Peptides from Alcalase-hydrolyzed soybean protein hydrolysate(SPH)may hold the potential as natural antioxidants.In addition,the effect of human gastrointestinal(GI)tract on peptide bioavailability needs to be explore...Peptides from Alcalase-hydrolyzed soybean protein hydrolysate(SPH)may hold the potential as natural antioxidants.In addition,the effect of human gastrointestinal(GI)tract on peptide bioavailability needs to be explored.In this study,the impact of simulated GI digestion and transepithelial transport on various antioxidant properties of SPH were investigated.SPH displayed DPPH radical scavenging(IC50=4.22 mg/m L),ABTS·+radical scavenging(IC50=2.93 mg/m L),reducing power and metal ion-chelating activities(IC50=0.67 mg/m L).Furthermore,SPH significantly(P<0.05)inhibited the generation of intracellular reactive oxygen species(ROS)in Caco-2 cells.After simulated GI digestion,the antioxidant properties of SPH were enhanced,except for a decrease in ABTS·+radical scavenging activity.After transepithelial transport,the permeates maintained partial antioxidant activity and the LC-MS/MS data further identified the absorbed soybean peptides.These results suggest that SPH contains the antioxidant peptides that are potentially bioavailable and can be regarded as a promising source of functional food ingredients.展开更多
Soybeans are known as a promising source of bioactive peptides.However,knowledge on the antioxidant behaviors of soybean protein hydrolysate(SPH)in the human intestinal epithelium is limited.In this study,SPH was prep...Soybeans are known as a promising source of bioactive peptides.However,knowledge on the antioxidant behaviors of soybean protein hydrolysate(SPH)in the human intestinal epithelium is limited.In this study,SPH was prepared with Alcalase and subsequently ultrafiltered into four peptide fractions as SPH-I(<3 kDa),SPH-II(3~5 k Da),SPH-III(5~10 k Da)and SPH-IV(>10 kDa).The antioxidant properties of SPH and membrane fractions were investigated using different chemical assays and their protective effects against oxidative stress were evaluated using H2 O2-stressed human intestinal Caco-2 cells.Results showed that SPH-I exhibited the strongest 2,2-diphenyl-1-picrylhydrazyl(DPPH)radical scavenging activity(IC50=2.56 mg/m L)and reducing capacity while SPH-III had the best metal ion-chelating activity(IC50=0.29 mg/m L).Both SPH and the peptide fractions dose-dependently suppressed intracellular reactive oxygen species(ROS)accumulation induced by H2O2 in Caco-2 cells,but the strongest inhibitory effect was observed for SPH-I.Amino acid(AA)results revealed that SPH-I was rich in hydrophobic and antioxidant AAs,which could contribute to its stronger antioxidant properties.Additionally,SPH-I protected Caco-2 cells from H2O2-induced oxidative stress via inhibiting lipid peroxidation and stimulating antioxidant enzyme activities.These results suggest that SPH-I and constitutive peptides can be beneficial ingredients with antioxidant properties and protective effects against ROS-mediated intestinal injury.展开更多
使用alcalase、protamex、flavourzyme、木瓜蛋白酶、中性蛋白酶和胰蛋白酶共6种蛋白酶水解菜籽蛋白,研究水解物清除DPPH自由基能力、还原能力和抑制亚油酸过氧化活性。结果表明,6种蛋白酶水解菜籽蛋白的水解物都具有抗氧化活性,但水解...使用alcalase、protamex、flavourzyme、木瓜蛋白酶、中性蛋白酶和胰蛋白酶共6种蛋白酶水解菜籽蛋白,研究水解物清除DPPH自由基能力、还原能力和抑制亚油酸过氧化活性。结果表明,6种蛋白酶水解菜籽蛋白的水解物都具有抗氧化活性,但水解物的抗氧化活性与水解所用酶的种类和水解时间有关,胰蛋白酶和flavourzyme水解物显示了较强的清除DPPH自由基和还原能力,6种蛋白酶水解物抑制亚油酸过氧化活性高于VE,但略低于BHT。alcalase水解菜籽蛋白时,水解10~45 m in的水解物清除DPPH自由基和还原能力较强,延长水解时间并不能提高其抗氧化活性。展开更多
The antioxidant and free radical-scavenging activities of black soybean peptides(BSP)fractions(Fra-Ⅰ,Fra-Ⅱ,Fra-Ⅲ)were investigated using reducing power and DPPH·/superoxide/hydroxyl radical-scavenging assay.Th...The antioxidant and free radical-scavenging activities of black soybean peptides(BSP)fractions(Fra-Ⅰ,Fra-Ⅱ,Fra-Ⅲ)were investigated using reducing power and DPPH·/superoxide/hydroxyl radical-scavenging assay.The reducing power activity of Fra-Ⅲwas closer to that of BHT but lower than that of ascorbic acid.Fra-Ⅲshowed the strongest scavenging activity against free radicals.The radical-scavenging effect was in a dose-dependent manner and the IC50 values for DPPH·,superoxide and hydroxyl radicals were found to be 1.873,1.684 and 1.735 mg/mL,respectively.Amino acid analysis showed that Fra-Ⅲhad high hydrophobic amino acids(HAA)content and hydrophobicity.The molecular weight distribution of Fra-Ⅲwas found to vary from 100 to 1,000 Da mainly.The antioxidant activity of Fra-Ⅲis clearly related to the amino acid composition,the content of HAA and the molecular mass.The present study suggests that BSP with low molecular weight are useful nutritional antioxidant and potential functional factor for anti-aging.展开更多
