Epidemiological features and dynamic changes in blood biochemical indices for COVID-19 patients in Hebi

BACKGROUND Millions of people have died of coronavirus disease 2019(COVID-19)due to severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)infection,and retrospective studies of the disease in local regions are necessary.AIM To characterize the epidemiological features and dynamic changes in blood biochemical indices for SARS-Co V-2-infected patients in Hebi,a representative city with a large floating population in North China.METHODS From January 25 to February 10,2020,the clinical data of patients who tested positive for SARS-Co V-2 by quantitative real-time polymerase chain reaction in Hebi city(China)were evaluated at admission,and laboratory data for hematologic parameters,inflammatory indices,coagulation function indices,liver function indices,blood lipid indices,renal function indices,myocardial enzyme activities and five blood biochemical markers of immunity were evaluated at admission,upon hospitalization and before discharge.RESULTS Sixteen confirmed COVID-19 patients developed pneumonia but were cured after adequate treatment.Fever and fatigue were the common symptoms.The most common laboratory abnormalities of patients at admission were leukopenia,eosinopenia,decreased percentage of eosinophils,elevated high sensitivity C-reactive protein and fibrinogen levels,hypoalbuminemia,mildly increased aspartate transferase activity and levels of bilirubin,and increased levels ofβ2-microglobulin.Importantly,aggravated liver dysfunction was detected in most patients,which may be partially attributed to virus infection as well as medicinal treatment.CONCLUSION This study provides several potential diagnostic markers and dynamic biochemical indices of disease progression to better prevent,diagnose and treat COVID-19 infection.

Blood Calcium as a Protective Factor against Traumatic Fracture

Purpose: This study aims to investigate the predictive value of blood calcium in the prognosis of traumatic fracture. Methods: A retrospective experimental design was employed, 112 cases (52 non-fracture and 60 traumatic fracture) were randomly selected. The type of fracture complies with WHO-recommended (2019) diagnostic criteria for osteoporosis combined with fracture. The blood pressure (BP) was measured by OMRON’s HEM-7136 model electronic blood pressure monitor. Blood calcium (Ca2+), and blood phosphorus (P) values were measured using Colorimetric Roche kits on a Roche/Hitachi fully automated biochemical analyzer. Data collection and analysis followed. Results: Higher levels of age, systolic and diastolic blood pressures were found in the traumatic fracture group compared to the control group, whereas weight, height, and blood phosphorus did not differ significantly (P adjusting for age, systolic blood pressure, diastolic blood pressure, and blood phosphorus, binary logistic regression analysis revealed that blood calcium was a protective factor against traumatic fracture (β = -26.85, OR = 0.00, 95% CI = 0.00 -0.02, P = 0.022). Conclusion: The findings suggest that high and low blood calcium levels may serve as useful indicators in predicting the prognosis of fractures resulting from trauma.

Bio-Effect of Rare Earths in RE-High Background Region I. Some Blood Biochemical Indices from Population Resided in Light REE District

The results of biochemical indices in human blood show that there are significant difference in the population mean of these indices between the LREE high region in South Jiangxi, China and the control. In comparison with the normal region, the contents of total serum protein(TSP), albumin(AL), β globulin (β G), glutamic pyruvic transitanase (GPT), serium triglycerides (STG) and immunoglobulin(IgA) are manifested as low, but high cholesterol (CHO) in the LREE high region. These deviations may be related to the REE concentration and composition in food chains, and also are sex dependent. Certain blood indices (such as TSP) of different age groups in the LREE high region indicate that the influence of REE on males is a one way irreversible process while females show a strong ability of restoration.

