To explore the application effect of bio-organic fertilizer Kunyijian in flue-cured tobacco and provide scientific basis for its application in production,a plot trial was conducted with K326,a flue-cured tobacco vari...To explore the application effect of bio-organic fertilizer Kunyijian in flue-cured tobacco and provide scientific basis for its application in production,a plot trial was conducted with K326,a flue-cured tobacco variety,in the Science and Technology Test Base of Xundian County,Yunnan.Taking local fertilizer consumption(control 1)and 70%local fertilizer consumption(control 2)as controls,the following replacement groups were designed:under uniformly replacement 70%local fertilizer consumption,(i)organic fertilizer∶microbial agent=80∶1(kg),+600 kg/ha,+900 kg/ha,+1200 kg/ha,+1500 kg/ha Kunyijian;(ii)organic fertilizer∶microbial agent=40∶1(kg),+900 kg/ha Kunyijian.The results show that in the treatment of 70%local fertilizer consumption+1200 kg/ha Kunyijian[organic fertilizer∶microbial agent=80∶1(kg)],the comprehensive performance of flue-cured tobacco was significantly better than that in the control groups,the yield of tobacco leaves reached 2237.1 kg/ha,the output value was 45505.2 yuan/ha,and the average price of the tobacco leaves was 20.53 yuan/kg.The performance of flue-cured tobacco in the treatment of 70%local fertilizer consumption+1500 kg/ha Kunyijian ranked second.The performance of other fertilization treatments was not good,but it did not differ significantly from that of control 1.In terms of chemical composition of tobacco leaves,partial replacement of chemical fertilizer by bio-organic fertilizer Kunyijian,was beneficial to increase the sugar content and reduce the nicotine and total nitrogen contents in tobacco leaves,especially the treatment of 70%local fertilizer consumption+900 kg/ha Kunyijian[organic fertilizer∶microbial agent=80∶1(kg)],and it was also beneficial to increase the chlorine content in tobacco leaves,especially the treatment of 70%local fertilizer consumption+1500 kg/ha Kunyijian[organic fertilizer∶microbial agent=80∶1(kg)].Therefore,it is feasible to use the bio-organic fertilizer Kunyijian to replace 30%of chemical fertilizer in tobacco production.The rate is recommended to be 900-1500 kg/ha[organic fertilizer∶microbial agent=80∶1(kg)].It is suggested to further strengthen the demonstration and promotion of Kunyijian.展开更多
A field experiment was conducted to evaluate the effects of self-developed full-element bio-organic fertilizer on the growth,yield,and rhizosphere soil nutrients of pepper.Four treatments were designed,including full-...A field experiment was conducted to evaluate the effects of self-developed full-element bio-organic fertilizer on the growth,yield,and rhizosphere soil nutrients of pepper.Four treatments were designed,including full-element bio-organic fertilizer+conventional fertilizer reduced by 50%(T1),inactivated full-element bio-organic fertilizer+conventional fertilizer reduced by 50%(T2),conventional fertilizer(T3),and no fertilizer(CK).The results showed that T1 significantly increased the plant height,crown width,fruit number per plant,and yield of pepper.T1 had higher pH value,total nitrogen,total phosphorus,total potassium,available nitrogen,available phosphorus,and available potassium in the rhizosphere soil than T3 and CK,and it had higher available phosphorus and available potassium than T2.The disease index of bacterial wilt in T1 was 21.74,which was 10.37,20.19,and 35.48 lower than T2,T3,and CK,respectively.The control effect of T1 reached 56.71%.The above results indicated that whole bio-organic fertilizer promoted the growth to improve the yield and benefit of pepper.Moreover,the fertilizer activated soil nutrients to improve soil fertility and reduced soil-borne diseases.Therefore,the full-element bio-organic fertilizer can be promoted in the pepper fields with continuous cropping obstacles.展开更多