基金the financial support received from National Natural Science Foundation of China(No.31430067 and 31601475)China Postdoctoral Science Foundation funded project(No.2017M610200)Postdoctoral Foundation of Heilongjiang Province(No.LBH-Z17011)
文摘Peptides from Alcalase-hydrolyzed soybean protein hydrolysate(SPH)may hold the potential as natural antioxidants.In addition,the effect of human gastrointestinal(GI)tract on peptide bioavailability needs to be explored.In this study,the impact of simulated GI digestion and transepithelial transport on various antioxidant properties of SPH were investigated.SPH displayed DPPH radical scavenging(IC50=4.22 mg/m L),ABTS·+radical scavenging(IC50=2.93 mg/m L),reducing power and metal ion-chelating activities(IC50=0.67 mg/m L).Furthermore,SPH significantly(P<0.05)inhibited the generation of intracellular reactive oxygen species(ROS)in Caco-2 cells.After simulated GI digestion,the antioxidant properties of SPH were enhanced,except for a decrease in ABTS·+radical scavenging activity.After transepithelial transport,the permeates maintained partial antioxidant activity and the LC-MS/MS data further identified the absorbed soybean peptides.These results suggest that SPH contains the antioxidant peptides that are potentially bioavailable and can be regarded as a promising source of functional food ingredients.
基金financially supported by the National Natural Science Foundation of China(No.31430067,31601475,31571876,and 31671807)the 13th Five-Year Plan(No.2016YFD0401402)
文摘Soybeans are known as a promising source of bioactive peptides.However,knowledge on the antioxidant behaviors of soybean protein hydrolysate(SPH)in the human intestinal epithelium is limited.In this study,SPH was prepared with Alcalase and subsequently ultrafiltered into four peptide fractions as SPH-I(<3 kDa),SPH-II(3~5 k Da),SPH-III(5~10 k Da)and SPH-IV(>10 kDa).The antioxidant properties of SPH and membrane fractions were investigated using different chemical assays and their protective effects against oxidative stress were evaluated using H2 O2-stressed human intestinal Caco-2 cells.Results showed that SPH-I exhibited the strongest 2,2-diphenyl-1-picrylhydrazyl(DPPH)radical scavenging activity(IC50=2.56 mg/m L)and reducing capacity while SPH-III had the best metal ion-chelating activity(IC50=0.29 mg/m L).Both SPH and the peptide fractions dose-dependently suppressed intracellular reactive oxygen species(ROS)accumulation induced by H2O2 in Caco-2 cells,but the strongest inhibitory effect was observed for SPH-I.Amino acid(AA)results revealed that SPH-I was rich in hydrophobic and antioxidant AAs,which could contribute to its stronger antioxidant properties.Additionally,SPH-I protected Caco-2 cells from H2O2-induced oxidative stress via inhibiting lipid peroxidation and stimulating antioxidant enzyme activities.These results suggest that SPH-I and constitutive peptides can be beneficial ingredients with antioxidant properties and protective effects against ROS-mediated intestinal injury.
文摘使用alcalase、protamex、flavourzyme、木瓜蛋白酶、中性蛋白酶和胰蛋白酶共6种蛋白酶水解菜籽蛋白,研究水解物清除DPPH自由基能力、还原能力和抑制亚油酸过氧化活性。结果表明,6种蛋白酶水解菜籽蛋白的水解物都具有抗氧化活性,但水解物的抗氧化活性与水解所用酶的种类和水解时间有关,胰蛋白酶和flavourzyme水解物显示了较强的清除DPPH自由基和还原能力,6种蛋白酶水解物抑制亚油酸过氧化活性高于VE,但略低于BHT。alcalase水解菜籽蛋白时,水解10~45 m in的水解物清除DPPH自由基和还原能力较强,延长水解时间并不能提高其抗氧化活性。
文摘The antioxidant and free radical-scavenging activities of black soybean peptides(BSP)fractions(Fra-Ⅰ,Fra-Ⅱ,Fra-Ⅲ)were investigated using reducing power and DPPH·/superoxide/hydroxyl radical-scavenging assay.The reducing power activity of Fra-Ⅲwas closer to that of BHT but lower than that of ascorbic acid.Fra-Ⅲshowed the strongest scavenging activity against free radicals.The radical-scavenging effect was in a dose-dependent manner and the IC50 values for DPPH·,superoxide and hydroxyl radicals were found to be 1.873,1.684 and 1.735 mg/mL,respectively.Amino acid analysis showed that Fra-Ⅲhad high hydrophobic amino acids(HAA)content and hydrophobicity.The molecular weight distribution of Fra-Ⅲwas found to vary from 100 to 1,000 Da mainly.The antioxidant activity of Fra-Ⅲis clearly related to the amino acid composition,the content of HAA and the molecular mass.The present study suggests that BSP with low molecular weight are useful nutritional antioxidant and potential functional factor for anti-aging.
