Only one herbicide mode of action (ALS inhibitor) is currently available to Ontario dry bean producers for soil-applied broadleaf weed control. Four field studies were conducted over two years (2014, 2015) to examine ...Only one herbicide mode of action (ALS inhibitor) is currently available to Ontario dry bean producers for soil-applied broadleaf weed control. Four field studies were conducted over two years (2014, 2015) to examine the tolerance of four market classes of dry beans to sulfentrazone (210 and 420 g·ai·ha<sup>-1</sup>) and pyroxasulfone (100 and 200 g·ai·ha<sup>-1</sup>) applied alone and in combination. The registration of these two herbicides would provide Ontario dry bean producers with two additional modes of action for broadleaf weed control. Pyroxasulfone caused up to 23%, 6%, 7% and 10% injury in adzuki, kidney, small red Mexican and white bean, respectively;sulfentrazone caused up to 51%, 12%, 15% and 44% injury and the combination caused up to 90%, 23%, 29% and 62% injury, respectively. Kidney and small red Mexican bean density, height, seed moisture content and yield were not affected. Pyroxasulfone (200 g·ai·ha<sup>-1</sup>) + sulfentrazone (420 g·ai·ha<sup>-1</sup>) reduced adzuki and white bean density, shoot dry weight, height and yield. This study concludes that pyroxasulfone (100 g·ai·ha<sup>-1</sup>) + sulfentrazone (210 g·ai·ha<sup>-1</sup>) applied PRE can be safely used to control weeds in Ontario kidney and small red Mexican bean production.展开更多
Genetic improvement of drought resistance is one of the main breeding goals for common bean,so molecular markers must be identified to facilitate drought resistance breeding.In this study,we evaluated the proline,treh...Genetic improvement of drought resistance is one of the main breeding goals for common bean,so molecular markers must be identified to facilitate drought resistance breeding.In this study,we evaluated the proline,trehalose,raffinose,and stachyose contents of 210 common bean accessions under two watering conditions and found large variations in all four.The coefficients of variation ranged from 21.21%for proline content to 78.69%for stachyose content under well-watered conditions,and from 20.11%for proline content to 50.08%for trehalose content under drought stress.According to our genome-wide association analysis,32 quantitative trait loci were associated with drought resistance,seven of which overlapped with known loci.Four hotspot regions were identified at Pv01,Pv07 and Pv11.A set of candidate genes was identified,including genes encoding MYB,bZIP,bHLH,ERF,and protein kinases.Among these genes,Phvul.001G189400,Phvul.007G273000 and Phvul.008G270500 were annotated as bZIP,ERF and WRKY,respectively.These genes are reportedly involved in drought stress responses in Arabidopsis thaliana and were induced by drought stress in common bean.Significant SNPs in six candidate gene regions formed different haplotypes,and phenotypic analysis revealed significant differences among the haplotypes.These results provide new insight into the genetic basis of drought resistance in common bean and reveal candidate genes and superior natural variations that will be useful for improving common bean.展开更多
Seed coat color affects the appearance and commodity quality of mung beans(Vigna radiata L.).The substances that affect mung bean seed coat color are mainly flavonoids,which have important medicinal value.Mapping the ...Seed coat color affects the appearance and commodity quality of mung beans(Vigna radiata L.).The substances that affect mung bean seed coat color are mainly flavonoids,which have important medicinal value.Mapping the seed coat color gene in mung beans would facilitate the development of new varieties and improve their value.In this study,an F2 mapping population consisting of 546 plants was constructed using Jilv9(black seed coat)and BIS9805(green seed coat).Using bulk segregated analysis(BSA)sequencing and kompetitive allele-specific PCR(KASP)markers,the candidate region related to seed coat color was finally narrowed to 0.66 Mb on chromosome(Chr.)4 and included eight candidate genes.Combined transcriptome and metabolome analyses showed that three of the eight candidate genes(LOC106758748,LOC106758747,and LOC106759075)were differentially expressed,which may have caused the differences in flavonoid metabolite content between Jilv9 and BIS9805.These findings can provide a research basis for cloning the genes related to seed coat color and accelerate molecular markerassisted selection breeding in mung beans.展开更多
The planting areas of mung bean are mostly arid and semi-arid areas, and lack of irrigation conditions. Many studies have reported that fertilization can increase drought resistance. In our previous research, optimize...The planting areas of mung bean are mostly arid and semi-arid areas, and lack of irrigation conditions. Many studies have reported that fertilization can increase drought resistance. In our previous research, optimized nitrogen (N), phosphorus (P) and potassium (K) combined fertilization model was established in mung bean. In the present study, the optimal fertilization was conducted in pot trails, and mung bean varieties Bailv9 and Bailv11 were used as materials, while the four water regimes, and three fertilization ratios of F120 (optimal fertilization), F100 (conventional fertilization), F50 (half of conventional fertilization) treatments were set, to compare each fertilization ratio effects and non-fertilization condition under each water regimes respectively. Under different water conditions, the investigation of N, P, and K effects of optimal fertilization showed that the yield of Bailv9 was not sensitive to water stress and had strong drought resistance;their water sensitivity