Moulds have been reported to destroy volumes of timbers in buildings annually. As a result, timber components within the built environment decline and fail to fulfill their basic requirements. This research focused on...Moulds have been reported to destroy volumes of timbers in buildings annually. As a result, timber components within the built environment decline and fail to fulfill their basic requirements. This research focused on the isolation and evaluation of the prevalence and effects of deteriorating moulds in the rain forest and swampy rain forest regions of Nigeria where the water activity is as high as 0.7. To accomplish this, decayed timber samples were aseptically collected on buildings from six strategic locations. The samples were serially diluted and inoculated onto Sabouraud Dextrose Agar medium in Petal dishes. The Petri dishes were incubated for 72 h at 30 ~C. Thereafter, moulds were isolated through visual and microscopic observations. The commonly encountered moulds were evaluated and analyzed. It was observed that, prevalence of moulds on buildings used for non residential purpose were higher. There was no significant difference between the prevalence on the components located inside the building and those outside the building. Ceiba pentandra exhibited highest degradation while Masonia altissima resisted most. The most deteriorating moulds were Aspergillus, Mucor, Rhizopus and Gliocladium. The deteriorations of Ceiba pentandra, Afzelia africana, Lophira alata, Anogessus leocarpus and Gossweilerodendron balsamiferum timbers under Aspergillus attack were projected.展开更多
Over the last decade,the oriented strand board(OSB)market presented meaningful growth.However,as a woodbased product,because of its anatomical structure and chemical composition,OSB can be damaged by biodeterioration ...Over the last decade,the oriented strand board(OSB)market presented meaningful growth.However,as a woodbased product,because of its anatomical structure and chemical composition,OSB can be damaged by biodeterioration agents.Given that,the biodeterioration of OSB panels must be investigated to improve its durability.In this way,this work analyses the biological resistance against termites(Cryptotermes brevis and Nasutitermes corniger)of heat-treated OSB panels made with Eucalyptus wood glued with vegetable-based polyurethane-an ecofriendly and sustainable adhesive derived from castor oil.Various panels were produced with different layers compositions(face:core:face of 25:50:25 and 30:40:30)in wood mass proportion and were submitted to postproduction heat treatment(at 175℃ and 200℃)replacing the use of chemical insecticides.The influence of the layers variation and heat treatment temperature were evaluated,and these results were compared with commercial panels(made from pinus wood with insecticide).The results showed that the heat treatment did not improve the resistance against termite attack.However,all the experimental panels presented a satisfactory performance that was compatible with the commercial panels produced with insecticide available in the Brazilian market.The combination of Eucalyptus wood and castor oil adhesive to produce OSB,in any variation of layer composition,demonstrated natural resistance against termite attack compatible with the commercial panels,even without using chemical additives to increase durability.展开更多
The start and the course of bio-corrosion are conditioned by many factors which include biological effects like the influence of vegetation and microorganisms causing the deterioration of materials. The influence of b...The start and the course of bio-corrosion are conditioned by many factors which include biological effects like the influence of vegetation and microorganisms causing the deterioration of materials. The influence of bacteria causing the deterioration of concrete has been linked to the generation of biogenic sulphuric and nitric acids which originate in corrosion process by dissolution of calcium containing minerals from the concrete matrices. This paper primarily focuses on the investigation of influence of sulphur-oxidising bacteria Acidithiobacillus thiooxidans and sulphate-reducing bacteria Desulfovibrio desulfuricans at the resistance degree of cement composites. Various concrete composites with 5% addition of black coal fly ash as cement replacement as well as the reference samples without coal fly ash addition were studied in the experiments environments of sewage system proceeded during 90 days. The The laboratory experiments as well as experiments in situ in real corrosion was manifested by surface changes and weight changes of cement composites samples as well as changes in pH values of leachates. Considerable surface changes were detected in all investigated samples by microscopic methods. Crystals precipitated on concrete samples surface were identified by EDX as mixture of gypsum and ettringite. The roughness increases of surface of cement microscopy. composites were determined by confocal laser scanning展开更多
The science and technology interact with the art in several ways. Biotechnological coupled with analytical approaches can play an important role in protecting and preserving cultural heritage for future generations. M...The science and technology interact with the art in several ways. Biotechnological coupled with analytical approaches can play an important role in protecting and preserving cultural heritage for future generations. Many microorganisms influenced by environmental conditions are the main responsible for biological contamination in built heritage. Biocides based on chemical compounds have been used to mitigate this problem. Thus, it is vitally important to develop proper remediation actions based on environmentally innocuous alternative. Bacillus specie is emerging as an optimistic alternative for built heritage treatment due to their capacity to produce secondary metabolites with antagonistic activities against many fungal pathogens. Therefore, the intent of this work was to access a rapid evaluation of antifungal potential of bioactive metabolites produced by Bacillus strains and simultaneously their characterization using spectroscopic (NMR) and chromatographic techniques (LC-ESI-MS). The high antifungal activity obtained for Bacillus sp. active compounds produced in this study confirms the great potential to suppress biodeteriogenic fungi growth on historical artworks. Additionally, the proposed methodology allowed to access bioactive metabolites produced without need of the laborious total previous isolation and could be used as a viable alternative to be employed for screening and production of new green biocides.展开更多
文摘Moulds have been reported to destroy volumes of timbers in buildings annually. As a result, timber components within the built environment decline and fail to fulfill their basic requirements. This research focused on the isolation and evaluation of the prevalence and effects of deteriorating moulds in the rain forest and swampy rain forest regions of Nigeria where the water activity is as high as 0.7. To accomplish this, decayed timber samples were aseptically collected on buildings from six strategic locations. The samples were serially diluted and inoculated onto Sabouraud Dextrose Agar medium in Petal dishes. The Petri dishes were incubated for 72 h at 30 ~C. Thereafter, moulds were isolated through visual and microscopic observations. The commonly encountered moulds were evaluated and analyzed. It was observed that, prevalence of moulds on buildings used for non residential purpose were higher. There was no significant difference between the prevalence on the components located inside the building and those outside the building. Ceiba pentandra exhibited highest degradation while Masonia altissima resisted most. The most deteriorating moulds were Aspergillus, Mucor, Rhizopus and Gliocladium. The deteriorations of Ceiba pentandra, Afzelia africana, Lophira alata, Anogessus leocarpus and Gossweilerodendron balsamiferum timbers under Aspergillus attack were projected.
