Contrast enhancement of vascular walls of intracranial high flow malformations in black blood MRI indicates high inflammatory activity

Background: There are controversies concerning the natural history of arteriovenous malformations (AVMs) in literature and it is not clear which AVMs should be treated and which should be just observed. Objective criteria beyond growth in serial MRIs or angiographies are needed. The use of black blood MRI is currently under investigation for evaluating the rupture risk of cerebral aneurysms, however its use for assessment of AVMs has yet to be evaluated. We therefore conducted a feasibility study on the application of black blood MRI (bbMRI) in AVMs to assess rupture risk. Methods: Retrospective study of 10 patients with intracranial AVMs and 4 patients with arteriovenous fistulas who received a black blood MRI before treatment. Results: AVM niduses (9/10) show contrast enhancement irrespective of rupture or size. All arteriovenous fistulas (4/4) were contrast enhancing irrespective of rupture. Conclusion: High flow malformations are in a permanent stage of inflammation which does not seem to allow conclusions on their rupture risk at the current stage. BbMRI is a feasible method of identifying inflammation in AVMs and arteriovenous fistulas. However, future prospective studies are needed to evaluate whether bbMRI contrast enhancement correlates with rupture risk.

Cavernous brain malformations and their relation to black blood MRI in respect to vessel wall contrast enhancement

Background: Inflammatory responses are implicated as crucial patho-mechanisms of vascular brain malformations. Inflammation is suggested to be a key contributor to aneurysm rupture; however it is unclear whether inflammation contributes similarly to bleeding of cerebral cavernous malformations (CCMs). Black blood MRI is a sequence which identifies inflammation in blood vessel walls and in the present study is used to detect inflammatory response in CCMs. Methods: Fifteen patients with 17 CCMs treated in our department in 2017 were retrospectively analysed. All patients received black blood MRIs and the results were analysed in correlation with, size and bleeding of CCMs. Results: Size and bleeding status of CCMs did not correlate with contrast enhancement in the CCM wall. One of 3 patients with bleeding displayed contrast enhancement in black blood MRI, whereas the others had non enhancing lesions. Because of the small number of cases a statistical analysis was not performed. Conclusion: In this limited cohort, inflammatory reactions in CCMs could not be detected by black blood MRI suggesting that the level of inflammation is minimal in these lesions and those different patho-mechanisms play a more important role in the rupture of CCMs.

Determination of Blood Indices of Guide Black-Fur Sheep in Qinghai Province of China

[ Objective] The research was aimed to provide the basis for the development and utilization of Guide black-fur sheep and its disease di- agnosis and prevention. [ Methed] Blood samples were collected from 15 healthy adult Guide black-fur sheep in Black-Fur Sheep Farm o＇f Guinan County in Qinghai Province. Eighteen blood indices belonging to three classes, erythrocyte, leukocyte and platelet, were determined. E Resultl The blood indices of the Guide black-fur sheep were as follows： the white blood cell count, （7.52 ±2.72） × 109/L; the red blood cell count, （13.04 ± 2.81 ） × 1012/L; the mean corpuscular hemoglobin, （85.36 ＋27.05） g/L; the mean corpuscular volume, （37.22 ± 1.93） fL; the mean corpuscular hemoglobin concentration, （13.19 ±0.96） pg; the platelet count, （370.67 ± 139.60） × 109/L; the lymphocyte absolute count, （14.11 ＋9.36） x 109/L; the absolute count of intermediate cell, （0.79 ±0.46）× 109/L; and the absolute neutrophil count, （0.81 ＋0.71 ） ×109/L.- Conclusion] The reseamh lays the foundation for further Drotectina the aenetic resources of the Guide black-fur sheep and imDrovinQ their production performance.

Blood Typing of Asiatic Black Bear (Ursus thibetanus)

[Objective]The paper was to analyze whether Asiatic black bear( Ursus thibetanus) had different blood group systems. [Method]Whole blood samples were collected from 40 Asiatic black bears in Fujian Province,China. Tube method was used to detect antibodies in plasma,and antibody isotype was determined with 2-mercaptoethanol. Plasma was further analyzed by mass spectrometry. [Result] The plasma from four black bears had antibodies,possibly Ig M isoform,which could agglutinate RBCs from 30 bears. Blood samples from 10 bears were tested by human blood typing reagents. The results showed that four black bears had blood type like human type O,while six bears had like human type B. Mass spectrometry results demonstrated that plasma protein had extensive homology to serum albumin-like isoform 1 found in giant panda( Ailuropoda melanoleuca). [Conclusion]Asiatic black bear might have at least one blood group system with two blood types. If the sick bear needs blood transfusion,a cross-matching test is necessary. Moreover,giant panda might receive blood from Asiatic black bear in case of emergency.