index and drought resistance coefficient were BaiLv9 as Di = 0.89 and DC = 0.79. The yield of Bailv11 was sensitive to water stress, and their drought resistance was weak;their water sensitivity index and drought resistance coefficient were BL11 Di = 1.76 DC = 0.59, and under different water treatment conditions, Bailv9 and Bailv11 all had the best yield and other related traits increase in the F120 fertilization mode compared with other fertilization and non-fertilization conditions, and the average yield increases were 31.56% and 28.08%, respectively. The pot trails conduct the drought stress treatments in mung bean varieties Bailv9, Bailv11, Bailv935 and Bailv985 to determine the function of NPK optimized fertilization for improving plants growth in drought stress condition. Compared with the mung bean varieties treated with F50, F100, and F120, the yield of Bailv9 increased by 56.20%, 81.27%, and 107.22%, respectively;compared with that of F0, the yield of Bailv11 increased by 10.18%, 19.42%, and 45.88%, respectively;Bailv935 increased by 26.52%, 61.90%, 74.16% respectively, and Bailv985 increased by 23.78%, 56.92%, 87.62% respectively. The significant performances of optimized fertilization were also verified in 20 mung bean varieties in our filed trails. The research establishes a theoretical basis for introducing the model into production practice in the next step.展开更多
BACKGROUND Ileal atresia is a congenital abnormality where there is significant stenosis or complete absence of a portion of the ileum.The overall diagnostic accuracy of prenatal ultrasound in detecting jejunal and il...BACKGROUND Ileal atresia is a congenital abnormality where there is significant stenosis or complete absence of a portion of the ileum.The overall diagnostic accuracy of prenatal ultrasound in detecting jejunal and ileal atresia is low.We report a case of ileal atresia diagnosed prenatally by ultrasound examination with the“keyboard sign”and“coffee bean sign”.CASE SUMMARY We report a case of ileal atresia diagnosed in utero at 31 weeks'of gestation.Prenatal ultrasound examination revealed two rows of intestines arranged in an‘S’shape in the middle abdomen.The inner diameters were 1.7 cm and 1.6 cm,respectively.A typical“keyboard sign”was observed.The intestine canal behind the“keyboard sign”showed an irregular strong echo.There was no normal intestinal wall structure,showing a typical“coffee bean sign”.Termination of the pregnancy and autopsy findings confirmed the diagnosis.CONCLUSION The prenatal diagnosis of ileal atresia is difficult.The sonographic features of the“keyboard sign”and“coffee bean sign”are helpful in diagnosing the location of congenital jejunal and ileal atresia.展开更多
Tylosema esculentum (Burch.) A. Schreib. (Marama bean), referred to as marama in sections of this article, is an obligate outcrossing native plant with a yield potential of 2 ton/hectare which grows naturally in the d...Tylosema esculentum (Burch.) A. Schreib. (Marama bean), referred to as marama in sections of this article, is an obligate outcrossing native plant with a yield potential of 2 ton/hectare which grows naturally in the deep sandy soils of the Kalahari Desert. It has adapted to the low precipitation levels in that agro-ecosystem. Marama serves as a staple food for the San and Bantus in that area. In Namibia, in the past you could find wild stands of marama in the Khomas region, Omaheke region, and the Otjozondjupa region without must struggle. It is renowned for its brown seeds, which are rich in high-quality oils and proteins. The tuberous root contains a significant amount of starch. The objective of domesticating orphaned marama is to provide farmers in this climate change-prone region with a viable alternative for food and nutrition security. This program, initiated in 2008 with an open-minded mindset, required swift implementation using harsh and occasionally unconventional methods. To introduce indigenous tools for resource-poor farmers, the domestication program prioritized the utilization of farmer-participatory methodologies. It was crucial to integrate old and new approaches to ensure learning from past and present experiences, leading to innovative solutions. There is little research and development of native crops in Africa because most of the currently cultivates crops were brought for use from abroad. Only a few numbers of indeginous African crops can be named. The arid Kalahari region, susceptible to climate change, necessitates the revival of indigenous crops like marama, which are resilient and well-adapted to the region’s conditions and have thrived for centuries. In many discussions regarding the health and nutrition of Africa, the recommendation to consume traditional foods to avoid exposure to modern foods, which may not be genetically compatible, is frequently emphasized. Regardless of their validity, these opinions must be acknowledged, and steps need to be taken to ensure a positive legacy for future generations. However, this chapter will address the limitations and challenges that exist in this regard. This article will summarize the progress made in the domestication program of the marama bean in Namibia thus far. Furthermore, this article will highlight the challenges that have been faced during the domestication journey for marama bean and other orphaned crops. The domestication program commenced with a broad germplasm collection, characterization, and preselection for breeding. Crop selection in this program was influenced by climate change-related concerns of shorter and uncertain rain seasons, and recurrent droughts. Selection included but was not limited to identifying marama genotypes with superior characteristics, early germination and many seeds per pod were among some of the identified and selected characteristics. The Namibia University of Science and Technology (NUST) has compiled a list of potential marama bean varieties and is currently testing marama seeds in anticipation of their introduction as a new crop alternative with good adaptation to the effects of climate change, since conventional crops like maize underperform due to persistent droughts. Marama bean, if properly developed, holds significant potential to address issues of hunger and malnutrition in arid regions of Southern Africa and other similar territories. The findings presented here are the result of ongoing field research and experiments conducted at multiple sites using superior marama bean varieties.展开更多
