Precise assembly of biomolecules into functional aggregate structures represents a key characteristic of nature living cells,and is critical for the cellular processes.The construction of artificial aggregates in livin...Precise assembly of biomolecules into functional aggregate structures represents a key characteristic of nature living cells,and is critical for the cellular processes.The construction of artificial aggregates in living cells by responding to intracel-lular specific stimuli has been applied in elucidating molecular mechanisms of naturally cellular processes and interfering with cellular processes,which is poten-tial and significant in biomedicine.DNA(deoxyribonucleic acid)features sequence programmability,precise assembly and versatility,and therefore is regarded as the potential candidate for constructing versatile functional aggregates in living cells.In this review,we summarize our recent efforts of employing DNA to in situ construct versatile aggregates in living cells via responding intracellular triggers,and the sub-sequent bio-interference of the DNA aggregates.Finally,we discuss the remaining challenges and opportunities in thefield,and envision that rational design and con-struction of versatile DNA aggregates in living system would be a promising solution for precision and personalized therapeutics.展开更多
基金National Natural Science Foundation of China,Grant/Award Numbers:22225505,22105050China Postdoctoral Science Foundation,Grant/Award Number:2021M70079Special Research Assistant Project of Chinese Academy of Sciences,Grant/Award Number:E2F2511。
文摘Precise assembly of biomolecules into functional aggregate structures represents a key characteristic of nature living cells,and is critical for the cellular processes.The construction of artificial aggregates in living cells by responding to intracel-lular specific stimuli has been applied in elucidating molecular mechanisms of naturally cellular processes and interfering with cellular processes,which is poten-tial and significant in biomedicine.DNA(deoxyribonucleic acid)features sequence programmability,precise assembly and versatility,and therefore is regarded as the potential candidate for constructing versatile functional aggregates in living cells.In this review,we summarize our recent efforts of employing DNA to in situ construct versatile aggregates in living cells via responding intracellular triggers,and the sub-sequent bio-interference of the DNA aggregates.Finally,we discuss the remaining challenges and opportunities in thefield,and envision that rational design and con-struction of versatile DNA aggregates in living system would be a promising solution for precision and personalized therapeutics.
