When nanoparticles(NPs)enter a physiological environment,they tend to adsorb proteins to form a so-called corona.A comprehensive understanding of the effect of protein corona on NPs’toxicity is required.Our previous ...When nanoparticles(NPs)enter a physiological environment,they tend to adsorb proteins to form a so-called corona.A comprehensive understanding of the effect of protein corona on NPs’toxicity is required.Our previous study indicated that silica nanoparticles(SiO_(2)NPs)exposure with different routes resulted in distinct brain damage;however,an exact molecular mechanism of protein corona on the regulation of SiO_(2)NPsinduced damages needs further investigation.SiO_(2)NPs exposure via intravenous injection may encounter a protein-rich bio-matrix,which drives the adsorption of serum protein on their surface to form a stable SiO_(2)NPs@serum complex.On the contrary,SiO_(2)NPs exposure via intranasal instillation remained their original feature,due to a protein infertile environment of cerebrospinal fluid.Apparently,surface adsorption of proteinaceous substances altered inherent toxic behavior of SiO_(2)NPs.In addition,glycogen synthase kinase 3 beta(GSK3β)phosphorylate was found at different residues,which play an essential role in orchestrating apoptosis and autophagy threshold.Route-dependent corona formation determined GSK3βphosphorylation status and ultimately the toxic behavior of SiO_(2)NPs.This work presented the evidence of biocorona on the regulation of SiO_(2)NPs-induced toxicity,which can be used to guide risk assessment of environmental NPs.展开更多
基金supported by National Natural Science Foundation of China(22176206,21976145,22174116,and 21974110).Notes。
文摘When nanoparticles(NPs)enter a physiological environment,they tend to adsorb proteins to form a so-called corona.A comprehensive understanding of the effect of protein corona on NPs’toxicity is required.Our previous study indicated that silica nanoparticles(SiO_(2)NPs)exposure with different routes resulted in distinct brain damage;however,an exact molecular mechanism of protein corona on the regulation of SiO_(2)NPsinduced damages needs further investigation.SiO_(2)NPs exposure via intravenous injection may encounter a protein-rich bio-matrix,which drives the adsorption of serum protein on their surface to form a stable SiO_(2)NPs@serum complex.On the contrary,SiO_(2)NPs exposure via intranasal instillation remained their original feature,due to a protein infertile environment of cerebrospinal fluid.Apparently,surface adsorption of proteinaceous substances altered inherent toxic behavior of SiO_(2)NPs.In addition,glycogen synthase kinase 3 beta(GSK3β)phosphorylate was found at different residues,which play an essential role in orchestrating apoptosis and autophagy threshold.Route-dependent corona formation determined GSK3βphosphorylation status and ultimately the toxic behavior of SiO_(2)NPs.This work presented the evidence of biocorona on the regulation of SiO_(2)NPs-induced toxicity,which can be used to guide risk assessment of environmental NPs.
