Establishment and characterization of a canine chondrosarcoma cell line:Mango

In the global progress of bone tumor research,established stable and long-lasting transgenic chondrosarcoma(CSA)cell lines are rare,mainly of murine and human origin,while the establishment of canine CSA cell lines has yet to be reported.This study established a canine CSA cell line to facilitate the basic clinical study of canine CSA.Fifty fve cases of canine osteolytic disease were collected,and more than 10 bone tumor samples from dogs with typical clinical signs were used for primary cell culture.A cell line with stable passaging for more than 100 generations and mouse tumorigenic ability was successfully cultured.According to the clinical characteristics of the dog and the histopathological results of the primary tumor,CSA was diagnosed,and the CSA cell line was designated Mango.Immunohistochemical(IHC)results showed that the immunoreactivity of bone gamma-carboxyglutamate protein(BGLAP),secreted protein acidic and rich in cysteine(SPARC),alkaline phosphatase(ALPL),vimentin(VIM)and S100 were positive.However,the immunoreactivity of pan-cytokeratin(PCK),chromogranin A(CGA),and platelet endothelial cell adhesion molecule-1(CD31)was negative.Immunofuorescence(IF)results showed that the protein expressions in the Mango cell line were consistent with the IHC identifcation of the primary tumor.The Mango cell line’s doubling time was 43.92 h,and the cell formation rate exceeded 20%.There were abnormal chromosome numbers,hetero staining with toluidine blue,and certain calcifcation abilities.It could be passaged stably and continuously without changing the cell morphology and characteristics.In vivo,the cells were successfully injected into the nude mice model with a tumorigenic rate of 100%.The immunophenotype of the xenograft tumor was consistent with that of the primary tumor.Therefore,we efectively established a canine CSA cell line.As a promising cell material,this cell line can be used to construct a tumor-bearing model conducive to the subsequent basic research of canine CSA.Moreover,because of its similarity to human CSA,the animal model of CSA is also indispensable for investigating human CSA.

New construction of mutually unbiased bases for odd-dimensional state space

We study the construction of mutually unbiased bases in Hilbert space for composite dimensions d which are not prime powers.We explore the results for composite dimensions which are true for prime power dimensions.We then provide a method for selecting mutually unbiased vectors from the eigenvectors of generalized Pauli matrices to construct mutually unbiased bases.In particular,we present four mutually unbiased bases in C^(15).

Principle for the Installation of Accommodation for Sick guards in Public Health Establishments in Brazzaville in the Republic of Congo

The results of scientific studies of human social facts in the field of health show that the management of a patient should involve the patient’s entourage,whatever the status or size of the health establishment.In healthcare establishments in the Congo,the following are recognised as being responsible for medical care:specialist doctors,doctors,midwives,nurses and care assistants.The patient’s family and close friends are responsible for looking after the patient and financing care.The hospital infrastructure does not provide any space for the patient warden who accompany the patient during reception and hospitalisation.This makes Congolese hospitals inefficient for patient care.How can we integrate the function of the Sick guard and the assistance of the family,in order to reduce the mortality rate and repair the harm caused to patients requiring the presence of relatives during their stay in hospital,which is considered to be a dangerous place?This article examines the functional principles for configuring the space that patient warden would occupy in the patient care system.On the basis of a documentary analysis of sociological and architectural studies of existing facilities,this article proposes a typical accommodation model with the spaces needed to ensure the well-being and effectiveness of the patient warden with the patient.These are rooms with minimum space for 2 to 4 individual beds,equipped with toilets and showers.The accommodation has a dining area,kitchen and laundry facilities.In the future,this accommodation will become part of the hospital estate and may be occupied by orderlies and patient warden recruited by the hospital administration.

Research on the Construction and Development of Aesthetic Education Bases in Higher Education Institutions

In order to implement the national aesthetic education policy,it is essential to organically integrate relevant aesthetic education resources,promote interdisciplinary education,and carry out extracurricular practical activities centered on aesthetic education.Strengthening the foundation of aesthetic education theory,addressing practical challenges in the teaching process,and closely linking research with teaching will create a feedback loop that benefits drama education.This article provides an in-depth analysis of the construction strategies,resource advantages and disadvantages,and development of aesthetic education bases,offering countermeasures and suggestions for their construction in universities.It aims to provide a solid research foundation for the growth and development of aesthetic education bases in higher education institutions.

