Design and Simulation of Flow Field for Bone Tissue Engineering Sca old Based on Triply Periodic Minimal Surface

A novel method was proposed to design the structure of a bone tissue engineering scafold based on triply periodic minimal surface.In this method,reverse engineering software was used to reconstruct the surface from point cloud data.This method overcomes the limitations of commercially available software packages that prevent them from generating models with complex surfaces used for bone tissue engineering scafolds.Additionally,the fluid feld of the scafolds was simulated through a numerical method based on fnite volume and the cell proliferation performance was evaluated via an in vitro experiment.The cell proliferation and the mass flow evaluated in a bioreactor further verifed the flow feld simulated using computational fluid dynamics.The result of this study illustrates that the pressure value drops rapidly from 0.103 Pa to 0.011 Pa in the y-axis direction and the mass flow is unevenly distributed in the outlets.The mass flow in the side outlets is observed to be approximately 24.3 times higher thanthe bottom.Importantly,although the mean value of wall shear stress is signifcantly more than 0.05 Pa,there is stil a large area with a suitable shear stress below 0.05 Pa where most cells can proliferate well.The result shows that th inlet velocity 0.0075 m/s is suitable for cell proliferation in the scafold.This study provides an insight into the design analysis,and in vitro experiment of a bone tissue engineering scafold.

Preliminary Engineering Design of Toroidal Field Magnet System for Superconducting Tokamak HT-7U

HT7U is a large fusion experimental device. It will be built in the Institute of Plasma Physics of Chinese Academy of Sciences. The mission of HT-7U is to develop the scientific basis for a continuously operating tokama-k fusion reactor. This paper describes only a toroidal field (TF) superconducting magnet system of HT7U. In this paper, design criteria of conductor and stability analysis, coil winding and support structure design of magnet system, mechanical calculation and stress analysis, heat load evaluation are given.

Evaluation of genetic response of mesenchymal stem cells to nanosecond pulsed electric fields by whole transcriptome sequencing

BACKGROUND Mesenchymal stem cells(MSCs)modulated by various exogenous signals have been applied extensively in regenerative medicine research.Notably,nanosecond pulsed electric fields(nsPEFs),characterized by short duration and high strength,significantly influence cell phenotypes and regulate MSCs differentiation via multiple pathways.Consequently,we used transcriptomics to study changes in messenger RNA(mRNA),long noncoding RNA(lncRNA),microRNA(miRNA),and circular RNA expression during nsPEFs application.AIM To explore gene expression profiles and potential transcriptional regulatory mechanisms in MSCs pretreated with nsPEFs.METHODS The impact of nsPEFs on the MSCs transcriptome was investigated through whole transcriptome sequencing.MSCs were pretreated with 5-pulse nsPEFs(100 ns at 10 kV/cm,1 Hz),followed by total RNA isolation.Each transcript was normalized by fragments per kilobase per million.Fold change and difference significance were applied to screen the differentially expressed genes(DEGs).Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to elucidate gene functions,complemented by quantitative polymerase chain reaction verification.RESULTS In total,263 DEGs were discovered,with 92 upregulated and 171 downregulated.DEGs were predominantly enriched in epithelial cell proliferation,osteoblast differentiation,mesenchymal cell differentiation,nuclear division,and wound healing.Regarding cellular components,DEGs are primarily involved in condensed chromosome,chromosomal region,actin cytoskeleton,and kinetochore.From aspect of molecular functions,DEGs are mainly involved in glycosaminoglycan binding,integrin binding,nuclear steroid receptor activity,cytoskeletal motor activity,and steroid binding.Quantitative real-time polymerase chain reaction confirmed targeted transcript regulation.CONCLUSION Our systematic investigation of the wide-ranging transcriptional pattern modulated by nsPEFs revealed the differential expression of 263 mRNAs,2 miRNAs,and 65 lncRNAs.Our study demonstrates that nsPEFs may affect stem cells through several signaling pathways,which are involved in vesicular transport,calcium ion transport,cytoskeleton,and cell differentiation.

Genetic Engineering of Field, Industrial and Pharmaceutical Crops

Ability to modify plants at the genomic level by advanced molecular technology has enhanced the scope of improvements in plant traits attempted earlier through conventional breeding methods. Techniques such as genetic transformation have opened new vistas whereby functional genes, not commonly present in a particular species can be added from other species. The traits incorporated into the genetically engineered plants in the beginning were confined to those governed by dominant genes, e.g. insecticide resistance and herbicide tolerance but advancements with time now also permit the transfer of complexly inherited traits such as drought and cold tolerance. Transgenic technology is also useful in understanding gene expression and metabolic pathways which can then be used to harness the full genomic potential of the plant. This review presents a narrative on development of transgenics and their use for the improvement of field, industrial and pharmaceuticals crops. In addition, discussions are made on current status on genetically modified crops, hurdles to genetic engineering, overcoming strategies and future scope.