Infant malnutrition is a significant issue in Côte d’Ivoire, and this study aims to address it by formulating infant flours using local ingredients. Fermentation, germination, and malting methods were used to en...Infant malnutrition is a significant issue in Côte d’Ivoire, and this study aims to address it by formulating infant flours using local ingredients. Fermentation, germination, and malting methods were used to enhance the quality of six formulated flours, all based on Fonio and supplemented with Bambara groundnut, African locust bean fruit pulp, and cashew kernels. Results showed that Fonio had the highest carbohydrate content, while Bambara groundnut and Cashew kernels were rich in protein and lipid content. African locust bean fruit pulp was rich in fiber and Vitamin C, with a high β-carotene value. The cashew kernel had the highest energy value. Regarding mineral composition, African locust bean fruit pulp had the highest potassium content, while Bambara groundnut and African locust bean fruit pulp were rich in sodium. Cashew kernel and Fonio had higher iron and calcium content. Bambara groundnut had a higher zinc content, while cashew kernel had a higher magnesium content. The formulated flours made from fermented Fonio grains and enriched with Bambara groundnut, African locust bean fruit pulp, and cashew kernel had varying protein, fiber, carbohydrate, ash, and fat contents. The flour formulated with sprouted Fonio and enriched with the same ingredients had higher protein content and energy value than the other fermented seed-based flours. The mixed flours produced with fermented seeds and the flour produced from sprouted seeds met international standards. Overall, these findings offer valuable insights into the nutritional composition of the formulated flours and their potential to combat infant malnutrition in Côte d’Ivoire.展开更多
Limited information is available on the sensitivity of dry beans to </span><span style="font-family:Verdana;">tribenuron and carfentrazone applied preplant (PP).</span><span style="...Limited information is available on the sensitivity of dry beans to </span><span style="font-family:Verdana;">tribenuron and carfentrazone applied preplant (PP).</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">Four field trials were conducted at Exeter and Ridgetown, ON, Canada in 2019 and 2020 to determine the toler</span><span style="font-family:Verdana;">ance of azuki, kidney, small red and white beans to glyphosate (1800</span><span style="font-family:Verdana;"> g·ae·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) + tribenuron (15 g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ai</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">), glyphosate (1800 </span><a name="_Hlk64540918"></a><span style="font-family:Verdana;">g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ae</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) + carfentrazone (35 </span><span style="font-family:Verdana;">g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ai</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) and glyphosate (1800 g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ae</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) + tribenuron (15 g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ai</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) + car</span><span style="font-family:Verdana;">fentrazone (35 g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ai</span><span style="font-family:Verdana;">·</span><span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) applied PP 1 - 2 days prior to seeding dry beans.</span></span><span style="font-family:Verdana;"> Glyphosate + tribenuron, glyphosate + carfentrazone, and glyphosate + tribenuron + carfentrazone, applied PP, caused 5%, 5% and 9% bean injury at 1 WAE;7%, 6% and 10% bean injury at 2 WAE and 6%, 5% and 8% bean injury at 4 WAE, respectively. At 8 WAE, there was 0, 1% and 4% injury in azuki bean;1%, 2% and 2% injury in kidney bean;3%, 2% and 3% injury in small red bean;and 6%, 3% and 2% injury in white bean with glyphosate + tribenuron, glyphosate + carfentrazone, and glyphosate + tribenuron + carfentrazone applied PP, respectively. The injury was significantly greater with glyphosate + tribenuron in small red and white beans compared to the azuki and kidney beans. There was no difference between injury levels among market classes of dry been with glyphosate + carfentrazone or glyphosate + tribenuron + carfentrazone applied PP. There was no effect of glyphosate + tribenuron, glyphosate + carfentrazone and glyphosate + tribenuron + carfentrazone on dry bean plant stand, maturity and seed yield. However, dry bean biomass was reduced as much as 15% with glyphosate + tribenuron and 13% </span><span style="font-family:Verdana;">with glyphosate + tribenuron + carfentrazone compared to the untreated</span><span style="font-family:Verdana;"> control. Dry bean height was reduced 4% with glyphosate + tribenuron + carfentrazone but was not affected with other treatments. Based on these results, there is potential for using glyphosate plus tribenuron or carfentrazone for preplant weed management in dry bean production.展开更多
