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Animal Safety Test of Bacillus thuringiensis BT Protein
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作者 Weimeng NING Dingkuo LIU +6 位作者 Fang LIU Yuan LI Xiaowei YANG Zhenguo GUAN Guijun YANG Bo ZHANG Pengcheng HU 《Asian Agricultural Research》 2024年第2期31-32,35,共3页
[Objectives]To determine the biological safety of BT protein from Bacillus thuringiensis(Bt)fermentation broth to mammals at high doses.[Methods]Healthy mice were randomly divided into 4 groups with 10 mice in each gr... [Objectives]To determine the biological safety of BT protein from Bacillus thuringiensis(Bt)fermentation broth to mammals at high doses.[Methods]Healthy mice were randomly divided into 4 groups with 10 mice in each group.The experimental groups were fed with Bt fermentation supernatant at 0.2,0.6 and 1.0 mL/kg,respectively,once a day for 7 consecutive days.The blank control group was fed normally without intragastric administration.[Results]There was no significant difference in blood routine and blood biochemical analysis between the experimental group and the control group.After intragastric administration,the mice were dissected,and no obvious pathological changes were found;the heart,liver,spleen,lung and kidney were taken to make tissue sections,and no pathological changes were found by microscopic observation.[Conclusions]Mice can tolerate high doses of BT protein from B.thuringiensis fermentation broth. 展开更多
关键词 Bacillus thuringiensis(Bt) BT protein biological safety
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Biological Safety Evaluation of White Insect Tea in Guizhou 被引量:2
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作者 杨立昌 乙引 《Plant Diseases and Pests》 CAS 2010年第2期55-57,62,共4页
[Objective] The biological safety of white insect tea in Xifeng County of Guizhou Province was evaluated in order to provide a scientific basis for the development of white insect tea. [Method] Acute toxicity test in ... [Objective] The biological safety of white insect tea in Xifeng County of Guizhou Province was evaluated in order to provide a scientific basis for the development of white insect tea. [Method] Acute toxicity test in mice, Ames microbiological test and mouse bone marrow polychromatic erythrocyte micronucleus test were done with wild white inset tea produced in Xifeng County. At the same time, the contents of six kinds of limit pollutants were determined, including Pb,Cr,Cd,Hg,As,and F-. [Result] The results showed that white insect tea had LD505.0 g/kg(BW)in acute toxicity test, negative response in Ames test and microkernel test. In addition, the contents of Pb,Cr,Cd,Hg,As,and F- in the white inset tea were 2.20,1.06,0.46,0.09,0.71,21.50 mg/kg, much less than the national standard of tea (GB2762-2005,NY 659-2003). [Conclusion] The white insect tea produced in Xifeng County of Guizhou Province is non-toxic, and good for human as beverage. 展开更多
关键词 White insect tea biological safety TOXICOLOGY EVALUATION
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Spinal cord biological safety of image-guided radiation therapy versus conventional radiation therapy 被引量:23
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作者 Wanlong Xu Xilinbaoleri +2 位作者 Hao Liu Ruozheng Wang Jingping Bai 《Neural Regeneration Research》 SCIE CAS CSCD 2012年第35期2755-2760,共6页
Tumor models were simulated in purebred Beagles at the T9-10 levels of the spinal cord and treated with spinal image-guided radiation therapy or conventional radiation therapy with 50 or 70 Gy total radiation. Three m... Tumor models were simulated in purebred Beagles at the T9-10 levels of the spinal cord and treated with spinal image-guided radiation therapy or conventional radiation therapy with 50 or 70 Gy total radiation. Three months after radiation, neuronal injury at the T9-10 levels was observed, including reversible injury induced by spinal image-guided radiation therapy and apoptosis induced by conventional radiation therapy. The number of apoptotic cells and expression of the proapoptotic protein Fas were significantly reduced, but expression of the anti-apoptotic protein heat shock protein 70 was significantly increased after image-guided radiation therapy compared with the conventional method of the same radiation dose. Moreover, the spinal cord cell apoptotic index positively correlated with the ratio of Fas/heat shock protein 70. These findings indicate that 3 months of radiation therapy can induce a late response in the spinal cord to radiation therapy; image-guided radiation therapy is safer and results in less neuronal injury compared with conventional radiation therapy. 展开更多
关键词 Image-guided radiation therapy conventional radiation therapy spinal cord NEURONS apoptosis FAS heat shock protein 70 biological safety vertebral body TUMOR
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Research of the Application of Nanometer Materials in Sports Engineering and the Biological Safety 被引量:1
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作者 Lianbo Yang 《International English Education Research》 2014年第7期115-117,共3页