Effects of Coated Methionine on the Growth Performance, Blood Biochemical Indices and Nutrient Apparent Ileal Digestibility in Broilers

[ Objective] This trial was conducted to study the effects of different coated methionines on the growth performance, blood biochemical indices and apparent ileal digestibility of amino acids in broilers. [ Method ] A total of 3 780 Cobb 500 broilers aged 18 days were randomly arranged into 7 groups with 3 replicates per group and 180 broilers per replicate. Control group （A） received the basal diet supplemented with O. 16% DL-me- thionine, and trial groups received the basal diets supplemented with 0.32% （ B）, 0.29% （ C）, 0.26% （ D）, 0.22% （ E）, 0.19% （F） and 0.16% （G） of coated methionine, and broilers were fed to the age of 42 days. ＂[ Result] The final weight, average daily gain and average daily feed intake of groups B and C were higher than those of the control group （ P〈0.05）, and the mortality of group B was lower than that of the control group （ P 〈0.05）, but there was no significant difference in all above indices between the other groups and the control group （P 〉 0.05）. Plasma GSH-Px activity of trial groups （ except group G） was higher than that of the control group （ P 〈 0.05）, and SOD activity of groups B and C were higher than that of group G （P 〈0.05）, but there were no significant differences in other blood biochemical indices among all groups （P 〉0.05）. There were no significant differences in apparent ileal digestibility of dry matter and crude protein between trial groups and the control group （ P 〉0.05 ）, and the apparent ileal digestibility of amino acids of trial groups was increased （P 〈0.05） or no change expect that the apparent ileal digestibility of many kinds of amino acids of group G was decreased compared with the control group （P〈0.05）. [ Concluslon~ The growth performance and apparent ileal digestibility of amino acids in broilers were increased with the rising supplementation of coated methionine, but the supplementation of 0.19% coated methionine can meet the needs of broilers, namely, coated methionine can be substituted for 60% DL-methionine in the diet, so DL-methio- nine can be saved up to 40%.

Determination of Blood Indices of Guide Black-Fur Sheep in Qinghai Province of China

[ Objective] The research was aimed to provide the basis for the development and utilization of Guide black-fur sheep and its disease di- agnosis and prevention. [ Methed] Blood samples were collected from 15 healthy adult Guide black-fur sheep in Black-Fur Sheep Farm o＇f Guinan County in Qinghai Province. Eighteen blood indices belonging to three classes, erythrocyte, leukocyte and platelet, were determined. E Resultl The blood indices of the Guide black-fur sheep were as follows： the white blood cell count, （7.52 ±2.72） × 109/L; the red blood cell count, （13.04 ± 2.81 ） × 1012/L; the mean corpuscular hemoglobin, （85.36 ＋27.05） g/L; the mean corpuscular volume, （37.22 ± 1.93） fL; the mean corpuscular hemoglobin concentration, （13.19 ±0.96） pg; the platelet count, （370.67 ± 139.60） × 109/L; the lymphocyte absolute count, （14.11 ＋9.36） x 109/L; the absolute count of intermediate cell, （0.79 ±0.46）× 109/L; and the absolute neutrophil count, （0.81 ＋0.71 ） ×109/L.- Conclusion] The reseamh lays the foundation for further Drotectina the aenetic resources of the Guide black-fur sheep and imDrovinQ their production performance.

Effects of Chinese Herbal Compound on the Blood Physiological Indices and Cytokines of Myelosuppressive Mice

[ Objective ] The research aimed to study the effects of Chinese herbal compound on the blcod physiological indices and cytokines of myelosuppressive mice. [ Metho] Myelasuppressive mice model was established by intraperitoneal injection of 80 mg/kg cyclophosphamide. Chinese herbal compound was composed of Houttuynia cordata, Taraxacum mongolicum, Citrus reticulata peel, Atractylodes chinensis, Paeonia sterniana, Atractylodes macrocephala and Angelica sinensis. The effects of Chinese herbal compound and Astragalus polysaccharide at different doses on the blood physiological indices and hematopoietic growth factors of myelosuppressive mice were discussed. [ Result] Myelosuppressive mice medel was successfully established. The total white blood cell count, total nentrephile granulocyte count, total lymphocyte count, total platelet count and the contents of serum interleukin-6 and erythropoietin in myelosuppressive mice were significantly decreased. The total erythrocyte count, the contents of hemoglobin and imerleukin-3 were decreased, without significant difference. The blood physiological indices and the contents of interleukin-3 and erythropoietin in myelosuppressive mice could be improved by intragastric administration of Astragalus polysaccharide and Chinese herbal compound at different doses for 3 days or 7 days. The effect of 20 g/kg Chinese herbal compound was the best after administration for 3 days, and the effect of 10 g/kg Chinese herbal compound was the best after administration for 7 days. The total white blcod cell count, total neutrophile granulocyte count, percentage of neutrephile granulocyte, total lymphocyte count, total count of middle cells, percentage of middle cells, total platelet count, contents of interleukin-3 and erythropoietin in myelosuppressive mice could be extremely significantly improved by intragastric administration of 10 g/kg Chinese herbal compound for 7 days（ P 〈 0. 01 ） , and the reduction of red blood cell count, hematecfit, the contents of hemoglobin and interleukin-6 induced by cyclophesphamide could be inhibited （P 〈 0.01 ）. [ Conclusion ] Chinese herbal compound could improve the hematopoietic function of myclosuppressivc mice induced by cyclophosphamide and its effect was better than Astragalus polysaccharide. 20 g/kg Chinese herbal compound reacted fast and the reaction of 10 g/kg Chinese herbal compound was slow ,but its efficacy was lasting.