Ontario dry bean growers are currently limited to ALS inhibitor herbicides for soilapplied broadleaf weed control;therefore another mode of action is needed. Sulfentrazone is a PPO inhibitor herbicide that has activit...Ontario dry bean growers are currently limited to ALS inhibitor herbicides for soilapplied broadleaf weed control;therefore another mode of action is needed. Sulfentrazone is a PPO inhibitor herbicide that has activity on some annual grass and broadleaf weed species. Four field trials were conducted over two years (2014, 2015) to determine the tolerance of four commonly grown dry bean market classes (adzuki, kidney, small red Mexican and white bean) to PRE applications of sulfentrazone at 140, 210, 280 and 420 g·ai·ha<sup>-1</sup>. Crop injury, plant height, plant density, shoot biomass, seed moisture content and yield were examined. Sulfentrazone (420 g·ai·ha<sup>-1</sup>) caused up to 74%, 22%, 30%, and 57% injury in adzuki, kidney, small red Mexican and white bean, respectively. Plant density, height and yield were not reduced for kidney or small red Mexican bean. Sulfentrazone (420 g·ai·ha<sup>-1</sup>) reduced white bean plant density, height and yield by 28%, 29% and 29%, respectively;and reduced adzuki bean plant density, height and yield by 51%, 34% and 57%, respectively. Overall, kidney and small red Mexican bean were the most tolerant to sulfentrazone, followed by white bean, and then adzuki. This study determined sulfentrazone applied PRE is safe for Ontario kidney bean and small red Mexican bean crops.展开更多
The high-efficiency planting mode for five crops a year of fresh edible "faba bean/spring maize+soybean-autumn maize/autumn soybean" was introduced, and its yield and economic benefits were compared with the planti...The high-efficiency planting mode for five crops a year of fresh edible "faba bean/spring maize+soybean-autumn maize/autumn soybean" was introduced, and its yield and economic benefits were compared with the planting mode of three crops a year of "faba bean-spring maize/red bean". The results showed that the planting method for fresh edible "faba bean/spring maize+soybean-autumn maize/autumn soybean" was much easier to operate with the input-output ratio of about185.6%, and its yield and economic benefits were 2.09 and 1.83 times of that of the planting mode for three crops a year, significantly improving the agricultural yield and income of farmers. In addition, the cropping index of the planting mode for fresh edible fresh edible "faba bean/spring maize +soybean-autumn maize/autumn soybean" reached up to 350%, and planting faba bean once a year and soybean twice a year could make the biological fixation amount of nitrogen increase 350-450kg/m^2, which equaled to up to 700 kg/m^2 of urea, showing significant ecological and social benefits. Based on the comparison results, the high-yield culture techniques of the planting mode of fresh edible "faba bean/spring maize+soybean-autumn maize/autumn soybean" were summarized.展开更多
Soybean is one of the most important sources of edible oil and proteins in the world. However, it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production. The fungal disease c...Soybean is one of the most important sources of edible oil and proteins in the world. However, it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production. The fungal disease can be effectively controlled by breeding plant cultivars with genetic transformation. In this study, the resistance to Phytophthora sojae of five bivalent transgenic soybean lines was identified using the hypocotyls inoculation technique. The lines were the T2 of the transgenic soybean which were transformed with kidney bean chitinase gene and barley ribosome inactivating protein gene, and were positive by Southern Blot analysis. The resistance difference was studied through comparing the death percentage of transgenic soybean with the control. The results showed that four lines were more resistant to P sojae, whereas other one had no significant difference in comparison with the control. These transgenic soybean lines with enhanced resistance to P sojae will be useful in soybean resistance breeding.展开更多
In coffee breeding practice, two quantitative traits, namely cherry and green bean characters are the important phenotypic selection index. The synchronous of cherry maturation, size, weight and shape of green bean ar...In coffee breeding practice, two quantitative traits, namely cherry and green bean characters are the important phenotypic selection index. The synchronous of cherry maturation, size, weight and shape of green bean are desirable traits for the future breeding. In order to increase the breeding efficiency, a set of quantitative trait loci (QTLs) analysis controlling these traits was carried out. The QTL analysis was performanced in cross pollinated population of Coffea canephora using single nucleotide polymorphisms (SNPs) and simple sequence repeats (SSRs) markers. Totally 12 QTLs determined desirable cherry and green bean traits were identified on 9 linkage groups (LG), where these QTLs mapped forming clusters in 11 different chromosomal regions. The desirable traits which can be detected by their QTLs are synchronous of cherry maturation (LG B and LG G), cherry size (LG A, LG F and LG G), bean shape (LG B, LG D and LG J), bean weight (LG H), bean size (LG A and LG E) and cherry-bean size (LG K) The gene action of these QTLs was dominance or an interaction of alleles (epistasis between alleles at the studied locus) effect. The yielded linkage map and the detected QTL can provide a tool for further genetic analysis of two traits and be potential for maker-assisted selection in C. canephora breeding.展开更多