Consideration of Postgraduate Training under the Cross Field of Medical Information and Engineering

The slogan of“Combination of medicine and engineering”proposed in“Made in China 2025”has aroused great attention to higher engineering education.However,it is a difficult problem and challenge for schools and educators to effectively adapt to the economic and social development,train new medical and engineering talents,and explore and cultivate new subject growth points.In order to deal with engineering change and a new round of scientific and technological revolution,facing the challenge of new engineering construction.This paper will combine the reality and characteristics of colleges and universities,analyze the problems existing in the current medical engineering cross-graduate training,and put forward countermeasures and suggestions to promote the progress and development of science and technology in colleges and universities.

Study on Ground Engineering and Management of Carbonate Oil Field A under Rolling Development Mode

Carbonate rock has the characteristics of complicated accumulation rules,large-scale development,high yield but unstable production.Therefore,the management and control of surface engineering projects of carbonate rock oil and gas reservoirs faces huge difficulties and challenges.The construction of surface engineering should conform to the principle of integrated underground and ground construction and adapt to the oilfield development model.This paper takes the newly added area A of the carbonated oil field as an example to study the ground engineering under the rolling development mode and aims to provide the constructive ideas for the surface engineering under rolling development mode.The overall regional process design adheres to the design concept of"environmental protection,efficiency,and innovation",strictly follows the design specifications,and combines reservoir engineering and oil production engineering programs,oil and gas physical properties and chemical composition,product programs,ground natural conditions,etc.According to the technical and economic analysis and comparison of area A,this paper has worked out a suitable surface engineering construction,pipeline network layout and oil and gas gathering and transportation plan for area A.Some auxiliary management recommendations are also proposed in this paper,like sand prevention management and HSE management for carbonate reservoirs.

Built-in electric field induced S-scheme g-C_(3)N_(4)homojunction for efficient photocatalytic hydrogen evolution:Interfacial engineering and morphology control

S-scheme possesses superior redox capabilities compared with the II-scheme,providing an effective method to solve the innate defects of g-C_(3)N_(4)(CN).In this study,S-doped g-C_(3)N_(4)/g-C_(3)N_(4)(SCN-tm/CN)S-scheme homojunction was constructed by rationally integrating morphology control with interfacial engineering to enhance the photocatalytic hydrogen evolution performance.In-situ Kelvin probe force microscopy(KPFM)confirms the transport of photo-generated electrons from CN to SCN.Density functional theory(DFT)calculations reveal that the generation of a built-in electric field between SCN and CN enables the carrier separation to be more efficient and effective.Femtosecond transient absorption spectrum(fs-TAS)indicates prolonged lifetimes of SCN-tm/CN_(3)(τ1:9.7,τ2:110,andτ3:1343.5 ps)in comparison to those of CN(τ1:4.86,τ2:55.2,andτ3:927 ps),signifying that the construction of homojunction promotes the separation and transport of electron hole pairs,thus favoring the photocatalytic process.Under visible light irradiation,the optimized SCN-tm/CN_(3)exhibits excellent photocatalytic activity with the hydrogen evolution rate of 5407.3μmol·g^(−1)·h^(−1),which is 20.4 times higher than that of CN(265.7μmol·g^(−1)·h^(−1)).Moreover,the homojunction also displays an apparent quantum efficiency of 26.8%at 435 nm as well as ultra-long and ultra-stable cycle ability.This work offers a new strategy to construct highly efficient photocatalysts based on the metal-free conjugated polymeric CN for realizing solar energy conversion.