Analyzing of "Xu Sanguan a blood":The Black Humor Artistic

"Black Humor",as a literary genre."Xu Sanguan a Blood" is a novel created by a well-known writer Yu Hua in 1995.It expresses the human desire to survive when the bad luck.The story tells us that Xu Sanguan spent relying on selling blood they had to get a life and overcome adversity.And when he was old,Xu Sanguan knows that nobody should want his blood,his spirit has collapsed.Black humor from this man gives us more heavy consider and let's know what the truth of the life is.Xu Sanguan had the both of many hates and also many hopes about the family and the life in this story.And his black humor makes him crying with the smile and smiling with the tears.

Relationship between Obesity, Serum Uric Acid, Serum Potassium and Glomerular Filtration Rate with Electric Left Ventricular Hypertrophy in Blacks Central Africans with High Blood Pressure

The authors conducted a retrospective study over a period of 6 months in a hypertensive population in order to determine the correlation between serum uric acid on glomerular filtration rate (GFR) and estimated serum potassium with left ventricular hypertrophy (LVH) and obesity. This study enrolled 122 patients including 63 women (51.6%). The mean age was 55.9 ± 10.6 years (range 30 to 74 years). Obesity weight was found in 38 cases (31.1%) of which 20?were men (33.9%) and 18 women (28.6%). Abdominal obesity was found in 104 cases (85.2%). The average serum uric acid in patients with obesity weight was 63.3 ± 18.9 mg/L vs 62.4 ± 14.2 mg/L for no-obese patients (p?= 0.63). The average serum potassium in obese patients was 4.06 ± 0.42 mEq/L vs 4.02 ± 0.46 mEq/L for no-obese (p?= 0.65). The average GFR was 73.4 ± 21.4 ml/L in obese patients vs 66.6 ± 22.6 ml/min in no-obese (p?= 0.03). The LVH was found in 81 cases (66.4%). The LVH was found in 65 (62.5%) obese patients vs?16 (88%) non-obese patients (OR = 4.8, 95% 1.04?-?22?p?= 0.02). Only abdominal?obesity has been correlated with left ventricular hypertrophy after multivariate analysis. Emphasis must be focused on public health actions for effective and appropriate measures against obesity and hypertension, whose prevalence is increasing in our region.

流动敏感黑血序列三维T_(1)WI增强扫描在脑转移瘤中的应用价值

目的:探讨3.0T磁共振流动敏感黑血序列三维T_(1)加权增强扫描(FSBB 3D CE-T_(1)WI)在脑转移瘤中的应用价值。方法:回顾性将2021年1月-2022年8月在本院就诊且经临床和影像检查证实的52例脑转移患者纳入研究。所有患者分别行颅脑FSBB 3D CE-T_(1)WI、FSE CE-T_(1)WI和快速梯度回波序列三维T_(1)加权对比增强(FFE 3D CE-T_(1)WI)扫描,采用卡方检验或Fisher精确概率检验比较3个序列之间病灶检出率的差异。结果:经随访证实颅内转移灶总数为371个,FSBB 3D CE-T_(1)WI的检出率99.73%(370/371),FFE 3D CE-T_(1)WI的检出率为83.02%(308/371),FSE CE-T_(1)WI的检出率为78.44%(291/371),三者之间检出率的差异有统计学意义(χ^(2)=82.748,P<0.05)。分层分析:直径<3 mm的转移灶共160个,FSE CE-T_(1)WI、FFE 3D CE-T_(1)WI和FSBB 3D CE-T_(1)WI的检出率分别为50.62%、60.62%和99.37%,三个序列之间检出率的差异有统计学意义(χ^(2)=101.436,P<0.05);对于直径3~10 mm的转移灶,FSE CE-T_(1)WI、FFE 3D CE-T_(1)WI和FSBB 3D CE-T_(1)WI的检出率分别为99.38%、100%和100%,三个序列之间检出率的差异无统计学意义(P>0.05);对直径>10 mm的转移灶,三个序列的检出率均为100%,差异无统计学意义(P>0.05)。结论:FSBB 3D CE-T_(1)WI在检出脑转移瘤方面要优于常规和高分辨序列MR增强扫描,值得临床推广。