This paper introduced the definition and importance of combination of sta-ple food. With broad bean nutrition flour as a sample, the preparation of the combi-nation of staple food was also described. In addition, the ...This paper introduced the definition and importance of combination of sta-ple food. With broad bean nutrition flour as a sample, the preparation of the combi-nation of staple food was also described. In addition, the main nutritional value of broad bean nutrition flour was introduced. Compared with those of other single flours, the nutritional value of broad bean nutrition flour was improved. Moreover, the nutrients in the broad bean nutrition flour would not be destroyed during the processing and preparation of staple food, and the processed steamed bread and raw noodle are more characteristic. The application value and prospects of broad bean nutrition flour, as a combination of staple food, were further discussed.展开更多
文摘Only one herbicide mode of action (ALS inhibitor) is currently available to Ontario dry bean producers for soil-applied broadleaf weed control. Four field studies were conducted over two years (2014, 2015) to examine the tolerance of four market classes of dry beans to sulfentrazone (210 and 420 g·ai·ha<sup>-1</sup>) and pyroxasulfone (100 and 200 g·ai·ha<sup>-1</sup>) applied alone and in combination. The registration of these two herbicides would provide Ontario dry bean producers with two additional modes of action for broadleaf weed control. Pyroxasulfone caused up to 23%, 6%, 7% and 10% injury in adzuki, kidney, small red Mexican and white bean, respectively;sulfentrazone caused up to 51%, 12%, 15% and 44% injury and the combination caused up to 90%, 23%, 29% and 62% injury, respectively. Kidney and small red Mexican bean density, height, seed moisture content and yield were not affected. Pyroxasulfone (200 g·ai·ha<sup>-1</sup>) + sulfentrazone (420 g·ai·ha<sup>-1</sup>) reduced adzuki and white bean density, shoot dry weight, height and yield. This study concludes that pyroxasulfone (100 g·ai·ha<sup>-1</sup>) + sulfentrazone (210 g·ai·ha<sup>-1</sup>) applied PRE can be safely used to control weeds in Ontario kidney and small red Mexican bean production.
基金supported by the National Key Research and Development Program of China(2019YFD1001300 and 2019YFD1001305)the Fundamental Research Funds for the Central Universities,China(SWUKQ22042)+1 种基金the China Agriculture Research System of MOF and MARA(CARS-08)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences。
文摘Genetic improvement of drought resistance is one of the main breeding goals for common bean,so molecular markers must be identified to facilitate drought resistance breeding.In this study,we evaluated the proline,trehalose,raffinose,and stachyose contents of 210 common bean accessions under two watering conditions and found large variations in all four.The coefficients of variation ranged from 21.21%for proline content to 78.69%for stachyose content under well-watered conditions,and from 20.11%for proline content to 50.08%for trehalose content under drought stress.According to our genome-wide association analysis,32 quantitative trait loci were associated with drought resistance,seven of which overlapped with known loci.Four hotspot regions were identified at Pv01,Pv07 and Pv11.A set of candidate genes was identified,including genes encoding MYB,bZIP,bHLH,ERF,and protein kinases.Among these genes,Phvul.001G189400,Phvul.007G273000 and Phvul.008G270500 were annotated as bZIP,ERF and WRKY,respectively.These genes are reportedly involved in drought stress responses in Arabidopsis thaliana and were induced by drought stress in common bean.Significant SNPs in six candidate gene regions formed different haplotypes,and phenotypic analysis revealed significant differences among the haplotypes.These results provide new insight into the genetic basis of drought resistance in common bean and reveal candidate genes and superior natural variations that will be useful for improving common bean.
基金supported by the National Natural Science Foundation of China(32301928)the Basic Research Program of Shanxi Province,China(20210302124504)+3 种基金the China Agriculture Research System of MOF and MARA-Food Legumes(CARS08-G10)the National Laboratory Project of Coarse Grain Germplasm Resources Innovation and Molecular Breeding,China(K462202040-01)the Ph D of Shanxi Agricultural University Scientific Research Start-up Project,China(2021BQ43)the Scientific Research Project of Shanxi Agricultural University,China(YZGC098)。
文摘Seed coat color affects the appearance and commodity quality of mung beans(Vigna radiata L.).The substances that affect mung bean seed coat color are mainly flavonoids,which have important medicinal value.Mapping the seed coat color gene in mung beans would facilitate the development of new varieties and improve their value.In this study,an F2 mapping population consisting of 546 plants was constructed using Jilv9(black seed coat)and BIS9805(green seed coat).Using bulk segregated analysis(BSA)sequencing and kompetitive allele-specific PCR(KASP)markers,the candidate region related to seed coat color was finally narrowed to 0.66 Mb on chromosome(Chr.)4 and included eight candidate genes.Combined transcriptome and metabolome analyses showed that three of the eight candidate genes(LOC106758748,LOC106758747,and LOC106759075)were differentially expressed,which may have caused the differences in flavonoid metabolite content between Jilv9 and BIS9805.These findings can provide a research basis for cloning the genes related to seed coat color and accelerate molecular markerassisted selection breeding in mung beans.
