A series of batch-type experiments with acetate acid as the primary substrate were performed using enrichment cultures developed from the anaerobic sludge to investigate the effect of acetate acid on tetrachloroethyle...A series of batch-type experiments with acetate acid as the primary substrate were performed using enrichment cultures developed from the anaerobic sludge to investigate the effect of acetate acid on tetrachloroethylene （PCE） biodegradation. Experimental results indicated that acetate acid was an efficient electron donor in affecting the biotransformability of PCE. Trichloroethylene （TCE） was the primary dehalogenation product, and small amounts of dichloroethylenes （DCEs） were also detected. No significant further DCEs degradation was detected. PCE degradation rate in the experiment was 36.6 times faster than background rate in natural groundwater.展开更多
Objective To investigate the biodegradation of tetrachloroethylene （PCE） by acclimated anaerobic sludge using different co-substrates, i.e., glucose, acetate, and lactate as electron donors. Methods HP-6890 gas chro...Objective To investigate the biodegradation of tetrachloroethylene （PCE） by acclimated anaerobic sludge using different co-substrates, i.e., glucose, acetate, and lactate as electron donors. Methods HP-6890 gas chromatograph （GC） in combination with auto-sampler was used to analyze the concentration of PCE and its intermediates, Results PCE could be degraded by reductive dechlorlnation and the degradation reaction conformed to the first-order kinetic equation. The rate constants are klaetate〉kglucose〉kacetate. The PCE degradation rate was the highest in the presence of lactate as an electron donor. Conclusion Lactate is the most suitable electron donor for PCE degradation and the electron donors supplied by co-metabolic substrates are not the limiting factors for PCE degradation,展开更多
Tetrachloroethene （PCE） is biodegraded by reductive dechlorination with co-metabolism substrates under anaerobic conditions. By inoculating sludge from an anaerobic pool, a biodegradation test of PCE is conducted in...Tetrachloroethene （PCE） is biodegraded by reductive dechlorination with co-metabolism substrates under anaerobic conditions. By inoculating sludge from an anaerobic pool, a biodegradation test of PCE is conducted in the anaerobic condition. In the test, several substrates including methanol, ethanol, formate, acetate, lactate and glucose, are conducive to the conversion from PCE to TCE and 1,1-DCE. The results show the microbe can be cultivated well under the anaerobic circumstances of mixture of sewage （sludge） and soil with the index of COD after eleven days. Degradation of PCE accords with one order reaction kinetics equation. The sequence of the reaction rate constant is Kacetate 〉Kglucose 〉 Klactate 〉 Kethanol 〉 Kformate 〉 Kmethanol, and acetate is an outstanding co-metabolism substratum whose reaction rate constant is 0.6632d^-1.展开更多
文摘A series of batch-type experiments with acetate acid as the primary substrate were performed using enrichment cultures developed from the anaerobic sludge to investigate the effect of acetate acid on tetrachloroethylene （PCE） biodegradation. Experimental results indicated that acetate acid was an efficient electron donor in affecting the biotransformability of PCE. Trichloroethylene （TCE） was the primary dehalogenation product, and small amounts of dichloroethylenes （DCEs） were also detected. No significant further DCEs degradation was detected. PCE degradation rate in the experiment was 36.6 times faster than background rate in natural groundwater.
基金This work was supported by the National Science Foundation of China (Grant No. 40102027 50325824 50578151 and BeijingNatural Science Foundation 8052017).
文摘Objective To investigate the biodegradation of tetrachloroethylene （PCE） by acclimated anaerobic sludge using different co-substrates, i.e., glucose, acetate, and lactate as electron donors. Methods HP-6890 gas chromatograph （GC） in combination with auto-sampler was used to analyze the concentration of PCE and its intermediates, Results PCE could be degraded by reductive dechlorlnation and the degradation reaction conformed to the first-order kinetic equation. The rate constants are klaetate〉kglucose〉kacetate. The PCE degradation rate was the highest in the presence of lactate as an electron donor. Conclusion Lactate is the most suitable electron donor for PCE degradation and the electron donors supplied by co-metabolic substrates are not the limiting factors for PCE degradation,
文摘Tetrachloroethene （PCE） is biodegraded by reductive dechlorination with co-metabolism substrates under anaerobic conditions. By inoculating sludge from an anaerobic pool, a biodegradation test of PCE is conducted in the anaerobic condition. In the test, several substrates including methanol, ethanol, formate, acetate, lactate and glucose, are conducive to the conversion from PCE to TCE and 1,1-DCE. The results show the microbe can be cultivated well under the anaerobic circumstances of mixture of sewage （sludge） and soil with the index of COD after eleven days. Degradation of PCE accords with one order reaction kinetics equation. The sequence of the reaction rate constant is Kacetate 〉Kglucose 〉 Klactate 〉 Kethanol 〉 Kformate 〉 Kmethanol, and acetate is an outstanding co-metabolism substratum whose reaction rate constant is 0.6632d^-1.
