Background and aims:Non-alcoholic fatty liver disease(NAFLD)is closely associated with gut microbiota and has become the most common chronic liver disease worldwide,but the relationship between specific strains and NA...Background and aims:Non-alcoholic fatty liver disease(NAFLD)is closely associated with gut microbiota and has become the most common chronic liver disease worldwide,but the relationship between specific strains and NAFLD has not been fully elucidated.We aimed to investigate whether Akkermansia muciniphila and Bifidobacterium bifidum could prevent NAFLD,the effects of their action alone or in combination,possible mechanisms,and modulation of the gut microbiota.Methods:Mice were fed with high-fat diets(HFD)for 20 weeks,in which experimental groups were pretreated with quadruple antibiotics and then given the corresponding bacterial solution or PBS.The expression of the glycolipid metabolism indicators,liver,and intestinal farnesol X receptors(FXR),and intestinal mucosal tight junction proteins were detected.We also analyzed the alterations of inflammatory and immune status and the gut microbiota of mice.Results:Both strains were able to attenuate mass gain(p<0.001),insulin resistance(p<0.001),and liver lipid deposition(p<0.001).They also reduced the levels of the pro-inflammatory factors(p<0.05)and the proportion of Th17(p<0.001),while elevating the proportion of Treg(p<0.01).Both strains activated hepatic FXR while suppressing intestinal FXR(p<0.05),and elevating tight junction protein expression(p<0.05).We also perceived changes in the gut microbiota and found both strains were able to synergize beneficial microbiota to function.Conclusions:Administration of A.muciniphila or B.bifidum alone or in combination was protective against HFD-induced NAFLD formation and could be used as alternative treatment strategy for NAFLD after further exploration.展开更多
目的探究双歧杆菌联合光疗对黄疸患儿血清胆红素水平的影响。方法采用横断面法,选取2020年10月—2023年6月江苏省启东市妇幼保健院接收的86例黄疸患儿为研究对象,利用统计学方法对其进行随机编号并分为对照组(43例)及观察组(43例)。对...目的探究双歧杆菌联合光疗对黄疸患儿血清胆红素水平的影响。方法采用横断面法,选取2020年10月—2023年6月江苏省启东市妇幼保健院接收的86例黄疸患儿为研究对象,利用统计学方法对其进行随机编号并分为对照组(43例)及观察组(43例)。对照组单纯使用蓝光治疗法,观察组予以双歧杆菌联合蓝光照射治疗。对比两组患儿血清胆红素水平、免疫功能及心功能。结果观察组总胆红素(123.12±26.47)μmol/L、间接胆红素(112.36±22.49)μmol/L、直接胆红素(8.56±2.17)μmol/L水平均低于对照组,差异有统计学意义(t=2.437、2.514、3.380,P均<0.05)。观察组T淋巴细胞(TLymphocytes,CD3+)、辅助T细胞(HelperTCells,CD4+)、CD4+/细胞毒性T细胞(Cytotoxic T Cells,CD8+)均高于对照组,且CD8+低于对照组,差异有统计学意义(P均<0.05)。观察组肌酸激酶、肌酸激酶同工酶MB型、乳酸脱氢酶、天冬氨酸氨基转移酶水平均低于对照组,差异有统计学意义(P均<0.05)。结论针对黄疸患儿采取蓝光照射治疗联合双歧杆菌治疗,有助于降低血清胆红素水平,并改善细胞免疫功能及心功能。展开更多
基金This study was supported by the Natural Science Foundation of Shanghai(20ZR1450100)the Characteristic Discipline of Shanghai Pudong New Area(PWYts2021-11)the Key Discipline of Shanghai Pudong Hospital(Zdxk2020-07).
文摘Background and aims:Non-alcoholic fatty liver disease(NAFLD)is closely associated with gut microbiota and has become the most common chronic liver disease worldwide,but the relationship between specific strains and NAFLD has not been fully elucidated.We aimed to investigate whether Akkermansia muciniphila and Bifidobacterium bifidum could prevent NAFLD,the effects of their action alone or in combination,possible mechanisms,and modulation of the gut microbiota.Methods:Mice were fed with high-fat diets(HFD)for 20 weeks,in which experimental groups were pretreated with quadruple antibiotics and then given the corresponding bacterial solution or PBS.The expression of the glycolipid metabolism indicators,liver,and intestinal farnesol X receptors(FXR),and intestinal mucosal tight junction proteins were detected.We also analyzed the alterations of inflammatory and immune status and the gut microbiota of mice.Results:Both strains were able to attenuate mass gain(p<0.001),insulin resistance(p<0.001),and liver lipid deposition(p<0.001).They also reduced the levels of the pro-inflammatory factors(p<0.05)and the proportion of Th17(p<0.001),while elevating the proportion of Treg(p<0.01).Both strains activated hepatic FXR while suppressing intestinal FXR(p<0.05),and elevating tight junction protein expression(p<0.05).We also perceived changes in the gut microbiota and found both strains were able to synergize beneficial microbiota to function.Conclusions:Administration of A.muciniphila or B.bifidum alone or in combination was protective against HFD-induced NAFLD formation and could be used as alternative treatment strategy for NAFLD after further exploration.
文摘目的探究双歧杆菌联合光疗对黄疸患儿血清胆红素水平的影响。方法采用横断面法,选取2020年10月—2023年6月江苏省启东市妇幼保健院接收的86例黄疸患儿为研究对象,利用统计学方法对其进行随机编号并分为对照组(43例)及观察组(43例)。对照组单纯使用蓝光治疗法,观察组予以双歧杆菌联合蓝光照射治疗。对比两组患儿血清胆红素水平、免疫功能及心功能。结果观察组总胆红素(123.12±26.47)μmol/L、间接胆红素(112.36±22.49)μmol/L、直接胆红素(8.56±2.17)μmol/L水平均低于对照组,差异有统计学意义(t=2.437、2.514、3.380,P均<0.05)。观察组T淋巴细胞(TLymphocytes,CD3+)、辅助T细胞(HelperTCells,CD4+)、CD4+/细胞毒性T细胞(Cytotoxic T Cells,CD8+)均高于对照组,且CD8+低于对照组,差异有统计学意义(P均<0.05)。观察组肌酸激酶、肌酸激酶同工酶MB型、乳酸脱氢酶、天冬氨酸氨基转移酶水平均低于对照组,差异有统计学意义(P均<0.05)。结论针对黄疸患儿采取蓝光照射治疗联合双歧杆菌治疗,有助于降低血清胆红素水平,并改善细胞免疫功能及心功能。