影响氨基黑10B显现指纹因素的系统探究

指纹是犯罪现场的最常见物证之一,更是对犯罪嫌疑人或受害人进行同一认证的重要物证,而由于犯罪现场的复杂性,不同的环境因素会对指纹的显现结果造成影响。以瓷砖上的新鲜血手印这一室内凶案现场常见的指纹物证为研究对象,探究了不同因素对氨基黑10B显现液显现指纹的影响,表明质量分数为0.60%氨基黑10B,质量分数为25%冰乙酸的显现液是显现瓷砖上新鲜血潜手印的最佳配方,同时,该配方也适用于不同显现方法、不同承痕客体、不同浓度和不同遗留时间的血手印和显现含有蛋白质的指纹。

高分辨MRI 3D黑血技术及CT血管成像在急性缺血性脑卒中的应用价值

目的 探究高分辨磁共振成像(MRI)3D黑血技术与CT血管成像(CTA)在急性缺血性脑卒中患者中的应用价值。方法 选取2020-01—2022-12江苏省中西医结合医院的急性缺血性脑卒中患者102例为研究对象,所有患者接受颈部高分辨MRI 3D黑血技术及CTA扫描,比较不同检查方法所得管腔面积、管壁面积、管壁外界面积。以数字减影血管造影(DSA)为金标准,分析高分辨MRI 3D黑血技术及CTA对缺血性脑卒中患者血管狭窄、溃疡斑块的诊断价值。结果 高分辨MRI 3D黑血技术与CTA所测管腔面积、管壁面积、管壁外界面积比较差异无统计学意义(P>0.05)。以DSA为金标准,高分辨MRI 3D黑血技术诊断血管狭窄>70%真阳性62例,假阳性2例,真阴性35例,假阴性3例;CTA诊断真阳性62例,假阳性3例,真阴性34例,假阴性3例。两种方式诊断特异度、敏感度、准确度比较无统计学差异(P>0.05)。高分辨MRI 3D黑血技术诊断溃疡斑块真阳性49例,假阳性1例,真阴性50例,假阴性2例;CTA诊断真阳性38例,假阳性17例,真阴性35例,假阴性12例。高分辨MRI 3D黑血技术诊断溃疡斑块特异度、敏感度、准确度高于CTA(P<0.05)。结论 两种检查方式对急性缺血性脑卒中患者颈动脉狭窄的诊断均有较高参考价值,高分辨MRI 3D黑血技术对于溃疡斑块的诊断参考价值更高。

菌草灵芝多糖肽对闽西南黑兔生长性能、血液生化指标及免疫机能的影响

【目的】研究日粮中添加菌草灵芝多糖肽(JCGLPP)对闽西南黑兔生长性能、血液生化指标及免疫功能的影响,为JCGLPP在肉兔中的应用提供依据。【方法】选取(33±2)d、初始体质量(655.65±25.90)g、健康状况良好的断奶闽西南黑兔公兔100只,随机分为4组,对照组饲喂基础饲粮,试验Ⅰ、Ⅱ、Ⅲ组分别饲喂在基础饲粮中添加50,100和150 mg/kg JCGLPP的日粮,56 d后统计腹泻率和死亡率,测算平均日采食量(ADFI)、平均日增重(ADG)以及料重比(F/G);屠宰后取背腰最长肌,测定pH值、滴水损失率和剪切力;采集血清,测定总蛋白(TP)、白蛋白(ALB)质量浓度,尿素氮(UN)、丙二醛(MDA)浓度,超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性以及IgG、IgA、IgM和IL-6质量浓度;计算心脏、肝脏、肾脏、圆小囊、脾脏、胸腺和蚓突器官指数;测定十二指肠、空肠、回肠黏膜炎性因子IL-1α、IL-2和分泌型免疫球蛋白(sIgA)质量浓度及肠道淀粉酶、纤维素酶和胰蛋白酶活性。【结果】①各组间兔的终末体质量、平均日增重、平均采食量和料重比均无显著差异(P>0.05);对照组兔的腹泻率和死亡率最高,Ⅱ组兔的腹泻率显著低于对照(P<0.05)。②试验组兔肌肉的pH、滴水损失和剪切力均与对照无显著差异(P>0.05)。③Ⅱ组兔血液中的TP、ALB质量浓度及SOD、GSH-Px活性显著高于对照(P<0.05),而UN和MDA浓度显著低于对照(P<0.05)。④Ⅱ组兔血液中的IgG、IgA、IgM质量浓度均显著高于对照(P<0.05),而IL-6质量浓度各组差异不显著(P>0.05)。⑤各组兔免疫器官指数差异均不显著(P>0.05);试验组兔十二指肠、空肠、回肠IL-1α、IL-2和sIgA质量浓度大多显著高于对照(P<0.05),肠道淀粉酶活性均显著低于对照(P<0.05),Ⅰ组兔的肠道纤维素酶和胰蛋白酶活性、Ⅱ组兔的肠道胰蛋白酶活性显著高于对照(P<0.05)。【结论】JCGLPP对闽西南黑兔的促生长作用不明显,但能提高机体的抗氧化和免疫能力,降低腹泻率和死亡率,添加量以100 mg/kg为宜。