Study on The Blood Physiological Indices of Wild Yak, Domestic Yak and Their Crossbreeds

Some blood physiological indices were determined in wild yak,Datong yak(the crossbreeds of the wild and domestic yak)and Tianzhu Black yak in a year. The red blood cell amount(RBC)and packed cell volume (PCV)of the wild yak and Datong yak were similar but differed largely from that of the Tianzhu Black yak. RBC of the wild yak and Datong yak were larger than that of the Tianzhu Black yak and their RBC varied seasonally.The results indicated that wild yak and Datong yak could effectively adapt to changes in environment by adjusting their physiological indices.

Comparative Analysis of Budesonide Treatments on Blood Gas and Inflammation in Chronic Obstructive Pulmonary Disease Remission

Objective:To investigate the effects of budesonide on blood gas and inflammation indexes in patients with chronic obstructive pulmonary disease(COPD)during remission.Methods:Fifty-one patients with COPD in remission,admitted to Zhongshan Hospital of Dalian University from July 2021 to December 2022,were selected and divided into two groups based on a randomized numerical table method.The control group(25 cases)received budesonide formoterol treatment,while the observation group(26 cases)received budesonide geforce treatment.Various indexes,including clinical efficacy,blood gas indexes,inflammation indexes,St.George’s Respiratory Questionnaire(SGRQ)scores,Chronic Obstructive Pulmonary Disease Assessment Test(CAT)scores,and 6-minute Walking Distance Test(6MWD)results,were compared between the two groups.Results:After 21 days of treatment,the total clinical effectiveness rate of the observation group was higher than that of the control group,with a statistically significant difference(P<0.05).Post-treatment,the PaO2 level and pH value in both groups were higher,and the PaCO_(2) level was lower compared to pre-treatment levels.The observation group showed better improvements in these indicators than the control group,with statistically significant differences(P<0.05).SGRQ and CAT scores for both groups were lower post-treatment,with the observation group scoring lower than the control group.Additionally,the 6MWD results were farther for both groups post-treatment,with the observation group achieving greater distances than the control group,with statistically significant differences(P<0.05).Conclusion:Budesonide can effectively improve blood gas indexes in patients with COPD in remission,alleviate related clinical symptoms,reduce inflammatory responses,and promote patient recovery.The treatment efficacy is significant.

Effects of Atrazine on Blood Indices and Histopathology of Grass Carp (Ctenopharhyngoden idella)