[Objective] This study aimed to investigate the effects of seaweed bio-or- ganic fertilizer on yield and quality of winter wheat. [Method] Seaweed bio-organic fertilizer was applied to leaves of winter wheat according...[Objective] This study aimed to investigate the effects of seaweed bio-or- ganic fertilizer on yield and quality of winter wheat. [Method] Seaweed bio-organic fertilizer was applied to leaves of winter wheat according to the dose of 45 kg/hm^2 from jointing stage to maturing stage, and plant height, dry matter accumulation, flag leaf photosynthetic characteristics and grain yield of winter wheat were investigated. [Result] Foliar spraying of seaweed bio-organic fertilizer showed little effect on plant height of winter wheat, thickened stems, promoted dry matter accumulation, in- creased flag leaf photosynthetic rate by 3.16%, and increased yield of winter wheat by 6.85%. [Conclusion] Foliar spraying of seaweed bio-organic fertilizer promoted the intelligent growth, thickened the stems, improved the lodging resistance, significantly increased the panicle weight per plant, and increased the bulk density of winter wheat, as well as improving the physical quality of wheat grain. In addition, foliar spraying of seaweed bio-organic fertilizer promoted the synthesis of chlorophyll and mitigated the decomposition of chlorophyll in winter wheat. Under the background of fertilizer-pesticide double reduction, the test results and data of this study can be promoted in the wheat-growing areas of Shandong Province and even whole China.展开更多
[Objective] This study aimed to investigate the characteristics of bio-organic fertilizer and its effect when applied to peach.[Method] Through launching demonstration trial on the application of bio-organic fertilize...[Objective] This study aimed to investigate the characteristics of bio-organic fertilizer and its effect when applied to peach.[Method] Through launching demonstration trial on the application of bio-organic fertilizer in the major fruit producing areas in Liaoning Province,the effects of bio-organic fertilizer on peach growth and soil were investigated.[Results] After application of bio-organic fertilizer,both the peach yield and fruit quality were improved to some extent,of which yields was increased by 16.4% compared with the control,and vitamin C and total sugar contents were also significantly increased; application of bio-organic fertilizer also improved the contents of total nitrogen,rapidly available phosphorus,available potassium and organic matter in soil,and reduced the soil volume weight.[Conclusion] Bioorganic fertilizer can significantly improve fruit yield and quality,as well as improving orchard soil and protecting the environment,thus possessing a bright application prospect in the production of green fruits.展开更多
[Objective] This study aimed to investigate the effects of liquid seaweed bio-organic fertilizer on seed germination and seedling growth of different vegetables. [Method] Serial dilution concentrations of liquid seawe...[Objective] This study aimed to investigate the effects of liquid seaweed bio-organic fertilizer on seed germination and seedling growth of different vegetables. [Method] Serial dilution concentrations of liquid seaweed bio-organic fertilizer were prepared for seed soaking and pot incubation of cucumber, tomato and chili, to ob- serve the effects of liquid seaweed bio-organic fertilizer on seed germination and seedling growth of vegetables. [Result] Compared with the control, germination rate of cucumber and tomato seeds applied with 600-fold liquid seaweed bio-organic fer- tilizer varied significantly; germination rate of chili seeds applied with 400-fold liquid seaweed bio-organic fertilizer varied significantly; germination energy and germination index of chili seeds applied with different dilution concentrations of liquid seaweed bio-organic fertilizer presented no significant differences. In addition, 200-fold and 400-fold liquid seaweed bio-organic fertilizer significantly improved the root length, plant height, plant fresh weight, plant dry weight, chlorophyll content and leaf area of cucumber, tomato and chili seedlings; after treated with 600-fold liquid seaweed bio-organic fertilizer, root length, chlorophyll content and leaf area of cucumber seedlings varied significantly compared with the control, but no significant differences were observed in plant height, plant fresh weight and plant dry weight; after treated with 600-fold liquid seaweed bio-organic fertilizer, root length, chlorophyll content, plant height, plant fresh weight and plant dry weight of chili and tomato varied sig- nificantly compared with the control, but no significant differences were observed in leaf area. [Conclusion] Soaking vegetable seeds with liquid seaweed bio-organic fer- tilizer can significantly improve seed generation rate and seedling growth.展开更多
