Reliable and non-invasive diagnostic tools are highly valuable for successful therapeutic strategies for the treatment of Alzheimer's disease(AD). The existence of neurofibrillary tangles(NFTs) consisting of tau p...Reliable and non-invasive diagnostic tools are highly valuable for successful therapeutic strategies for the treatment of Alzheimer's disease(AD). The existence of neurofibrillary tangles(NFTs) consisting of tau protein are one kind of the pathological features of AD, and its level of severity is correlated with the stage of AD.However, no clinically approved positron emission tomography(PET) probe is currently available for selective imaging of neurofibrillary tangles on patients. In this paper, we report our studies on biological characteristics of [18F]-THK523 as a novel tau imaging probe. With low molecular weight, [18F]-THK523 is stable, electrically neutral, lipophilic and non-mass concentration-dependent. Preliminary biological studies have shown the excellent properties of [18F]-THK523 as brain imaging tracer for further research.展开更多
Fluorescence imaging is a non-invasive and highly sensitive bioimaging technique that has shown remarkable strides in plant science. It enables real-time monitoring and analysis of biological and pathological processe...Fluorescence imaging is a non-invasive and highly sensitive bioimaging technique that has shown remarkable strides in plant science. It enables real-time monitoring and analysis of biological and pathological processes in plants by labeling specific molecular or cellular structures with fluorescent probes. However, tissue scattering and phytochrome interference have been obstacles for conventional fluorescence imaging of plants in the ultraviolet and visible spectrum, resulting in unsatisfactory imaging quality. Fortunately, advances in near-infrared(NIR) fluorescence imaging technology(650-900 nm) offer superior spatial-temporal resolution and reduced tissue scattering, which is sure to improve plant imaging quality. In this review, we summarize recent progress in the development of NIR fluorescence imaging probes and their applications for in vivo plant imaging and the identification of plant-related biomolecules. We hope this review provides a new perspective for plant science research and highlights NIR fluorescence imaging as a powerful tool for analyzing plant physiology, adaptive mechanisms, and coping with environmental stress in the near future.展开更多
In recent years, a large amount of research work on thermal property has been done and great achievements have been made in China. We developed a mini thermal conductivity probe. This probe has the advantages of conve...In recent years, a large amount of research work on thermal property has been done and great achievements have been made in China. We developed a mini thermal conductivity probe. This probe has the advantages of convenient operation, short measuring time and small water removal. The measuring results of pig meat and some other organs are satisfactory. The probe can also be applied to the measuring of fruits and vegetables.展开更多
Dextran coated magnetic nanocomposites were prepared. A periodate oxidation method was used to further activate the magnetic dextran, forming magnetic polyaldehyde dextran, which could be conjugated to biomolecules, a...Dextran coated magnetic nanocomposites were prepared. A periodate oxidation method was used to further activate the magnetic dextran, forming magnetic polyaldehyde dextran, which could be conjugated to biomolecules, antibodes. The magnetic labeled nano scaled bioconjugates could be well blocked with BSA and exhibit high activity by ELISA and immunochromatographic strip assay.展开更多
不依赖于纯培养的分子生物技术已广泛应用于环境微生物的研究.综述了近年来在荧光原位杂交技术(Fluorescent in situ hybridization,FISH)和聚合酶链式反应技术(Polymerasechainreaction,PCR)基础上发展起来的几种新分子生物技术的基本...不依赖于纯培养的分子生物技术已广泛应用于环境微生物的研究.综述了近年来在荧光原位杂交技术(Fluorescent in situ hybridization,FISH)和聚合酶链式反应技术(Polymerasechainreaction,PCR)基础上发展起来的几种新分子生物技术的基本原理,包括FISH-MAR、FISH-microelectrodes、Clone-FISH、SIP、PCR-DGGE-cloning-sequencing、PCR-T-RFLP、Real-time fluorescent quantitative PCR和RT-PCR,以及其在污水生物处理系统内硝化菌群研究领域中的应用现状.通过这些分子生物技术,可以识别污水生物处理系统内的硝化菌群;建立硝化菌群动态变化与工艺运行参数之间的相关关系;从微生物学角度对系统运行状态给予最直接、最可靠的分析与证明,为污水生物处理系统的长期稳定运行奠定理论基础.最后对该领域的未来发展进行了展望.展开更多
基金Supported by National Natural Science Foundation of China(Nos.81271516 and 81371625)Program of Shanghai Science and Technology Commission(Nos.13JC1401503 and 14DZ1930402)+1 种基金Shanghai Municipal Health and Family Planning Commission(No.2013313)Exchange Programme Foundation of Doctoral Student under the Office for Graduate Medical Education,Fudan University
文摘Reliable and non-invasive diagnostic tools are highly valuable for successful therapeutic strategies for the treatment of Alzheimer's disease(AD). The existence of neurofibrillary tangles(NFTs) consisting of tau protein are one kind of the pathological features of AD, and its level of severity is correlated with the stage of AD.However, no clinically approved positron emission tomography(PET) probe is currently available for selective imaging of neurofibrillary tangles on patients. In this paper, we report our studies on biological characteristics of [18F]-THK523 as a novel tau imaging probe. With low molecular weight, [18F]-THK523 is stable, electrically neutral, lipophilic and non-mass concentration-dependent. Preliminary biological studies have shown the excellent properties of [18F]-THK523 as brain imaging tracer for further research.
基金supported by the National Natural Science Foundation of China(U20A2038,22022404,21977036,22074050)the CRI Project of National Research Foundation of Republic of Korea(2018R1A3B1052702)+1 种基金Department of Biotechnology,New Delhi,India,for prestigious DBT-Ramalingaswami fellowship(BT/RLF/Re-entry/59/2018)Science&Engineering Research Board,New Delhi(CRG/2021/002476)。
文摘Fluorescence imaging is a non-invasive and highly sensitive bioimaging technique that has shown remarkable strides in plant science. It enables real-time monitoring and analysis of biological and pathological processes in plants by labeling specific molecular or cellular structures with fluorescent probes. However, tissue scattering and phytochrome interference have been obstacles for conventional fluorescence imaging of plants in the ultraviolet and visible spectrum, resulting in unsatisfactory imaging quality. Fortunately, advances in near-infrared(NIR) fluorescence imaging technology(650-900 nm) offer superior spatial-temporal resolution and reduced tissue scattering, which is sure to improve plant imaging quality. In this review, we summarize recent progress in the development of NIR fluorescence imaging probes and their applications for in vivo plant imaging and the identification of plant-related biomolecules. We hope this review provides a new perspective for plant science research and highlights NIR fluorescence imaging as a powerful tool for analyzing plant physiology, adaptive mechanisms, and coping with environmental stress in the near future.
文摘In recent years, a large amount of research work on thermal property has been done and great achievements have been made in China. We developed a mini thermal conductivity probe. This probe has the advantages of convenient operation, short measuring time and small water removal. The measuring results of pig meat and some other organs are satisfactory. The probe can also be applied to the measuring of fruits and vegetables.
文摘Dextran coated magnetic nanocomposites were prepared. A periodate oxidation method was used to further activate the magnetic dextran, forming magnetic polyaldehyde dextran, which could be conjugated to biomolecules, antibodes. The magnetic labeled nano scaled bioconjugates could be well blocked with BSA and exhibit high activity by ELISA and immunochromatographic strip assay.
