室内饲养地中海蟋蟀(Gryllus bimaculatus De Gccr)雄性成虫,解剖出侧体(CA)离休培养、测定其保幼激素Ⅲ(JH)的生物合成率。未交配和交配两组处理结果表明:成虫羽化后2天JH Ⅲ的生物合成率均达到最高峰,但交配组雄虫的生物合成率约是未...室内饲养地中海蟋蟀(Gryllus bimaculatus De Gccr)雄性成虫,解剖出侧体(CA)离休培养、测定其保幼激素Ⅲ(JH)的生物合成率。未交配和交配两组处理结果表明:成虫羽化后2天JH Ⅲ的生物合成率均达到最高峰,但交配组雄虫的生物合成率约是未交配组的2倍;羽化2天后,未交配雄成虫JH Ⅲ的生物合成率平稳下降,而交配过的雄成虫有一段时间JHⅢ生物合成率有起伏。成虫羽化后的4天内血淋巴中JHⅢ的滴定度与CA离体培养的JHⅢ的生物合成率相近。心侧体-咽侧体(CC-CA)-复合体与分离的脑一起培养,_羽化后2天JHⅢ生物合成率下降24％。8天下降45％;完整的脑-CC-CA-复合体培养,羽化后2天和8天,均下降95％。展开更多
The butter catfish,Ompok bimaculatus,belonging to the silurid family,is widely regarded as an important food fish for aquaculture diversification in India.Furthermore,the species poses a threat due to habitat degradat...The butter catfish,Ompok bimaculatus,belonging to the silurid family,is widely regarded as an important food fish for aquaculture diversification in India.Furthermore,the species poses a threat due to habitat degradation and other anthropogenic factors,and has been categorised as“near threatened”,therefore,its culture promotion is warranted.To establish a successful breeding programme,a precise understanding of the life history,and biology(reproductive/feeding)of the fish will assist hatchery managers and researchers.Studies in these areas have been undertaken by several groups over the years,but without much coherence,the data are still fragmented.Few attempts on the culture attributes of this catfish have been performed under both mono-and polyculture systems to bring about parallel promotion through aquaculture in a larger part of the subcontinent.Highlighting this,we have tried to analyse and present a consolidated account of the morphological characteristics,feeding habits,reproductive biology,early developmental ontogeny,and culture potentials of O.bimaculatus in India.This review was also prompted by the paucity of information on the cultural aspects of the species.Additionally,based on the reported studies,future directions and perspectives of the culture promotion of the species are proffered to ensure future research initiatives.展开更多
Suitable reference genes are one of the necessary conditions for obtaining reliable results by real-time fluorescence quantitative PCR(qPCR).In this study,the expression of the 10 common candidate reference genes(18s ...Suitable reference genes are one of the necessary conditions for obtaining reliable results by real-time fluorescence quantitative PCR(qPCR).In this study,the expression of the 10 common candidate reference genes(18s rRNA,rps27,cnbp,rpl7,ube2,hsp-at,gapdh,β-actin,rpl13a,1-ef1a)at different developmental stages of gonad and embryo of Takifugu bimaculatus were analyzed by qPCR.And the expression stability of these reference genes is analyzed by GeNorm,NormFinder and Bestkeeper softwares.The results showed that the expression stability of 1-ef1a was the highest(p<0.05)combined with the results of three analysis softwares,and the optimal number of reference genes was 3,which were 1-ef1a,hsp-at and rps27.Based on this result,we analyzed the expression of objective genes(foxl2 and dmrt1)at different developmental stages(early,medium-term and late)of gonads and embryos(eye vesicle stage,motility stage,heart anlage stage,retinal pigmentation stage,newly hatched larva)of T.bimaculatus by qPCR.Among which the highest expression level of foxl2 was in the middle stage of ovary(p<0.05),and dmrt1 was expressed in the early stage of testis with the highest level(p<0.05).The expression levels of foxl2 and dmrt1 in the early stages of embryonic development were low or even almost non-existent,and had a slight increase in the late embryonic development(newly hatched larva).The expression profile of objective genes are consistent with their roles in the development of gonads and embryos,which indicates that 1-ef1a,hsp-at and rps27 are the suitable reference gene combination for detecting the expression of objective genes by qPCR in gonads and embryos of T.bimaculatus.This research also provides the reliability data for screening the reference genes by qPCR in fish.展开更多
Salicylaldehyde O,O dimethyl phosphoramidothioic and their complexes of copper(Ⅱ),nicke(Ⅱ) and Zinc(Ⅱ) were synthesized and characterized by elemental analysis,electronic absorption spectrum,IR and molar conductivi...Salicylaldehyde O,O dimethyl phosphoramidothioic and their complexes of copper(Ⅱ),nicke(Ⅱ) and Zinc(Ⅱ) were synthesized and characterized by elemental analysis,electronic absorption spectrum,IR and molar conductivity.The schiffbase ligand was further ascertained by 1HNMR spectra.The composition coordination state and possible structures in these compounds were given.The tests of biological activity showed that synthesized complexes have scavenging effect on superoxide free radical similar SOD enzyme and these compounds are effective chemicals to kill tetranychus bimaculatus.展开更多
