Changes of IL-8 and IL-8 mRNA after blast-fragment combined injury in dogs

Objective:To explorethe characteristicsand the mechanismof the blast-fragmentcombinedinjury.Methods:Afterthedogswereinflictedwithhigh-velocityfragmentinjuryon theirlefthindlimbsafterblastinjury,theIL-8intheplasmaandlungtissuesupernatantswereassayedwithELISA,andtheexpressionof IL-8mRNAin lungtissuewas detectedwithin situ hybridization.Results:The levelsof IL-8in plasmaandlungtissueswere increasedafterblast,highvelocityfragmentandblast-fragmentcombinedinjuriesrespectively.IL-8mRNAwere upregulatedafterinjuries.Conclusion:IL-8may playa rolein theoccurrenceanddevelopmentof lunginjury.Detectingtheplasmalevelsof IL-8maybe quitehelpfulto estimatetheinjury.