AIM:To assess the retinal thickness and fundus blood flow density changes in chest pain patients with dyslipidemia using optical coherence tomography angiography(OCTA).METHODS:All subjects with chest pain as the main ...AIM:To assess the retinal thickness and fundus blood flow density changes in chest pain patients with dyslipidemia using optical coherence tomography angiography(OCTA).METHODS:All subjects with chest pain as the main symptom accepted a comprehensive ophthalmological examination.According to the serum lipid levels,the participants were divided into the control group and the dyslipidemia group.The retina thickness and fundus blood flow density were determined using OCTA.RESULTS:The study enrolled 87 left eyes from 87 adults with dyslipidemia and 87 left eyes from age-and sexmatched participants without dyslipidemia.The retina of dyslipidemia subjects was significantly thinner than that of the controls in the inferior(P=0.004 and P=0.014,respectively)and temporal(P=0.015 and P=0.019,respectively)regions,both inner and outer layers.In terms of blood flow density in the macula or optic disk,there was a decreasing trend in the dyslipidemia group compared with the control group,especially in the inferior and temporal regions.CONCLUSION:Dyslipidemia may contribute to the decrease in retinal thickness and fundus blood flow density.Further validation of the association between abnormal lipid metabolism and fundus microcirculation alterations needs to be carried out in chest pain patients.展开更多
Functional morphological alterations of human blood platelets induced by oxidatively modified low density lipoprotein (LDL) were studied with an in vitro model by means of electron microscopy, reflection contrast micr...Functional morphological alterations of human blood platelets induced by oxidatively modified low density lipoprotein (LDL) were studied with an in vitro model by means of electron microscopy, reflection contrast microscopy and quantitative image analysis. The oxidized LDL (50-300 μg/ml) induced the disc-sphere transformation of platelets, the formation of pseudopodia, centralization of granules and degranulation. Platelet plasma membrane was damaged by oxidized LDL leading to a lower electron density of he cytoplasm compared to controls. In an incubation chamber, oxidized LDL-treated platelets sedimented onto the展开更多
This paper is aimed to examine if changes in platelet-derived growth factor(PDGF)expression at different stages of cervical cancer are related to the variation in blood vessel density(BVD)and lymphatic vessel density(...This paper is aimed to examine if changes in platelet-derived growth factor(PDGF)expression at different stages of cervical cancer are related to the variation in blood vessel density(BVD)and lymphatic vessel density(LVD)to evaluate the relationship between PDGF expression and stages and metastasis of cervical cancer.Polymerase chain reaction(PCR)and RT-PCR were used to detect the expression levels of PDGF in 45 cervical cancer tissue samples(the experimental group).The samples were immunohistochemically stained with mono-clonal antibodies D2-40 and CD34,and BVD and LVD were measured.The expressions of PDGF-A,-B,and-D were all higher in the experimental group than in the control group(P<0.05);no significant difference was found in the expression of PDGF-C between the experi-mental group and the control group(P>0.05).PDGF-A and-B expression was positively related with BVD and LVD(P<0.01,R=0.49,0.527,0.327,0.68).The expression levels of PDGF-C and-D were not significantly related with BVD and LVD.At the early stage of cervical cancer,BVD and LVD were significantly higher than in the controls(P<0.01).The BVD and LVD in tissues in the surrounding areas of cervical cancer were significantly higher than in tissues at cancer center,and LVD was related to lymph node metastasis(P<0.001).BVD and LVD were not associated with the differentiation and pathological stages of cervical cancer.The expressions of PDGF-A,-B,and-D in cervical cancer were closely related with the clinical stages of cervical cancer.PDGF-A and-B were intimately associated with the lymph node metastasis and prognosis of cervical cancer.展开更多