In this study, the effects of bio-organic fertilizer with antagonistic bacteria against tobacco bacterial wilt on soil microbial communities and disease resist- ance of tobacco were investigated by field experiment. T...In this study, the effects of bio-organic fertilizer with antagonistic bacteria against tobacco bacterial wilt on soil microbial communities and disease resist- ance of tobacco were investigated by field experiment. The results showed that the incidence of tobacco bacterial wilt in bio-organic fertilizer treatments (T3 and T4) decreased remarkably among four treatments in the field. Compared with the local conventional fertilization group, the incidence of tobacco bacterial wilt was re- duced by 21.9% and 25.0% in T3 and T4, respectively ; the yield of flue-cured tobacco was improved by 5.7% and 5.3%, respectively ; the proportion of mid- high grade tobacco leaves increased by 2.3% and 2.6%, respectively. After application of bio-organie fertilizer with antagonistic bacteria against tobacco bacterial wilt, rhizosphere soil microbial communities exhibited vast amount and abundant species ; the amount of rhizosphere soil bacteria of infected tobacco plants was im- proved by 218.5% with fewer species. It could be concluded that the application of bio-organic fertilizer with antagonistic bacteria against tobacco bacterial wilt could improve the ecological environment of tobacco field, inhibit the growth of pathogenic bacteria, decrease the incidence of tobacco bacterial wilt, and enhance the quality of flue-cured tobacco. This study laid the foundation for further ecological prevention and control of soil-borne diseases of tobacco.展开更多
With strawberry as a test material,the effects of the bio-organic fertilizer containing high-efficiency nitrogen-fixing Bacillus amyloliquefaciens on nutrient contents in strawberry-planted soil,wilt occurrence and st...With strawberry as a test material,the effects of the bio-organic fertilizer containing high-efficiency nitrogen-fixing Bacillus amyloliquefaciens on nutrient contents in strawberry-planted soil,wilt occurrence and strawberry yield and quality were studied by a plot experiment,so as to provide reference for scientific use of the bio-organic fertilizer and green production of strawberry.The results showed that after hole-applying the bio-organic fertilizer at a rate of 22.5 t/hm^2,the contents of NH^+_4-N,available P,available K and organic matter did not change much with time;and when replacing 50%of chemical fertilizers with the bio-organic fertilizer at a rate of 11.25 t/hm^2(K_3),the contents of NH^+_4-N and available P in the soil did not change much with time,and the contents of available K and organic matter decreased slightly with time,but were both higher than the CK(the unfertilized treatment).Meanwhile,the disease index values of strawberry wilt disease in treatments K_2 and K_3were significantly lower than those of the CK and the conventional fertilization treatment(K_1),and the vitamin C contents of strawberry fruit in the two treatments were significantly higher than that of the CK.The yield determination showed that the cumulative yields of treatments K_2 and K_3 increased by 9.8% and 3.3%,respectively,and the increase rates of the early yields(before the Spring Festival)were 30.6% and 21.9%,respectively.Therefore,the application of the bio-organic fertilizer can replace chemical fertilizers,and can achieve the effects of reducing the occurrence of wilt,improving the early yield of fruit commodity and improving fruit quality.展开更多
Through the application of bio-organic fertilizer on Chuanzhuo 16, the re- sults showed that the yield could reach 185.23 kg in treatment with bio-organic fer- tilizer, 40.22 kg more than the conventional fertilizatio...Through the application of bio-organic fertilizer on Chuanzhuo 16, the re- sults showed that the yield could reach 185.23 kg in treatment with bio-organic fer- tilizer, 40.22 kg more than the conventional fertilization, and the yield increase rate reached up to 27.7%.展开更多