Atrazine is currently the most widely used herbicide in the south of Iran. The chronic toxicity effects of atrazine(herbicide) on blood indices and histopathology of the gill, liver and kidney in grass carp(Ctenopharhyngoden idella) were studied by exposing them to sublethal concentrations of atrazine to 0, 10, 100, 1 000, 10 000 g atrazine per liter of water for a period of 30 days. Up to 100 g·L-1, atrazine did not show significant toxicity.In contrast, atrazine in the concentration of 1 000 and 10 000 g·L-1significantly increased toxicity effects on studied parameters. The highest mortality rate(100%) was observed at 10 000 g·L-1on the day 10 and at 1 000 g·L-1on the day 20. Cytological analysis showed that haematocrit(Hct) increased significantly with the increase of atrazine concentration and days. White blood cells(WBC), red blood cells(RBC) and hemoglobin(Hb) were changed significantly(p < 0.05) in different treatments. Biochemical analysis indicated that plasma total protein, albumin, glucose, cholesterol and triglycerides levels decreased significantly(p < 0.05) with the increase of atrazine concentrations and exposure days. Histopathological studies showed hyperplasia, hyperemia, necrosis, mucosa secretion, shortness of filaments, lamellar fusion in gills; necrosis, anemia, bile stagnation in liver; and cell atrophy, hyperemia, hemorrhage and necrosis in kidney which were more severed at 1 000 and 10 000 g·L-1. Therefore, mentioned effects on studied histopathological and some blood parameters were both dose-and time-dependent. In sum, the atrazine exposure of grass carp at concentrations 1 000 and 10 000 g·L-1induced significant changes in blood indices and structural disruptions in gill, liver and kidney organs on days 20 and 10, respectively. The obtained results indicated the atrazine is toxic and could damage vital organs and blood of grass carp. Therefore, the use of atrazine should be imperative and carefully monitored.

The influences of SE infection on layers' production performance,egg quality and blood biochemical indicators

Background： Salmonella enterica serovar Enteritidis （SE）, as a major cause of foodborn illness, infects humans mainly through the egg. However, the symptom of laying hens usually is not typical and hard to diagnosis. In the present study, it is studied that the influences of SE infection on layers＇ performance, egg quality and blood biochemical indicators. It will help us to improve the strategy to control SE infection in commercial layers. One hundred layers at 20 wk of age were divided into 2 groups, 60 hens for experiment and others for control. The experiment group was fed with the dosage of 108 CFU SE per hen. The specific PCR was used to detect the deposition of SE. On the 8 d after SE infection, 10 hens from the control group and 30 hens from the experimenta group were slaughtered to detect the SE colonization. The production performance, egg quality and blood biochemical indices were also analyzed. Results： The results showed that the colonization rate of SE was highest in caecum contents （55.17%） and lowest in vagina （17.24%）. For the eggs the detection rate of SE was highest on the eggshell （80.00%） and lowest in yolk （18.81%）. SE infection had no significant influence on production performance and egg qualities （P 〉 0.05）. The difference of laying rate between the experimental and control groups was less than 0.30%, and both were approximately equal to 82.00%. The blood analysis showed that the aspartic aminotransferase （AST） and alanine aminotransferase （ALT） of experimental group was significantly higher than those of control group （P 〈 0.05）. For experimental and control groups AST values were 236.22 U/I and 211.84 U/I respectively, and ALT values were 32.19 U/I and 24.55 U/I. All of coefficients were less than 20%. The colonization of SE in organs increases the enzyme activities of AST and ALT in blood. Conclusions： SE in feed could invade the oviduct and infect the forming eggs. It significantly increased the concentration of ALT and AST in blood. However,SE infection was hard to be observed from the appearances of layer and egg. It might be a dangerous risk to human health.

Food Consumption Pattern, Anthropometric Indices and Health Status of Adult Women Aged 30-60 Years in Amucha Autonomous Community, Njaba L.G.A., Imo State