Objective:To investigate the correlation of variables between dominant and non-dominant eyes in healthy young people.Methods:300 cases(600 eyes)of healthy young volunteers recruited in our hospital from March 2020 to ...Objective:To investigate the correlation of variables between dominant and non-dominant eyes in healthy young people.Methods:300 cases(600 eyes)of healthy young volunteers recruited in our hospital from March 2020 to February 2022were selected as the research objects.The general data of the volunteers were collected and the dominant eye,diopter,biological parameters and retinal blood flow density were detected.We compared all the test indexes of volunteers with different dominant eyes,and compared the refractive parameters,biological parameters and retinal blood flow density between the dominant and non-dominant eyes of volunteers.Results:Among the 300volunteers,62.67%(188/300)had the right eye as the dominant eye and 37.33%(112/300)had the left eye as the dominant eye.There was no significant difference in gender,BMI and equivalent spherical lens(SE)among the subjects with different dominant eyes(P>0.05).The SE and spherical diopter(S)of dominant eye were higher than that of non-dominant eye(P<0.05),but there was no significant difference between the dominant and non-dominant eye(C)(P>0.05);There was no significant difference in SE and C between dominant and non-dominant eye in volunteers with SE difference≤0.5 D and 0.51-1.25 D(P>0.05),and spherical diopter(S)of dominant eye was higher than that of non-dominant eye(P<0.05);The difference of SE was 1.26-2.00 D,>2.00 D,the SE and spherical diopter(S)of dominant eye were higher than those of non-dominant eye(P<0.05),but there was no significant difference between the dominant and non-dominant eye C(P>0.05).The levels of RA and CA in the dominant eye of 300 volunteers were lower than those in the non-dominant eye.The RA and CA levels of the dominant eye were lower than those of the non-dominant eye in volunteers with RA difference≤0.50 D,0.51-1.25 D,1.26-2.00 D,>2.00 D(P<0.05).There was no significant difference in the levels of axial length(AL),central corneal thickness(CCT),anterior chamber depth(ACD)and lens thickness(LT)between the dominant and non-dominant eyes(P>0.05).The levels of superficial retinal capillary layer(SVC),deep retinal capillary layer(DVC)and mean retinal blood flow density(RET)in dominant eyes were lower than those in non-dominant eyes.The level of AC in non-dominant eyes was higher than that in non-dominant eyes(P<0.05).Conclusion:The dominant eye of healthy young people is mostly the right eye,which has nothing to do with gender,age and BMI.Meanwhile,the biological parameters between the dominant and non-dominant eye were basically the same,the mean retinal blood flow density of the dominant eye was lower than that of the non-dominant eye,but its AC was higher than that of the non-dominant eye.展开更多
基金Supported by the Science and Technology Commission of Shanghai Municipality(No.20Y11910800)。
文摘AIM:To assess the retinal thickness and fundus blood flow density changes in chest pain patients with dyslipidemia using optical coherence tomography angiography(OCTA).METHODS:All subjects with chest pain as the main symptom accepted a comprehensive ophthalmological examination.According to the serum lipid levels,the participants were divided into the control group and the dyslipidemia group.The retina thickness and fundus blood flow density were determined using OCTA.RESULTS:The study enrolled 87 left eyes from 87 adults with dyslipidemia and 87 left eyes from age-and sexmatched participants without dyslipidemia.The retina of dyslipidemia subjects was significantly thinner than that of the controls in the inferior(P=0.004 and P=0.014,respectively)and temporal(P=0.015 and P=0.019,respectively)regions,both inner and outer layers.In terms of blood flow density in the macula or optic disk,there was a decreasing trend in the dyslipidemia group compared with the control group,especially in the inferior and temporal regions.CONCLUSION:Dyslipidemia may contribute to the decrease in retinal thickness and fundus blood flow density.Further validation of the association between abnormal lipid metabolism and fundus microcirculation alterations needs to be carried out in chest pain patients.
