A localized surface plasmon resonance(LSPR) biosensor was prepared with gold nanospheres(Au NSs) coated on the tip face of the optical silica fiber. Au NSs with the sizes of 20 nm and 80 nm were used. The sensitivitie...A localized surface plasmon resonance(LSPR) biosensor was prepared with gold nanospheres(Au NSs) coated on the tip face of the optical silica fiber. Au NSs with the sizes of 20 nm and 80 nm were used. The sensitivities of Au NS_(20 nm) and Au NS_(80 nm) modified sensors to bulk refractive index(RI) variation are 82.86 nm/RIU and 218.98 nm/RIU, respectively. The Au NS_(80 nm) modified sensor was used for the detection of 40 bases DNA hybridization and the limit of detection is 50 nmol/L, where the 40-bases DNA probe was covalently linked with Au NS_(80 nm). The complementary DNA sequence in tris-acetate-EDTA(TAE) buffer solution was detected as the target DNA. This fiber sensor has the advantages of small sample consumption, easy fabrication and high sensitivity.展开更多
基金supported by the National Basic Research Program of China(No.2014M561055)the National High Technology Research and Development Program(No.2012AA101608)the National Natural Science Foundation of China(No.61401433)
文摘A localized surface plasmon resonance(LSPR) biosensor was prepared with gold nanospheres(Au NSs) coated on the tip face of the optical silica fiber. Au NSs with the sizes of 20 nm and 80 nm were used. The sensitivities of Au NS_(20 nm) and Au NS_(80 nm) modified sensors to bulk refractive index(RI) variation are 82.86 nm/RIU and 218.98 nm/RIU, respectively. The Au NS_(80 nm) modified sensor was used for the detection of 40 bases DNA hybridization and the limit of detection is 50 nmol/L, where the 40-bases DNA probe was covalently linked with Au NS_(80 nm). The complementary DNA sequence in tris-acetate-EDTA(TAE) buffer solution was detected as the target DNA. This fiber sensor has the advantages of small sample consumption, easy fabrication and high sensitivity.
