Use of biologically synthesized antimicrobial nanoparticles for improving peritoneal dialysis technique： a translational research perspective

Peritoneal dialysis （PD） is a well-established renal replacement therapy （RRT） for end-stage renal failure （ESRF） and offers certain clear advantages over hemodialysis. However, PD is often associated with a high risk of infection of the intraperitoneal cavity, subcutaneous tunnel and catheter exit site, which may subsequently form microbial biofilms.

An Efficient Numerical Scheme for Biological Models in the Frame of Bernoulli Wavelets

This article considers three types of biological systems:the dengue fever disease model,the COVID-19 virus model,and the transmission of Tuberculosis model.The new technique of creating the integration matrix for the Bernoulli wavelets is applied.Also,the novel method proposed in this paper is called the Bernoulli wavelet collocation scheme(BWCM).All three models are in the form system of coupled ordinary differential equations without an exact solution.These systems are converted into a system of algebraic equations using the Bernoulli wavelet collocation scheme.The numerical wave distributions of these governing models are obtained by solving the algebraic equations via the Newton-Raphson method.The results obtained from the developed strategy are compared to several schemes such as the Runge Kutta method,and ND solver in mathematical software.The convergence analyses are discussed through theorems.The newly implemented Bernoulli wavelet method improves the accuracy and converges when it is compared with the existing methods in the literature.

Application of LC–MS/MS for quantitative analysis of glucocorticoids and stimulants in biological fuids

Liquid chromatography tandem mass chromatography （LC-MS/MS） is an important hyphenated technique for quantitative analysis of drugs in biological fluids. Because of high sensitivity and selectivity, LC-MS/MS has been used for pharmacokinetic studies, metabolites identification in the plasma and urine. This manuscript gives comprehensive analytical review, focusing on chromatographic separation approaches （column packing materials, column length and mobile phase） as well as different acquisition modes （SIM, MRM） for quantitative analysis of glucocorticoids and stimulants. This review is not meant to be exhaustive but rather to provide a general overview for detection and confirmation of target drugs using LC-MS/MS and thus useful in the doping analysis, toxicological studies as well as in pharmaceutical analysis.

Synthesis, Characterizations, Biological, and Molecular Docking Studies of Some Amino Acid Schiff Bases with Their Cobalt(II) Complexes

The synthesis and structural characterization of cobalt(II) complexes of amino acid Schiff bases was prepared from Salicylaldehyde and three amino acid (Valine, Leucine, and Isoleucine)?in basic medium. The metal complexes was synthesized by treating an ethanolic solution of the ligand with appropriate amount of metal salts [1:2] [M:L] ratio. The synthesized Schiff bases and their metal complexes have been investigated on the bases of elemental chemical analysis, FTIR, electronic spectral,?1HNMR,?13CNMR, MS, molar conductance and magnetic susceptibility measurements. The electronic spectra of the metal complexes and their magnetic susceptibility measurements suggest octahedral structures are the probable coordination geometries for the isolated complexes. The Schiff bases and their metal complexes were preliminary scanned against various strains of microbes to study their biological effect.

Damage Characteristics and Control Techniques of Longhorn Beetles in Coffee Garden

Coffee longhorn beetles are major trunk-boring pests of coffee trees,which are widely distributed in most areas of China.The biological characteristics,damage characteristics,life history and control techniques of two species of coffee longhorn beetles are reported in this paper.

Current and future of anterior cruciate ligament reconstruction techniques

In recent years,anterior cruciate ligament(ACL)reconstruction has generally yielded favorable outcomes.However,ACL reconstruction has not provided satisfactory results in terms of the rate of returning to sports and prevention of osteoarthritis(OA)progression.In this paper,we outline current techniques for ACL reconstruction such as graft materials,double-bundle or single-bundle reconstruction,femoral tunnel drilling,all-inside technique,graft fixation,preservation of remnant,anterolateral ligament reconstruction,ACL repair,revision surgery,treatment for ACL injury with OA and problems,and discuss expected future trends.To enable many more orthopedic surgeons to achieve excellent ACL reconstruction outcomes with less invasive surgery,further studies aimed at improving surgical techniques are warranted.Further development of biological augmentation and robotic surgery technologies for ACL reconstruction is also required.