Homogenization Construction of Three-Level Teaching Bases for General Practice Medicine Under the Internet+Model

With the continuous expansion of medical student enrollment,the number of clinical teaching bases is gradually increasing.However,there are significant differences in clinical teaching management models and teaching levels among different bases.Most clinical teaching bases have incomplete teaching management systems,inadequate teaching management institutions,insufficient teaching personnel,and inadequate implementation of teaching rules and regulations.This article combines the construction practice of three-level clinical teaching base of the General Medicine College and the First Affiliated Hospital of Xi’an Medical University.We establish a standardized management system for the three-level clinical teaching base;implement a teaching supervision system and strengthen the monitoring of teaching quality;adopt multiple evaluations to test the effectiveness of clinical teaching implementation;explore the path of homogenization construction of teaching bases in terms of unified teacher training,promoting the development of teacher teaching abilities with equal quality and excellence,and providing a reference for improving the quality of medical talent training.

First report on establishment and characterization of the extrahepatic cholangiocarcinoma sarcoma cell line CBC2T-2

BACKGROUND Extrahepatic cholangiocarcinoma sarcoma is extremely rare in clinical practice.These cells consist of both epithelial and mesenchymal cells.Patient-derived cell lines that maintain tumor characteristics are valuable tools for studying the molecular mechanisms associated with carcinosarcoma.However,cholangiocarcinoma sarcoma cell lines are not available in cell banks.AIM To establish and characterize a new extrahepatic cholangiocarcinoma sarcoma cell line,namely CBC2T-2.METHODS We conducted a short tandem repeat(STR)test to confirm the identity of the CBC2T-2 cell line.Furthermore,we assessed the migratory and invasive properties of the cells and performed clonogenicity assay to evaluate the ability of individual cells to form colonies.The tumorigenic potential of CBC2T-2 cells was tested in vivo using nonobese diabetic/severe combined immunodeficient(NOD/SCID)mice.The cells were injected subcutaneously and tumor formation was observed.In addition,immunohistochemical analysis was carried out to examine the expression of epithelial marker CK19 and mesenchymal marker vimentin in both CBC2T-2 cells and xenografts.The CBC2T-2 cell line was used to screen the potential therapeutic effects of various clinical agents in patients with cholangiocarcinoma sarcoma.Lastly,whole-exome sequencing was performed to identify genetic alterations and screen for somatic mutations in the CBC2T-2 cell line.RESULTS The STR test showed that there was no cross-contamination and the results were identical to those of the original tissue.The cells showed round or oval-shaped epithelioid cells and mesenchymal cells with spindle-shaped or elongated morphology.The cells exhibited a high proliferation ratio with a doubling time of 47.11 h.This cell line has migratory,invasive,and clonogenic abilities.The chromosomes in the CBC2T-2 cells were polyploidy,with numbers ranging from 69 to 79.The subcutaneous tumorigenic assay confirmed the in vivo tumorigenic ability of CBC2T-2 cells in NOD/SCID mice.CBC2T-2 cells and xenografts were positive for both the epithelial marker,CK19,and the mesenchymal marker,vimentin.These results suggest that CBC2T-2 cells may have both epithelial and mesenchymal characteristics.The cells were also used to screen clinical agents in patients with cholangiocarcinoma sarcoma,and a combination of paclitaxel and gemcitabine was found to be the most effective treatment option.CONCLUSION We established the first human cholangiocarcinoma sarcoma cell line,CBC2T-2,with stable biogenetic traits.This cell line,as a research model,has a high clinical value and would facilitate the understanding of the pathogenesis of cholangiocarcinoma sarcoma.

Physiological and molecular bases of the boron deficiency response in tomatoes

Boron is an essential microelement for plant growth.Tomato is one of the most cultivated fruits and vegetables in the world,and boron deficiency severely inhibits its yield and quality.However,the mechanism of tomato in response to boron deficiency remains largely unclear.Here,we investigated the physiological and molecular bases of the boron deficiency response in hydroponically grown tomato seedlings.Boron deficiency repressed the expression of genes associated with nitrogen metabolism,while it induced the expression of genes related to the pentose phosphate pathway,thereby altering carbon flow to provide energy for plants to cope with stress.Boron deficiency increased the accumulation of copper,manganese and iron,thereby maintaining chlorophyll content and photosynthetic efficiency at the early stage of stress.In addition,boron deficiency downregulated the expression of genes involved in cell wall organization and reduced the contents of pectin and cellulose in roots,ultimately retarding root growth.Furthermore,boron deficiency markedly altered phytohormone levels and signaling pathways in roots.The contents of jasmonic acid,jasmonoy1-L-isoleucine,trans-zeatin riboside,abscisic acid,salicylic acid,and SA glucoside were decreased;in contrast,the contents of isopentenyladenine riboside and ethylene precursor 1-aminocyclopropane-1-carboxylic acid were increased in the roots of boron-deficient tomato plants.These results collectively indicate that tomato roots reprogram carbon/nitrogen metabolism,alter cell wall components and modulate phytohormone pathways to survive boron deficiency.This study provides a theoretical basis for further elucidating the adaptive mechanism of tomato in response to boron deficiency.