Detection and Analysis of Routine Blood Indexes in Guike Pig

[Objectives] In this study,routine blood indexes of Guike pig were detected to clarify quantity variation and morphology distribution of different functional cells（ red blood cells,white blood cells and platelets）,thus providing reference data for immunological evaluation and disease resistance research of Guike pig. [Methods] Blood samples were collected from forty-four 49-day-old healthy Guike pigs（ 22 males and 22 females） once a week for seven consecutive weeks to detect routine blood indexes,and the results were compared with routine blood indexes of Landrace pig and Enshi black pig. [Results]Among red blood cell indexes of Guike pig at different day ages,red blood cell count（ RBC） and hemoglobin count（ HGB） both reached the lowest levels（ 6. 85 × 10^12/L and 97. 1 g/L）at 70 days of age,which were significantly lower than the corresponding indexes at 49,56,63,84 and 91 days of age（ P 〈 0. 05,similarly hereinafter）; mean corpuscular hemoglobin（ MCH） and mean corpuscular hemoglobin concentration（ MCHC） demonstrated basically the same variation pattern,which both reached the lowest levels（ 15. 11 pg and 275. 70 g/L） at 70 days of age and reached the highest levels（ 16. 40 pg and 314. 30 g/L） at 91 days of age; hematocrit（ HCT）,mean corpuscular volume（ MCV） and red blood cell distribution width（ RDW） exhibited relatively stable changes with no significant differences among different day ages（ P 〉 0. 05,similarly hereinafter）. Among white blood cell indexes of Guike pig at different day ages,white blood cell count（ WBC） reached the lowest level（ 13. 71 × 10^9/L） at 63 days of age and reached the highest level（ 17. 52 × 10^9/L） at 84 days of age,with significant differences; neutrophil count（ NEUT）reached the lowest level（ 3. 61 × 10^9/L） at 70 days of age,which was significantly lower than that at 49,56 and 77 days of age; lymphocyte count（ LYMPH） exhibited relatively stable changes and was not significantly affected by the increase in day age. Among platelet indexes of Guike pig at different day ages,platelet count（ PLT）,thrombocytocrit（ PCT） and platelet volume distribution width（ PDW） exhibited relatively stable changes among different day ages,which were not significantly affected by the increase in day age. Compared to 35-day-old Landrace pig and 5-month-old Enshi black pig,most routine blood indexes of Guike pig were similar to or ranged between those of Landrace pig and Enshi black pig except HCT,mean platelet volume（ MPV）,PCT and PDW. [Conclusions]Guike pig has a certain uniqueness in routine blood indexes,which has developed a stable immune system at 49-91 days of age.

