Objective:To study whether adenovirus-mediated human β-nerve growth factor (Ad-hNGFβ) gene has any protective effect on blast hearing impairment. Methods:Deafness was induced by blast exposure (172.0 dB) in 30 healt...Objective:To study whether adenovirus-mediated human β-nerve growth factor (Ad-hNGFβ) gene has any protective effect on blast hearing impairment. Methods:Deafness was induced by blast exposure (172.0 dB) in 30 healthy guinea pigs. On day 7 of blast exposure, Ad-hNGFβ was infused into the perilymphatic space of 20 animals as the study group (hNGFβ group), and artificial perilymph fluid (APF) was infused into the perilymphatic space of the other 10 animals as the control group. At weeks 1, 4 and 8 after blast exposure, the animals were sacrificed and the cochleae were removed for immunohistochemical and HE stainings. Results: Expression of Ad-hNGFβ protein was detected in each turn of the cochlea at the 1st week, with almost equal intensity in all turns. At the 4th week, the reactive intensity of the expression of Ad-hNGFβ protein decreased. At the 8th week, no expression was detectable. The results of HE staining showed that the amount of spiral ganglions in hNGFβ group was significantly greater than that of the control group at week 4 (P<0.01). Conclusion: Ad-hNGFβ can be expressed at a high level and for a relatively long period in the blast impaired cochlea, suggesting that Ad-hNGFβ has a protective effect on cochlear spiral ganglion cells after blast exposure and the efficient gene transfer into cochlea had been achieved without toxicity.展开更多
针对地下矿爆破装药中炮孔图像快速精准识别的难题,通过引入无参注意力模块(Simple Attention Module,SimAm)和更换损失函数(Enhanced Intersection Over Union Loss,EIoU)的方式改进YOLOv7目标检测模型,优化了炮孔识别特征网络;同时采...针对地下矿爆破装药中炮孔图像快速精准识别的难题,通过引入无参注意力模块(Simple Attention Module,SimAm)和更换损失函数(Enhanced Intersection Over Union Loss,EIoU)的方式改进YOLOv7目标检测模型,优化了炮孔识别特征网络;同时采用数据增强方式解决了炮孔样本小且分布不均匀的难题,提升了模型的泛化性能。结果表明,改进模型的检测精度、检测速度比YOLOv7基线模型分别提升了2.96个百分点和13.524帧/s,提升了炮孔识别效率,有利于促进矿产资源爆破开采向少人化、无人化方向发展。展开更多
基金Supported by the"Eleventh Five-Year Plan"Medical Science Research Foundation of the PLA(No.06MA157)
文摘Objective:To study whether adenovirus-mediated human β-nerve growth factor (Ad-hNGFβ) gene has any protective effect on blast hearing impairment. Methods:Deafness was induced by blast exposure (172.0 dB) in 30 healthy guinea pigs. On day 7 of blast exposure, Ad-hNGFβ was infused into the perilymphatic space of 20 animals as the study group (hNGFβ group), and artificial perilymph fluid (APF) was infused into the perilymphatic space of the other 10 animals as the control group. At weeks 1, 4 and 8 after blast exposure, the animals were sacrificed and the cochleae were removed for immunohistochemical and HE stainings. Results: Expression of Ad-hNGFβ protein was detected in each turn of the cochlea at the 1st week, with almost equal intensity in all turns. At the 4th week, the reactive intensity of the expression of Ad-hNGFβ protein decreased. At the 8th week, no expression was detectable. The results of HE staining showed that the amount of spiral ganglions in hNGFβ group was significantly greater than that of the control group at week 4 (P<0.01). Conclusion: Ad-hNGFβ can be expressed at a high level and for a relatively long period in the blast impaired cochlea, suggesting that Ad-hNGFβ has a protective effect on cochlear spiral ganglion cells after blast exposure and the efficient gene transfer into cochlea had been achieved without toxicity.
文摘针对地下矿爆破装药中炮孔图像快速精准识别的难题,通过引入无参注意力模块(Simple Attention Module,SimAm)和更换损失函数(Enhanced Intersection Over Union Loss,EIoU)的方式改进YOLOv7目标检测模型,优化了炮孔识别特征网络;同时采用数据增强方式解决了炮孔样本小且分布不均匀的难题,提升了模型的泛化性能。结果表明,改进模型的检测精度、检测速度比YOLOv7基线模型分别提升了2.96个百分点和13.524帧/s,提升了炮孔识别效率,有利于促进矿产资源爆破开采向少人化、无人化方向发展。