文摘The butter catfish,Ompok bimaculatus,belonging to the silurid family,is widely regarded as an important food fish for aquaculture diversification in India.Furthermore,the species poses a threat due to habitat degradation and other anthropogenic factors,and has been categorised as“near threatened”,therefore,its culture promotion is warranted.To establish a successful breeding programme,a precise understanding of the life history,and biology(reproductive/feeding)of the fish will assist hatchery managers and researchers.Studies in these areas have been undertaken by several groups over the years,but without much coherence,the data are still fragmented.Few attempts on the culture attributes of this catfish have been performed under both mono-and polyculture systems to bring about parallel promotion through aquaculture in a larger part of the subcontinent.Highlighting this,we have tried to analyse and present a consolidated account of the morphological characteristics,feeding habits,reproductive biology,early developmental ontogeny,and culture potentials of O.bimaculatus in India.This review was also prompted by the paucity of information on the cultural aspects of the species.Additionally,based on the reported studies,future directions and perspectives of the culture promotion of the species are proffered to ensure future research initiatives.
基金This work was supported by Natural Science Foundation of Fujian Province(2018J01451)Regional Demonstration of Marine Economy Innovative Development Project(16PZY002SF18)+3 种基金Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture(DF201905)Ornamental Aquarium Engineering Research Center in University of Fujian ProvinceUndergraduate Innovation Project of Fujian Province(201810390064,201910390047)College Students innovation and entrepreneurship training program of Jimei University(2020xj053,2020xj059)。
文摘Suitable reference genes are one of the necessary conditions for obtaining reliable results by real-time fluorescence quantitative PCR(qPCR).In this study,the expression of the 10 common candidate reference genes(18s rRNA,rps27,cnbp,rpl7,ube2,hsp-at,gapdh,β-actin,rpl13a,1-ef1a)at different developmental stages of gonad and embryo of Takifugu bimaculatus were analyzed by qPCR.And the expression stability of these reference genes is analyzed by GeNorm,NormFinder and Bestkeeper softwares.The results showed that the expression stability of 1-ef1a was the highest(p<0.05)combined with the results of three analysis softwares,and the optimal number of reference genes was 3,which were 1-ef1a,hsp-at and rps27.Based on this result,we analyzed the expression of objective genes(foxl2 and dmrt1)at different developmental stages(early,medium-term and late)of gonads and embryos(eye vesicle stage,motility stage,heart anlage stage,retinal pigmentation stage,newly hatched larva)of T.bimaculatus by qPCR.Among which the highest expression level of foxl2 was in the middle stage of ovary(p<0.05),and dmrt1 was expressed in the early stage of testis with the highest level(p<0.05).The expression levels of foxl2 and dmrt1 in the early stages of embryonic development were low or even almost non-existent,and had a slight increase in the late embryonic development(newly hatched larva).The expression profile of objective genes are consistent with their roles in the development of gonads and embryos,which indicates that 1-ef1a,hsp-at and rps27 are the suitable reference gene combination for detecting the expression of objective genes by qPCR in gonads and embryos of T.bimaculatus.This research also provides the reliability data for screening the reference genes by qPCR in fish.
文摘Salicylaldehyde O,O dimethyl phosphoramidothioic and their complexes of copper(Ⅱ),nicke(Ⅱ) and Zinc(Ⅱ) were synthesized and characterized by elemental analysis,electronic absorption spectrum,IR and molar conductivity.The schiffbase ligand was further ascertained by 1HNMR spectra.The composition coordination state and possible structures in these compounds were given.The tests of biological activity showed that synthesized complexes have scavenging effect on superoxide free radical similar SOD enzyme and these compounds are effective chemicals to kill tetranychus bimaculatus.
