Sucrose nonfermenting-related protein kinase 1(SnRK1)is one of the critical serine/threonine protein kinases.It commonly mediates plant growth and development,cross-talks with metabolism processes and physiological re...Sucrose nonfermenting-related protein kinase 1(SnRK1)is one of the critical serine/threonine protein kinases.It commonly mediates plant growth and development,cross-talks with metabolism processes and physiological responses to biotic or abiotic stresses.It plays a key role in distributing carbohydrates and sugar signal transporting.In the present study,eight SnRK1 coding genes were identified in sorghum(Sorghum bicolor L.)via sequences alignment,with three forαsubunits(SnRK1α1 to SnRK1α3),three forβ(SnRK1β1 to SnRK1β3),and one for bothγ(SnRK1γ)andβγ(SnRK1βγ).These eight corresponding genes located on five chromosomes(Chr)of Chr1–3,Chr7,and Chr9 and presented collinearities to SnRK1s from maize and rice,exhibiting highly conserved domains within the same subunits from the three kinds of cereals.Expression results via qRT-PCR showed that different coding genes of SnRK1s in sorghum possessed similar expression patterns except for SnRK1α3 with a low expression level in grains and SnRK1β2 with a relatively high expression level in inflorescences.Results of subcellular localization in sorghum leaf protoplast showed that SnRK1α1/α2/α3/γmainly located on organelles,while the rest four of SnRK1β1/β2/β3/βγlocated on both membranes and some organelles.Besides,three combinations were discovered among eight SnRK1 subunits in sorghum through yeast two hybrid,includingα1-β2-βγ,α2-β3-γ,andα3-β3-γ.These results provide informative references for the following functional dissection of SnRK1 subunits in sorghum.展开更多
Plants overcome environmental stress by generating metabolic pathways.Thus,it is crucial to understand the physiological mechanisms of plant responses to changing environments.Ardisia crenata var.bicolor has an import...Plants overcome environmental stress by generating metabolic pathways.Thus,it is crucial to understand the physiological mechanisms of plant responses to changing environments.Ardisia crenata var.bicolor has an important ornamental and medicinal value.To reveal the impact of elevational gradient on the habitat soil and plant physiological attributes of this species,we collected root topsoil(0–20 cm)and subsoil(20–40 cm)samples and upper leaves at the initial blooming phase,in a survey of six elevations at 1,257 m,1,538 m,1,744 m,1,970 m,2,135 m,and 2,376 m,with 18 block plots,and 5sampling points at each site.Temperature decreases with an increase in elevation,and soil variables,and enzymatic activities fluctuated in both the topsoil and subsoil,with all of them increasing with elevation and decreasing with soil depth.Redundancy analysis was conducted to explore the correlation between the distribution of A.crenata var.bicolor along the elevational gradient and soil nutrients and enzyme activities,the soil properties were mainly affected by p H at low elevations,and governed by total phosphorus(TP)and available nitrogen(AN)at high elevations.The levels of chlorophyll,carbohydrates,and enzymatic activity except for anthocyanin in this species showed significant variation depending on physiological attributes evaluated at the different collection elevations.The decline in chlorophyll a and b may be associated with the adaptive response to avoid environmental stress,while its higher soluble sugar and protein contents play important roles in escaping adverse climatic conditions,and the increases in activities of antioxidant enzymes except peroxidase(POD)reflect this species’higher capacity for reactive oxygen scavenging(ROS)at high elevations.This study provides supporting evidence that elevation significantly affects the physiological attributes of A.crenata var.bicolor on Gaoligong Mountain,which is helpful for understanding plant adaptation strategies and the plasticity of plant physiological traits along the elevational gradients.展开更多