In the modern material engineering, the use of nanometer materials has entered the highly and intensively utilized stage, so new nanometer materials have been continuously found to replace the traditional ordinary mat... In the modern material engineering, the use of nanometer materials has entered the highly and intensively utilized stage, so new nanometer materials have been continuously found to replace the traditional ordinary materials. The so-called nanometer materials have the size within l - 100nm in thickness, which originates from the 1980s. At that time, nanometer materials didn't have a proper development due to the economic level. t towever, with the support of science and technology, this technology has undergone tremendous changes in the related fields. There have been increasing expansion in the kinds and the width in use of the nanometer materials, so have the research of nanometer materials. In this paper, we will briefly analyze the application ofnanometer materials in the sports engineering. 展开更多
关键词 nanometer materials Sports engineering biological safety
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National Standard of "General Requeston Biological Safety" Was Officially Issued
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《China Standardization》 2004年第5期30-30,共1页
关键词 National Standard of General Requeston biological safety Was Officially Issued
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Emerging role of dual biologic therapy for the treatment of inflammatory bowel disease 被引量:1
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作者 Matthew D McCormack Natasha A Wahedna +1 位作者 David Aldulaimi Peter Hawker 《World Journal of Clinical Cases》 SCIE 2023年第12期2621-2630,共10页
Biologic agents have now been used in the management of inflammatory bowel disease(IBD)for many years where experience,expertise and confidence in their use has developed over time.In the United Kingdom,there are well... Biologic agents have now been used in the management of inflammatory bowel disease(IBD)for many years where experience,expertise and confidence in their use has developed over time.In the United Kingdom,there are well established guidelines and recommendations for both single agent biologic treatments,and with combination therapy of a biologic agent with a small molecule agent in maintenance therapy.In recent times,there has been increasing interest and experience using dual biologic therapy(DBT)in IBD,primarily in difficult to treat and refractory cases with high disease burden.However,published data on use,experience and safety profiles is limited and large-scale studies remain low in number in this developing area.We therefore aim to present a summary and review of the available published data in this area to help us better understand the emerging role of DBT in IBD. 展开更多
关键词 Dual biologic therapy Inflammatory bowel disease Crohn’s disease Ulcerative colitis Combination therapy Biologic safety
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A biological safety evaluation on reclaimed water reused as scenic water using a bioassay battery 被引量:9
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作者 Dongbin Wei Zhuowei Tan Yuguo Du 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2011年第10期1611-1618,共8页
An assessment method based on three toxicity tests (algae growth inhibition, daphnia immobilization and larval fish toxicity) was used to screen the biological safety of reclaimed water which was reused as sole repl... An assessment method based on three toxicity tests (algae growth inhibition, daphnia immobilization and larval fish toxicity) was used to screen the biological safety of reclaimed water which was reused as sole replenishment for scenic water system in a park (SOF Park) in northern China. A total of 24 water samples were collected from six sites of water system in the SOF Park in four different seasons. The results indicated that: (1) the reclaimed water directly discharged from a reclamation treatment plant near the SOF Park as influent of park had relatively low biological safety (all samples were ranked as C or D); (2) the biological safety of reclaimed water was improved greatly with the ecological reclamation treatment processes composing of artificial wetland system and followed oxidation pond system; (3) the biological safety of reclaimed water in the main lake of SOF Park kept at a health status during different seasons (all samples were ranked as A); (4) there was some certain correlation (R2= 0.5737) between the sum of toxicity scores and dissolved organic carbon for the studied water samples. It was concluded that the assessment method was reliable to screen the safety of reclaimed water reused as scenic water, and the reclaimed water with further ecological purification processes such as artificial wetland and oxidation pond system can be safely reused as scenic water in park. 展开更多
关键词 biological safety water quality index BIOASSAY reclaimed water
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Study of biological safety of scaffold material with heterologous deproteinized bone 被引量:12
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作者 刘雷 唐康来 +2 位作者 杨柳 简月奎 李起鸿 《Chinese Journal of Traumatology》 CAS 2006年第4期234-237,共4页
To evaluate the biological safety of manufactured heterologous deproteinized bone and to provide an experimental basis for clinical applications. Methods : Deproteinized bone ( 10 mm) and leaching liquor were made... To evaluate the biological safety of manufactured heterologous deproteinized bone and to provide an experimental basis for clinical applications. Methods : Deproteinized bone ( 10 mm) and leaching liquor were made from pig ribs with a series of physical and chemical methods, then were evaluated through acute and subacute toxicity test, hemolysis test, pyrogen test, intracutaneous test, intramuscular implantation test and cytotoxity test. Results : No obvious toxicity, hemolysis, pyrogenic characteristics, skin irritation, inflammatory reaction after intramusclar implantation and cytotoxity were observed. Conclusions: The heterologous deproteinized bone has good biological safety and meets all the demands of scaffold material for tissue engineering. 展开更多
