into four treatment groups for 28 days. Negative control treatment （NC） consisted of birds fed a basal diet without E. coil K88 challenge and positive control treatment （PC） consisted of birds fed a basal diet and challenged with E. coil K88. C. buO/ricum probiotic treatment （CB） consisted of birds fed a diet containing 2 x 107 cfu C. buO/ricum/kg of diet and challenged with E. coil K88. Colistin sulfate antibiotic treatment （CS） consisted of birds fed a diet containing 20 mg colistin sulfate/kg of diet and challenged with E. coil K88. Results： The body weight （BW） and average day gain （ADG） in the broilers of CB group were higher （P 〈 0.05） than the broilers in the PC group overall except the ADG in the 14-21 d post-challenge. The birds in CB treatment had higher （P 〈 0.05） concentration of tumor necrosis factor-a （TNF-a） at 3 and 7 d post-challenge, and higher （P 〈 0.05） concentration of interleukin-4 （IL-4） at 14 d post-challenge than those in the PC treatment group. The concentration of serum endotoxin in CB birds was lower （P 〈 0.05） at 21 d post-challenge, and the concentrations of serum diamine oxidase in CB birds were lower （P 〈 0.05） at 14 and 21 d post-challenge than in PC birds. Birds in CB treatment group had higher （P 〈 0.05） jejunum villi height than those in PC, NC, or CS treatment at 7, 14, and 21 d post-challenge. In comparison to PC birds, the CB birds had lower （P 〈 0.05） jejunum crypt depth during the whole experiment. The birds in CB or CS treatment group had higher （P 〈 0.05） activities of amylase and protease at 3, 7, and 14 d post-challenge, and higher （P 〈 0.05） activity of lipase at 3, 7 d post-challenge than PC birds.
基金supported by the Green Science Architecture Industry Development Co.,Ltd.,Guizhou,551400,China.XL Gao received the grant,http://www.gzslzkj.com.
文摘The numerical simulation for a new type of precast concrete barrier for viaducts is carried out systematically.To obtain an accurate representation of the damage state of the concrete barrier under the impact of a vehicle,a stochastic damage-plasticity model of the concrete is adopted in the finite element model.Meanwhile,a simplified mathematical model of the impact between vehicles and the concrete barrier was established and the input energy was converted to the impact load to facilitate the investigation of the safety performance of the concrete barriers.On this basis,a refined finite element(FE)model of a precast concrete barrier was developed.The impact locations,impact load,boundary constraints,and reinforcement types were used as variables and the dynamic response of the precast concrete barrier was systematically analyzed under 36 working conditions.The simulated results indicated that the damage state of the concrete barrier and the stress of the reinforcing bars were accurately and quantitatively reflected.According to the computed results,some suggestions were put forward for selecting the appropriate reinforcement type of the precast concrete barrier and the connection between the superstructure and foundation.
基金supported by the Natural Science Foundation of Sichuan Province(No.2022NSFSC0060)。
文摘Background Zinc glycine chelate(Zn-Gly)has anti-inflammation and growth-promoting properties;however,the mechanism of Zn-Gly contribution to gut barrier function in Cherry Valley ducks during intestinal inflammation is unknown.Three-hundred 1-day-old ducks were divided into 5 groups(6 replicates and 10 ducks per replicate)in a completely randomized design:the control and dextran sulfate sodium(DSS)groups were fed a corn-soybean meal basal diet,and experimental groups received supplements of 70,120 or 170 mg/kg Zn in form of Zn-Gly.The DSS and treatment groups were given 2 mL of 0.45 g/mL DSS daily during d 15–21,and the control group received normal saline.The experiment lasted 21 d.Results Compared with DSS group,70,120 and 170 mg/kg Zn significantly increased body weight(BW),villus height and the ratio of villus to crypt,and significantly decreased the crypt depth of jejunum at 21 d.The number of goblet cells in jejunal villi in the Zn-Gly group was significantly increased by periodic acid-Schiff staining.Compared with control,the content of intestinal permeability marker D-lactic acid(D-LA)and fluxes of fluorescein isothiocyanate(FITC-D)in plasma of DSS group significantly increased,and 170 mg/kg Zn supplementation significantly decreased the D-LA content and FITC-D fluxes.Compared with control,contents of plasma,jejunum endotoxin and jejunum pro-inflammatory factors IL-1β,IL-6 and TNF-αwere