Continuous cropping obstacles hamper the efficient growth and yield of Szechuan pepper,Zanthoxylum simulans.The current study investigated the impact of different levels of bioorganic fertilizer on the leaf physiologi...Continuous cropping obstacles hamper the efficient growth and yield of Szechuan pepper,Zanthoxylum simulans.The current study investigated the impact of different levels of bioorganic fertilizer on the leaf physiological and photosynthetic characteristics of Z.simulans to provide a theoretical reference for continuous Z.simulans crop cultivation.A bioorganic fertilizer was used to treat seedlings growing in 25-year-old continuous cropping soil.Five fertilizer treatments were applied.The impacts of the treatments on the activity of defense enzyme and photosynthetic parameters of Z.simulans leaves were determined.The different concentrations of bioorganic fertilizer reduced to varying degrees the malondialdehyde(MDA)content and intercellular CO2 concentration(Ci),and increased the activity of peroxidase(POD),superoxide dismutase(SOD),and ascorbate peroxidase(APX),as well as the chlorophyll content,net photosynthetic rate(Pn),stomatal conductance(Gs)and transpiration rate(Tr)of Z.simulans leaves.The results showed that most significant increases or decreases were achieved with 100 g/L bioorganic fertilizer(Y2).Thus,the application of bioorganic fertilizer at a rate of 100 g/L can significantly improve the activity of relevant defense enzymes and photosynthetic parameters of Z.simulans,and reduce the MDA content,enhancing the stress resistance of the plants,promoting their growth and addressing,to some extent,obstacles associated with continuous cultivation.展开更多
有机肥部分替代化肥是一种实现化肥减量的可持续农业生产措施。本研究于2020和2021连续2年以“沁黄2号”为供试材料,设不施氮(CK)、常量化肥氮(NPK)、有机肥替代25%化肥氮(25%M)、有机肥替代50%化肥氮(50%M)、有机肥替代75%化肥氮(75%M...有机肥部分替代化肥是一种实现化肥减量的可持续农业生产措施。本研究于2020和2021连续2年以“沁黄2号”为供试材料,设不施氮(CK)、常量化肥氮(NPK)、有机肥替代25%化肥氮(25%M)、有机肥替代50%化肥氮(50%M)、有机肥替代75%化肥氮(75%M)和有机肥替代100%化肥氮(100%M) 6个处理,研究不同替代率对谷子产量构成和氮素吸收的影响,并分析氮素吸收调控小米米色、糊化特性和类胡萝卜素组分的效应,明确谷子生产中有机肥最佳替代率。结果表明,2年中较低的有机肥替代化肥氮率显著提高了谷子植株氮素累积,但随着替代率的持续增加,植株氮素累积量呈降低趋势,最终影响谷子产量和小米品质。2020年, 25%M处理显著提高了谷子地上部氮素累积量,较NPK处理提高9.6%;2021年,25%M处理谷子地上部氮素累积量、生物量、穗粒数和产量达到最高,较NPK处理分别提高6.1%、12.0%、15.4%和12.0%。50%M处理显著影响了小米的米色、糊化特征和类胡萝卜素含量,与NPK相比,小米籽粒红绿值、橘色值、支链淀粉含量、总淀粉含量、小米糊化最终黏度、叶黄素含量、玉米黄质含量和黄色素含量的增幅分别可达6.0%、6.0%、7.4%、4.3%、7.8%、20.7%、17.4%和2.8%。但有机肥完全替代化肥降低了谷子地上部氮素吸收、地上部生物量、穗粒数及谷子产量,也抑制了小米叶黄素和玉米黄质含量的提升。2年均表现出谷子地上部氮吸收量与小米单粒重、直链淀粉含量和小米糊化回升值呈显著的负相关。且2021年谷子地上部氮吸收量还与小米总淀粉含量、蛋白质含量、小米粉糊化峰值黏度和小米黄色素含量呈显著的负相关,与小米糊化峰谷黏度呈显著的正相关。综上,施氮总量120 kg hm^(–2)下,有机肥替代25%~50%化肥氮能通过促进植株氮素的吸收,实现产量、米色、蒸煮特性及类胡萝卜素的协同提升,为谷子化肥减量和提质增效生产提供技术支撑。展开更多
Rational application of nitrogen (N) fertilizers is an important measure to raise N fertilizer recovery rate and reduce N loss.A two-year field experiment of rice-wheat rotation was employed to study the effects of ...Rational application of nitrogen (N) fertilizers is an important measure to raise N fertilizer recovery rate and reduce N loss.A two-year field experiment of rice-wheat rotation was employed to study the effects of N fertilization modes including a N fertilizer reduction and an organic manure replacement on crop yield,nutrient uptake,soil enzyme activity,and number of microbes as well as diversity of microbes.The result showed that 20% reduction of traditional N fertilizer dose of local farmers did not significantly change crop yield,N uptake,soil enzyme activity,and the number of microbes (bacteria,actinomycetes,and fungi).On the basis of 20% reduction of N fertilizer,50% replacement of N fertilizer by organic manure increased the activity of sucrose,protease,urease,and phosphatase by 46-62,27-89,33-46,and 35-74%,respectively,and the number of microbes,i.e.,bacteria,actinomycetes,and fungi by 36-150,11-153,and 43-56%,respectively.Further,organic fertilizer replacement had a Shannon's diversity index (H) of 2.18,which was higher than that of other modes of single N fertilizer application.The results suggested that reducing N fertilizer by 20% and applying organic manure in the experimental areas could effectively lower the production costs and significantly improve soil fertility and biological properties.展开更多
基金Key Project of Yunnan Branch of China National Tobacco Corporation(Research and Integrated Application of Key Techniques for Quality Promotion of Original Honghua Dajinyuan).