关键词 Transplantation heterogeneous Tissue engineering biological safety
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Paclitaxel formulation with stable sustained-release behavior and its biological safety evaluation 被引量:2
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作者 LI XuePing ZHAN Qi +8 位作者 QI HongZhao HAN DongLin QIN YaoYao CHEN Ning LONG LiXia ZHAO Jin HOU Xin YUAN XuBo YANG XianJin 《Science China(Technological Sciences)》 SCIE EI CAS CSCD 2019年第7期1151-1159,共9页
Biological safety and stable sustained-release of the drug are two crucial issues involved in the formulation of paclitaxel.Focusing on these issues, by using the FDA approved polylactide as carrier material, soybean ... Biological safety and stable sustained-release of the drug are two crucial issues involved in the formulation of paclitaxel.Focusing on these issues, by using the FDA approved polylactide as carrier material, soybean lecithin as surfactant and maltodextrin as thickener, paclitaxel loaded PLA microspheres were simply prepared by solvent evaporation, thus guaranteeing the biological safety. The introduction of maltodextrin as a thickener aided to a stable sustained-release of paclitaxel. Surface morphology, particle size, drug loading rate, encapsulation efficiency and in vitro drug release behavior were investigated.Biological safety evaluations such as acute toxicity, allergies, hemolysis, skin stimulation and genotoxicity test were also carried out. Results showed that the obtained microspheres were biocompatible and could release paclitaxel at a desirable constant rate.Therefore, the simply prepared paclitaxel formulation with good biological safety and desirable release behavior exhibited great potential of local injection of paclitaxel for the clinical use in the future. 展开更多
关键词 paclitaxel loaded PLA microsphere stable sustained-release biological safety MALTODEXTRIN solvent evaporation clinical paclitaxel formulation
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Genomovars of Burkholderia cepacia Complex from Rice Rhizosphere and Clinic in China
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作者 Lou Miao miao ZHANG Li xin Su Ting XIE Guan lin 《Rice science》 SCIE 2007年第3期229-234,共6页
Burkholderia cepacia is regarded as a genetically distinct but phenotypically similar bacteria group referring to Burkholderia cepacia complex (Bcc), which is found not only in clinic but also in rice growing enviro... Burkholderia cepacia is regarded as a genetically distinct but phenotypically similar bacteria group referring to Burkholderia cepacia complex (Bcc), which is found not only in clinic but also in rice growing environment. It is very important in microbial safety of rice for us to understand the genomovar status of Bcc. Genomovar analysis was performed among 87 Bcc isolates by means of Hae Ⅲ-recA RFLP assays and species-specific PCR tests. Three genomovars were found from the rice rhizosphere including Ⅰ, ⅢB and V, and genomovar V was predominant. Genomovars Ⅰ, ⅢA and ⅢB existed in the clinical samples, and genomovar ⅢA was the most popular. It showed that genomovar composition was different between the Bcc strains from the rice rhizosphere and clinical environment. Simultaneously, the results revealed the genetic diversity of Bcc strains from the rice rhizosphere, and genomovar Ⅲ referred as virulent species in clinic also existed in the rice rhizosphere. 展开更多
关键词 Burkholderia cepacia complex rice rhizosphere CLINIC GENOMOVARS DIVERSITY biological safety biological control risk assessment
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Development of a large-scale isolation chamber system for the safe and humane care of medium-sized laboratory animals harboring infectious diseases 被引量:8
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作者 Xin PAN Jian-cheng QI Ming LONG Hao LIANG Xiao CHEN Han LI Guang-bo LI Hao ZHENG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2010年第10期771-778,共8页
The close phylogenetic relationship between humans and non-human primates makes non-human primates an irreplaceable model for the study of human infectious diseases.In this study,we describe the development of a large... The close phylogenetic relationship between humans and non-human primates makes non-human primates an irreplaceable model for the study of human infectious diseases.In this study,we describe the development of a large-scale automatic multi-functional isolation chamber for use with medium-sized laboratory animals carrying infectious diseases.The isolation chamber,including the transfer chain,disinfection chain,negative air pressure isolation system,animal welfare system,and the automated system,is designed to meet all biological safety standards.To create an internal chamber environment that is completely isolated from the exterior,variable frequency drive blowers are used in the air-intake and air-exhaust system,precisely controlling the filtered air flow and providing an air-barrier protection.A double door transfer port is used to transfer material between the interior of the isolation chamber and the outside.A peracetic acid sterilizer and its associated pipeline allow for complete disinfection of the isolation chamber.All of the isolation chamber parameters can be automatically controlled by a programmable computerized menu,allowing for work with different animals in different-sized cages depending on the research project.The large-scale multi-functional isolation chamber provides a useful and safe system for working with infectious medium-sized laboratory animals in high-level bio-safety laboratories. 展开更多
