Environmental pollutants,such as bisphenol A(BPA) have recently been implicated in the development of adverse birth outcomes.However,the underlying teratogenic mechanisms remain unclear.We investigated the effects of ...Environmental pollutants,such as bisphenol A(BPA) have recently been implicated in the development of adverse birth outcomes.However,the underlying teratogenic mechanisms remain unclear.We investigated the effects of BPA on the migration and invasion of human primary extravillous trophoblast HTR-8/SVneo cells.Our results indicated that BPA reduced cell migration and invasion.Moreover,it altered the ratio of matrix metalloproteinases(MMPs) and tissue inhibitors of MMPs(TIMPs) by downregulating MMP-2 and MMP-9,and upregulating TIMP-1 and TIMP-2.Furthermore,BPA suppressed integrin β1,integrin α5,and vimentin.Interestingly,BPA-induced invasion was partially restored by G15,a membrane G-protein-coupled estrogen receptor 30 antagonist.We further revealed that 42 proteins were differentially expressed by mass spectrometry analysis,which could be divided into three categories based on gene ontology including biological process,cellular component,and molecular function.These results suggest that BPA reduces HTR-8/SVneo cell migration and invasion by downregulating MMP-2 and MMP-9,up-regulating TIMP-1 and TIMP-2,and suppressing adhesion molecules.展开更多
Bisphenol A(BPA)and replacements,such as bisphenol S(BPS)and F(BPF)and other substitutes(BPSMAE,D-8 and TGSA)have been recently reported in thermal paper.In this study,BPA,BPS,BPF;BPS-MAE,D-8 and TGSA were analysed in...Bisphenol A(BPA)and replacements,such as bisphenol S(BPS)and F(BPF)and other substitutes(BPSMAE,D-8 and TGSA)have been recently reported in thermal paper.In this study,BPA,BPS,BPF;BPS-MAE,D-8 and TGSA were analysed in indoor dust collected in Spain from different microenvironments(living rooms,bedrooms,cars and offices).Levels were compared with those reported in our previous study(shops and other public microenvironments,where frequent use of thermal paper is expected).We investigated the differences between microenvironments,possible correlations between compounds and estimated the ingestion rates.BPS-MAE,D-8 and TGSA were present at levels up to 529,128 and 356 ng g^-1,respectively,in all the microenvironments.The highest levels were measured in cars.展开更多
基金supported by the National Basic Research Program of China(973 Program,No.2014CB943303)。
文摘Environmental pollutants,such as bisphenol A(BPA) have recently been implicated in the development of adverse birth outcomes.However,the underlying teratogenic mechanisms remain unclear.We investigated the effects of BPA on the migration and invasion of human primary extravillous trophoblast HTR-8/SVneo cells.Our results indicated that BPA reduced cell migration and invasion.Moreover,it altered the ratio of matrix metalloproteinases(MMPs) and tissue inhibitors of MMPs(TIMPs) by downregulating MMP-2 and MMP-9,and upregulating TIMP-1 and TIMP-2.Furthermore,BPA suppressed integrin β1,integrin α5,and vimentin.Interestingly,BPA-induced invasion was partially restored by G15,a membrane G-protein-coupled estrogen receptor 30 antagonist.We further revealed that 42 proteins were differentially expressed by mass spectrometry analysis,which could be divided into three categories based on gene ontology including biological process,cellular component,and molecular function.These results suggest that BPA reduces HTR-8/SVneo cell migration and invasion by downregulating MMP-2 and MMP-9,up-regulating TIMP-1 and TIMP-2,and suppressing adhesion molecules.
基金Authors gratefully acknowledge financial support from Spanish Ministry of Science,Innovation and Universities(Project CTQ2017-83823R)A.Ballesteros-Gomez acknowledges the funding from Spanish Ministry of Science,Innovation and Universities for a Ramon y Cajal contract(RYC-2015-18482).
文摘Bisphenol A(BPA)and replacements,such as bisphenol S(BPS)and F(BPF)and other substitutes(BPSMAE,D-8 and TGSA)have been recently reported in thermal paper.In this study,BPA,BPS,BPF;BPS-MAE,D-8 and TGSA were analysed in indoor dust collected in Spain from different microenvironments(living rooms,bedrooms,cars and offices).Levels were compared with those reported in our previous study(shops and other public microenvironments,where frequent use of thermal paper is expected).We investigated the differences between microenvironments,possible correlations between compounds and estimated the ingestion rates.BPS-MAE,D-8 and TGSA were present at levels up to 529,128 and 356 ng g^-1,respectively,in all the microenvironments.The highest levels were measured in cars.