Toward tunable shape memory effect of NiTi alloy by grain size engineering:A phase field study

The inelastic deformations of shape memory alloys(SMAs)always show poor controllability due to the avalanche-like martensite transformation,and the effective control for the deformation of precision de-vices has been not yet mature.In this work,the phase field method was used to investigate the shape memory effects(SMEs)of NiTi SMAs undergoing grain size(GS)engineering,to obtain tunable one-way and stress-assisted two-way SMEs(OWSME and SATWSME).The OWSME and SATWSME of the systems with various gradient-nanograin structures and bimodal grain structure,as well as that with geometric gradients were simulated.The simulated results indicate that due to the GS dependences of martensite transformation and reorientation,the occurrence and expansion of martensite reorientation,martensite transformation and its reverse can be efficaciously controlled via the GS engineering.When combining the GS engineering and geometric gradient design,since the effects of GS and stress gradient can be su-perimposed or competing,and the responses of martensite reorientation,martensite transformation and its reverse to this are different,the OWSME and SATWSME of the geometrically graded systems with various nanograin structures can exhibit different improvements in controllability.In short,the reorienta-tion hardening modulus during OWSME is increased and the transformation temperature window during SATWSME is widened by GS engineering,indicating the improved controllability of SMEs.The optimal GS engineering schemes revealed in this work provide the basic reference and guidance for designing tun-able SMEs and producing NiTi-based driving devices catering to desired functional performance in various engineering fields.

An improved multidirectional velocity model for micro-seismic monitoring in rock engineering

An improved multidirectional velocity model was proposed for more accurately locating micro-seismic events in rock engineering. It was assumed that the stress wave propagation velocities from a micro-seismic source to three nearest monitoring sensors in a sensor's array arrangement were the same. Since the defined objective function does not require pre-measurement of the stress wave propagation velocity in the field, errors from the velocity measurement can be avoided in comparison to three traditional velocity models. By analyzing 24 different cases, the proposed multidirectional velocity model iterated by the Simplex method is found to be the best option no matter the source is within the region of the sensor's array or not. The proposed model and the adopted iterative algorithm are verified by field data and it is concluded that it can significantly reduce the error of the estimated source location.

Continuous generation of lattice oxygen via redox engineering for boosting toluene degradation performances

Excellent performances promoted by lattice oxygen have attracted wide attention for catalytic degradation of volatile organic compounds(VOCs).However,how to control the continuous regeneration of lattice oxygen from the support is seldom reported.In this study,we selected sepiolite supported manganese-cobalt oxides(Co_(x)Mn_(100-x)O_(y))as model catalysts by tuning Co/(Co+Mn)mass ratio(x=3%,10%,15%,and 20%)to enhance toluene degradation efficiency,owing to lattice oxygen regeneration by redox cycle existing at the interface and Mn species with high valence state,initiated by cobalt catalytic performance under the role of crystal field stability phase.The results of activity test show that the sepiolite-Co_(15)Mn_(85)O_(y)catalyst exhibit outperformances at 193℃with 10,000 h^(-1)GHSV.In addition,the catalyst existed at the bottom of the"volcano"curve correlated T_(50)or T_(90)with Co/(Co+Mn)weight ratio is sepiolite-Co_(15)Mn_(85)O_(y),conforming its outperformance.Further characterized by investigating active sites structural and electronic properties,the essential of superior catalytic activity is attributed to the grands of lattice oxygen continuous formation resulted from redox engineering based on the high atomic ratio of surface lattice oxygen with continuous refilled from the support and that of Mn^(4+)/Mn^(3+)cycle initiated by cobalt catalytic behaviors.All in all,redox engineering,not only promotes grands of active species reversible regeneration,but supplies an alternative catalyst design strategy towards the terrific efficiency-to-cost ratio performance.

Nanoscale strain engineering of graphene and graphene-based devices

Structural distortions in nano-materials can induce dramatic changes in their electronic properties. This situation is well manifested in graphene, a two-dimensional honeycomb structure of carbon atoms with only one atomic layer thickness. In particular, strained graphene can result in both charging effects and pseudo-magnetic fields, so that controlled strain on a perfect graphene lattice can be tailored to yield desirable electronic properties. Here, we describe the theoretical foundation for strain-engineering of the electronic properties of graphene, and then provide experimental evidence for strain-induced pseudo-magnetic fields and charging effects in monolayer graphene. We further demonstrate the feasibility of nano-scale strain engineering for graphene-based devices by means of theoretical simulations and nano-fabrication technology.

