After reading the article "The Boundedness and Asymptotic Behavior of Solution of Differential System of Second-Order with Variable Coefficient" in "Applied Mathematics and Mechanics", Vol. 3, No. ...After reading the article "The Boundedness and Asymptotic Behavior of Solution of Differential System of Second-Order with Variable Coefficient" in "Applied Mathematics and Mechanics", Vol. 3, No. 4, 1982, we would like to put forward a few points to discuss with the author and the readers. Our opinions are presented as follows:展开更多
Rapid advances in Ribonucleic Acid sequencing(or RNA-seq)technology for analyzing entire transcriptomes of desired tissue samples,or even of single cells at scale,have revolutionized biology in the past decade.Increas...Rapid advances in Ribonucleic Acid sequencing(or RNA-seq)technology for analyzing entire transcriptomes of desired tissue samples,or even of single cells at scale,have revolutionized biology in the past decade.Increasing accessibility and falling costs are making it possible to address many problems in biology that were once considered intractable,including the study of various social behaviors.RNA-seq is opening new avenues to understand long-standing questions on the molecular basis of behavioral plasticity and individual variation in the expression of a behavior.As whole transcriptomes are examined,it has become possible to make unbiased discoveries of underlying mechanisms with little or no necessity to predict genes involved in advance.However,researchers need to be aware of technical limitations and have to make specific decisions when applying RNA-seq to study social behavior.Here,we provide a perspective on the applications of RNA-seq and experimental design considerations for behavioral scientists who are unfamiliar with the technology but are considering using it in their research.展开更多
文摘After reading the article "The Boundedness and Asymptotic Behavior of Solution of Differential System of Second-Order with Variable Coefficient" in "Applied Mathematics and Mechanics", Vol. 3, No. 4, 1982, we would like to put forward a few points to discuss with the author and the readers. Our opinions are presented as follows:
基金This work was supported by Yale-NUS College through grants R-607-265-225-121 and IG16-LR003.
文摘Rapid advances in Ribonucleic Acid sequencing(or RNA-seq)technology for analyzing entire transcriptomes of desired tissue samples,or even of single cells at scale,have revolutionized biology in the past decade.Increasing accessibility and falling costs are making it possible to address many problems in biology that were once considered intractable,including the study of various social behaviors.RNA-seq is opening new avenues to understand long-standing questions on the molecular basis of behavioral plasticity and individual variation in the expression of a behavior.As whole transcriptomes are examined,it has become possible to make unbiased discoveries of underlying mechanisms with little or no necessity to predict genes involved in advance.However,researchers need to be aware of technical limitations and have to make specific decisions when applying RNA-seq to study social behavior.Here,we provide a perspective on the applications of RNA-seq and experimental design considerations for behavioral scientists who are unfamiliar with the technology but are considering using it in their research.