文摘The planting areas of mung bean are mostly arid and semi-arid areas, and lack of irrigation conditions. Many studies have reported that fertilization can increase drought resistance. In our previous research, optimized nitrogen (N), phosphorus (P) and potassium (K) combined fertilization model was established in mung bean. In the present study, the optimal fertilization was conducted in pot trails, and mung bean varieties Bailv9 and Bailv11 were used as materials, while the four water regimes, and three fertilization ratios of F120 (optimal fertilization), F100 (conventional fertilization), F50 (half of conventional fertilization) treatments were set, to compare each fertilization ratio effects and non-fertilization condition under each water regimes respectively. Under different water conditions, the investigation of N, P, and K effects of optimal fertilization showed that the yield of Bailv9 was not sensitive to water stress and had strong drought resistance;their water sensitivity index and drought resistance coefficient were BaiLv9 as Di = 0.89 and DC = 0.79. The yield of Bailv11 was sensitive to water stress, and their drought resistance was weak;their water sensitivity index and drought resistance coefficient were BL11 Di = 1.76 DC = 0.59, and under different water treatment conditions, Bailv9 and Bailv11 all had the best yield and other related traits increase in the F120 fertilization mode compared with other fertilization and non-fertilization conditions, and the average yield increases were 31.56% and 28.08%, respectively. The pot trails conduct the drought stress treatments in mung bean varieties Bailv9, Bailv11, Bailv935 and Bailv985 to determine the function of NPK optimized fertilization for improving plants growth in drought stress condition. Compared with the mung bean varieties treated with F50, F100, and F120, the yield of Bailv9 increased by 56.20%, 81.27%, and 107.22%, respectively;compared with that of F0, the yield of Bailv11 increased by 10.18%, 19.42%, and 45.88%, respectively;Bailv935 increased by 26.52%, 61.90%, 74.16% respectively, and Bailv985 increased by 23.78%, 56.92%, 87.62% respectively. The significant performances of optimized fertilization were also verified in 20 mung bean varieties in our filed trails. The research establishes a theoretical basis for introducing the model into production practice in the next step.
文摘BACKGROUND Ileal atresia is a congenital abnormality where there is significant stenosis or complete absence of a portion of the ileum.The overall diagnostic accuracy of prenatal ultrasound in detecting jejunal and ileal atresia is low.We report a case of ileal atresia diagnosed prenatally by ultrasound examination with the“keyboard sign”and“coffee bean sign”.CASE SUMMARY We report a case of ileal atresia diagnosed in utero at 31 weeks'of gestation.Prenatal ultrasound examination revealed two rows of intestines arranged in an‘S’shape in the middle abdomen.The inner diameters were 1.7 cm and 1.6 cm,respectively.A typical“keyboard sign”was observed.The intestine canal behind the“keyboard sign”showed an irregular strong echo.There was no normal intestinal wall structure,showing a typical“coffee bean sign”.Termination of the pregnancy and autopsy findings confirmed the diagnosis.CONCLUSION The prenatal diagnosis of ileal atresia is difficult.The sonographic features of the“keyboard sign”and“coffee bean sign”are helpful in diagnosing the location of congenital jejunal and ileal atresia.
文摘Tylosema esculentum (Burch.) A. Schreib. (Marama bean), referred to as marama in sections of this article, is an obligate outcrossing native plant with a yield potential of 2 ton/hectare which grows naturally in the deep sandy soils of the Kalahari Desert. It has adapted to the low precipitation levels in that agro-ecosystem. Marama serves as a staple food for the San and Bantus in that area. In Namibia, in the past you could find wild stands of marama in the Khomas region, Omaheke region, and the Otjozondjupa region without must struggle. It is renowned for its brown seeds, which are rich in high-quality oils and proteins. The tuberous root contains a significant amount of starch. The objective of domesticating orphaned marama is to provide farmers in this climate change-prone region with a viable alternative for food and nutrition security. This program, initiated in 2008 with an open-minded mindset, required swift implementation using harsh and occasionally unconventional methods. To introduce indigenous tools for resource-poor farmers, the domestication program prioritized the utilization of farmer-participatory methodologies. It was crucial to integrate old and new approaches to ensure learning from past and present experiences, leading to innovative solutions. There is little research and development of native crops in Africa because most of the currently cultivates crops were brought for use from abroad. Only a few numbers of indeginous African crops can be named. The arid Kalahari region, susceptible to climate change, necessitates the revival of indigenous crops like marama, which are resilient and well-adapted to the region’s conditions and have thrived for centuries. In many discussions regarding the health and nutrition of Africa, the recommendation to consume traditional foods to avoid exposure to modern foods, which may not be genetically compatible, is frequently emphasized. Regardless of their validity, these opinions must be acknowledged, and steps need to be taken to ensure a positive legacy for future generations. However, this chapter will address the limitations and challenges that exist in this regard. This article will summarize the progress made in the domestication program of the marama bean in Namibia thus far. Furthermore, this article will highlight the challenges that have been faced during the domestication journey for marama bean and other orphaned crops. The domestication program commenced with a broad germplasm collection, characterization, and preselection for breeding. Crop selection in this program was influenced by climate change-related concerns of shorter and uncertain rain seasons, and recurrent droughts. Selection included but was not limited to identifying marama genotypes with superior characteristics, early germination and many seeds per pod were among some of the identified and selected characteristics. The Namibia University of Science and Technology (NUST) has compiled a list of potential marama bean varieties and is currently testing marama seeds in anticipation of their introduction as a new crop alternative with good adaptation to the effects of climate change, since conventional crops like maize underperform due to persistent droughts. Marama bean, if properly developed, holds significant potential to address issues of hunger and malnutrition in arid regions of Southern Africa and other similar territories. The findings presented here are the result of ongoing field research and experiments conducted at multiple sites using superior marama bean varieties.