Optimally arranged TiO_(2)@MoS_(2) heterostructures with effectively induced built-in electric field for high-performance lithium-sulfur batteries

To overcome the serious technological issues affecting lithium-sulfur(Li-S) batteries,such as sluggish sulfur redox kinetics and the detrimental shuttle effect,heterostructure engineering has been investigated as a strategy to effectively capture soluble lithium polysulfide intermediates and promote their conversion reaction by integrating highly polar metal oxides with catalytically active metals sulfides.However,to fully exploit the outstanding properties of heterostructure-based composites,their detailed structure and interfacial contacts should be designed rationally.Herein,optimally arranged TiO_(2)and MoS_(2)-based heterostructures(TiO_(2)@MoS_(2)) are fabricated on carbon cloth as a multifunctional interlayer to efficiently trap polysulfide intermediates and accelerate their redox kinetics.Owing to the synergistic effects between TiO_(2)and MoS_(2)and the uniform heterointerface distribution that induces the ideally oriented built-in electric field,Li-S batteries with TiO_(2)@MoS_(2)interlayers exhibit high rate capability(601 mA h g^(-1)at 5 C),good cycling stability(capacity-fade rate of 0.067% per cycle over 500 cycles at2 C),and satisfactory areal capacity(5.2 mA h cm^(-2)) under an increased sulfur loading of 5.2 mg cm^(-2).Moreover,by comparing with a MoS_(2)@TiO_(2)interlayer composed of reversely arranged heterostructures,the effect of the built-in electric field’s direction on the electrocatalytic reactions of polysulfide intermediates is thoroughly investigated for the first time.The superior electrocatalytic activities of the rationally arranged TiO_(2)@MoS_(2)interlayer demonstrate the importance of optimizing the built-in electric field of heterostructures for producing high-performance Li-S batteries.

Application of Enzyme Engineering in Pharmacy

Enzyme engineering is an important part of modern biotechnology.Due to its high reaction specificity,high efficiency,mild reaction conditions,and low pollution,it is also an important method widely used in the pharmaceutical field.The application of enzymes in medicine is diverse,such as:diagnosis,prevention and treatment of diseases with enzymes,manufacture of various drugs with enzymes,etc.,mainly through manual operations,to obtain enzymes required by the pharmaceutical industry,and through various means Enzymes perform their catalytic functions.This article mainly introduces the application of enzyme engineering in the pharmaceutical field,and also prospects the development trend of enzyme engineering in the pharmaceutical field.

Numerical Simulation of Airflow Field and Structure Improvement in Engine Compartment of Armored Vehicles with Electric Transmission

When the mechanical drive is changed into the electric transmission,the cooling system of the engine compartment should be altered to meet the new requirement for the increasing in equipment such as electric apparatus.In order to predict and analyze the rationality of cooling system in the virtual engine compartment,the numerical simulation of airflow fields in the engine compartment by using computational fluid dynamics(CFD) technique is necessary.An armored vehicle with electric transmission in the research is taken as the research object.The physical model and mathematical model for the computation of 3D air flow and heat transfer in the engine compartment of an armored vehicle with electric transmission is established.Turbulent flow in the compartment is described by using the standard k-ε two-equation turbulence model.The temperature and velocity fields of 3D air flow in the engine compartment are numerically simulated and analyzed based on different fan's flux.A theoretical basis for determination of the fan's flux is given by the simulation results.The positions of the air-vent shutter are analyzed.The simulation results show that the different positions of the air-vent shutter can lead to different cooling efficiencies.

Experiment Study on the Exhaust-Gas Heat Exchanger for Small and Medium-Sized Marine Diesel Engine

This paper aims to design a special exchanger to recover the exhaust gas heat of marine diesel engines used in small and medium-sized fishing vessels,which can then be used to heat water up to 55°C–85°C for membrane desalination devices to produce fresh water.A new exhaust-gas heat exchanger of fins and tube,with a reinforced heat transfer tube section,unequal spacing fins,a mixing zone between the fin groups and four routes tube bundle,was designed.Numerical simulations were also used to provide reference information for structural design.Experiments were carried out for exhaust gas waste heat recovery from a marine diesel engine in an engine test bench utilizing the heat exchanger.The experimental results show that the difference between heat absorption by water and heat reduction of exhaust gas is less than 6.5%.After the water flow rate was adjusted,the exhaust gas waste heat recovery efficiency was higher than 70%,and the exhaust-gas heat exchanger’s outlet water temperature was 55°C–85°C at different engine loads.This means that the heat recovery from the exhaust gas of a marine diesel engine meets the requirement to drive a membrane desalination device to produce fresh water for fishers working in small and medium-sized fishing vessels.

