A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue...A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue and Brillita. Plant cultures were developed in a controlled environment with 0.4 MPa CO2 enrichment, sucrose-reduced medium, and light intensity of 60 mM m-2·s-1. Principal component analysis showed that component 1 (C1) grouped 64.08% of the total variability, while the first two components accounted for 86.97%. Representation of the principal components demonstrated three clusters corresponding with the blueberry genotypes, and within each cluster plants micropropagated in agar-base medium grouped separately from those plants multiplied in TIBs. Both plant number and total internodes traits (related to the productive efficiency) were demonstrated superior in blueberries propagated in TIBs. Additionally, when transferred to greenhouse conditions, blueberries propagated in TIBs showed higher adaptability and growing rates than those cultured by the conventional approach, altogether evidencing the occurrence of a photomixotrophic stage in the vitroplantlets cultured in TIBs.展开更多
Objective] This study aimed to discuss the key technology of in-vitro cul-ture for a new Vaccinium uliginosum cultivar Zishuijing. [Method] Through the screen-ing and optimization of sterilization method for explants,...Objective] This study aimed to discuss the key technology of in-vitro cul-ture for a new Vaccinium uliginosum cultivar Zishuijing. [Method] Through the screen-ing and optimization of sterilization method for explants, sampling time, multiplication, nursing and rooting culture, a matching clone propagation system was established for the new Vaccinium uliginosum cultivar Zishuijing. [Result] The explants were sterilized with 0.1% HgCl2 for 3 min; the differentiation and multiplication medium of Zishuijing was composed of WPM （modified）, 6-BA （1.0 mg/L） and ZT （1.0 MG/L）; the rootless tube seedlings were transplanted in organic matrix （sawdust∶bark∶peat=1∶1∶1） in September and cultured at air relative humidity of 80%-90% and temperature of 20-25 ℃, and after 50 d, the rooting rate reached 72.4%. [Conclusion] The key technol-ogy of in-vitro culture for the new Vaccinium uliginosum cultivar Zishuijing was estab-lished, thereby providing technical support for large-scale industrialized seedling pro-duction of Zishuijing.展开更多
Background Vaccinium uliginosum L. is a type of blueberry found in the Chinese Changbai Mountains. We extracted Vaccinium uliginosum Anthocyanins （Av.uli） to investigate its bioactivity on suppressing cancer cells. ...Background Vaccinium uliginosum L. is a type of blueberry found in the Chinese Changbai Mountains. We extracted Vaccinium uliginosum Anthocyanins （Av.uli） to investigate its bioactivity on suppressing cancer cells. Methods Av.lli was extracted under different conditions of temperature （10℃-35℃）, pH 1.0-3.0, and diatomaceous earth （1.0 g-3.0 g）, followed by a HPLC analysis for the determination of the ingredients. Its anticancer bioactivities on human colon and colorectal cancer cells （DLD-1 and COLO205） were compared with those on Lonicera caerulea Anthocyanins （AL.cae） and Vaccinium myrtillus Anthocyanins （Av.myr）, using cell viability assays, DNA electrophoresis and nuclear morphology assays. Results The optimum process of Av.uli extraction involved conditions of temperature 20℃, pH 2.0, and diatomaceous earth 1.0 g/50 g of fruit weight. Av.uli contained 5 main components： delphinidin （40.70±1.72）%, cyanidin （3.40±0.68）%, petunidin （17.70±0.54）%, peonidin （2.90±0.63）% and malvidin （35.50±1.11）%. The malvidin percentage was significantly higher （P 〈0.05） than it in Av.myr. Av.uli complied with a dose-dependent repression of cancer cell proliferation with an IC50 （50% inhibitory concentration） value of 50 μg/ml, and showed greater anticancer efficiency than AL. cae and Av. myr under the same cell treatment conditions. These observations were further supported by the results of nuclear assays. Conclusions The extraction protocol and conditions we used were effective for anthocyanin extraction. Av.uli could be a feasible practical research tool and a promising therapeutic source to suppress human colon or colorectal cancers.展开更多
文摘A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue and Brillita. Plant cultures were developed in a controlled environment with 0.4 MPa CO2 enrichment, sucrose-reduced medium, and light intensity of 60 mM m-2·s-1. Principal component analysis showed that component 1 (C1) grouped 64.08% of the total variability, while the first two components accounted for 86.97%. Representation of the principal components demonstrated three clusters corresponding with the blueberry genotypes, and within each cluster plants micropropagated in agar-base medium grouped separately from those plants multiplied in TIBs. Both plant number and total internodes traits (related to the productive efficiency) were demonstrated superior in blueberries propagated in TIBs. Additionally, when transferred to greenhouse conditions, blueberries propagated in TIBs showed higher adaptability and growing rates than those cultured by the conventional approach, altogether evidencing the occurrence of a photomixotrophic stage in the vitroplantlets cultured in TIBs.
