Stroke is a significant leading cause of death and disability in the United States(Tsao et al.,2022).Approximately 87% of strokes fall into the ischemic category,mainly caused by arterial blockage(Jayaraj et al.,2019)...Stroke is a significant leading cause of death and disability in the United States(Tsao et al.,2022).Approximately 87% of strokes fall into the ischemic category,mainly caused by arterial blockage(Jayaraj et al.,2019).Although the only FDA-approved effective medication is tissue plasminogen activator(tPA),it should be administrated within 4.5 hours of ischemic stroke.Furthermore,tPA has been an integral part of managing acute ischemic stro ke.展开更多
Objective:To study the correlation of renal arterial ultrasound parameters with renal damage and placental hypoxia in patients with preeclampsia.Methods: A total of 56 cases of pregnant women who were diagnosed with p...Objective:To study the correlation of renal arterial ultrasound parameters with renal damage and placental hypoxia in patients with preeclampsia.Methods: A total of 56 cases of pregnant women who were diagnosed with preeclampsia in Mianyang People's Hospital between May 2014 and October 2016 were selected as the PE group of the research, and healthy pregnant women who received antenatal care during the same period were selected as the control group. Color Doppler diasonograph was used to determine renal arterial blood flow parameters, and enzyme-linked immunosorbent assay kits were used to determine the contents of renal damage and endothelial injury indexes in serum as well as the contents of apoptosis molecules caused by hypoxia in placenta.Results: Renal interlobar arterial Vs, Vd and AT levels in PE group were significantly higher than those in control group;CysC,β2-MG, sFlt-1, sEng, ET-1, AnnexinV and vWF contents in serum as well as KIM-1 and NGAL contents in urine of PE group were significantly higher than those of control group and positively correlated with renal interlobar arterial Vs, Vd and AT levels, and the ROS, CytC, Caspase-3 and Caspase-9 contents in placenta were significantly higher than those of control group and positively correlated with renal interlobar arterial Vs, Vd and AT levels.Conclusion: The change of renal arterial blood flow parameters in patients with preeclampsia can assess the extent of renal damage and placental hypoxia.展开更多
AIM: To evaluate the effects of extrahepatic collaterals to the liver on liver damage and patient outcome after embolotherapy for the ruptured hepatic artery pseudoa- neurysm following hepatobiliary pancreatic surgery...AIM: To evaluate the effects of extrahepatic collaterals to the liver on liver damage and patient outcome after embolotherapy for the ruptured hepatic artery pseudoa- neurysm following hepatobiliary pancreatic surgery. METHODS: We reviewed 9 patients who underwent transcatheter arterial embolization (TAE) for the ruptured hepatic artery pseudoaneurysm following major hepato- biliary pancreatic surgery between June 1992 and April 2006. We paid special attention to the extrahepatic arte- rial collaterals to the liver which may affect post-TAE liver damage and patient outcome. RESULTS: The underlying diseases were all malignan- cies, and the surgical procedures included hepatopancre- atoduodenectomy in 2 patients, hepatic resection with removal of the bile duct in 5, and pancreaticoduodenec- tomy in 2. A total of 11 pseudoaneurysm developed: 4 in the common hepatic artery, 4 in the proper hepatic artery, and 3 in the right hepatic artery. Successful he- mostasis was accomplished with the initial TAE in all patients, except for 1. Extrahepatic arterial pathways to the liver, including the right inferior phrenic artery, the jejunal branches, and the aberrant left hepatic artery, were identified in 8 of the 9 patients after the completion of TAE. The development of collaterals depended on the extent of liver mobilization during the hepatic resection, the postoperative period, the presence or absence of an aberrant left hepatic artery, and the concomitant arte- rial stenosis adjacent to the pseudoaneurysm. The liver tolerated TAE without significant consequences when at least one of the collaterals from the inferior phrenic ar-tery or the aberrant left hepatic artery was present. One patient, however, with no extrahepatic collaterals died of liver failure due to total liver necrosis 9 d after TAE. CONCLUSION: When TAE is performed on ruptured hepatic artery pseudoaneurysm, reduced collateral path- ways to the liver created by the primary surgical proce- dure and a short postoperative interval may lead to an unfavorable outcome.展开更多
