Under the control framework of algae bloom in eutrophic lakes and reservoirs based on biological manipulation, the temperature variable is introduced into ecological modeling to show that it is a necessary condition f...Under the control framework of algae bloom in eutrophic lakes and reservoirs based on biological manipulation, the temperature variable is introduced into ecological modeling to show that it is a necessary condition for the rapid occurrence of algal blooms, and an aquatic ecological model with temperature effect is proposed to describe dynamic relationship between algae and biological manipulation predator. The mathematical theory work mainly investigates the existence and stability of some equilibrium points and some critical conditions for the occurrence of transcritical bifurcation and Hopf bifurcation. The numerical simulation mainly shows the dynamic evolution process of bifurcation dynamics, which can not only verify the validity and feasibility of these theoretical works but also analyze the influence of some key parameters on dynamic behavior evolution. Furthermore, It is worth emphasizing that temperature plays an important role in the coexistence of algae and biological manipulation predators. Moreover, the coexistence mode of algae and biological manipulation predators is discovered by means of dynamic bifurcation evolution. Finally, it is hoped that these research results can provide some reference for the study of aquatic ecosystems.展开更多
Cells are the basic structural and functional units of organisms.Dynamic analysis and manipulation of specific components in living cells would provide valuable information for the study of related biological processe...Cells are the basic structural and functional units of organisms.Dynamic analysis and manipulation of specific components in living cells would provide valuable information for the study of related biological processes.Advances in fluorescence microscopy have allowed real-time monitoring of biological events at the molecular level.Meanwhile,the development of highperformance fluorescence probes has become a critical issue.Functional nucleic acids(FNAs)are oligonucleotides with special chemical and biological functions,and aptamers with excellent molecular recognition capability are one of the most important representatives.They have attracted extensive attention in the field of live-cell study,owing to intrinsic advantages of simple synthesis,convenient modification,low immunogenicity and high programmability.This review focuses on recent research progress in fluorescence imaging and manipulation of cells using FNAs,particularly aptamers,as the molecular tools.Finally,a summary is provided and the related challenges are discussed.展开更多
文摘Under the control framework of algae bloom in eutrophic lakes and reservoirs based on biological manipulation, the temperature variable is introduced into ecological modeling to show that it is a necessary condition for the rapid occurrence of algal blooms, and an aquatic ecological model with temperature effect is proposed to describe dynamic relationship between algae and biological manipulation predator. The mathematical theory work mainly investigates the existence and stability of some equilibrium points and some critical conditions for the occurrence of transcritical bifurcation and Hopf bifurcation. The numerical simulation mainly shows the dynamic evolution process of bifurcation dynamics, which can not only verify the validity and feasibility of these theoretical works but also analyze the influence of some key parameters on dynamic behavior evolution. Furthermore, It is worth emphasizing that temperature plays an important role in the coexistence of algae and biological manipulation predators. Moreover, the coexistence mode of algae and biological manipulation predators is discovered by means of dynamic bifurcation evolution. Finally, it is hoped that these research results can provide some reference for the study of aquatic ecosystems.
基金supported by the National Key Research Program(2018YFC1602900,2019YFA0905800)the National Natural Science Foundation of China(NSFC 21922404)the Science and Technology Project of Hunan Province(2017XK2103,2018JJ3034,2019SK2201,2018RS3035,2020SK3008)。
文摘Cells are the basic structural and functional units of organisms.Dynamic analysis and manipulation of specific components in living cells would provide valuable information for the study of related biological processes.Advances in fluorescence microscopy have allowed real-time monitoring of biological events at the molecular level.Meanwhile,the development of highperformance fluorescence probes has become a critical issue.Functional nucleic acids(FNAs)are oligonucleotides with special chemical and biological functions,and aptamers with excellent molecular recognition capability are one of the most important representatives.They have attracted extensive attention in the field of live-cell study,owing to intrinsic advantages of simple synthesis,convenient modification,low immunogenicity and high programmability.This review focuses on recent research progress in fluorescence imaging and manipulation of cells using FNAs,particularly aptamers,as the molecular tools.Finally,a summary is provided and the related challenges are discussed.
