The sonic hedgehog protein not only plays a key role in early embryonic development, but also has essential effects on the adult nervous system, including neural stem cell proliferation, differentiation migration and ...The sonic hedgehog protein not only plays a key role in early embryonic development, but also has essential effects on the adult nervous system, including neural stem cell proliferation, differentiation migration and neuronal axon guidance. The N-terminal fragment of sonic hedgehog is the key functional element in this process. Therefore, this study aimed to clone and analyze the N-terminal fragment of the sonic hedgehog gene. Total RNA was extracted from the notochord of a Sprague-Dawley rat at embryonic day 9 and the N-terminal fragment of sonic hedgehog was amplified by nested reverse transcription-PCR. The N-terminal fragment of the sonic hedgehog gene was successfully cloned. The secondary and tertiary structures of the N-terminal fragment of the sonic hedgehog protein were predicted using Jpred and Phyre online.展开更多
In this paper, by the transparent-component-decimation （TCD） method we obtain three kinds of new basic- components （BCs） through simplifying and decomposing the BCs of three-component Thue-Morse （3CTM） sequence....In this paper, by the transparent-component-decimation （TCD） method we obtain three kinds of new basic- components （BCs） through simplifying and decomposing the BCs of three-component Thue-Morse （3CTM） sequence. Based on these new BCs we propose a type of basic-structural-units （BSUs） and investigate the optical transmission of the one-dimensional （1D） superlattices composed of these BSUs. It is found that if the substrates of the 1D BSU superlattices are certain, the optical transmission at the central wavelength （CW） will be determined completely by the number and the type of BSUs and has nothing to do with the marshalling sequence. In particular, if the substrates are identical, the numbers of different types of BSUs are all the same and the middle two elements of BSUs constitute a cycle, then no matter whether the system is periodic, or quasiperiodic, or aperiodic, or unordered, or even random, it will be transparent at the CW. The conclusion is confirmed by the numerical results. Similar to the even layers of neighbourhood identical elements in TCD method, such a kind of optical BSU subsystem can also be decimated from the chain in the process of transmission investigation. There would be a potential application in the designing of some interesting optical devices.展开更多
基金sponsored by the Guangdong Provincial Natural Science Foundation,No.S2012010009592the Science and Technology Talent Foundation of Guangdong Provincial Natural Science Foundation,No.30900725+2 种基金the Joint Research Program by Southern Medical University-Shunde Guizhou Hospital,No.09000608the Science Foshan Municipal Key Project in Medical Sciences,No.201008063and the Shunde Medical Research Program,No.2011050
文摘The sonic hedgehog protein not only plays a key role in early embryonic development, but also has essential effects on the adult nervous system, including neural stem cell proliferation, differentiation migration and neuronal axon guidance. The N-terminal fragment of sonic hedgehog is the key functional element in this process. Therefore, this study aimed to clone and analyze the N-terminal fragment of the sonic hedgehog gene. Total RNA was extracted from the notochord of a Sprague-Dawley rat at embryonic day 9 and the N-terminal fragment of sonic hedgehog was amplified by nested reverse transcription-PCR. The N-terminal fragment of the sonic hedgehog gene was successfully cloned. The secondary and tertiary structures of the N-terminal fragment of the sonic hedgehog protein were predicted using Jpred and Phyre online.
基金supported by the National Natural Science Foundation of China (Grant No.10974061)the Program for Innovative Research Team of the Higher Education in Guangdong,China (Grant No.06CXTD005)
文摘In this paper, by the transparent-component-decimation （TCD） method we obtain three kinds of new basic- components （BCs） through simplifying and decomposing the BCs of three-component Thue-Morse （3CTM） sequence. Based on these new BCs we propose a type of basic-structural-units （BSUs） and investigate the optical transmission of the one-dimensional （1D） superlattices composed of these BSUs. It is found that if the substrates of the 1D BSU superlattices are certain, the optical transmission at the central wavelength （CW） will be determined completely by the number and the type of BSUs and has nothing to do with the marshalling sequence. In particular, if the substrates are identical, the numbers of different types of BSUs are all the same and the middle two elements of BSUs constitute a cycle, then no matter whether the system is periodic, or quasiperiodic, or aperiodic, or unordered, or even random, it will be transparent at the CW. The conclusion is confirmed by the numerical results. Similar to the even layers of neighbourhood identical elements in TCD method, such a kind of optical BSU subsystem can also be decimated from the chain in the process of transmission investigation. There would be a potential application in the designing of some interesting optical devices.