文摘To explore the application effect of bio-organic fertilizer Kunyijian in flue-cured tobacco and provide scientific basis for its application in production,a plot trial was conducted with K326,a flue-cured tobacco variety,in the Science and Technology Test Base of Xundian County,Yunnan.Taking local fertilizer consumption(control 1)and 70%local fertilizer consumption(control 2)as controls,the following replacement groups were designed:under uniformly replacement 70%local fertilizer consumption,(i)organic fertilizer∶microbial agent=80∶1(kg),+600 kg/ha,+900 kg/ha,+1200 kg/ha,+1500 kg/ha Kunyijian;(ii)organic fertilizer∶microbial agent=40∶1(kg),+900 kg/ha Kunyijian.The results show that in the treatment of 70%local fertilizer consumption+1200 kg/ha Kunyijian[organic fertilizer∶microbial agent=80∶1(kg)],the comprehensive performance of flue-cured tobacco was significantly better than that in the control groups,the yield of tobacco leaves reached 2237.1 kg/ha,the output value was 45505.2 yuan/ha,and the average price of the tobacco leaves was 20.53 yuan/kg.The performance of flue-cured tobacco in the treatment of 70%local fertilizer consumption+1500 kg/ha Kunyijian ranked second.The performance of other fertilization treatments was not good,but it did not differ significantly from that of control 1.In terms of chemical composition of tobacco leaves,partial replacement of chemical fertilizer by bio-organic fertilizer Kunyijian,was beneficial to increase the sugar content and reduce the nicotine and total nitrogen contents in tobacco leaves,especially the treatment of 70%local fertilizer consumption+900 kg/ha Kunyijian[organic fertilizer∶microbial agent=80∶1(kg)],and it was also beneficial to increase the chlorine content in tobacco leaves,especially the treatment of 70%local fertilizer consumption+1500 kg/ha Kunyijian[organic fertilizer∶microbial agent=80∶1(kg)].Therefore,it is feasible to use the bio-organic fertilizer Kunyijian to replace 30%of chemical fertilizer in tobacco production.The rate is recommended to be 900-1500 kg/ha[organic fertilizer∶microbial agent=80∶1(kg)].It is suggested to further strengthen the demonstration and promotion of Kunyijian.
基金Supported by National Nature Science Foundation of China(32000047)Selecting the Best Candidates for Making Technological Breakthroughs in Hunan Province(2021NK1040)Natural Science Foundation of Changsha City(kq2208130)。
文摘A field experiment was conducted to evaluate the effects of self-developed full-element bio-organic fertilizer on the growth,yield,and rhizosphere soil nutrients of pepper.Four treatments were designed,including full-element bio-organic fertilizer+conventional fertilizer reduced by 50%(T1),inactivated full-element bio-organic fertilizer+conventional fertilizer reduced by 50%(T2),conventional fertilizer(T3),and no fertilizer(CK).The results showed that T1 significantly increased the plant height,crown width,fruit number per plant,and yield of pepper.T1 had higher pH value,total nitrogen,total phosphorus,total potassium,available nitrogen,available phosphorus,and available potassium in the rhizosphere soil than T3 and CK,and it had higher available phosphorus and available potassium than T2.The disease index of bacterial wilt in T1 was 21.74,which was 10.37,20.19,and 35.48 lower than T2,T3,and CK,respectively.The control effect of T1 reached 56.71%.The above results indicated that whole bio-organic fertilizer promoted the growth to improve the yield and benefit of pepper.Moreover,the fertilizer activated soil nutrients to improve soil fertility and reduced soil-borne diseases.Therefore,the full-element bio-organic fertilizer can be promoted in the pepper fields with continuous cropping obstacles.