毛花猕猴桃新品种“赣绿1号”的生物学特性及关键栽培技术

以毛花猕猴桃“赣绿1号”为研究材料,“赣猕6号”(相似品种)为试验对照,对其主要生物学特性进行了系统观测与分析,探明该品种的生长发育规律,以期为制定合理的栽培管理措施提供理论依据。研究表明,在江西省宜春市奉新县地区,“赣绿1号”伤流期在2月上中旬,初花期在5月上旬,终花期在5月中旬,果实成熟期在10月下旬,为晚熟二倍体品种;“赣绿1号”成叶长卵圆形,叶面无茸毛,花为伞房花序,每花序单花3~5朵,花瓣粉红色,花药淡黄色;“赣绿1号”果实近圆柱形至长卵圆形,平均单果质量46.5 g,果形指数为2.52,果实软熟时可溶性固形物含量19.40%,最高达22.20%,干物质含量21.75%,可溶性糖含量为9.25%,可滴定酸含量为0.94%,抗坏血酸含量为662.6 mg/100 g,果肉呈墨绿色,肉质细嫩,风味甜香。“赣绿1号”植株树势较强壮,耐热抗旱,适应性强,果实富含抗坏血酸,易剥皮,综合性状优良且遗传性状稳定。“赣绿1号”在无霜期130 d以上、≥10℃积温达1500℃以上的地方均可栽培,宜选择海拔1000 m以下,pH值6.0~7.0,土壤疏松肥沃、有机质含量丰富、灌溉方便的砂壤土建园。成龄果园一般每年施肥2~3次,萌芽肥以N肥为主,壮果肥以P、K、Ca肥为主,秋季基肥以有机肥为主。架式宜采用水平大棚架或T型小棚架,株行距(2.5~3.0)m×(3.5~4.0)m,雌雄株比例为(6~8)∶1。蕾期及时“零芽”折梢修剪,幼果期及时疏果与套袋。采用单干双主蔓多侧蔓整形方式,冬季修剪时可采用中长蔓修剪法,短截、疏删与回缩相结合。

我国黄毛鼠研究进展

黄毛鼠Rattus losea是分布在中国长江以南地区以及东南亚地区的重要农田害鼠。该鼠栖息地广,食性杂,盗食各种农作物,还是某些疾病的宿主和传播者。本文从形态特征、种群遗传学、生物学特性、种群生态学、抗药性、防治技术等方面详细介绍了黄毛鼠的研究进展。并根据目前的研究现状和存在问题,提出建立基于精准预测预报,研发生态优先、环境友好的防控新技术、新产品为核心的综合防控技术体系,以期为黄毛鼠的可持续治理提供参考。

生物导向型预备技术在口腔修复中的应用

口腔修复中的牙体预备边缘一直是口腔医生关注的热点问题,不当的预备边缘可能导致龋病和牙周炎等疾病,最终导致修复失败。生物导向型预备技术的应用已证明可恢复良好的牙周软硬组织形态,有望替代传统牙体预备方法。从生物导向型预备技术在贴面、全冠、种植中的应用综合论述生物导向型预备技术在口腔修复中的应用。

牛羊布鲁氏菌病的诊断及综合防控措施探究

布鲁氏菌病是由布鲁氏菌引起的一种人畜共患传染病,该病会引起家畜繁殖障碍、关节炎等症状。布鲁氏菌病传播速度快,范围广,会影响牛羊的健康生长,进而对我国养殖经济造成一定危害。文章从布鲁氏菌病的病原特点、诊断技术和防治对策等方面进行概述,以期为探究有效的防控布鲁氏菌病的方法以及牛羊健康养殖提供参考。

生物导向性预备技术用于美学区残冠及二次修复1例

本文报道1例在上前牙的残冠修复及二次修复中应用生物导向性预备技术(biologically oriented preparation technique,BOPT)来实现牙体缺损的修复以及软组织塑形的病例,BOPT技术在前牙美学区牙体预备中的应用,取得了良好的美学效果。