The study assessed the anthropometric indices and health status of adult women,aged 30-60 years in relation to their food consumption pattern.The blood sugar level,blood pressure and body mass index of the respondents were determined.A cross sectional survey was used for this study,a clustered and simple random sampling selection technique was used in selecting the samples.Anthropometric indices of weight and height were used to assess the body mass index(BMI),while waist hip ratio(WHR)was used to check for abdominal obesity.Women were considered overweight using world health organization standard(BMI at or above 25),or WHR above 0.85.Health status was assessed by checking blood pressure and blood sugar levels.Blood pressure was taken twice using Omron sphygmomanometer with appropriate cuff off size and women were classified hypertensive if having a mean blood pressure above 120/80 mmHg.Evolve glucometer was used to check for random blood sugar(RBS)levels,respondents were considered diabetics if they have a random sugar above 140 mg/dL.Food frequency table was used to assess food consumption pattern.Respondents were said to have low consumption if consumed the food 1-3 times per week or if rarely or never consumed.It is moderate if consumed 4-6 times per week and high,if consumed 7 times per week,or daily.Questionnaire covered relevant data such as personal data,demographic data.Result showed that the women mean standard deviations for body mass index(BMI),waist hip ratio(WHR),random blood sugar(RBS),systolic blood pressure(SBP)and diastolic blood pressure(DBP)were 26.29±4.1,0.86±0.34,112.51±23.0,115.4±23.0 and 88.45±4.3 respectively.The mean value for BMI was on the overweight region.Bivariate correlation analysis showed that SBP,DBP,RBS and WHR had positive correlation with BMI with confidence level set at 0.05%(0.206,p=0.005;0.16,p=0.031;0.081,p=0.0021 and 0.148,p=0.000 respectively).WHR also had stronger correlation with RBS(0.90,p=0.278),systolic(0.272,p=0.002),diastolic(0.21,p=0.045).This confirms the assertion that WHR should be used as an indicator of cardiovascular disease not BMI.The survey also reveals that 3.6%were underweight.49.0%normal,29.9%overweight and 17.5%obese.Blood pressure levels were marginal as 30.04%,35.4%,34.3%are normal,pre hypertensive and hypertensive respectively.Most of the respondents had normal blood sugar as 91.2%were normal.Overweight,hypertension and diabetes were prevalent in rural communities as observed in this study.There is a need for routine check of WHR among women in clinical practice and epidemiological surveys,to reduce incidence of cardiovascular diseases.There is a need for promotion of a healthy life style,regular exercise and proper nutrition.

What,why and how to monitor blood glucose in critically ill patients

Critically ill patients are prone to high glycemic variations irrespective of their diabetes status.This mandates frequent blood glucose(BG)monitoring and regulation of insulin therapy.Even though the most commonly employed capillary BG monitoring is convenient and rapid,it is inaccurate and prone to high bias,overestimating BG levels in critically ill patients.The targets for BG levels have also varied in the past few years ranging from tight glucose control to a more liberal approach.Each of these has its own fallacies,while tight control increases risk of hypoglycemia,liberal BG targets make the patients prone to hyperglycemia.Moreover,the recent evidence suggests that BG indices,such as glycemic variability and time in target range,may also affect patient outcomes.In this review,we highlight the nuances associated with BG monitoring,including the various indices required to be monitored,BG targets and recent advances in BG monitoring in critically ill patients.

基于超声诊断对中老年食蟹猴心脏结构和功能的基础研究

目的 针对10岁以上中老年食蟹猴开展心脏结构和功能基础研究,为研究老年疾病动物选择提供基础数据。方法 本研究选取144只10岁以上中老年食蟹猴作为研究对象,分为10~15岁组雌性37只、雄性66只,16~20岁组雌性21只、雄性20只,通过一般指标(身体质量指数、血压、心率)、血液生化指标(血糖、血脂和离子指标)、心脏结构和功能指标比较分析,获得中老年食蟹猴心脏结构和功能相关基础数据。结果 10~15岁组和16~20岁组,一般指标比较,随着年龄增加,雌猴和雄猴血压和心率升高,雄猴血压变化具有显著性差异。两组血液生化指标比较,随着年龄增加,雌猴和雄猴血糖、甘油三酯、总胆固醇、低密度脂蛋白胆固醇、血钙、血钠、血钾都显示升高,其中,血糖、甘油三酯(雄)、总胆固醇、高密度脂蛋白胆固醇(雄)、低密度脂蛋白胆固醇(雄)、血钙、血钠、血钾变化具有显著性差异;乳酸脱氢酶显示下降,具有显著性差异。两组心脏收缩功能比较,随着年龄增加,雌猴和雄猴左房前后内径都下降,具有显著性差异;雌猴室间隔收缩末径,左心室舒张和收缩末径显示减小,左心室舒张末容积,左心室收缩末容积,左心室质量指数下降,具有显著性差异,雄猴变化不显著。两组心脏舒张功能比较,随着年龄增加,雄猴二尖瓣舒张晚期速度下降,具有显著性差异;雌猴左室侧壁舒张早期速度升高,具有显著性差异。对雌猴和雄猴代谢指标与心脏结构和功能指标进行相关性分析,结果雌猴代谢指标与心脏结构和功能指标的相关性较弱,Γ绝对值最高不超过0.39,雄猴代谢指标与心脏结构和功能指标的相关性相对较强,Γ值绝对值最高达到0.66。结论 基于超声诊断结合代谢指标对食蟹猴心脏功能进行研究,建立中老年食蟹猴心脏结构和功能相关指标基础数据,随着年龄的增加,食蟹猴的血糖、血脂指标升高,心脏收缩和舒张功能有下降趋势,与中老年人群变化相似,为开展心脏功能相关的老年性疾病研究动物选择提供数据支撑。