基金Supported by Key Project of the National Twelfth Five-Year Research Program of China(2011BAD32B02)Crosswise Project of Shandong Shidai Marine Biological Technology(Weihai)Co.,Ltd.(2015-2017)~~
文摘[Objective] This study aimed to investigate the effects of seaweed bio-or- ganic fertilizer on yield and quality of winter wheat. [Method] Seaweed bio-organic fertilizer was applied to leaves of winter wheat according to the dose of 45 kg/hm^2 from jointing stage to maturing stage, and plant height, dry matter accumulation, flag leaf photosynthetic characteristics and grain yield of winter wheat were investigated. [Result] Foliar spraying of seaweed bio-organic fertilizer showed little effect on plant height of winter wheat, thickened stems, promoted dry matter accumulation, in- creased flag leaf photosynthetic rate by 3.16%, and increased yield of winter wheat by 6.85%. [Conclusion] Foliar spraying of seaweed bio-organic fertilizer promoted the intelligent growth, thickened the stems, improved the lodging resistance, significantly increased the panicle weight per plant, and increased the bulk density of winter wheat, as well as improving the physical quality of wheat grain. In addition, foliar spraying of seaweed bio-organic fertilizer promoted the synthesis of chlorophyll and mitigated the decomposition of chlorophyll in winter wheat. Under the background of fertilizer-pesticide double reduction, the test results and data of this study can be promoted in the wheat-growing areas of Shandong Province and even whole China.
文摘[Objective] This study aimed to investigate the characteristics of bio-organic fertilizer and its effect when applied to peach.[Method] Through launching demonstration trial on the application of bio-organic fertilizer in the major fruit producing areas in Liaoning Province,the effects of bio-organic fertilizer on peach growth and soil were investigated.[Results] After application of bio-organic fertilizer,both the peach yield and fruit quality were improved to some extent,of which yields was increased by 16.4% compared with the control,and vitamin C and total sugar contents were also significantly increased; application of bio-organic fertilizer also improved the contents of total nitrogen,rapidly available phosphorus,available potassium and organic matter in soil,and reduced the soil volume weight.[Conclusion] Bioorganic fertilizer can significantly improve fruit yield and quality,as well as improving orchard soil and protecting the environment,thus possessing a bright application prospect in the production of green fruits.
文摘[Objective] This study aimed to investigate the effects of liquid seaweed bio-organic fertilizer on seed germination and seedling growth of different vegetables. [Method] Serial dilution concentrations of liquid seaweed bio-organic fertilizer were prepared for seed soaking and pot incubation of cucumber, tomato and chili, to ob- serve the effects of liquid seaweed bio-organic fertilizer on seed germination and seedling growth of vegetables. [Result] Compared with the control, germination rate of cucumber and tomato seeds applied with 600-fold liquid seaweed bio-organic fer- tilizer varied significantly; germination rate of chili seeds applied with 400-fold liquid seaweed bio-organic fertilizer varied significantly; germination energy and germination index of chili seeds applied with different dilution concentrations of liquid seaweed bio-organic fertilizer presented no significant differences. In addition, 200-fold and 400-fold liquid seaweed bio-organic fertilizer significantly improved the root length, plant height, plant fresh weight, plant dry weight, chlorophyll content and leaf area of cucumber, tomato and chili seedlings; after treated with 600-fold liquid seaweed bio-organic fertilizer, root length, chlorophyll content and leaf area of cucumber seedlings varied significantly compared with the control, but no significant differences were observed in plant height, plant fresh weight and plant dry weight; after treated with 600-fold liquid seaweed bio-organic fertilizer, root length, chlorophyll content, plant height, plant fresh weight and plant dry weight of chili and tomato varied sig- nificantly compared with the control, but no significant differences were observed in leaf area. [Conclusion] Soaking vegetable seeds with liquid seaweed bio-organic fer- tilizer can significantly improve seed generation rate and seedling growth.