3D ASL联合3D T1高分辨黑血管壁成像技术对烟雾病导致TIA的诊断应用

目的探讨磁共振三维动脉自旋标记成像(3D Arterial Spin Labeling imaging,3D ASL)联合3D T1高分辨黑血管壁成像(3D T1 Black Blood imaging,3D T1BB)技术对烟雾病(Moyamoya Disease,MMD)导致短暂性脑缺血发作(Transient Ischemic Attack,TIA)的诊断价值。方法临床上疑似诊断烟雾病导致TIA患者172例,分别行磁共振平扫、3D ASL、3D T1BB和DSA检查,采用DSA检查结果作为诊断烟雾病的金标准,比较3D ASL联合3D T1BB与3D ASL、3D T1BB的敏感度、特异度和准确度差异,并分析3D T1BB图像上斑块的特点。结果根据DSA检查结果显示,3D ASL诊断真阳性为105例,真阴性19例,假阳性7例,假阴性41例,3D T1BB诊断真阳性为112例,真阴性21例,假阳性5例,假阴性34例,3D ASL联合3D T1BB诊断真阳性139例,真阴性18例,假阳性8例,假阴性7例,3D ASL联合3D T1BB诊断烟雾病导致TIA的敏感度、特异度和准确度分别为95.21%、69.23%、91.28%,其敏感度和准确度均高于单独应用3D ASL和3D T1BB(P<0.01),3种诊断方法的特异度比较,差异无统计学意义(P>0.05)。3D ASL联合3D T1BB诊断方法曲线下面积为0.822,高于分别两项单独诊断的曲线下面积(3D ASL,AUC=0.725;3D T1BB,AUC=0.787)。3D T1BB图像显示:172例患者中有MCA血管344根,其中发现有稳定斑块82根(23.84%),易损斑块262根(76.16%),斑块特征为斑块呈向心性狭窄,易损斑块增强扫描可见明显强化。结论3D ASL能够反应脑组织血流灌注情况,3D T1BB能够反应斑块的特征并反映斑块的性质,3D ASL联合3D T1BB对烟雾病导致TIA具有较高的诊断价值。

高分辨MRI3D黑血技术和CT血管造影对颈动脉狭窄患者斑块特性诊断价值比较

目的 比较高分辨磁共振成像(MRI)3D黑血技术和计算机断层扫描(CT)血管造影(CTA)对颈动脉狭窄患者斑块特性的诊断价值。方法 选取2020年1月至12月本院收治的68例临床拟诊为颈动脉狭窄伴斑块患者。所有研究对象均进行颈部MRI 3D黑血技术和CTA检查,比较高分辨率MRI 3D黑血与CTA的定量测量结果。以数字减影血管造影(DSA)为金标准,分析两种检测方法对血管狭窄和溃疡斑块的诊断价值。结果 68例患者中单侧病变10例,双侧病变58例,3支因图像质量不佳予以剔除,共纳入123支颈动脉病变。高分辨率MRI 3D黑血测量的管腔面积、管壁面积及管壁外界面积与CTA比较,差异均无统计学意义(P>0.05)。高分辨率MRI 3D黑血、CTA测量管腔狭窄率比较差异无统计学意义(P>0.05),Pearson相关性分析显示高分辨率MRI 3D黑血、CTA与DSA测量管腔狭窄率均呈正相关(P<0.001)。以DSA为金标准,高分辨率MRI 3D黑血检查对血管狭窄>70%的灵敏度为93.88%、特异度为100.00%、约登指数为0.939;CTA的灵敏度为91.83%、特异度为93.24%、约登指数为0.851。高分辨率MRI 3D黑血和CTA分别检出溃疡斑块38个、35个,DSA检出33个,高分辨率MRI 3D黑血诊断溃疡斑块的灵敏度、特异度和约登指数分别为100.00%、91.44%、0.914,CTA诊断溃疡斑块的灵敏度、特异度和约登指数分别为78.79%、90.00%、0.688。结论 高分辨率MRI 3D黑血技术与CTA对颈动脉狭窄均有较高的诊断价值,但高分辨率MRI 3D黑血技术对溃疡斑块的诊断价值更高。

黑加仑籽粉对高脂血症小鼠血脂的影响

目的研究黑加仑籽粉对高脂血症模型小鼠血脂水平的影响。方法雄性昆明种小鼠90只,适应性喂养1 w后,随机抽取15只作为空白对照组给予基础饲料,其余作为建模组用高脂饲料配以高脂饮物喂养。将建模组小鼠随机分为高脂模型组、辛伐他汀阳性对照组(5 mg·kg^(-1)·d^(-1))和黑加仑籽粉低剂量(1.25 g·kg^(-1)·d^(-1))、中剂量(2.5 g·kg^(-1)·d^(-1))、高剂量(5 g·kg^(-1)·d^(-1))组,连续4 w每日灌胃给予各受试物。4 w后,处死动物,分离血清,测定总胆固醇(TC)、甘油三酯(TG)含量;分离肝脏和主动脉,制作石蜡切片,光镜下观察各组小鼠肝脏组织形态变化及基质金属蛋白酶9(MMP-9)在主动脉的表达情况。结果与空白对照组比较,高脂模型组TC、TG明显升高,差异具有统计学意义(P<0.05),表明造模成功;黑加仑籽粉低、中、高剂量组血清TC、TG均较高脂模型组低,差异具有统计学意义(P<0.05),并具有一定的量效关系;黑加仑籽粉低、中、高剂量组可不同程度的缓解小鼠肝细胞肿胀、动脉管壁脂质沉积等病变,以高剂量组作用效果最佳。结论黑加仑籽粉具有降低实验性高脂血症小鼠血脂的功效,并对肝脏及有助于动脉粥样硬化斑块的稳定。