significantly increased in DSS group,and were significantly decreased by 170 mg/kg Zn supplementation.Dietary Zn significantly increased the contents of anti-inflammatory factors IL-10,IL-22 and sIgA and IgG in jejunum.Real-time PCR and Western blot results showed that 170 mg/kg Zn supplementation significantly increased mRNA expression levels of CLDN-1 and expression of OCLN protein in jejunum,and decreased gene and protein expression of CLDN-2 compared with DSS group.The 120 mg/kg Zn significantly promoted the expressions of IL-22 and IgA.Dietary Zn-Gly supplementation significantly decreased pro-inflammatory genes IL-8 and TNF-αexpression levels and TNF-αprotein expression in jejunum.Additionally,Zn significantly reduced the gene and protein expression of TLR4,MYD88 and NF-κB p65.Conclusions Zn-Gly improved duck BW and alleviated intestinal injury by regulating intestinal morphology,barrier function and gut inflammation-related signal pathways TLR4/MYD88/NF-κB p65.
基金performed under the approval from the Animal Ethics Committee at the Institute of Zoology,Chinese Academy of Sciences(IOZ14001)
文摘Locomotor performance in lizards is strongly affected by structural habitat. Understanding this relationship allows us to predict species distributions across habitat types. However, little information is available about the ecological role of the locomotion of multiocellated racerunner （Eremias multiocellata） in the desert steppe ecosystem of Inner Mongolia, China. Herein, we studied the effects of habitat structure on the locomotor performance of this lizard species in the field. We found that the sprint speed of this lizard declined significantly with increasing vegetation coverage. Manipulative experiments were further conducted to examine the effects of branch barriers and surface substrates on the sprint speed of the lizard. We found that the sprint speed was significantly influenced by the surface substrates and branch barriers, and there were no interactions between them. Branch barriers impeded sprint speed, and E. multiocellata showed better locomotor performance on sandy rather than loamy substrates. Our results indicate that E. multiocellata tends to occupy open areas with sandy substrates, but its locomotor performance is not closely associated with habitat preference.
文摘Road side barriers are constructed to protect passengers and contain vehicles when a vehicle crashes into a barrier. In general, full-scale crash testing needs to be carried out if a geometrically and structurally equivalent barrier has not previously been proven to meet the requirements of containing the vehicle and dissipating sufficient impact energy for passenger protection. As full-scale crash testing is very expensive, the number of data that can be measured in a test is usually limited, and it may not always be possible to obtain good quality measurements in such a test, a reliable and efficient numerical simulation of crash testing is therefore very useful. This paper presents finite element simulations of a 3-rail steel road traffic barrier under vehicle impact. The performance levels defined in Australian Standards AS5100 Clause 10.5 for these barriers are checked. The numerical simulations show that the barrier is able to meet low performance levels. However, the maximum deceleration is higher than the acceptable limit for passenger protection. If present, a kerb launches the vehicles into the barrier, allowing for the possibility of overriding the barrier under certain circumstances, but it redirects the vehicle and reduces the incident angle, which reduces impact force on the barrier. Further investigation into all common kerb profiles on roads should be carried out, as only one kerb profile is investigated in this study.
基金"Twelfth Five-year Plan"for Sci & Tech Research of China(No.2011BAG03B02No.2011BAG03B06)
文摘Front bumper,crash box and side rail are key body structural parts in front crash.Deformation space is affected by compartment packaging.The improvement suggestions are proposed to solve the problems existed in the current vehicle structure and compartment packaging based on the areas that influence performance of automobile offset deformable barrier impact, such as the side rail,mounting,storage battery packaging,etc.It is proved that 40%offset crash simulation result of one certain car is well-correlated with the physical test.Optimization cases meet the crash performance requirements.The objective of the analysis is to guide structural design and improves a car’s crash safety performance.
