Graphene oxide (GO) is a graphene derivatives that has oxygen-containing functional groups on the graphene basal plane, such as hydroxyl, carbonyl, epoxy and carboxyl groups. GO is considered as a promising material f...Graphene oxide (GO) is a graphene derivatives that has oxygen-containing functional groups on the graphene basal plane, such as hydroxyl, carbonyl, epoxy and carboxyl groups. GO is considered as a promising material for biological applications owing to its excellent surface functionalizability, high specific suface area and good biocompatibility. In this study, GO/chitosan hybrid scaffolds were prepared for tissue engineering. Nano silver was loaded into the scaffold to improve its antibacterial ability and biomimetic Ca-P coatings were deposited on the scaffold surface to enhance its osteoconductivity. First, GO was prepared by the chemical oxidization of graphite. Secondly, nano-Ag loaded GO was prepared by chemical reducing Ag ions in GO solutions. Then, nano-Ag loaded GO solution was mixed with CS solution to form GO-CS gel. Chitosan (CS) and GO were crosslinked by electrostatic interactions between oxygen-containing functional groups of GO and NH2 groups of CS. The gel were freeze dried to produce nano-Ag loaded GO/CS hybrid porous scaffolds. Finally, the as-prepared scaffolds were immersed the into a supersaturated calcium phosphate solution (SCPS) for 7 days to deposite CaP coatings on the surface of the micropores. SEM images showed that nano-Ag uniformly distributed in the scaffold and the CaP covered most of the scaffold surfaces. In vitro cell culture and antimicrobial test indicated the biomimetic mineralized Ag-CS-GO scaffolds have good osteoconductivity and bactericidal ability.展开更多
文摘Graphene oxide (GO) is a graphene derivatives that has oxygen-containing functional groups on the graphene basal plane, such as hydroxyl, carbonyl, epoxy and carboxyl groups. GO is considered as a promising material for biological applications owing to its excellent surface functionalizability, high specific suface area and good biocompatibility. In this study, GO/chitosan hybrid scaffolds were prepared for tissue engineering. Nano silver was loaded into the scaffold to improve its antibacterial ability and biomimetic Ca-P coatings were deposited on the scaffold surface to enhance its osteoconductivity. First, GO was prepared by the chemical oxidization of graphite. Secondly, nano-Ag loaded GO was prepared by chemical reducing Ag ions in GO solutions. Then, nano-Ag loaded GO solution was mixed with CS solution to form GO-CS gel. Chitosan (CS) and GO were crosslinked by electrostatic interactions between oxygen-containing functional groups of GO and NH2 groups of CS. The gel were freeze dried to produce nano-Ag loaded GO/CS hybrid porous scaffolds. Finally, the as-prepared scaffolds were immersed the into a supersaturated calcium phosphate solution (SCPS) for 7 days to deposite CaP coatings on the surface of the micropores. SEM images showed that nano-Ag uniformly distributed in the scaffold and the CaP covered most of the scaffold surfaces. In vitro cell culture and antimicrobial test indicated the biomimetic mineralized Ag-CS-GO scaffolds have good osteoconductivity and bactericidal ability.