基金Supported by Key Project of the National Twelfth-Five Year Research Program of China(2011BAD08B01-03)Funding Project of Department of Forestry of Heilongjiang Province(201004068-6)~~
文摘Objective] This study aimed to discuss the key technology of in-vitro cul-ture for a new Vaccinium uliginosum cultivar Zishuijing. [Method] Through the screen-ing and optimization of sterilization method for explants, sampling time, multiplication, nursing and rooting culture, a matching clone propagation system was established for the new Vaccinium uliginosum cultivar Zishuijing. [Result] The explants were sterilized with 0.1% HgCl2 for 3 min; the differentiation and multiplication medium of Zishuijing was composed of WPM （modified）, 6-BA （1.0 mg/L） and ZT （1.0 MG/L）; the rootless tube seedlings were transplanted in organic matrix （sawdust∶bark∶peat=1∶1∶1） in September and cultured at air relative humidity of 80%-90% and temperature of 20-25 ℃, and after 50 d, the rooting rate reached 72.4%. [Conclusion] The key technol-ogy of in-vitro culture for the new Vaccinium uliginosum cultivar Zishuijing was estab-lished, thereby providing technical support for large-scale industrialized seedling pro-duction of Zishuijing.
文摘Background Vaccinium uliginosum L. is a type of blueberry found in the Chinese Changbai Mountains. We extracted Vaccinium uliginosum Anthocyanins （Av.uli） to investigate its bioactivity on suppressing cancer cells. Methods Av.lli was extracted under different conditions of temperature （10℃-35℃）, pH 1.0-3.0, and diatomaceous earth （1.0 g-3.0 g）, followed by a HPLC analysis for the determination of the ingredients. Its anticancer bioactivities on human colon and colorectal cancer cells （DLD-1 and COLO205） were compared with those on Lonicera caerulea Anthocyanins （AL.cae） and Vaccinium myrtillus Anthocyanins （Av.myr）, using cell viability assays, DNA electrophoresis and nuclear morphology assays. Results The optimum process of Av.uli extraction involved conditions of temperature 20℃, pH 2.0, and diatomaceous earth 1.0 g/50 g of fruit weight. Av.uli contained 5 main components： delphinidin （40.70±1.72）%, cyanidin （3.40±0.68）%, petunidin （17.70±0.54）%, peonidin （2.90±0.63）% and malvidin （35.50±1.11）%. The malvidin percentage was significantly higher （P 〈0.05） than it in Av.myr. Av.uli complied with a dose-dependent repression of cancer cell proliferation with an IC50 （50% inhibitory concentration） value of 50 μg/ml, and showed greater anticancer efficiency than AL. cae and Av. myr under the same cell treatment conditions. These observations were further supported by the results of nuclear assays. Conclusions The extraction protocol and conditions we used were effective for anthocyanin extraction. Av.uli could be a feasible practical research tool and a promising therapeutic source to suppress human colon or colorectal cancers.