关键词 Multi-function Medium-sized animal Isolation chamber biological safety
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Biosafety and biobanking: Current understanding and knowledge gaps 被引量:4
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作者 Julie Roux Maissa Zeghidi +1 位作者 Stephanie Villar Zisis Kozlakidis 《Biosafety and Health》 CSCD 2021年第5期244-248,共5页
Infectious disease outbreaks,such as'Coronavirus disease 20190(COVID-19),can constitute major global health threats with far-reaching consequences.As outbreaks develop,the international scientific community must p... Infectious disease outbreaks,such as'Coronavirus disease 20190(COVID-19),can constitute major global health threats with far-reaching consequences.As outbreaks develop,the international scientific community must provide high-quality scientific research-ready biological samples to solve the existing clinical and epidemiological questions to better combat the pandemic.Such examples are provided by dedicated biobank facilities,the latter collecting increasingly high volumes of biological samples.However,the more significant concentrations of infectious or potentially infectious biological materials can create a safety risk.The current short report describes the first attempt to identify the published scientific works on biobanking and safety.Three broad thematic areas have been identified:the physical security relevant to staff and sample integrity,the data safety aspects,and the governance parameters relating to the previous two.While the current publications reflect a broad alignment with existing standards and best practices in the biobanking field,they also demonstrate an opportunity for further in-depth work on this field in the post-COVID-19 era. 展开更多
关键词 BIOsafety BIOBANK Data safety biological safety safety regulations
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The role of leaders in laboratory safety: With an example from industry
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作者 David R.Franz 《Biosafety and Health》 2019年第1期4-5,共2页
Infectious disease research,like any scientific enterprise,is dependent on technology,subject matter experts,hard work…and engaged leadership.The pace of progress in research and development of vaccines,drugs,diagnos... Infectious disease research,like any scientific enterprise,is dependent on technology,subject matter experts,hard work…and engaged leadership.The pace of progress in research and development of vaccines,drugs,diagnostics or foundational knowledge is always more rapid when laboratory organizational cultures are collaborative and communication is open.Relationships of trust between individual scientists and with their leaders in an organization further fuel productivity.Laboratory safety is sometimes seen as just another administrative function,but that is far from the case.A focus on safety in an infectious disease laboratory not only protects the staff and the community around the lab;it also contributes to a healthy and vibrant organizational culture and even enhances productivity. 展开更多
关键词 High-containment laboratory biological safety LEADERSHIP Organizational culture
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Surface charge-convertible quaternary ammonium salt-based micelles for in vivo infection therapy 被引量:1
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作者 Dengfeng He Yifeng Tan +6 位作者 Pengfei Li Yadong Luo Yuhong Zhu Yunlong Yu Jiali Chen Ning Ning Shiyong Zhang 《Chinese Chemical Letters》 SCIE CAS CSCD 2021年第5期1743-1746,共4页
Quaternary ammonium salts(QASs) are excellent candidates for treating stubborn bacterial infections caused by biofilms due to their high sterilization efficiency and potential inhibition of the development of drug res... Quaternary ammonium salts(QASs) are excellent candidates for treating stubborn bacterial infections caused by biofilms due to their high sterilization efficiency and potential inhibition of the development of drug resistance.However,the inherent toxicity of QASs,including cytotoxicity,protein absorption and hemolysis,severely limits their applications in vivo.Herein,a charge-co nvertible quaternary ammonium salt-based micelle(QAS-SL@CM) was constructed by co-assembly of two amphiphiles with opposite charges and shell cross-linking strategy.The toxicity of the QAS-SL@CM could be greatly reduced towa rds human cells contrast to the corresponding QASs.By response to the acidic environment at infection sites,the surface charge of QAS-SL@CM could be immediately changed to positive and then target to negatively charged bacteria.Furthermo re,β-thiopropionate bonds on QAS-SL@CM could also be disintegrated under acid environment to release QASs to kill bacteria.Importantly,the QAS-SL@CM showed significant therapeutic effect in mice subcutaneous abscesses models without interference with normal cells.Therefore,a surface adaptive micelle constructed by charge-convertible strategy has been developed to ove rcome the cytotoxicity of QAS s,and could intelligently respond to the microenvironment of infected wound for in vivo infection therapy,which shows promising application in clinic. 展开更多
关键词 Quaternary ammonium salt Co-assembled micelle BIOFILM biological safety In vivo infection
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