文摘Infant malnutrition is a significant issue in Côte d’Ivoire, and this study aims to address it by formulating infant flours using local ingredients. Fermentation, germination, and malting methods were used to enhance the quality of six formulated flours, all based on Fonio and supplemented with Bambara groundnut, African locust bean fruit pulp, and cashew kernels. Results showed that Fonio had the highest carbohydrate content, while Bambara groundnut and Cashew kernels were rich in protein and lipid content. African locust bean fruit pulp was rich in fiber and Vitamin C, with a high β-carotene value. The cashew kernel had the highest energy value. Regarding mineral composition, African locust bean fruit pulp had the highest potassium content, while Bambara groundnut and African locust bean fruit pulp were rich in sodium. Cashew kernel and Fonio had higher iron and calcium content. Bambara groundnut had a higher zinc content, while cashew kernel had a higher magnesium content. The formulated flours made from fermented Fonio grains and enriched with Bambara groundnut, African locust bean fruit pulp, and cashew kernel had varying protein, fiber, carbohydrate, ash, and fat contents. The flour formulated with sprouted Fonio and enriched with the same ingredients had higher protein content and energy value than the other fermented seed-based flours. The mixed flours produced with fermented seeds and the flour produced from sprouted seeds met international standards. Overall, these findings offer valuable insights into the nutritional composition of the formulated flours and their potential to combat infant malnutrition in Côte d’Ivoire.
文摘Limited information is available on the sensitivity of dry beans to </span><span style="font-family:Verdana;">tribenuron and carfentrazone applied preplant (PP).</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">Four field trials were conducted at Exeter and Ridgetown, ON, Canada in 2019 and 2020 to determine the toler</span><span style="font-family:Verdana;">ance of azuki, kidney, small red and white beans to glyphosate (1800</span><span style="font-family:Verdana;"> g·ae·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) + tribenuron (15 g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ai</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">), glyphosate (1800 </span><a name="_Hlk64540918"></a><span style="font-family:Verdana;">g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ae</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) + carfentrazone (35 </span><span style="font-family:Verdana;">g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ai</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) and glyphosate (1800 g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ae</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) + tribenuron (15 g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ai</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) + car</span><span style="font-family:Verdana;">fentrazone (35 g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ai</span><span style="font-family:Verdana;">·</span><span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) applied PP 1 - 2 days prior to seeding dry beans.</span></span><span style="font-family:Verdana;"> Glyphosate + tribenuron, glyphosate + carfentrazone, and glyphosate + tribenuron + carfentrazone, applied PP, caused 5%, 5% and 9% bean injury at 1 WAE;7%, 6% and 10% bean injury at 2 WAE and 6%, 5% and 8% bean injury at 4 WAE, respectively. At 8 WAE, there was 0, 1% and 4% injury in azuki bean;1%, 2% and 2% injury in kidney bean;3%, 2% and 3% injury in small red bean;and 6%, 3% and 2% injury in white bean with glyphosate + tribenuron, glyphosate + carfentrazone, and glyphosate + tribenuron + carfentrazone applied PP, respectively. The injury was significantly greater with glyphosate + tribenuron in small red and white beans compared to the azuki and kidney beans. There was no difference between injury levels among market classes of dry been with glyphosate + carfentrazone or glyphosate + tribenuron + carfentrazone applied PP. There was no effect of glyphosate + tribenuron, glyphosate + carfentrazone and glyphosate + tribenuron + carfentrazone on dry bean plant stand, maturity and seed yield. However, dry bean biomass was reduced as much as 15% with glyphosate + tribenuron and 13% </span><span style="font-family:Verdana;">with glyphosate + tribenuron + carfentrazone compared to the untreated</span><span style="font-family:Verdana;"> control. Dry bean height was reduced 4% with glyphosate + tribenuron + carfentrazone but was not affected with other treatments. Based on these results, there is potential for using glyphosate plus tribenuron or carfentrazone for preplant weed management in dry bean production.
