The aim of this study is to investigate the functional relationship between filamin, a known actin binding protein, and myosin and the effects of filamin on the interaction between myosin and actin. Methods.Ultra cent...The aim of this study is to investigate the functional relationship between filamin, a known actin binding protein, and myosin and the effects of filamin on the interaction between myosin and actin. Methods.Ultra centrifugation method was used to investigate the binding of filamin to both phosphorylated and unphosphorylated myosins. Mg ATPase activities of both phosphorylated and unphosphorylated myosins in the presence and absence of actin were measured to observe the effects resulted from filamin actin and filamin myosin interactions. Results. It was found that filamin is also a myosin binding protein. Filamin inhibited the actin activated Mg ATPase activity of phosphorylated myosin and stimulated Mg ATPase of phosphorylated myosin in the absence of actin; in addition, filamin stimulated Mg ATPase activity of unphosphorylated myosin in both the presence or absence of actin. Conclusion. The results suggest that the effects of filamin on the myosin Mg ATPase activities are bi directional, i.e., stimulatory via binding to myosin and inhibitory via binding to actin.展开更多
This paper presents the design of hierarchical communication network for traction power supply supervisory control and data acquisition (SCADA) system. Some key points, such as data transmission synchronization, datab...This paper presents the design of hierarchical communication network for traction power supply supervisory control and data acquisition (SCADA) system. Some key points, such as data transmission synchronization, database distribution, data transformation, and the network communication optimization at the supervisory level of the communication network are discussed. At the control level, based on the specificity of communication lines for electrified railway, a structure of two layer ring telecommunication network and the efficient bi directional polling scheme in software design are utilized. Then, an analysis for the scheme under normal and disturbance conditions is given. By means of the scheme, software switching over at a fault point of a channel is realized automatically.展开更多
文摘The aim of this study is to investigate the functional relationship between filamin, a known actin binding protein, and myosin and the effects of filamin on the interaction between myosin and actin. Methods.Ultra centrifugation method was used to investigate the binding of filamin to both phosphorylated and unphosphorylated myosins. Mg ATPase activities of both phosphorylated and unphosphorylated myosins in the presence and absence of actin were measured to observe the effects resulted from filamin actin and filamin myosin interactions. Results. It was found that filamin is also a myosin binding protein. Filamin inhibited the actin activated Mg ATPase activity of phosphorylated myosin and stimulated Mg ATPase of phosphorylated myosin in the absence of actin; in addition, filamin stimulated Mg ATPase activity of unphosphorylated myosin in both the presence or absence of actin. Conclusion. The results suggest that the effects of filamin on the myosin Mg ATPase activities are bi directional, i.e., stimulatory via binding to myosin and inhibitory via binding to actin.
文摘This paper presents the design of hierarchical communication network for traction power supply supervisory control and data acquisition (SCADA) system. Some key points, such as data transmission synchronization, database distribution, data transformation, and the network communication optimization at the supervisory level of the communication network are discussed. At the control level, based on the specificity of communication lines for electrified railway, a structure of two layer ring telecommunication network and the efficient bi directional polling scheme in software design are utilized. Then, an analysis for the scheme under normal and disturbance conditions is given. By means of the scheme, software switching over at a fault point of a channel is realized automatically.