职业教育现场工程师培养:何以可为与何以作为

面向先进制造业等重点领域的数字化、智能化职业场景,实施职业教育现场工程师专项培养,既是增强职业教育适应性、推进产业转型升级所需,亦是优化人才供给结构赋能制造强国建设、彰显职业教育类型特征所向,有其自身“可为”的逻辑遵循。在实践中,应当深化产教融合,打造基于专业群的现场工程师学院,推进数字赋能、创新基于学徒制的培养模式,实施专业认证、构建“专业认证+职业资格证书”的培养标准,强化关键能力、培育教师教学创新团队,加强专题研究、夯实现场工程师教育理论与实践根基,以实际“作为”贡献职教智慧与工程力量。

高地应力软岩隧道仰拱结构受力特性与参数优化

为探索高地应力软岩隧道施工期间仰拱结构受力特性与合理支护参数,以陕西省宝汉高速公路连城山隧道工程为依托,针对仰拱施工病害及其主要影响因子,采用现场试验与数值模拟相结合的方法,探讨了不同影响因素下的仰拱结构变形与力学响应,分析了仰拱结构变形与受力对不同影响因素的敏感性,基于仰拱结构病害特征及数值模拟结果,对仰拱半径(深度)、仰拱厚度等支护参数进行了优化,并进行了工程验证。结果表明:连城山隧道施工期间仰拱二次衬砌最大接触压力达1.034 MPa,混凝土最大应力超过其抗压强度(30 MPa),出现了仰拱回填隆起、开裂等病害,仰拱设计支护参数难以保障结构稳定;地应力水平和基底围岩劣化对仰拱结构变形与受力状态均有显著影响,其中基底软化深度的影响最为突出,地应力水平次之;减小仰拱半径、增大仰拱厚度、增大仰拱钢筋直径、减小仰拱钢筋间距均能显著抑制仰拱变形,而提高混凝土强度等级对控制仰拱变形效果不明显,且经济性较差;优化后的仰拱受力得以明显改善,有效控制了仰拱结构病害,避免了频繁拆换仰拱,保障了隧道结构安全稳定。研究成果可为类似软岩隧道仰拱变形控制及病害防治提供重要借鉴。

大凉山背斜核部区隧道初支大变形机理与控制

针对大凉山2号公路隧道穿越高地应力背斜核心区域,隧道易发生初支大变形、坍塌、钢架扭曲等问题,通过现场监控量测、围岩岩性实验、理论分析、数值模拟分析和围岩接触压力监测等综合分析,研究了大凉山2号隧道背斜核部大变形成因机理。研究表明:背斜核部玄武岩单轴抗压强度为23.31 MPa,凝灰质泥岩遇水具有一定膨胀性,给围岩变形创造了条件。由于岩层岩性不同,开挖后洞周玄武岩呈独立受弯剪梁状态。相对无水条件下,受地下水补给影响,围岩塑性区面积增加,拱腰部位较为明显。加固前,拱顶沉降量最大达470 mm,拱腰最大收敛值为844 mm,围岩接触压力为523.47 kPa。采用双层小导管+中台阶临时支撑+边墙临时支撑的“主动适应+强支撑”的综合处置方式,隧道拱顶变形减少87.6%,水平收敛变形减少95%,围岩接触压力最大值降至128 kPa,隧道变形得到有效控制。

科教融汇理念下现场工程师培养的内涵意蕴、基本遵循与实践路径

科教融汇既是科技创新发展的崭新要求,也是推动职业教育适应产业需求的有效途径。为应对产业数字化转型下的人才素质需求变化,国家围绕产业高端和高端产业提出“职业教育现场工程师专项培养计划”,旨在培养具有一线生产运作技能,并能优化生产流程的高素质技术技能型人才。为实现这一类型人才培养,政府主管部门应统筹多方科教资源,辐射现场工程师培养框架建设;职业院校应积极主导并搭建多方协同育人体系,共建科教融汇平台资源,落实现场工程师技能孵化与输出;同时吸收社会力量有效补位,完善和深化培训课程体系适应性建设,赋能现场工程师可持续发展。

冻融条件下路基温度场和湿度场分布式感知试验

搭建土体冻融多物理场感知室内试验装置,布设传统点式传感器和分布式光纤,对土体冻融过程中路基温度场、湿度场和变形情况开展试验监测,探究单端冻结、双端融化条件下土体温度场和湿度场的分布式感知特征,验证主动加热分布式光纤技术的监测效果。试验结果表明,-15℃单端冻结条件下,土体冻结过程按降温速率分为快速冷却、逐步降温、稳定平衡三阶段,冻结锋面逐渐下移,最大冻深约占土柱高度的35%。孔隙水受冻胀迁移力影响向冻结锋面移动,引起冻融前后土体湿度场重分布,并导致土体冻胀变形,变形量为土体最大冻深的7.8%~10.9%。分布式光纤可准确得到冻融过程中土体温度场、湿度场变化特征,识别土体内冻结区域范围,温度、湿度监测拟合优度R2分别达0.98和0.94。