基金Supported by Project of Nanping Tobacco Monopoly Bureau(NYK2012-14-3)
文摘In this study, the effects of bio-organic fertilizer with antagonistic bacteria against tobacco bacterial wilt on soil microbial communities and disease resist- ance of tobacco were investigated by field experiment. The results showed that the incidence of tobacco bacterial wilt in bio-organic fertilizer treatments (T3 and T4) decreased remarkably among four treatments in the field. Compared with the local conventional fertilization group, the incidence of tobacco bacterial wilt was re- duced by 21.9% and 25.0% in T3 and T4, respectively ; the yield of flue-cured tobacco was improved by 5.7% and 5.3%, respectively ; the proportion of mid- high grade tobacco leaves increased by 2.3% and 2.6%, respectively. After application of bio-organie fertilizer with antagonistic bacteria against tobacco bacterial wilt, rhizosphere soil microbial communities exhibited vast amount and abundant species ; the amount of rhizosphere soil bacteria of infected tobacco plants was im- proved by 218.5% with fewer species. It could be concluded that the application of bio-organic fertilizer with antagonistic bacteria against tobacco bacterial wilt could improve the ecological environment of tobacco field, inhibit the growth of pathogenic bacteria, decrease the incidence of tobacco bacterial wilt, and enhance the quality of flue-cured tobacco. This study laid the foundation for further ecological prevention and control of soil-borne diseases of tobacco.
文摘With strawberry as a test material,the effects of the bio-organic fertilizer containing high-efficiency nitrogen-fixing Bacillus amyloliquefaciens on nutrient contents in strawberry-planted soil,wilt occurrence and strawberry yield and quality were studied by a plot experiment,so as to provide reference for scientific use of the bio-organic fertilizer and green production of strawberry.The results showed that after hole-applying the bio-organic fertilizer at a rate of 22.5 t/hm^2,the contents of NH^+_4-N,available P,available K and organic matter did not change much with time;and when replacing 50%of chemical fertilizers with the bio-organic fertilizer at a rate of 11.25 t/hm^2(K_3),the contents of NH^+_4-N and available P in the soil did not change much with time,and the contents of available K and organic matter decreased slightly with time,but were both higher than the CK(the unfertilized treatment).Meanwhile,the disease index values of strawberry wilt disease in treatments K_2 and K_3were significantly lower than those of the CK and the conventional fertilization treatment(K_1),and the vitamin C contents of strawberry fruit in the two treatments were significantly higher than that of the CK.The yield determination showed that the cumulative yields of treatments K_2 and K_3 increased by 9.8% and 3.3%,respectively,and the increase rates of the early yields(before the Spring Festival)were 30.6% and 21.9%,respectively.Therefore,the application of the bio-organic fertilizer can replace chemical fertilizers,and can achieve the effects of reducing the occurrence of wilt,improving the early yield of fruit commodity and improving fruit quality.
文摘Through the application of bio-organic fertilizer on Chuanzhuo 16, the re- sults showed that the yield could reach 185.23 kg in treatment with bio-organic fer- tilizer, 40.22 kg more than the conventional fertilization, and the yield increase rate reached up to 27.7%.
基金This study was supported by the Central Committee for guiding the local science and technology development sub-project“Study on the Structural Adjustment and Optimization Research and Platform Construction of the Characteristic Economic Forest and Pepper in Longnan”,and thanks for the International Science Editing(http://www.Internationalscienceediting.com)for editing this manuscript.
文摘Continuous cropping obstacles hamper the efficient growth and yield of Szechuan pepper,Zanthoxylum simulans.The current study investigated the impact of different levels of bioorganic fertilizer on the leaf physiological and photosynthetic characteristics of Z.simulans to provide a theoretical reference for continuous Z.simulans crop cultivation.A bioorganic fertilizer was used to treat seedlings growing in 25-year-old continuous cropping soil.Five fertilizer treatments were applied.The impacts of the treatments on the activity of defense enzyme and photosynthetic parameters of Z.simulans leaves were determined.The different concentrations of bioorganic fertilizer reduced to varying degrees the malondialdehyde(MDA)content and intercellular CO2 concentration(Ci),and increased the activity of peroxidase(POD),superoxide dismutase(SOD),and ascorbate peroxidase(APX),as well as the chlorophyll content,net photosynthetic rate(Pn),stomatal conductance(Gs)and transpiration rate(Tr)of Z.simulans leaves.The results showed that most significant increases or decreases were achieved with 100 g/L bioorganic fertilizer(Y2).Thus,the application of bioorganic fertilizer at a rate of 100 g/L can significantly improve the activity of relevant defense enzymes and photosynthetic parameters of Z.simulans,and reduce the MDA content,enhancing the stress resistance of the plants,promoting their growth and addressing,to some extent,obstacles associated with continuous cultivation.
