Study on Polymorphisms in the Blood Protein of Tibetan Mastiff

[ Objective] The aim was to study the protein polymorphism in the blood of Tibetan Mastiff, and provide some theoretical basis for resource protection and reasonable development and utilization of Tibetan Mastiff varieties. [ Method] A total of 103 blood samples were taken from four populations of Hequ Tibetan Mastiff, Qinhai Tibetan Mastiff, Tibetan Spaniel and native dogs of Qinghai. Seven blood protein Iocus（Tf, Po, Sα2, Hb, AIb, Pr and Amy）were investigated by using vertical polyacrylamide gel electrophoresis with discontinuous buffer system. Then the genetic variation during different populations was analyzed. [ Result] Genetic variations were observed in Tf, Sα2 and Po in four populations, others were not polymorphic. There were three alleles at the locus of Tf and Po, two alleles at the loci of Sα2. Effective number of alleles and Nei＇s average expected heterozygosity were 1. 532 4 and 0.230 3 relatively, all higher in Tibetan Mastiff than other populations. [ Conclusion] Protein locus in blood of Tibetan Mastiff existed in genetic variation.

Association between Polymorphisms of MALAT1 and Blood Lead Levels in Lead-exposed Workers

Lead,a heavy metal,which is nonessential but may be harmful to the human body,has been widely used to manufacture many products for use in the modern world.Lead-acid batteries have the advantages of low price,high safety,and advanced technology.

Analysis of the HLA-A,-B allele polymorphism in 5844 umbilical cord blood samples taken from Han population of Shandong province

To investigate the HLA-A, -B allele polymorphism in Han population of Shandong province and to explore the possibility to find out the HLA-A,-B-matehed cord blood donors for stem cell transplantation to be used in other area in China, 5844 umbilical cord blood samples were taken from Han population donors of Shandong province, and assayed with PCR-sequence-oligonucleotide （PCR-SSO） assay. In Shandong Hart donors, 20 alleles at HLA-A locus and 46 alleles at HLA-B locus could be detected as revealed in the present study. Among the 20 alleles at HLA-A locus, the most prevalent five alleles included A ＊ 02（0.3041）, A ＊ 11（0. 1443）, A ＊ 24（0. 1434）, A ＊ 30（0.0975） and A 33（0.0859）, while, the alleles with lower gene frequencies included A ＊ 34（0.0006）, A ＊ 25 （0.0005）, A ＊ 66（0.0005）, A ＊ 74（0.0004） and A ＊ （0.0001）. Of the 46 HLA-B alleles detected, the most prevalent five alleles were B ＊ 13（0.1348）, B ＊ 51（0.0713）, B ＊ 62（0.0712）, B ＊ 61 （0.0676） and B ＊ 60（0.0642）; while alleles with lower gene frequencies included B ＊ 77（0.0001）, B ＊ 76（0.0002）, B ＊ 47（0.0003）, B ＊ 42（0.0003） and B ＊ 72（0.0004）. In comparison with those of the other Han population in China, the HLA-A, -B gene frequencies in the umbilical cord blood of Shandong province possess unique distribution features among the investigated populations from various regions of the same race origin, and the differences in various regions of the same race were less than those among the different race. It is evident that the HLA-A,-B alleles of the umbilical cord blood taken in Shangdong province show high degree of polymorphism, and it might be part of those of Northem Han population in China. So, it is reasonable for patients of Northern Chinese to receive HLA class Ⅰ -match transplant of cord blood stem ceils for tissue and organ transplantation from Shangdong umbilical cord blood bank.

RHD gene polymorphism among RhD-negative Han Chinese

Objective To evaluate the status of eight RHD specific exons in 131 Han Chinese blood donors who were classified as RhD-negative by serological methods and explore the genomic structure of RHD gene among the Han Chinese. The Rh blood group system has the highest prevalence of polymorphisms among human blood group systems and is clinically significant in transfusion medicine. The Rh antigens are expressed on polypeptides encoded by two highly homologous genes,RHD and RHCE. Recent molecular studies have shown that the RhD-negative trait could be generated by multiple genetic mechanisms and is ethnic group-dependent. Methods The polymerase chain reaction using-sequence specific primers (PCR-SSP) was used to amplify exons 2,3,4,5,6,7,9 and 10 of RHD gene and exons 1,2 and 5 of RHCE gene,as well as intron 4 in each of them.Results The 131 cases of RhD-negative phenotypes consisted of 60 ccee,58 Ccee,5 ccEe,5 CcEe and 3 CCee. Among them,83 with the Rh ccee or ccEe phenotypes (63.4%) lacked the eight RHD exons indicated above,while 26 cases with the Rh Ccee,CCee,CcEe phenotypes (19.9%) had all the RHD exons examined. Twenty-two individuals with the Ccee,CCee,CcEe phenotypes (16.8%) carried at least one RHD exon. The phenotypes of the RhD negative individuals carrying the RHD gene were Rh CC or Cc,but not cc. Conclusions Three classes of RhD-negative polymorphisms among a population of Han Chinese were observed. Antigen association analysis suggested the existence of a novel class of RhD-negative associated haplotype in Han Chinese. This haplotype consisted of a normal RHCE allele and a nonfunctional RHD gene. It may be beneficial to redefine the RhD-negative blood group among Chinese populations upon clarification of the mechanisms of RHD gene expression and RhD antigen immunization.

GENETIC VARIATION OF ABO AND RH(D) BLOOD GROUPS AMONG THE BRAHMINS OF COASTAL ANDHRA PRADESH,INDIA

The paper reported the phenotype and allele frequency distribution of ABO and Rh(D) blood groups among Brahmin,an endogamous population from Visakhapatnam district,Andhra Pradesh,India.Blood samples of 100 unrelated Brahmin individuals were screened for ABO and Rh(D) blood groups.The order of occurrence of ABO phenotypes was O>A>B.The corresponding allele frequencies were 0.530,0.315,and 0.155,respectively.The allele frequency of D (0.990) was more than d (0.010).The results were compared with the other caste population to understand the population variations.