Microcantilever sensors for biochemical detection

Microcantilever is one of the most popular miniaturized structures in micro-electromechanical systems(MEMS).Sensors based on microcantilever are ideal for biochemical detection,since they have high sensitivity,high throughput,good specification,fast response,thus have attracted extensive attentions.A number of devices that are based on static deflections or shifts of resonant frequency of the cantilevers responding to analyte attachment have been demonstrated.This review comprehensively presents state of art of microcantilever sensors working in gaseous and aqueous environments and highlights the challenges and opportunities of microcantilever biochemical sensors.

Terahertz polarization sensing,chirality enhancement,and specific binding based on metasurface sensors for biochemical detection:a review[Invited]

Specific and highly-sensitive biochemical detection technology is particularly important in global epidemics and has critical applications in life science,medical diagnosis,and pharmaceutics.As a newly developed technology,the THz metamaterialbased sensing method is a promising technique for extremely sensitive biomolecular detection.However,due to the significant resonant peaks generated by THz metamaterials,the characteristic absorption peaks of the analyte are usually masked,making it difficult to distinguish enantiomers and specifically identify target biomolecules.Recently,new ways to overcome this limitation have become possible thanks to the emergence of chiral metasurfaces and the polarization sensing method.Additionally,functionalized metasurfaces modified by antibodies or other nanomaterials are also expected to achieve specific sensing with high sensitivity.In this review,we summarize the main advances in THz metamaterials-based sensing from a historical perspective as well as application in chiral recognition and specific detection.Specifically,we introduce the basic theory and key technology of THz polarization spectrum and chiral sensing for biochemical detection,and immune sensing based on biomolecular interaction is also discussed.We mainly focus on chiral recognition and specific sensing using THz metasurface sensors to cover the most recent advances in the topic,which is expected to break through the limitations of traditional THz absorption spectroscopy and chiral spectroscopy in the visible-infrared band and develop into an irreplaceable method for the characterization of biochemical substances.

A Proposed Solution to the Interference of IgG Kappa-Type M Protein on LDL-C Detection

Objective:To explore the interference of monoclonal immunoglobulin(M protein)on the detection of serum LDL-C in patients with multiple myeloma,improve the understanding of this matter,determine and establish the correct method,and provide more accurate clinical results through this case.Methods:A case was selected for analysis by the direct method.Results:The interference of IgG kappa-type M protein on LDL-C detection could not be completely eliminated by the enzymatic method.Conclusion:IgG-type type M protein affects the detection of LDL-C by the enzymatic method;thus,light reagents can be used with the direct method for detection.

In Vivo Degradation Behavior of Porous Composite Scaffolds of Poly(lactide-co-glycolide) and Nano-hydroxyapatite Surface Grafted with Poly(L-lactide)

The biodegradable porous composite scaffold, composed of poly（lactide-co-glycolide） （PLGA） and hydroxyapatite nanoparticles （n-HAP） surface-grafted with poly（L-lactide） （PLLA） （g-HAP） （g-HAP/PLGA）, was fabricated using the solvent casting/particulate leaching method, and its in vivo degradation behavior was investigated by the intramuscular implantation in rabbits. The composite of un-grafted n-HAP/PLGA and neat PLGA were used as controls. The scaffolds had interconnected pore structures with average pore sizes between 137 μm and 148 μm and porosities between 83% and 86%. There was no significant difference in the pore size and porosity among the three scaffolds. Compared with n-HAP/PLGA, the thermo-degradation temperature （Tc） of g-HAP/PLGA decreased while its glass transition temperature （Tg） increased. The weight change, grey value analysis of radiographs and SEM observation showed that the composite scaffolds of g-HAP/PLGA and n-HAP/PLGA showed slower degradation and higher mineralization than the pure PLGA scaffold after the intramuscular implantation. The rapid degradation of PLGA, g-HAP/PLGA and n-HAP/PLGA occurred at 8-12 weeks, 12-16 weeks and 16-20 weeks, respectively. Compared with n-HAP/PLGA, g-HAP/PLGA showed an improved absorption and biomineralization property mostly because of its improved distribution of HAP nanoparticles. The levels of both calcium and phosphorous in serum and urine could be affected to some extent at 3-4 weeks after the implantation of g-HAP/PLGA, but the biochemical detection of serum AST, ALT, ALP, and GGT as well as BUN and CRE showed no obvious influence on the functions of liver and kidney.