利拉鲁肽治疗冠心病合并2型糖尿病患者的临床研究

目的 分析利拉鲁肽治疗冠心病合并2型糖尿病患者的临床效果。方法 102例冠心病合并2型糖尿病患者,应用电脑随机选择方式将患者分为对照组和观察组,每组51例。对照组给予二甲双胍治疗,观察组给予二甲双胍结合利拉鲁肽治疗。对比两组心功能指标(左室射血分数、左室舒张末内径、心排血量以及QT离散度)、血糖指标[空腹血糖(FPG)、餐后2 h血糖(2 h PG)、糖化血红蛋白(HbA1c)]、胰岛素抵抗情况[胰岛β细胞功能指数(HOMA-β)、胰岛素抵抗指数(HOMA-IR)]。结果 治疗后,观察组左室射血分数(56.10±9.06)%、左室舒张末内径(45.80±6.64)mm、心排血量(5.60±1.18)L/min、QT离散度(45.03±6.31)ms优于对照组的(50.50±7.90)%、(50.20±7.07)mm、(4.88±1.01)L/min、(53.77±8.50)ms(P<0.05)。治疗后,观察组2 h PG(7.28±1.30)mmol/L、FPG(6.06±0.27)mmol/L、HbA1c(5.88±0.32)%优于对照组的(8.71±1.20)mmol/L、(7.01±0.76)mmol/L、(6.65±0.52)%(P<0.05)。治疗后,观察组HOMA-β、HOMA-IR优于对照组(P<0.05)。结论 结合冠心病合并2型糖尿病患者实际情况应用二甲双胍结合利拉鲁肽治疗,能够有效改善患者的心功能指标、血糖指标及胰岛素抵抗情况,具有优良的应用前景,在今后的工作过程中能够进一步应用。

青少年刷牙频率与血液指标的相关性:横断面观察性研究

目的分析青少年刷牙频率对血液指标的相关性,初步探讨刷牙频率对青少年全身健康的影响。方法采用便利抽样法,纳入济宁市第七中学2021级学生和济宁市体育训练中心体育训练学生为研究对象。采用单因素和多因素线性回归分析青少年刷牙频率对嗜酸性粒细胞百分比(eosinophil percentage,EOS%)、嗜酸性粒细胞计数(eosinophil count,EOS#)、嗜碱性粒细胞百分比(basophil percentage,BAS%)、嗜碱性粒细胞计数(basophil count,BAS#)、过氧化物歧化酶(superoxide dismutase,SOD)、胰岛素样生长因子1(insulin-like growth factor 1,IGF-1)、尿酸(uric acid,UA)等血液指标的影响。结果共纳入305名青少年(济宁市第七中学167名、济宁市体育训练中心138名),其中男性192名(62.95%),女性113名(37.05%)。根据每日刷牙频率分为3组,分别为≤1次组(40.33%,123/305)、2次组(53.77%,164/305)和≥3次组(5.90%,18/305)。单因素线性回归分析显示,每日刷牙2次与EOS%、EOS#、BAS%和BAS#均呈显著负相关(β均<0,P均<0.05),与SOD和IGF-1均呈显著正相关(β均>0,P=0.028,P=0.007);每日刷牙≥3次与UA呈显著负相关[β(95%CI):-0.16(-0.27~0.04),P=0.008]。多因素线性回归分析显示,每日刷牙2次与BAS%呈显著负相关(β<0,P=0.042)、与SOD和IGF-1均呈显著正相关(β均>0,P=0.036,P=0.012);每日刷牙≥3次与UA呈显著负相关(β<0,P=0.026)。亚组分析结果表明,男性青少年每日刷牙2次与IGF-1呈显著正相关(β>0,P=0.044),女性青少年每日刷牙≥3次与UA呈显著负相关(β<0,P=0.014)。结论青少年刷牙频率与多项影响全身健康的血液指标具有相关性,提示口腔健康与全身健康可能存在潜在关联。