“吉夫纳”番荔枝生物学特性及栽培技术

“吉夫纳”作为当前广西番荔枝产业发展的主要品种,其种植效益尤为突出。通过多年观察该品种的植物学特性、生长结果习性、果实经济性状和物候期等特性,发现其在广西种植区的综合表现优良,具体表现为:早结,定植2年即可投产;丰产,每667 m2平均产量可达1151.7 kg;优质,可溶性固形物含量为21.68%、维生素C含量为32.40 mg/100 g、K含量为284.82 mg/100 g、Ca含量为25.02 mg/100 g;产期易调节,4月~10月修剪即可成花。并探索总结出该品种在高标准建园、强化水肥管理、科学产期调节、精细花果管理和综合防控病虫害等栽培领域上的技术要点,以期为“吉夫纳”在广西适栽区的推广应用提供理论参考。

Identification of Motor Nuclei in the Medulla Oblongata of Carp for Biological Control

Brain-controlled technology is the key technology in biological control,and the corresponding relationship between animal brain nuclei and motor behavior is the core.The purpose of this study was to explore the motor nuclei of the medulla oblongata in carp.The carps were subjected to electrical stimulation and chemical stimulation experiments,respectively,in the water-free state,and the effective chemical stimulation sites were injected with the pontamine sky blue solution.The brain tissue sections were obtained by paraffin tissue section technology and the neutral red staining method.By comparing the positions of the brain nuclei shown in earlier studies,the motor nerve nuclei in the medulla oblongata were identified.The brain electrode was implanted into the motor nucleus of the medulla oblongata,and the underwater control experiment and behavioral tests were carried out with different electrical stimulation parameters.The results showed that the abducens nucleus(NVI)was the motor nucleus that controls the ipsilateral steering,and the facial nucleus(NVII)was the motor nucleus that controls the forward movement.By adjusting the stimulation voltage and the stimulation pulse number,the carp can be stably controlled to achieve a left–right steering motion of 30°–180°and a forward motion of more than 80 cm/s.This study indicated that the quantitative control of the steering and forward behavior of the carp can be achieved by electrical stimulation of the NVI and NVII,which provided a certain experimental basis for the accurate control of the carp robot.

复杂基质样品中金属纳米颗粒的SP-ICP-MS分析方法及应用研究进展

近年来,纳米技术和纳米材料的大规模应用导致大量的金属纳米颗粒(MNPs)释放并积累到环境中。由于独特的物理化学特性及生物毒性,环境中的MNPs严重威胁到生态环境及人类健康。因此,对复杂基质样品(土壤、沉积物、水体、生物)中的MNPs进行表征具有重要意义。在已报道的各种表征MNPs的技术中,单颗粒电感耦合等离子体质谱(SP-ICP-MS)在分离、鉴定和定量复杂基质样品中的MNPs方面发挥了重要作用。该文主要介绍了SP-ICP-MS分析技术的原理及其复杂基质的样品前处理方法,评述了近年来SP-ICP-MS在环境科学、生命科学、医学MNPs分析应用中的新进展,并提出了SP-ICP-MS技术未来研究和应用的方向。

‘黑金刚’黄皮在南宁市良庆区的表现及栽培技术

为了研究‘黑金刚’黄皮在广西南宁市良庆区的生长表现情况,对‘黑金刚’黄皮物候期、植物学性状及果实性状进行观测和评价。结果表明,该品种综合性状表现突出:3月中旬始花,8月中旬果实成熟;平均果穗质量323.5 g,果实中等大(单粒果质量平均8.1 g),单粒果实长圆形或近圆形(果实平均横径2.1 cm、纵径2.6 cm),成熟时果皮呈古铜色,完熟时果皮变黑褐色,果实可溶性固形物含量16.5%,可食率60.7%,每粒单果含种子1~2粒,果实清甜芳香,肉质结实柔滑,多汁无渣。总的来说,该品种有特色、品质优,能较好地迎合产销市场的需求,具有较广阔的发展前景,适合在广西南宁地区推广种植。

核化生应急救援关键护理技术培训平台的设计及应用

目的:设计一种核化生应急救援关键护理技术培训平台,以为核化生应急救援护理人员提供专业的技术培训。方法:该平台采用浏览器/服务器(Browser/Server,B/S)架构进行设计,服务端采用C#语言开发,用户界面采用HTML5技术搭建,交互逻辑通过JavaScript语言编写。整个平台包括视频课堂、课后测试和模拟训练3个功能模块。结果:该平台能够实现核化生应急救援关键护理技术(包括理论和操作)的培训及考核,且不受时间、空间、师资等因素的限制,有利于提高培训效果。结论:该平台能够满足核化生应急救援护理关键技术培训的需求,为核化生应急救援护理人员的培训提供了有力的工具。