Effects of <i>Nigella sativa</i>L. Seed Extract on Fatigue, Blood Biochemical Parameters and Thyroid Function in Male Mice

Nigella sativa L. (Black seed), is a traditional herbal medicine that has been used for many purposes. The present study was designed to investigate the effects of hydro-alcoholic extract of nigella sativa L. (NS) on performance of Forced Swimming Test (FST), blood biochemical parameters related to fatigue and thyroid functions. Therefore, Blood Urea Nitrogen (BUN), Creatine Kinase (CK), Lactic Dehydrogenase (LDH), and Total Protein (TP), triiodothyronine T3, thyroxin T4 and TSH tests were investigated. Thirty five male adult mice were randomly divided into five groups: three NS-fed groups, one fluoxetine treated group and one control group. Three NS experimental groups received hydro-alcoholic extract of NS at doses of 50, 100 and 200 mg/kg orally for two weeks. Immobility time decreased in all NS groups compared with control group. Administration of NS significantly increased the concentration of T3 and T4 of the treatment groups. On the contrary, the amount of BUN, CK, LDH, TP and TSH decreased. In conclusion, black seed extract at the experimented doses showed anti-depressant, anti-fatigue and hyperthyroid effects.

磁共振黑血血栓成像结合抗Ⅹa因子活性监测诊治孤立性皮质静脉血栓一例

孤立性皮质静脉血栓(ICoVT)的诊断一直充满挑战,根据患者体质量给予低分子肝素的常规抗凝治疗并不能使全部患者获得满意疗效。该文报道1例经磁共振黑血血栓成像诊断为重症ICoVT,并通过监测抗Ⅹa因子活性调整抗凝药物剂量进行治疗的患者,以期为ICoVT的诊断及精准抗凝治疗提供参考。

不同月龄湘东黑山羊血液生化指标含量及其相关性分析

为研究湘东黑山羊不同月龄的血液生化指标含量及其相关性,选取健康、体型相似的2、6、12月龄湘东黑山羊,测定了血液白蛋白(ALB)、总胆固醇(T-CHO)、谷草转氨酶(GOT)、尿素氮(BUN)、血糖(GLU)、碱性磷酸酶(ALP)、总蛋白(T-PRO)、谷丙转氨酶(GPT)、肌酐(CRE)、肌酸磷酸激酶(CPK)、乳酸脱氢酶(LDH)等11项血液生化指标含量。结果显示,公羊各月龄ALP、CPK含量差异不显著(P>0.05),其余9项指标含量差异显著(P<0.05);母羊各月龄CPK含量差异不显著(P>0.05),其余10项指标含量差异显著(P<0.05)。相同月龄公、母羊间,各项生化指标差异不显著(P>0.05)。ALB与月龄呈极显著正相关(P<0.01)。T-CHO与月龄、ALB均呈极显著负相关(P<0.01)。GOT与T-CHO呈极显著负相关(P<0.01)。BUN与月龄、ALB均呈极显著正相关(P<0.01),与T-CHO呈极显著负相关(P<0.01)。GLU与T-CHO呈极显著正相关(P<0.01),与BUN呈显著负相关(P<0.05),与GOT呈极显著负相关(P<0.01)。ALP与T-CHO呈显著正相关(P<0.05),与月龄呈极显著负相关(P<0.01)。T-PRO与月龄、ALB均呈极显著正相关(P<0.01),与GLU呈显著负相关(P<0.05),与T-CHO、ALP均呈极显著负相关(P<0.01)。GPT与GOT呈极显著正相关(P<0.01),与月龄呈显著负相关(P<0.05),与GLU呈极显著负相关(P<0.01)。CRE与ALB呈显著正相关(P<0.05),与月龄、BUN、T-PRO均呈极显著正相关(P<0.01)。CPK与ALP呈显著正相关(P<0.05)。LDH与T-CHO、ALP呈极显著正相关(P<0.01),与CRE呈显著负相关(P<0.05),与月龄、ALB、T-PRO呈极显著负相关(P<0.01)。该研究可为湘东黑山羊的选种选育、疾病诊断、营养状况的评价等提供参考依据。