低蛋白质氨基酸平衡饲粮添加谷氨酸对断奶仔猪生长性能、血液抗氧化和免疫指标的影响

试验旨在研究较大幅度降低饲粮粗蛋白质(CP)水平,平衡必需氨基酸并添加谷氨酸对断奶仔猪生长性能、血液抗氧化和免疫指标的影响。试验选用144头杜长大杂交仔猪,随机分成3组,每组6个重复,每个重复8头猪,试验期16 d。Ⅰ组(对照组)饲粮CP水平为21.16%,Ⅱ组饲粮CP水平为15.97%,补充必需氨基酸(EAA)赖氨酸(Lys)、蛋氨酸(Met)、苏氨酸(Thr)、色氨酸(Trp)、缬氨酸(Val)和异亮氨酸(Ile),Ⅲ组为Ⅱ组+谷氨酸(Glu)。结果表明,仔猪平均日增重(ADG)Ⅱ组显著低于Ⅰ组和Ⅲ组(P<0.05)。Ⅱ组仔猪腹泻率显著低于Ⅰ组(P<0.05),Ⅲ组显著低于Ⅱ组(P<0.05),Ⅲ组极显著低于Ⅰ组(P<0.01)。Ⅲ组血浆总抗氧化能力(T-AOC)显著高于Ⅰ组和Ⅱ组(P<0.05)。血浆免疫球蛋白G(IgG)、免疫球蛋白A(IgA)、免疫球蛋白M(IgM)和白细胞介素-10(IL-10)含量Ⅲ组显著高于Ⅰ组和Ⅱ组(P<0.05),Ⅱ组血浆IL-10含量显著低于Ⅰ组(P<0.05)。综上,断奶后饲喂低蛋白质氨基酸平衡并添加谷氨酸的饲粮能够提高断奶仔猪生长性能、减少腹泻、增强机体抗氧化和免疫功能。

化瘀丸治疗中晚期恶性肿瘤患者血液高凝状态的临床研究

目的:探讨化瘀丸治疗中晚期恶性肿瘤患者血液高凝状态的临床疗效和安全性。方法:选取2018年6月至2021年12月北京中医医院肿瘤科门诊和病房的中晚期恶性肿瘤血液高凝状态患者72例作为研究对象。采用前瞻性队列研究,根据是否应用化瘀丸分为观察组(n=35)和对照组(n=37)。对照组接受必要的物理对症支持治疗,观察组在对照组基础上口服化瘀丸8周,2组共随访24周。观察2组患者的血液高凝状态改善率、凝血指标变化、中医证候积分、静脉血栓栓塞(VTE)大出血发生,以及不良事件发生情况。结果:4周后,观察组血液高凝状态改善率为43.75%,对照组为29.41%(P>0.05);8周后,观察组血液高凝状态改善率为65.63%,对照组为41.18%(P<0.05)。进一步分析凝血相关指标变化,发现4周和8周后观察组血浆纤维蛋白原(FIB)低于对照组(P<0.05)。观察组患者中医证候积分改善的总有效率为50.00%,对照组为14.81%(P<0.05),2组患者均未出现大出血情况,观察组未发生药物相关不良事件。结论:化瘀丸可以在必要的物理支持治疗外,进一步改善中晚期恶性肿瘤患者血液高凝状态,缓解血瘀证相关症状。

