于2023年,对采自武夷山国家公园的松墨天牛(Monochamus alternatus)幼虫僵虫的寄生真菌进行分离培养,获得1株虫生真菌(BbWYZ-06),利用形态学观察和系统发育分析对该菌株进行鉴定,并测定该菌株的生物学特性以及对松墨天牛幼虫的致病性。...于2023年,对采自武夷山国家公园的松墨天牛(Monochamus alternatus)幼虫僵虫的寄生真菌进行分离培养,获得1株虫生真菌(BbWYZ-06),利用形态学观察和系统发育分析对该菌株进行鉴定,并测定该菌株的生物学特性以及对松墨天牛幼虫的致病性。结果表明,菌株BbWYZ-06的培养特征与白僵菌属球孢白僵菌较为一致,基于核糖体内转录间隔区(internal transcribed spacers, ITS)和RNA聚合酶Ⅱ的第2亚基(the second largest subunit of the nuclear RNA polymerase enzymeⅡ,RPB2)序列构建系统发育树,该菌株与球孢白僵菌(Beauveria bassiana)聚在同一分支,因此将菌株BbWYZ-06鉴定为球孢白僵菌。在25℃下,菌株BbWYZ-06在PDA培养基上的菌落生长速率为(2.54±0.25) mm·d^(-1),培养第15天时的产孢量为(2.11±0.23)×10^(8)个·cm^(-2)。菌株BbWYZ-06对松墨天牛幼虫具有较强的致病性,孢子悬浮液为1.0×10^(7)个·mL^(-1)时,松墨天牛幼虫第7天累计死亡率达93%,LT50值为3.95 d,在松墨天牛生物防治中具有良好的应用潜力。展开更多
为明确虫生真菌在玉米根际的分布情况,通过选择性培养基对采自安徽省涡阳、萧县、蒙城3地的玉米根际土壤中的虫生真菌进行了分离,并结合形态特征及r DNA ITS序列分析对分离的菌株进行了鉴定。结果表明,从玉米根际土壤中共分离得到39个...为明确虫生真菌在玉米根际的分布情况,通过选择性培养基对采自安徽省涡阳、萧县、蒙城3地的玉米根际土壤中的虫生真菌进行了分离,并结合形态特征及r DNA ITS序列分析对分离的菌株进行了鉴定。结果表明,从玉米根际土壤中共分离得到39个白僵菌菌株,经鉴定均为球孢白僵菌(Beauveria bassiana),且不同菌株间的菌落生长速度和分生孢子产生量存在显著差异。本研究对丰富玉米地下害虫及土传病害的生物防治资源具有重要的意义。展开更多
[Objective] This study was to investigate the relationship between biological characteristics of Beauveria bassiana (Bals.) Vuill and pathogenicity to Bombyx rnori L, with the aim to provide scientific basis for the...[Objective] This study was to investigate the relationship between biological characteristics of Beauveria bassiana (Bals.) Vuill and pathogenicity to Bombyx rnori L, with the aim to provide scientific basis for the control of white muscardine in Bombyx mori L. [Method] The strains isolated and purified from the 6 Beauveria bassiana biocontrol agents from all over the country and the 3 white muscardine silkworms collected from Guangxi provincial silkworm rearing areas were identified by the morphological observation and molecular biology technology. The pathogenicity of B. bassaina to silkworms was determined, and the biological characteristics such as growth diameter, sporulation and the extracellular protease activity of the different B. bassiana strains were compared. [Result] The isolated 9 strains were all B. bassaina (Bals.) Vuillemin, and all strains had high pathogenicity to silkworm, but with different pathogenicities. The growth diameter, sporulation and extracellular protease activity of different B. bassiana strains were also different, and showed correlation with the patheogenicity to silkworms. [Conclusion] B. bassiana spores production amount and exocellular protease activity had significant positive correlation with their pathogenicity to silkworm.展开更多
文摘于2023年,对采自武夷山国家公园的松墨天牛(Monochamus alternatus)幼虫僵虫的寄生真菌进行分离培养,获得1株虫生真菌(BbWYZ-06),利用形态学观察和系统发育分析对该菌株进行鉴定,并测定该菌株的生物学特性以及对松墨天牛幼虫的致病性。结果表明,菌株BbWYZ-06的培养特征与白僵菌属球孢白僵菌较为一致,基于核糖体内转录间隔区(internal transcribed spacers, ITS)和RNA聚合酶Ⅱ的第2亚基(the second largest subunit of the nuclear RNA polymerase enzymeⅡ,RPB2)序列构建系统发育树,该菌株与球孢白僵菌(Beauveria bassiana)聚在同一分支,因此将菌株BbWYZ-06鉴定为球孢白僵菌。在25℃下,菌株BbWYZ-06在PDA培养基上的菌落生长速率为(2.54±0.25) mm·d^(-1),培养第15天时的产孢量为(2.11±0.23)×10^(8)个·cm^(-2)。菌株BbWYZ-06对松墨天牛幼虫具有较强的致病性,孢子悬浮液为1.0×10^(7)个·mL^(-1)时,松墨天牛幼虫第7天累计死亡率达93%,LT50值为3.95 d,在松墨天牛生物防治中具有良好的应用潜力。
文摘为明确虫生真菌在玉米根际的分布情况,通过选择性培养基对采自安徽省涡阳、萧县、蒙城3地的玉米根际土壤中的虫生真菌进行了分离,并结合形态特征及r DNA ITS序列分析对分离的菌株进行了鉴定。结果表明,从玉米根际土壤中共分离得到39个白僵菌菌株,经鉴定均为球孢白僵菌(Beauveria bassiana),且不同菌株间的菌落生长速度和分生孢子产生量存在显著差异。本研究对丰富玉米地下害虫及土传病害的生物防治资源具有重要的意义。
基金Supported by the Scientific Research and Technology Development Planning Program of Guangxi Province(10169-08)the Program for the Construction of Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection(Guikeneng1001Z014)~~
文摘[Objective] This study was to investigate the relationship between biological characteristics of Beauveria bassiana (Bals.) Vuill and pathogenicity to Bombyx rnori L, with the aim to provide scientific basis for the control of white muscardine in Bombyx mori L. [Method] The strains isolated and purified from the 6 Beauveria bassiana biocontrol agents from all over the country and the 3 white muscardine silkworms collected from Guangxi provincial silkworm rearing areas were identified by the morphological observation and molecular biology technology. The pathogenicity of B. bassaina to silkworms was determined, and the biological characteristics such as growth diameter, sporulation and the extracellular protease activity of the different B. bassiana strains were compared. [Result] The isolated 9 strains were all B. bassaina (Bals.) Vuillemin, and all strains had high pathogenicity to silkworm, but with different pathogenicities. The growth diameter, sporulation and extracellular protease activity of different B. bassiana strains were also different, and showed correlation with the patheogenicity to silkworms. [Conclusion] B. bassiana spores production amount and exocellular protease activity had significant positive correlation with their pathogenicity to silkworm.