铬黑T显现潜血手印最优条件探讨

目的以铬黑T为溶质,不同溶液为溶剂制备新型显现液,确定铬黑T溶液显现潜血手印的最佳配方及使用范围。方法通过考察甲醇、二氯甲烷、丙酮、乙酸乙酯、无水乙醇、蒸馏水六种溶剂、不同的显现液浓度、潜血手印遗留时间、承痕体种类以及血液浓度等因素对显现效果的影响,建立最佳显现液体系并与传统染色法进行对比。结果0.020 mol/L的铬黑T-甲醇溶液显现效果最佳,采用浸泡法、滴显法、喷显法均能清晰显现皮革、玻璃片、瓷砖、红色塑料片、农夫山泉红色珠光膜上的潜血手印。与传统染色试剂氨基黑10B对比,铬黑T-甲醇显现液在显现稀释后的血液和陈旧潜血手印时效果更佳。结论0.020 mol/L的铬黑T-甲醇溶液为最佳显现配方,该方法显现效果好、适用范围较广、配合装置使用易于携带且无需后置处理,可供公安现场办案使用,具有较高的应用价值和应用前景。

PM_(2.5)组分长期暴露对2型糖尿病发病的影响研究

目的评估长期暴露于细颗粒物(fine particulate matter,PM_(2.5))五种组分对2型糖尿病发病的影响。方法基于中国健康与养老追踪调查(China Health and Retirement Longitudinal Study,CHARLS)2011年和2015年的数据,纳入3788名中老年人。采用贝叶斯核机器回归(Bayesian kernel machine regression,BKMR)分析黑碳、铵盐、硝酸盐、有机物、硫酸盐对空腹血糖、糖化血红蛋白和2型糖尿病发病风险的单独效应、交互效应和联合效应。使用后验包含概率(posterior inclusion probabilities,PIP)估计组分的重要程度,PIP>0.5被认定为重要变量。结果研究结果表明黑碳对空腹血糖的影响较大(PIP=0.75),并未发现与糖化血红蛋白和2型糖尿病发病风险有关的重要变量。当其余组分位于第25、50和75百分位数(P_(25),P_(50),P_(75))时,黑碳每升高1个四分位数间距(interquartile range,IQR),空腹血糖分别升高0.59(95%CI:0.13,1.05)、0.61(95%CI:0.19,1.03)和0.57(95%CI:0.11,1.03)mmol/L。五种组分之间不存在交互效应。联合效应分析显示,与所有组分浓度都固定在P_(50)相比,当所有组分浓度都处于P_(70)时,空腹血糖值改变最大,提高0.12(95%CI:0.02,0.21)mmol/L。结论本研究提示PM_(2.5)组分长期暴露对糖化血红蛋白和2型糖尿病发病风险无明显影响,仅黑碳单独暴露和PM_(2.5)组分联合暴露可以提高我国中老年人的空腹血糖,要加强对PM_(2.5)中黑碳的防控,为进一步预防2型糖尿病提供依据。

不同血缘、季节、年份和胎次对湘西黑猪妊娠时间的影响

[目的]通过分析不同血缘、季节、年份和胎次对湘西黑猪妊娠时间的影响,为进一步做好湘西黑猪妊娠工作提供参考。[方法]收集2018年5月—2022年4月1938个妊娠时间的生产数据,通过异方差分析探讨不同因素对妊娠时间的影响。[结果](1)湘西黑猪平均妊娠时间为(114.4±1.4)d;(2)不同血缘对母猪的妊娠时间影响极显著,不同血缘公猪之间的妊娠时间差异不显著;(3)夏季配种的妊娠时间显著长于其他季节;(4)不同配种年份之间的妊娠时间差异显著;(5)低胎次(1~6胎)与高胎次(7~9胎)之间妊娠时间差异显著,高、低胎次组内无显著差异。[结论]母猪血缘、季节、年份和胎次均会影响母猪妊娠时间,在组织规模化生产安排时应有所区别。