血常规指标与早产儿视网膜病变发病率及严重程度的相关性

目的分析血常规指标与早产儿视网膜病变发病率(retinopathy of prematurity,Rop)及严重程度的相关性,为临床防治及减轻ROP严重程度提供依据。方法回顾性系列病例研究(Retrospective case study),选取自2018年9月至2023年9月到昆明市儿童医院进行眼底检查的早产儿648例,男371例,女277例,分为无ROP组和ROP组包括阈值前ROP、阈值ROP及急进型后极部ROP(A-ROP),收集患儿胎龄、出生体重、血常规各参数,采用卡方检验检测性别组间差异,单因素方差分析胎龄、出生体重的组间差异,采用秩和检验检测血常规各项数据组间差异,及Logistic回归分析确立相关独立危险因素,并通过绘制受试者工作特征(ROC)曲线,评估LMR及NLR对ROP的早期预测价值。结果性别在组间比较差异无统计学意义(P>0.05),BW、GA在4组间比较中差异具有统计学意义(P<0.05)。3组ROP相较于无ROP组患儿WBC、N、M、HB、NLR比值均明显降低,LMR比值明显增高,差异均有统计学意义(P<0.05)。多因素Logistic回归分析提示BW、GA、HB、NLR、N、M、LMR是发生ROP的独立危险因素(P<0.05)。ROC曲线显示,LMR的曲线下面积为0.651,LMR对ROP具有一定早期预测价值。结论BW、GA、HB、NLR、N、M、LMR是ROP的独立危险因素,LMR对ROP具有一定早期预测价值。

大量输血后患者相关生化指标的变化及临床价值

目的讨论大量输血对患者各项生化指标的影响。方法选择需接受输血治疗的患者100例,根据输血量不同分为对照组(输血量≤2000 ml)与观察组(输血量>2000 ml),各50例。观察患者输血前后凝血功能指标[血浆凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)、纤维蛋白原(FIB)]、血气分析指标(动脉血pH、血乳酸、碱剩余)、血清电解质水平[钠离子(Na^(+))、钾离子(K^(+))、氯离子(Cl^(-))、钙离子(Ca^(2+))]、生化指标[直接胆红素(DBil)、总胆红素(TBil)、乳酸脱氢酶(LDH)、丙谷转氨酶(ALT)、谷草转氨酶(AST)]水平。结果观察组输血后PT、APTT、TT、FIB分别为(24.12±6.63)s、(24.94±6.81)s、(27.01±5.42)s、(1.63±0.61)g/L,对照组输血后PT、APTT、TT、FIB分别为(16.80±3.31)s、(37.41±13.89)s、(17.11±2.01)s、(2.76±1.38)g/L;观察组患者输血后PT、TT长于本组输血前及对照组输血后,APTT短于本组输血前及对照组输血后,FIB低于本组输血前及对照组输血后,存在明显差异(P<0.05)。观察组输血后动脉血pH、血乳酸低于本组输血前及对照组输血后,碱剩余高于本组输血前及对照组输血后,存在明显差异(P<0.05)。观察组输血后Na^(+)、K^(+)、Cl^(-)水平均高于2^(+)本组输血前及对照组输血后,Ca显著低于本组输血前及对照组输血后,存在明显差异(P<0.05)。观察组输血后DBil、TBil、LDH、ALT、AST均显著高于本组输血前及对照组输血后,存在明显差异(P<0.05)。对照组输血前后PT、APTT、TT、FIB、动脉血pH、血乳酸、碱剩余、Na^(+)、K^(+)、Cl^(-)、Ca^(2+)、DBil、TBil、LDH、ALT、AST比较,未见显著差异(P>0.05)。结论大量输血尽管对挽救患者生命及促进后续治疗具有积极作用,但将导致患者凝血功能、血气分析、生化指标及血清电解质水平发生明显变化,输血前后均应做好患者各项生化指标的监测与比较工作,在保障治疗效果的基础上进一步提升临床输血的安全性。