Conventional and microwave irradiated synthesis,biological activity evaluation and molecular docking studies of highly substituted piperazine-azole hybrids

Azole derivatives（3,6） obtained starting from 1-（2-methoxyphenyl） piperazine were converted to the corresponding Mannich bases containing β-lactame or flouroquinolone core via a one pot three component reaction.The synthesis of conazole analogues was carried out starting from triazoles by three steps.Reactions were carried out under conventional and microwave mediated conditions.All the newly synthesized compounds were screened for their antimicrobial,enzyme inhibition and antioxidant activity,and most of them displayed good-moderate activity.Binding affinities and non-covalent interactions between enzyme-ligand complexes were predicted with molecular docking method at molecular level.Docking results complemented well the experimental results on α-glucosidase and urease inhibitory effects of the compounds.Higher binding affinities and much more interaction networks were observed for active compounds in contrary to inactive ones.It was predicted with the docking studies that triazole and anisole moieties in the structure of the synthesized compounds contributed to the stabilization of corresponding enzymes through noncovalent interactions.

Surface roughness and its role in mediating cell adhesion on cobalt-chromium-molybdenum alloys

Co-Cr-Mo ally(CCM)is commonly used for orthopaedic and dental implants due to its excellent mechanical properties and corrosion resistance.However,the influence of surface roughness on cell attachment and proliferation remains unclear.This study aimed to elucidate the impact of surface roughness of CCM on the attachment and proliferation of osteoblasts.CCM samples with different values of surface rouges were prepared by polishing.MC3T3-E1 mouse osteoblasts were used for cell culture experiments.Cell attachment,morphology,and the expression of actin stress fibres,vinculin,and distri-bution of yes-associated protein were analysed.Our results suggest that surface rough-ness does not significantly affect cell attachment and proliferation on CCM,unlike on titanium.Thus implies that other properties of CCM,such as physicochemical properties,may play a more substantial role in modulating cell behaviour.This study provides important insights into the design of CCM implants,suggesting that approaches beyond tuning surface roughness may be necessary to improve biocompatibility and osseointegration.