Barrett's esophagus (BE) is characterized by intestinal metaplasia with the differentiated epithelium replaced by another type of epithelium morphologically similar to normal intestinal epithelium. The metaplasia ...Barrett's esophagus (BE) is characterized by intestinal metaplasia with the differentiated epithelium replaced by another type of epithelium morphologically similar to normal intestinal epithelium. The metaplasia is preceded by bile and acid reflux into the esophagus. BE is a premalignant condition associated with increased risk of esophageal cancer, especially esophageal adenocarcinoma. The Caudal-related homeodomain transcription factors Caudal-related homeodomain transcription factor CDX1 and CDX2 are expressed exclusively in the small and large intestine, playing important roles in proliferation and differentiation of intestinal epithelial cells. Ectopic expression of CDX1 and CDX2 occurs in BE. The apical sodium-dependent bile acid transporter (ASBT) is expressed primarily in terminal ileum where it is a key factor for intestinal reabsorption of bile salts. In addition to upregulation of CDX1 and CDX2, ASBT expression is up-regulated in BE. Furthermore, both CDX1/CDX2 and ASBT expressions are down-regulated in high-grade esophageal dysplasia. The alteration of the above-mentioned factors calls for attention: what is the relationship between CDXs and ASBT aberrant expression in BE? In this commentary, we discuss this issue on basis of the recent study done by Ma et al .展开更多
目的：建立高效液相色谱法测定人工养殖蟒蛇胆汁中牛磺胆酸钠含量的方法。方法：采用Phe-nomenex Luna C18色谱柱（250mm×4.60mm,5μm）;流动相为乙腈-0.15%磷酸氢二钠缓冲盐（pH=3.0）溶液,梯度洗脱;流速为1.0 mL.min-1;检测波长...目的：建立高效液相色谱法测定人工养殖蟒蛇胆汁中牛磺胆酸钠含量的方法。方法：采用Phe-nomenex Luna C18色谱柱（250mm×4.60mm,5μm）;流动相为乙腈-0.15%磷酸氢二钠缓冲盐（pH=3.0）溶液,梯度洗脱;流速为1.0 mL.min-1;检测波长为203nm;柱温为35℃。结果：牛磺胆酸钠在0.0051-0.0816mg.mL-1的浓度范围内线性关系良好,r=0.9997。平均回收率为102.1%,RSD=1.6%（n=6）。7批蟒蛇胆汁中牛磺胆酸钠的平均含量7.35mg.g-1。结论：该方法简便、可行,能准确测定人工养殖蟒蛇胆汁中牛磺胆酸钠的含量,为蟒蛇胆汁的质量控制提供理论依据。展开更多
文摘Barrett's esophagus (BE) is characterized by intestinal metaplasia with the differentiated epithelium replaced by another type of epithelium morphologically similar to normal intestinal epithelium. The metaplasia is preceded by bile and acid reflux into the esophagus. BE is a premalignant condition associated with increased risk of esophageal cancer, especially esophageal adenocarcinoma. The Caudal-related homeodomain transcription factors Caudal-related homeodomain transcription factor CDX1 and CDX2 are expressed exclusively in the small and large intestine, playing important roles in proliferation and differentiation of intestinal epithelial cells. Ectopic expression of CDX1 and CDX2 occurs in BE. The apical sodium-dependent bile acid transporter (ASBT) is expressed primarily in terminal ileum where it is a key factor for intestinal reabsorption of bile salts. In addition to upregulation of CDX1 and CDX2, ASBT expression is up-regulated in BE. Furthermore, both CDX1/CDX2 and ASBT expressions are down-regulated in high-grade esophageal dysplasia. The alteration of the above-mentioned factors calls for attention: what is the relationship between CDXs and ASBT aberrant expression in BE? In this commentary, we discuss this issue on basis of the recent study done by Ma et al .
文摘目的：建立高效液相色谱法测定人工养殖蟒蛇胆汁中牛磺胆酸钠含量的方法。方法：采用Phe-nomenex Luna C18色谱柱（250mm×4.60mm,5μm）;流动相为乙腈-0.15%磷酸氢二钠缓冲盐（pH=3.0）溶液,梯度洗脱;流速为1.0 mL.min-1;检测波长为203nm;柱温为35℃。结果：牛磺胆酸钠在0.0051-0.0816mg.mL-1的浓度范围内线性关系良好,r=0.9997。平均回收率为102.1%,RSD=1.6%（n=6）。7批蟒蛇胆汁中牛磺胆酸钠的平均含量7.35mg.g-1。结论：该方法简便、可行,能准确测定人工养殖蟒蛇胆汁中牛磺胆酸钠的含量,为蟒蛇胆汁的质量控制提供理论依据。
文摘采用化学法制备了白果壳水不溶性膳食纤维,产率为80.11%,其持水力和溶胀力分别为4.14 g/g和1.78 mL/g.测定了白果壳水不溶性膳食纤维对胆酸钠的吸附作用.白果壳水不溶性膳食纤维在pH6.0条件下对胆酸钠具有明显的吸附作用,其吸附能力随其用量的增大而增强,但到达吸附平衡的时间也相应增加.膳食纤维用量分别为1.0、2.0、3.0、4.0 g时,其最终吸附率分别为11.75%、29.3%、64.8%和76.0%,到达吸附平衡的时间分别为1.8、2.7、3.8、4.1 h.