Synthesis, Characterization and Biological Activity Evaluation of Schiff Bases Derived from 1,8-Diaminonaphtalène

The compounds have been synthesized and characterized by routine MS, IR and NMR spectrometry methods. The compounds are all active on bacterial strains with the exception of Salmonella typhimirium, with a MIC value of 7.5 mg/mL. They show a percentage of anti-radical activity of 75.476 ± 5.070 for the compound DAN-S and of 68.142 ± 6.539 for the compound DAN-OV. The compounds are sensitive to the two champions used. DAN-S compound is then the most active.

Solving the k-Independent Sets Problem of Graphs by Gröbner Bases

The aim of this paper is to given an algebraic computational method for finding maximal independent sets as well as the independent number of an arbitrary finite graph of n vertices G by strengthening the problem of finding maximal independent sets of G to the problem of finding k-independent sets in G for. It is shown that the existence of k-independent sets in G is equivalent to the existence of solutions of a system of multivariate polynomial equations. It follows that the problem of finding k-independent sets can be realized by using Gröbner bases of polynomial ideals. Since the number of k-independent sets is finite, the triangular equations composed by Gröbner bases are easier to be solved. Consequently, the maximal independent sets and the independent number of G are obtained after solving at most n such equations. Finally, the numerical example is presented to illustrate the effectiveness of this algebraic computational method.

Novel Mannich Bases of Benzimidazole Derivatives: An Antibacterial Study of Environmental Bacterial Strains

A previous study was conducted on the synthesis and antibacterial evaluation of Mannich bases of 2-(thioalkyl)-1H-methylbenzimidazole derivatives. The results of this study showed that certain 2-(thioalkyl)-1H-methylbenzimidazole and 2-(thioalkyl)-methyl-1-(piperidin-1-ylmethyl)benzimidazole derivatives possess antibacterial activities against clinical strains, such as Escherichia coli, Klebsiella pneumonia (Gram-negative bacteria), Staphylococcus aureus and Pseudomonas aeruginosa (Gram-positive bacteria). Following these favorable results, we extended the antibacterial evaluation of this series of molecules to environmental strains. The aim of this study was to assess the impact of the methyl-piperidine group fixed at position-1 of this new series of benzimidazoles on the antibacterial activity of environmental strains. In addition, we first evaluated the antibacterial activity of four 2-(thioalkyl)methylbenzimidazole derivatives and then that of five 2-(thioalkyl)-methyl-1-(piperidin-1-ylmethyl) benzimidazole derivatives. This study allowed us to show that compounds 1d and 1e could inhibit bacterial growth in vitro of the Enterobacteria P1 strain with inhibition diameters of 17.3 ± 0.6 mm and 10 ± 0.0 mm, respectively. However, addition of methyl-piperidinyl in this series showed that five (5) of 2-(thioalkyl)-methyl-1-(piperidin-1-ylmethyl) benzimidazole derivatives had an inhibitory effect on the in vitro growth of bacterial strains used except on Enterobacteria P2 with inhibition diameters between 10.0 ± 0.8 mm and 27.9 ± 1.4 mm. The introduction of the methyl-piperidinyl group at the 1-position of 2-(thioalkyl)-1H-methylbenzimidazole derivatives greatly improved the antibacterial activity against environmental bacteria such as Escherichia coli A1, A2, A3, and A4 and two Enterobacteria P1.

Discussion on Construction and Mechanism of Practice Bases for Professional Degree Postgraduates Based on the Integration of Production and Education in Local Universities

Under the background of emerging agricultural education,practice bases are an important place to train profession talented person that match social needs,so it is very important to build stable and long-term practice bases.At present,the main problems in the construction of practice bases for postgraduates in local colleges and universities are mainly focusing on construction and neglecting management,imperfect cooperation mechanism and lack of teaching management effectively.It is of great significance to build a long-term management mechanism to ensure the education quality of practice bases.Therefore,constructing the mode of"double integration and double promotion of production and education"with practice bases as the carrier and realizing the organic combination of industrial needs and postgraduate projects is an important idea for local colleges and universities to train professional degree postgraduates to"know agriculture,love agriculture,strengthen agriculture and promote agriculture",and it also provides a reference for the mechanism construction of postgraduate bases.

