Objective:To combine with transdermal drug delivery using microneedle to simulate the bee venom therapy to evaluate the permeation of bee venom gel.Methods:In this study,the sodium urate and LPS were used on rats and ...Objective:To combine with transdermal drug delivery using microneedle to simulate the bee venom therapy to evaluate the permeation of bee venom gel.Methods:In this study,the sodium urate and LPS were used on rats and mice to construct the model.Bee venom gelemicroneedle combination effect on the model is to determine the role of microneedle gel permeation by observing inflammation factors.Results:Compared with the model group,the bee venom gelemicroneedle combination group can reduce the level of serum nitric oxide of the acute gouty inflammation model caused by sodium urate,and on LPS induced mouse model of acute inflammation effect and the micro.Conclusions:Bee venom can significantly suppress the occurrence of gouty arthritis inflammation in rats and mice LPS inflammatory reaction.Choose the 750 mm microneedle with 10N force on skin about 3 minutes,bee venom can play the optimal role,and the anti-inflammatory effect is obvious.Microneedles can promote the percutaneous absorption of the active macromolecules bee venom gel.展开更多
目的:通过观察蜂毒注射对佐剂性关节炎(AA)大鼠治疗作用的影响,研究蜜蜂蜂毒提取物(BV)对AA大鼠的治疗作用和作用机制。方法:采用Wistar雄性成年大鼠30只,于右后足垫皮下注射CFA 0.1 m L造模,分为模型组、蜂毒组。另设正常组,于右后足...目的:通过观察蜂毒注射对佐剂性关节炎(AA)大鼠治疗作用的影响,研究蜜蜂蜂毒提取物(BV)对AA大鼠的治疗作用和作用机制。方法:采用Wistar雄性成年大鼠30只,于右后足垫皮下注射CFA 0.1 m L造模,分为模型组、蜂毒组。另设正常组,于右后足垫皮下注射生理盐水0.1 m L。每组10只。蜂毒组大鼠每日蜂毒注射0.3 mg治疗。记录所有大鼠足跖厚度、痛阈,治疗后采用ELISA检测大鼠外周血血清IL-1β水平。结果:治疗后蜂毒组足跖厚度均低于模型组,痛阈高于模型组;进一步采用ELISA检测血清中IL-1β水平显示蜂毒组血清IL-1β水平比模型组低。结论:蜂毒穴位注射足三里能减轻足跖肿胀,提高痛阈,同时抑制炎性细胞因子IL-1β的转录和表达,对AA大鼠有效。展开更多
基金the Beijing Science and Technology New Star Program(grant No.2015A048)the Young Teacher Special of Beijing University of Chinese Medicine(1000061221025)National Science and Technology Major New Drug Projects(2014ZX09301306-009).
文摘Objective:To combine with transdermal drug delivery using microneedle to simulate the bee venom therapy to evaluate the permeation of bee venom gel.Methods:In this study,the sodium urate and LPS were used on rats and mice to construct the model.Bee venom gelemicroneedle combination effect on the model is to determine the role of microneedle gel permeation by observing inflammation factors.Results:Compared with the model group,the bee venom gelemicroneedle combination group can reduce the level of serum nitric oxide of the acute gouty inflammation model caused by sodium urate,and on LPS induced mouse model of acute inflammation effect and the micro.Conclusions:Bee venom can significantly suppress the occurrence of gouty arthritis inflammation in rats and mice LPS inflammatory reaction.Choose the 750 mm microneedle with 10N force on skin about 3 minutes,bee venom can play the optimal role,and the anti-inflammatory effect is obvious.Microneedles can promote the percutaneous absorption of the active macromolecules bee venom gel.
文摘目的:通过观察蜂毒注射对佐剂性关节炎(AA)大鼠治疗作用的影响,研究蜜蜂蜂毒提取物(BV)对AA大鼠的治疗作用和作用机制。方法:采用Wistar雄性成年大鼠30只,于右后足垫皮下注射CFA 0.1 m L造模,分为模型组、蜂毒组。另设正常组,于右后足垫皮下注射生理盐水0.1 m L。每组10只。蜂毒组大鼠每日蜂毒注射0.3 mg治疗。记录所有大鼠足跖厚度、痛阈,治疗后采用ELISA检测大鼠外周血血清IL-1β水平。结果:治疗后蜂毒组足跖厚度均低于模型组,痛阈高于模型组;进一步采用ELISA检测血清中IL-1β水平显示蜂毒组血清IL-1β水平比模型组低。结论:蜂毒穴位注射足三里能减轻足跖肿胀,提高痛阈,同时抑制炎性细胞因子IL-1β的转录和表达,对AA大鼠有效。
