The therapeutic replacement of diseased tubular tissue is hindered by the availability and suitability of current donor, autologous and synthetically derived protheses. Artificially created, tissue engineered, constru...The therapeutic replacement of diseased tubular tissue is hindered by the availability and suitability of current donor, autologous and synthetically derived protheses. Artificially created, tissue engineered, constructs have the potential to alleviate these concerns with reduced autoimmune response, high anatomical accuracy, long-term patency and growth potential. The advent of 3D bioprinting technology has further supplemented the technological toolbox, opening up new biofabrication research opportunities and expanding the therapeutic potential of the field. In this review, we highlight the challenges facing those seeking to create artificial tubular tissue with its associated complex macro- and microscopic architecture. Current biofabrication approaches, including 3D printing techniques, are reviewed and future directions suggested.展开更多
Cell-laden cardiac patches have recently been emerging to renew cellular sources for myocardial infarction(MI,commonly know as a heart attack)repair.However,the fabrication of cell-laden patches with porous structure ...Cell-laden cardiac patches have recently been emerging to renew cellular sources for myocardial infarction(MI,commonly know as a heart attack)repair.However,the fabrication of cell-laden patches with porous structure remains challenging due to the limitations of currently available hydrogels and existing processing techniques.The present study utilized a bioprinting technique to fabricate hydrogel patches and characterize them in terms of printability,mechanical and biological properties.Cell-laden hydrogel(or bio-ink)was formulated from alginate dialdehyde(ADA)and gelatin(GEL)to improve the printability,degradability as well as bioactivity.Five groups of hydrogel compositions were designed to investigate the influence of the oxidation degree of ADA and hydrogels concentration on the properties of printed scaffolds.ADA-GEL hydrogels have generally shown favorable for living cells(EA.hy926 cells and hybrid human umbilical vein endothelial cell line).The hydrogel with an oxidation degree of 10%and a concentration ratio of 70/30(or 10%ADA70-GEL30)demonstrated the best printability among the groups examined.Formulated hydrogels were also bioprinted with the living cells(EA.hy926),and the scaffolds printed were then subject to the cell culture for 7 days.Our results illustrate that the scaffolds bioprinted from 10%ADA70–GEL30 hydrogels had the best homogenous cell distribution and also the highest cell viability.Taken together,in the present study we synthesized a newly formulated bio-ink from ADA and GEL and for the fist time,used them to bioprint cardiac patches,which have the potential to be used in MI repair.展开更多
Volumetric muscle loss(VML)is associated with a severe loss of muscle tissue that overwhelms the regenerative potential of skeletal muscles.Tissue engineering has shown promise for the treatment of VML injuries,as evi...Volumetric muscle loss(VML)is associated with a severe loss of muscle tissue that overwhelms the regenerative potential of skeletal muscles.Tissue engineering has shown promise for the treatment of VML injuries,as evidenced by various preclinical trials.The present study describes the fabrication of a cell-laden GelMa muscle construct using an in situ crosslinking(ISC)strategy to improve muscle functionality.To obtain optimal biophysical properties of the muscle construct,two UV exposure sources,UV exposure dose,and wall shear stress were evaluated using C2C12 myoblasts.Additionally,the ISC system showed a significantly higher degree of uniaxial alignment and myogenesis compared to the conventional crosslinking strategy(post-crosslinking).To evaluate the in vivo regenerative potential,muscle constructs laden with human adipose stem cells were used.The VML defect group implanted with the bio-printed muscle construct showed significant restoration of functionality and muscular volume.The data presented in this study suggest that stem cell-based therapies combined with the modified bioprinting process could potentially be effective against VML injuries.展开更多
基金We acknowledge the funding support from UK Engineering and Physical Sciences Research Council (EPSRC) on the Doctoral Prize Fellowship (Grant No. EP/N509760/1) for IH and the EngD studentship (Grant No. EP/L015595/1) for JL. JZS is funded by Overseas Scholarship Council and Ministry of Education in China. We also acknowledge the funding support from China-UK Research and Innovation Partnership Fund: Newton Fund Ph.D. placement programme. We thank the National Natural Science Foundation of China (No. 21534007), and the Beijing Municipal Science & Technology Commission for their financial support.