文摘Ontario dry bean growers are currently limited to ALS inhibitor herbicides for soilapplied broadleaf weed control;therefore another mode of action is needed. Sulfentrazone is a PPO inhibitor herbicide that has activity on some annual grass and broadleaf weed species. Four field trials were conducted over two years (2014, 2015) to determine the tolerance of four commonly grown dry bean market classes (adzuki, kidney, small red Mexican and white bean) to PRE applications of sulfentrazone at 140, 210, 280 and 420 g·ai·ha<sup>-1</sup>. Crop injury, plant height, plant density, shoot biomass, seed moisture content and yield were examined. Sulfentrazone (420 g·ai·ha<sup>-1</sup>) caused up to 74%, 22%, 30%, and 57% injury in adzuki, kidney, small red Mexican and white bean, respectively. Plant density, height and yield were not reduced for kidney or small red Mexican bean. Sulfentrazone (420 g·ai·ha<sup>-1</sup>) reduced white bean plant density, height and yield by 28%, 29% and 29%, respectively;and reduced adzuki bean plant density, height and yield by 51%, 34% and 57%, respectively. Overall, kidney and small red Mexican bean were the most tolerant to sulfentrazone, followed by white bean, and then adzuki. This study determined sulfentrazone applied PRE is safe for Ontario kidney bean and small red Mexican bean crops.
基金Supported by the China Agriculture Research System(CARS-09)the Technological Innovation and Industrialization Project for the Rural Area of Nantong City,Jiangsu Province(H12014012)~~
文摘The high-efficiency planting mode for five crops a year of fresh edible "faba bean/spring maize+soybean-autumn maize/autumn soybean" was introduced, and its yield and economic benefits were compared with the planting mode of three crops a year of "faba bean-spring maize/red bean". The results showed that the planting method for fresh edible "faba bean/spring maize+soybean-autumn maize/autumn soybean" was much easier to operate with the input-output ratio of about185.6%, and its yield and economic benefits were 2.09 and 1.83 times of that of the planting mode for three crops a year, significantly improving the agricultural yield and income of farmers. In addition, the cropping index of the planting mode for fresh edible fresh edible "faba bean/spring maize +soybean-autumn maize/autumn soybean" reached up to 350%, and planting faba bean once a year and soybean twice a year could make the biological fixation amount of nitrogen increase 350-450kg/m^2, which equaled to up to 700 kg/m^2 of urea, showing significant ecological and social benefits. Based on the comparison results, the high-yield culture techniques of the planting mode of fresh edible "faba bean/spring maize+soybean-autumn maize/autumn soybean" were summarized.
基金Supported by the National Items of Research and Industrial Development of Transgenic Plants(J99-B-013)
文摘Soybean is one of the most important sources of edible oil and proteins in the world. However, it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production. The fungal disease can be effectively controlled by breeding plant cultivars with genetic transformation. In this study, the resistance to Phytophthora sojae of five bivalent transgenic soybean lines was identified using the hypocotyls inoculation technique. The lines were the T2 of the transgenic soybean which were transformed with kidney bean chitinase gene and barley ribosome inactivating protein gene, and were positive by Southern Blot analysis. The resistance difference was studied through comparing the death percentage of transgenic soybean with the control. The results showed that four lines were more resistant to P sojae, whereas other one had no significant difference in comparison with the control. These transgenic soybean lines with enhanced resistance to P sojae will be useful in soybean resistance breeding.
文摘In coffee breeding practice, two quantitative traits, namely cherry and green bean characters are the important phenotypic selection index. The synchronous of cherry maturation, size, weight and shape of green bean are desirable traits for the future breeding. In order to increase the breeding efficiency, a set of quantitative trait loci (QTLs) analysis controlling these traits was carried out. The QTL analysis was performanced in cross pollinated population of Coffea canephora using single nucleotide polymorphisms (SNPs) and simple sequence repeats (SSRs) markers. Totally 12 QTLs determined desirable cherry and green bean traits were identified on 9 linkage groups (LG), where these QTLs mapped forming clusters in 11 different chromosomal regions. The desirable traits which can be detected by their QTLs are synchronous of cherry maturation (LG B and LG G), cherry size (LG A, LG F and LG G), bean shape (LG B, LG D and LG J), bean weight (LG H), bean size (LG A and LG E) and cherry-bean size (LG K) The gene action of these QTLs was dominance or an interaction of alleles (epistasis between alleles at the studied locus) effect. The yielded linkage map and the detected QTL can provide a tool for further genetic analysis of two traits and be potential for maker-assisted selection in C. canephora breeding.
基金Supported by Jiangsu Agricultural Science and Technology Innovation Fund[CX(13)3084]Jiangsu Province Science and Technology Support Program,China(BE2013352)~~
文摘This paper introduced the definition and importance of combination of sta-ple food. With broad bean nutrition flour as a sample, the preparation of the combi-nation of staple food was also described. In addition, the main nutritional value of broad bean nutrition flour was introduced. Compared with those of other single flours, the nutritional value of broad bean nutrition flour was improved. Moreover, the nutrients in the broad bean nutrition flour would not be destroyed during the processing and preparation of staple food, and the processed steamed bread and raw noodle are more characteristic. The application value and prospects of broad bean nutrition flour, as a combination of staple food, were further discussed.