文摘有机肥部分替代化肥是一种实现化肥减量的可持续农业生产措施。本研究于2020和2021连续2年以“沁黄2号”为供试材料,设不施氮(CK)、常量化肥氮(NPK)、有机肥替代25%化肥氮(25%M)、有机肥替代50%化肥氮(50%M)、有机肥替代75%化肥氮(75%M)和有机肥替代100%化肥氮(100%M) 6个处理,研究不同替代率对谷子产量构成和氮素吸收的影响,并分析氮素吸收调控小米米色、糊化特性和类胡萝卜素组分的效应,明确谷子生产中有机肥最佳替代率。结果表明,2年中较低的有机肥替代化肥氮率显著提高了谷子植株氮素累积,但随着替代率的持续增加,植株氮素累积量呈降低趋势,最终影响谷子产量和小米品质。2020年, 25%M处理显著提高了谷子地上部氮素累积量,较NPK处理提高9.6%;2021年,25%M处理谷子地上部氮素累积量、生物量、穗粒数和产量达到最高,较NPK处理分别提高6.1%、12.0%、15.4%和12.0%。50%M处理显著影响了小米的米色、糊化特征和类胡萝卜素含量,与NPK相比,小米籽粒红绿值、橘色值、支链淀粉含量、总淀粉含量、小米糊化最终黏度、叶黄素含量、玉米黄质含量和黄色素含量的增幅分别可达6.0%、6.0%、7.4%、4.3%、7.8%、20.7%、17.4%和2.8%。但有机肥完全替代化肥降低了谷子地上部氮素吸收、地上部生物量、穗粒数及谷子产量,也抑制了小米叶黄素和玉米黄质含量的提升。2年均表现出谷子地上部氮吸收量与小米单粒重、直链淀粉含量和小米糊化回升值呈显著的负相关。且2021年谷子地上部氮吸收量还与小米总淀粉含量、蛋白质含量、小米粉糊化峰值黏度和小米黄色素含量呈显著的负相关,与小米糊化峰谷黏度呈显著的正相关。综上,施氮总量120 kg hm^(–2)下,有机肥替代25%~50%化肥氮能通过促进植株氮素的吸收,实现产量、米色、蒸煮特性及类胡萝卜素的协同提升,为谷子化肥减量和提质增效生产提供技术支撑。
基金supported by the National Basic Research Program of China (973 Program,2007CB109308)the National High-Tech R&D Program of China (2007AA06Z332)a special grant of Application of Nuclear Techniques in Agriculture from the Ministry of Agriculture of China (200803034)
文摘Rational application of nitrogen (N) fertilizers is an important measure to raise N fertilizer recovery rate and reduce N loss.A two-year field experiment of rice-wheat rotation was employed to study the effects of N fertilization modes including a N fertilizer reduction and an organic manure replacement on crop yield,nutrient uptake,soil enzyme activity,and number of microbes as well as diversity of microbes.The result showed that 20% reduction of traditional N fertilizer dose of local farmers did not significantly change crop yield,N uptake,soil enzyme activity,and the number of microbes (bacteria,actinomycetes,and fungi).On the basis of 20% reduction of N fertilizer,50% replacement of N fertilizer by organic manure increased the activity of sucrose,protease,urease,and phosphatase by 46-62,27-89,33-46,and 35-74%,respectively,and the number of microbes,i.e.,bacteria,actinomycetes,and fungi by 36-150,11-153,and 43-56%,respectively.Further,organic fertilizer replacement had a Shannon's diversity index (H) of 2.18,which was higher than that of other modes of single N fertilizer application.The results suggested that reducing N fertilizer by 20% and applying organic manure in the experimental areas could effectively lower the production costs and significantly improve soil fertility and biological properties.