基金supported by the National Natural Science Foundation of China(32001607)the Fundamental Research Funds for the Central Universities of Southwest University,China(SWU118087)。
文摘Sucrose nonfermenting-related protein kinase 1(SnRK1)is one of the critical serine/threonine protein kinases.It commonly mediates plant growth and development,cross-talks with metabolism processes and physiological responses to biotic or abiotic stresses.It plays a key role in distributing carbohydrates and sugar signal transporting.In the present study,eight SnRK1 coding genes were identified in sorghum(Sorghum bicolor L.)via sequences alignment,with three forαsubunits(SnRK1α1 to SnRK1α3),three forβ(SnRK1β1 to SnRK1β3),and one for bothγ(SnRK1γ)andβγ(SnRK1βγ).These eight corresponding genes located on five chromosomes(Chr)of Chr1–3,Chr7,and Chr9 and presented collinearities to SnRK1s from maize and rice,exhibiting highly conserved domains within the same subunits from the three kinds of cereals.Expression results via qRT-PCR showed that different coding genes of SnRK1s in sorghum possessed similar expression patterns except for SnRK1α3 with a low expression level in grains and SnRK1β2 with a relatively high expression level in inflorescences.Results of subcellular localization in sorghum leaf protoplast showed that SnRK1α1/α2/α3/γmainly located on organelles,while the rest four of SnRK1β1/β2/β3/βγlocated on both membranes and some organelles.Besides,three combinations were discovered among eight SnRK1 subunits in sorghum through yeast two hybrid,includingα1-β2-βγ,α2-β3-γ,andα3-β3-γ.These results provide informative references for the following functional dissection of SnRK1 subunits in sorghum.
基金supported by the Doctoral Research Fund Project of Southwest Forestry University(CN)(Grant No.111806)。
文摘Plants overcome environmental stress by generating metabolic pathways.Thus,it is crucial to understand the physiological mechanisms of plant responses to changing environments.Ardisia crenata var.bicolor has an important ornamental and medicinal value.To reveal the impact of elevational gradient on the habitat soil and plant physiological attributes of this species,we collected root topsoil(0–20 cm)and subsoil(20–40 cm)samples and upper leaves at the initial blooming phase,in a survey of six elevations at 1,257 m,1,538 m,1,744 m,1,970 m,2,135 m,and 2,376 m,with 18 block plots,and 5sampling points at each site.Temperature decreases with an increase in elevation,and soil variables,and enzymatic activities fluctuated in both the topsoil and subsoil,with all of them increasing with elevation and decreasing with soil depth.Redundancy analysis was conducted to explore the correlation between the distribution of A.crenata var.bicolor along the elevational gradient and soil nutrients and enzyme activities,the soil properties were mainly affected by p H at low elevations,and governed by total phosphorus(TP)and available nitrogen(AN)at high elevations.The levels of chlorophyll,carbohydrates,and enzymatic activity except for anthocyanin in this species showed significant variation depending on physiological attributes evaluated at the different collection elevations.The decline in chlorophyll a and b may be associated with the adaptive response to avoid environmental stress,while its higher soluble sugar and protein contents play important roles in escaping adverse climatic conditions,and the increases in activities of antioxidant enzymes except peroxidase(POD)reflect this species’higher capacity for reactive oxygen scavenging(ROS)at high elevations.This study provides supporting evidence that elevation significantly affects the physiological attributes of A.crenata var.bicolor on Gaoligong Mountain,which is helpful for understanding plant adaptation strategies and the plasticity of plant physiological traits along the elevational gradients.
文摘花叶芋(Calalium bicolor),又名五彩芋,属天南星科,是一种美丽的观叶植物,原是通过块茎繁殖,但其休眠期长,增殖速度慢,因此,利用组培扩大繁殖,以满足市场需要。关于花叶芋的组培,已有不少报道,但对合适的培养基的筛选,分化的途径都还有研究的必要。为此,我们以花叶芋叶柄、叶片为材料进行了初步研究现结果如下:1.愈伤组织的诱导和培养基的改进:所用培养基中在 MS 附加2,4-D1ppm,KT 1ppm、