我国高校图书馆在编馆员减少的原因分析与应对策略

文章运用问卷调研法和网络调研法,分析高校图书馆在编馆员减少的现状及其成因,指出由此引发的工作压力大、人员管理困难、图书馆话语权减弱、馆员队伍稳定性不足等问题,并提出高校图书馆的应对策略,即开展系统研究,注重科学决策;明确职业定位,重塑职业精神;提升管理能力,整合人力资源;突出工作业绩,寻求学校支持;挖掘馆员潜力,利用外部资源;严格人事考核,紧抓工作成效;制定规划,保障队伍持续发展。

新工科背景下电子信息类专业课教学改革与实践

基于OBE理念,对电子信息类专业课进行了“1中心+3融合+5驱动”的教学改革实践,搭建“四方联动”的教学环境,设计“全方位”考评机制,落实立德树人根本任务和“两性一度”培养目标,为新工科背景下的电子信息类专业创新型人才培养奠定基础。

多智能体深度强化学习研究进展

深度强化学习(Deep Reinforcement Learning,DRL)在近年受到广泛的关注,并在各种领域取得显著的成功.由于现实环境通常包括多个与环境交互的智能体,多智能体深度强化学习(Multi-Agent Deep Reinforcement Learning,MADRL)获得蓬勃的发展,在各种复杂的序列决策任务上取得优异的表现.本文对多智能体深度强化学习的工作进展进行综述,主要内容分为三个部分.首先,我们回顾了几种常见的多智能体强化学习问题表示及其对应的合作、竞争和混合任务.其次,我们对目前的MADRL方法进行了全新的多维度的分类,并对不同类别的方法展开进一步介绍.其中,我们重点综述值函数分解方法,基于通信的MADRL方法以及基于图神经网络的MADRL方法.最后,我们研究了MADRL方法在现实场景中的主要应用.希望本文能够为即将进入这一快速发展领域的新研究人员和希望获得全方位了解并根据最新进展确定新方向的现有领域专家提供帮助.

立德树人视阈下高校武术课程思政实践路径研究

立德树人是高校教育的根本任务,武术课程作为高校教育的重要组成部分,推进武术课程思政改革,完成立德树人根本任务,已成为当前高校体育教育的研究热点。通过文献资料法、比较分析法及文化学视角对立德树人的内涵进行分析,理清立德树人与高校武术课程思政的逻辑关系,提出通过挖掘和提炼武术德育因子,融课程思政于武术教育过程立德。研究认为:通过武术教学、武术课外锻炼与训练、武术竞赛等实践活动能够培育人才;通过培养德才兼备的人完成立德树人根本任务,达到高校武术课程思政的教育目的。

产融结合的同群效应研究——来自A股上市公司的经验证据

已有关于产融结合的研究较少从同群效应视角考察。本文基于中国沪、深两市A股2008—2020年非平衡面板数据,重点探究产融结合同群效应的存在合理性、产生机制及可能造成的经济后果。研究发现,产融结合分别在深度和广度两方面存在显著的同群效应,采用同群企业股票特质收益率作为工具变量及多项稳健性检验,结果依然成立。领导企业的产融结合同群效应表现为“竞争动机”,追随企业的产融结合同群效应是出于“信息动机”。产融结合同群效应能够降低企业面临的风险,但是会提高社会层面的整体风险水平。本研究拓展了同群效应及产融结合影响因素的研究边界,为政府部门完善有关经济政策、企业制定产融结合决策提供了理论依据与经验证据。

生物质碳气凝胶的研究现状及发展趋势

生物质碳气凝胶具有高孔隙率､大比表面积､突出的机械性能和非凡的导电性等优点,是全球绿色发展背景下的研究热点。对生物质碳气凝胶的发展历程､原料种类､制备过程､应用领域及未来发展前景等方面进行了综述。