文摘Functional morphological alterations of human blood platelets induced by oxidatively modified low density lipoprotein (LDL) were studied with an in vitro model by means of electron microscopy, reflection contrast microscopy and quantitative image analysis. The oxidized LDL (50-300 μg/ml) induced the disc-sphere transformation of platelets, the formation of pseudopodia, centralization of granules and degranulation. Platelet plasma membrane was damaged by oxidized LDL leading to a lower electron density of he cytoplasm compared to controls. In an incubation chamber, oxidized LDL-treated platelets sedimented onto the
文摘This paper is aimed to examine if changes in platelet-derived growth factor(PDGF)expression at different stages of cervical cancer are related to the variation in blood vessel density(BVD)and lymphatic vessel density(LVD)to evaluate the relationship between PDGF expression and stages and metastasis of cervical cancer.Polymerase chain reaction(PCR)and RT-PCR were used to detect the expression levels of PDGF in 45 cervical cancer tissue samples(the experimental group).The samples were immunohistochemically stained with mono-clonal antibodies D2-40 and CD34,and BVD and LVD were measured.The expressions of PDGF-A,-B,and-D were all higher in the experimental group than in the control group(P<0.05);no significant difference was found in the expression of PDGF-C between the experi-mental group and the control group(P>0.05).PDGF-A and-B expression was positively related with BVD and LVD(P<0.01,R=0.49,0.527,0.327,0.68).The expression levels of PDGF-C and-D were not significantly related with BVD and LVD.At the early stage of cervical cancer,BVD and LVD were significantly higher than in the controls(P<0.01).The BVD and LVD in tissues in the surrounding areas of cervical cancer were significantly higher than in tissues at cancer center,and LVD was related to lymph node metastasis(P<0.001).BVD and LVD were not associated with the differentiation and pathological stages of cervical cancer.The expressions of PDGF-A,-B,and-D in cervical cancer were closely related with the clinical stages of cervical cancer.PDGF-A and-B were intimately associated with the lymph node metastasis and prognosis of cervical cancer.
文摘Objective:To investigate the correlation of variables between dominant and non-dominant eyes in healthy young people.Methods:300 cases(600 eyes)of healthy young volunteers recruited in our hospital from March 2020 to February 2022were selected as the research objects.The general data of the volunteers were collected and the dominant eye,diopter,biological parameters and retinal blood flow density were detected.We compared all the test indexes of volunteers with different dominant eyes,and compared the refractive parameters,biological parameters and retinal blood flow density between the dominant and non-dominant eyes of volunteers.Results:Among the 300volunteers,62.67%(188/300)had the right eye as the dominant eye and 37.33%(112/300)had the left eye as the dominant eye.There was no significant difference in gender,BMI and equivalent spherical lens(SE)among the subjects with different dominant eyes(P>0.05).The SE and spherical diopter(S)of dominant eye were higher than that of non-dominant eye(P<0.05),but there was no significant difference between the dominant and non-dominant eye(C)(P>0.05);There was no significant difference in SE and C between dominant and non-dominant eye in volunteers with SE difference≤0.5 D and 0.51-1.25 D(P>0.05),and spherical diopter(S)of dominant eye was higher than that of non-dominant eye(P<0.05);The difference of SE was 1.26-2.00 D,>2.00 D,the SE and spherical diopter(S)of dominant eye were higher than those of non-dominant eye(P<0.05),but there was no significant difference between the dominant and non-dominant eye C(P>0.05).The levels of RA and CA in the dominant eye of 300 volunteers were lower than those in the non-dominant eye.The RA and CA levels of the dominant eye were lower than those of the non-dominant eye in volunteers with RA difference≤0.50 D,0.51-1.25 D,1.26-2.00 D,>2.00 D(P<0.05).There was no significant difference in the levels of axial length(AL),central corneal thickness(CCT),anterior chamber depth(ACD)and lens thickness(LT)between the dominant and non-dominant eyes(P>0.05).The levels of superficial retinal capillary layer(SVC),deep retinal capillary layer(DVC)and mean retinal blood flow density(RET)in dominant eyes were lower than those in non-dominant eyes.The level of AC in non-dominant eyes was higher than that in non-dominant eyes(P<0.05).Conclusion:The dominant eye of healthy young people is mostly the right eye,which has nothing to do with gender,age and BMI.Meanwhile,the biological parameters between the dominant and non-dominant eye were basically the same,the mean retinal blood flow density of the dominant eye was lower than that of the non-dominant eye,but its AC was higher than that of the non-dominant eye.