基金financed by Coordination for the Improvement of Higher Education Personnel,Brazil(CAPES,https://www.gov.br/capes/pt-br)(accessed on 22 September 2024)Finance Code 001(ESS,FDM)+1 种基金Sao Paulo State Research Support Foundation(FAPESP,https://fapesp.br/)(accessed on 22 September 2024)(CIC,grant number 2015/04660-0)National Council for Scientific and Technological Development(CNPq,https://www.gov.br/cnpq/pt-br)(accessed on 22 September 2024)(grant numbers 308937/2021-0(CIC),306576/2020-1(ECB),and 303099/2022-4(JBP)).
文摘Over the last decade,the oriented strand board(OSB)market presented meaningful growth.However,as a woodbased product,because of its anatomical structure and chemical composition,OSB can be damaged by biodeterioration agents.Given that,the biodeterioration of OSB panels must be investigated to improve its durability.In this way,this work analyses the biological resistance against termites(Cryptotermes brevis and Nasutitermes corniger)of heat-treated OSB panels made with Eucalyptus wood glued with vegetable-based polyurethane-an ecofriendly and sustainable adhesive derived from castor oil.Various panels were produced with different layers compositions(face:core:face of 25:50:25 and 30:40:30)in wood mass proportion and were submitted to postproduction heat treatment(at 175℃ and 200℃)replacing the use of chemical insecticides.The influence of the layers variation and heat treatment temperature were evaluated,and these results were compared with commercial panels(made from pinus wood with insecticide).The results showed that the heat treatment did not improve the resistance against termite attack.However,all the experimental panels presented a satisfactory performance that was compatible with the commercial panels produced with insecticide available in the Brazilian market.The combination of Eucalyptus wood and castor oil adhesive to produce OSB,in any variation of layer composition,demonstrated natural resistance against termite attack compatible with the commercial panels,even without using chemical additives to increase durability.
文摘The start and the course of bio-corrosion are conditioned by many factors which include biological effects like the influence of vegetation and microorganisms causing the deterioration of materials. The influence of bacteria causing the deterioration of concrete has been linked to the generation of biogenic sulphuric and nitric acids which originate in corrosion process by dissolution of calcium containing minerals from the concrete matrices. This paper primarily focuses on the investigation of influence of sulphur-oxidising bacteria Acidithiobacillus thiooxidans and sulphate-reducing bacteria Desulfovibrio desulfuricans at the resistance degree of cement composites. Various concrete composites with 5% addition of black coal fly ash as cement replacement as well as the reference samples without coal fly ash addition were studied in the experiments environments of sewage system proceeded during 90 days. The The laboratory experiments as well as experiments in situ in real corrosion was manifested by surface changes and weight changes of cement composites samples as well as changes in pH values of leachates. Considerable surface changes were detected in all investigated samples by microscopic methods. Crystals precipitated on concrete samples surface were identified by EDX as mixture of gypsum and ettringite. The roughness increases of surface of cement microscopy. composites were determined by confocal laser scanning
文摘The science and technology interact with the art in several ways. Biotechnological coupled with analytical approaches can play an important role in protecting and preserving cultural heritage for future generations. Many microorganisms influenced by environmental conditions are the main responsible for biological contamination in built heritage. Biocides based on chemical compounds have been used to mitigate this problem. Thus, it is vitally important to develop proper remediation actions based on environmentally innocuous alternative. Bacillus specie is emerging as an optimistic alternative for built heritage treatment due to their capacity to produce secondary metabolites with antagonistic activities against many fungal pathogens. Therefore, the intent of this work was to access a rapid evaluation of antifungal potential of bioactive metabolites produced by Bacillus strains and simultaneously their characterization using spectroscopic (NMR) and chromatographic techniques (LC-ESI-MS). The high antifungal activity obtained for Bacillus sp. active compounds produced in this study confirms the great potential to suppress biodeteriogenic fungi growth on historical artworks. Additionally, the proposed methodology allowed to access bioactive metabolites produced without need of the laborious total previous isolation and could be used as a viable alternative to be employed for screening and production of new green biocides.