CBL联合PBL教学法在心脏病介入诊治医师培训中的应用价值

目的探讨案例教学法(CBL)联合以问题为基础的教学法(PBL)在心脏病介入诊治医师培训中的应用价值。方法选取2020年3月至2022年3月西安交通大学第一附属医院介入培训基地的60名进修学员,按照教学方法不同将其分为LBL组和CBL+PBL组,各30名。LBL组采用传统的教学模式,CBL+PBL组采用CBL联合PBL的教学模式。比较两组的教学方法认同率、理论考核成绩、介入操作考核成绩。结果CBL+PBL组对于提高学习兴趣、培养临床思维能力、促进介入操作应用和记忆、提高分析和解决问题的能力、培养团队精神的认同率均显著高于LBL组(P<0.05)。CBL+PBL组的基础知识、问诊与查体、辅助检查分析能力、病例分析能力评分及总分均高于LBL组(P<0.05)。CBL+PBL组的血管穿刺及压迫、DSA机器操作、介入诊疗操作、术后观察及并发症处理评分及总分均高于LBL组(P<0.01)。结论CBL+PBL教学法在心脏病介入培训中能更好地调动学员的主观能动性,进而达到显著的教学效果。

煤电化基地大宗固废“三化”协同利用基础与技术

我国14个大型煤炭基地及89个大宗固体废弃物综合利用示范基地建设,标志着矿区大宗固废利用已被纳入全国战略发展布局。长期以来煤炭资源高强度的开发与利用,造成矿区浅埋煤层资源临近枯竭,煤电化基地大规模固废堆积及地表沉陷,已成为制约矿区绿色低碳、高质量发展的难题。大宗煤基固废协同利用与绿色充填是解放“三下一上”压煤,延长矿井服务年限,实现固废无害化、资源化、规模化“三化”利用的有效途径。基于产煤大省山西省、“华东能源粮仓”安徽两淮基地及宁东能源化工基地的煤基固废种类和产量,详细阐述了以煤矸石、粉煤灰、炉底渣、气化渣和脱硫石膏等为主要材料的煤基固废通过重金属吸附解吸和络合钝化技术实现无害化处置,列举煤基固废分类应用于低热值煤基固废发电、制备建筑材料如水泥、砖瓦等资源化利用途径,对比分析煤基固废采煤沉陷区复垦回填及井下充填规模化利用途径,突出煤基固废井下充填的优越性。基于煤电化基地深部煤炭资源,提出绿色充填开采理论与关键技术,包括深部煤矸石源头减量与采选充协同技术、充填材料高效制备与深部井下输送技术及煤基固废充填材料深部多场耦合机理,探究多源煤基固废从源头、过程到终端的深部充填开采全过程的技术原理与方法,以解决矿区深部井下充填的技术难题。根据宁东基地任家庄煤矿、山西省霍尔辛赫煤矿及淮北矿区地质条件和充填目的,分别提出超前钻孔注充低位充填方案、关键层非典型特征条件下多离层梯级注浆方案和煤基固废协同利用关键技术,综合矿山固废处置与利用、深部煤炭资源开发利用、地表沉陷控制、生态环境保护等优势,形成煤电化基地大宗固废协同利用与绿色开采模式,为煤炭开采高质量化和环境低损伤化提供参考。

内镜下经鼻或经口入路颅底脊索瘤切除术后生活质量与临床和影像学特征的相关分析

目的探讨内镜下经鼻或经口入路颅底脊索瘤切除术后生活质量与临床和影像学特征的相关性。方法回顾总结2016年3月至2022年3月在天津市环湖医院行内镜下经鼻或经口入路手术的14例颅底脊索瘤患者的临床和影像学特点,采用36条简明健康状况调查表(SF‑36)评价手术后生活质量,采用Point‑Biserial相关分析和偏相关分析探究SF‑36评分及其各维度评分与临床和影像学特征的相关性。结果相关分析显示,SF‑36评分中躯体健康总分之躯体疼痛维度评分与头痛(r=‑0.998,P=0.043)、复视(r=‑0.999,P=0.032)、视力障碍(r=‑0.998,P=0.037)、海绵窦症状(r=‑0.997,P=0.047)、肿瘤次全切除或大部切除(r=‑0.998,P=0.038)呈负相关,与术后放射治疗(r=0.998,P=0.038)、全斜坡型肿瘤(r=0.998,P=0.036)、肿瘤体积>20 cm3(r=0.999,P=0.035)呈正相关。结论脊索瘤患者可存在术前头痛、复视、视力障碍、海绵窦症状以及术中肿瘤次全切除或大部切除的特征,可降低内镜下经鼻或经口入路手术后生活质量,临床对此类患者应予以关注。