生物导向型牙体预备技术对修复体边缘牙周菌斑定植水平和龈沟液炎症因子水平的影响

目的:探讨运用生物导向型预备技术制备的修复体边缘牙周菌斑定植和龈沟液(GCF)炎症因子水平及临床意义。方法:纳入102例慢性牙周炎患者,随机分为2组(n=51),对照组行传统牙体预备,观察组行生物导向性预备技术。比较两组患者术前和术后6月修复体边缘骨吸收量、探诊方面的具体深度、牙龈乳头以及改良菌斑方面的具体指数、改良龈沟出血指数,在修复体位置采集龈沟液,测定TNF-α、 IL-6和MMP8水平。结果:术后6月观察组和对照组患者近中、远中及平均骨吸收量差异无统计学意义(P>0.05)。两组患者修复体牙周指数明显降低(P<0.05),且观察组患者改良龈沟出血指数、探诊深度、牙龈乳头指数和改良菌斑指数明显低于对照组(P<0.05)。两组患者龈沟液炎性因子明显降低,且观察组患者TNF-α、MMP8和IL-6明显低于对照组(P<0.05)。观察组患者舒适度、固位功能、美观度和总分明显高于对照组(P<0.05),而在咀嚼功能、语言功能方面差异未见统计学意义(P>0.05)。结论:生物导向性预备技术应用于慢性牙周炎患者,可有效改善修复体定植水平,消除龈沟液炎症反应,提高患者满意度。

颐和园桃红颈天牛发生规律及绿色防控技术应用试验

桃红颈天牛Aromia bungii Faldermann是危害桃树的主要蛀干害虫,近年危害渐趋严重,对颐和园西堤的“桃红柳绿”和万寿山区域的桃花景观构成了很大威胁。通过虫害调查和采用一系列防治措施,明确颐和园桃红颈天牛发生规律及绿色防控技术的可行性。结果表明,桃红颈天牛在颐和园2~3 a发生1代。探索出了以糖醋液诱杀、人工捕杀、阻隔产卵、生物防治、人工钩杀、局部喷药等措施为主,植物检疫和精细化养护管理为辅的桃红颈天牛绿色防控技术。与对照区比对,连续三年应用该技术对成虫的防治率达89.81%,对幼虫的防治效果最高达80.40%。该绿色防控技术为以颐和园为代表的公园景区实现对桃红颈天牛的绿色防控提供了指导。

肿瘤质子治疗技术及应用进展

质子治疗有显著的剂量学优势,但也面临许多挑战,比如质子束相对生物效应值的不确定性及质子治疗中对位置要求的敏感性等问题。质子的物理剂量学优势能否充分转化为患者的临床获益仍然存在一定的争议。近年来的临床研究初步展现了质子治疗在儿童肿瘤、头颈部肿瘤和一些对高能X线不敏感肿瘤中的优势。随着质子技术的发展,迫切需要解决质子临床应用设备成本过高问题,只有发展小型化装置,才能有望加快对质子治疗技术进行普及,质子治疗技术的优化将带来治疗成本的降低和患者的受益。

三门峡市月季桑拟轮蚧发生规律及药剂治理技术研究

研究结果表明,月季桑拟轮蚧在三门峡市1 a发生2代,以受精的雌成虫越冬。翌年4月下旬开始产卵,5月上中旬为产卵盛期。雌雄性比为1∶2.8,平均产卵量86~108粒。雄成虫具群居性、毁灭性危害。天敌昆虫主要有红点唇瓢虫、黑缘红瓢虫、中华圆蚧蚜小蜂等。田间药效试验证明,在1龄若虫期防治效果优于介壳形成期,若虫期防治可选用40%速扑杀乳油600倍液、1.2%苦·烟乳油2500倍液;介壳形成期可选用1.2%苦·烟乳油2500倍液、55%氯氰·毒死蜱乳油1500倍液。