文摘The therapeutic replacement of diseased tubular tissue is hindered by the availability and suitability of current donor, autologous and synthetically derived protheses. Artificially created, tissue engineered, constructs have the potential to alleviate these concerns with reduced autoimmune response, high anatomical accuracy, long-term patency and growth potential. The advent of 3D bioprinting technology has further supplemented the technological toolbox, opening up new biofabrication research opportunities and expanding the therapeutic potential of the field. In this review, we highlight the challenges facing those seeking to create artificial tubular tissue with its associated complex macro- and microscopic architecture. Current biofabrication approaches, including 3D printing techniques, are reviewed and future directions suggested.
文摘Cell-laden cardiac patches have recently been emerging to renew cellular sources for myocardial infarction(MI,commonly know as a heart attack)repair.However,the fabrication of cell-laden patches with porous structure remains challenging due to the limitations of currently available hydrogels and existing processing techniques.The present study utilized a bioprinting technique to fabricate hydrogel patches and characterize them in terms of printability,mechanical and biological properties.Cell-laden hydrogel(or bio-ink)was formulated from alginate dialdehyde(ADA)and gelatin(GEL)to improve the printability,degradability as well as bioactivity.Five groups of hydrogel compositions were designed to investigate the influence of the oxidation degree of ADA and hydrogels concentration on the properties of printed scaffolds.ADA-GEL hydrogels have generally shown favorable for living cells(EA.hy926 cells and hybrid human umbilical vein endothelial cell line).The hydrogel with an oxidation degree of 10%and a concentration ratio of 70/30(or 10%ADA70-GEL30)demonstrated the best printability among the groups examined.Formulated hydrogels were also bioprinted with the living cells(EA.hy926),and the scaffolds printed were then subject to the cell culture for 7 days.Our results illustrate that the scaffolds bioprinted from 10%ADA70–GEL30 hydrogels had the best homogenous cell distribution and also the highest cell viability.Taken together,in the present study we synthesized a newly formulated bio-ink from ADA and GEL and for the fist time,used them to bioprint cardiac patches,which have the potential to be used in MI repair.
基金supported by a grant from the National Research Foundation of Korea funded by the Ministry of education,Science,and Technology(MEST)(Grant NRF-2018R1A2B2005263)supported by the National Research Foundation of Korea(NRF)Grant funded by the Ministry of Science and ICT for Bioinspired Innovation Technology Development Project(NRF-2018M3C1B7021997)supported by a grant from the Ministry of Trade,Industry&Energy(MOTIE,Korea)under Industrial Technology Innovation Program(20009652:Technology on commercialization and materials of Bioabsorbable Hydroxyapatite that is less than micrometer in size).
文摘Volumetric muscle loss(VML)is associated with a severe loss of muscle tissue that overwhelms the regenerative potential of skeletal muscles.Tissue engineering has shown promise for the treatment of VML injuries,as evidenced by various preclinical trials.The present study describes the fabrication of a cell-laden GelMa muscle construct using an in situ crosslinking(ISC)strategy to improve muscle functionality.To obtain optimal biophysical properties of the muscle construct,two UV exposure sources,UV exposure dose,and wall shear stress were evaluated using C2C12 myoblasts.Additionally,the ISC system showed a significantly higher degree of uniaxial alignment and myogenesis compared to the conventional crosslinking strategy(post-crosslinking).To evaluate the in vivo regenerative potential,muscle constructs laden with human adipose stem cells were used.The VML defect group implanted with the bio-printed muscle construct showed significant restoration of functionality and muscular volume.The data presented in this study suggest that stem cell-based therapies combined with the modified bioprinting process could potentially be effective against VML injuries.