The Chinese compound Kaixin fieyu Fang can be used to treat vascular depression; however, the underlying mechanism remains unclear. This study established a rat model of chronic cerebral ischemia-caused white matter d...The Chinese compound Kaixin fieyu Fang can be used to treat vascular depression; however, the underlying mechanism remains unclear. This study established a rat model of chronic cerebral ischemia-caused white matter damage by ligation of the bilateral common carotid arteries. Rats received daily intragastric administration of a suspension of Kaixin ]ieyu Fang powder. After 3, 7 and 21 days of treatment, the degree of white matter damage in the cerebral ischemia rat model was alleviated, Bcl-2 protein and mRNA expression in brain tissue increased, and Bax protein and mRNA expression decreased. These results indicate that Kaixin Jieyu Fang can alleviate cere- bral white matter damage, and the underlying mechanism is associated with regulation of Bcl-2/ Bax protein and mRNA expression, which is one of possible mechanism behind the protective effect of Kaixin Jieyu Fang against vascular depression.展开更多
Cheonggukjang is a soybean paste made by fermenting whole cooked soybeans with Bacillus subtilis. Cheonggukjang contains a fibrinolytic enzyme that could provide clinical applications for removing blood clots. In the ...Cheonggukjang is a soybean paste made by fermenting whole cooked soybeans with Bacillus subtilis. Cheonggukjang contains a fibrinolytic enzyme that could provide clinical applications for removing blood clots. In the present study, the term ＂cheonggukjang kinase＂ （CGK） was used to refer to this fibrinolytic enzyme. The thrombolytic effects of CGK were analyzed in a rat model of cerebral embolic stroke produced by middle cerebral artery occlusion （MCAO）. Results from fibrin and platelet-rich clot lysis assays demonstrated that thrombolytic activity was greatest in CGKs, which were cultured for 40 hours. In addition, T50, the time needed to decompose 50% of the clot, did not change with plasminogen treatment, indicating that CGK was not a plasminogen activator, but was rather presumed to act as a plasmin-like protein. An intravenous infusion of CGK （1 U plasmin-like activity/100 μg CGK/kg） at 1 hour after MCAO resulted in removal of clots in a rat model of cerebral embolic stroke. CGK-treated groups exhibited a significant dose-dependent reduction in infarct volume. CGK treatment also improved functional recovery, as assessed by neurological deficit scores. Decreased infarct volume and improved functional recovery following CGK treatment was greater compared with recombinant tissue plasminogen activator （10 mg/kg）. These results suggested that CGK effectively reduced infarct volume and improved functional recovery following ischemic brain injury. CGK exhibits a number of potential clinical applications ir the treatment of cerebral embolic stroke.展开更多
目的探讨急性脑缺血/再灌注大鼠神经元细胞骨架蛋白时空动态变化。方法线栓法阻断大鼠大脑中动脉90 min后实现再灌注,在不同再灌注时间点观察及取材。尼氏染色观察神经细胞损伤,采用神经功能缺损评分和前肢放置实验评估神经功能;免疫组...目的探讨急性脑缺血/再灌注大鼠神经元细胞骨架蛋白时空动态变化。方法线栓法阻断大鼠大脑中动脉90 min后实现再灌注,在不同再灌注时间点观察及取材。尼氏染色观察神经细胞损伤,采用神经功能缺损评分和前肢放置实验评估神经功能;免疫组化染色、免疫印迹法观察细胞骨架成分微管相关蛋白2(microtubule associated protein 2,MAP2)、神经丝重链(neurofilament heavy chain,NF-H)的变化;透射电镜观察轴突、树突和神经丝亚显微结构。结果随着再灌注时间的延长,脑损伤和神经行为功能损害逐渐加重。纹状体损伤比皮层出现的更早、更严重。缺血区域的MAP2相关免疫反应强度降低,NF-H相关免疫反应强度升高。超微结构观察显示细胞骨架排列受损,密度降低。结论不同脑区对缺血/再灌注损伤的耐受性不同。神经元细胞骨架的主要成分对缺血和再灌注表现出动态反应,这可能进一步促进脑损伤和神经功能缺损。展开更多
基金supported by the UTHSC Bridge funding award (E073005058 Bridge Support-2022)the National Institute of Health (R01-NS09 7800 and R56 NS127924-01) to TI。
文摘Stroke is a significant leading cause of death and disability in the United States(Tsao et al.,2022).Approximately 87% of strokes fall into the ischemic category,mainly caused by arterial blockage(Jayaraj et al.,2019).Although the only FDA-approved effective medication is tissue plasminogen activator(tPA),it should be administrated within 4.5 hours of ischemic stroke.Furthermore,tPA has been an integral part of managing acute ischemic stro ke.
文摘Objective:To study the correlation of renal arterial ultrasound parameters with renal damage and placental hypoxia in patients with preeclampsia.Methods: A total of 56 cases of pregnant women who were diagnosed with preeclampsia in Mianyang People's Hospital between May 2014 and October 2016 were selected as the PE group of the research, and healthy pregnant women who received antenatal care during the same period were selected as the control group. Color Doppler diasonograph was used to determine renal arterial blood flow parameters, and enzyme-linked immunosorbent assay kits were used to determine the contents of renal damage and endothelial injury indexes in serum as well as the contents of apoptosis molecules caused by hypoxia in placenta.Results: Renal interlobar arterial Vs, Vd and AT levels in PE group were significantly higher than those in control group;CysC,β2-MG, sFlt-1, sEng, ET-1, AnnexinV and vWF contents in serum as well as KIM-1 and NGAL contents in urine of PE group were significantly higher than those of control group and positively correlated with renal interlobar arterial Vs, Vd and AT levels, and the ROS, CytC, Caspase-3 and Caspase-9 contents in placenta were significantly higher than those of control group and positively correlated with renal interlobar arterial Vs, Vd and AT levels.Conclusion: The change of renal arterial blood flow parameters in patients with preeclampsia can assess the extent of renal damage and placental hypoxia.
文摘AIM: To evaluate the effects of extrahepatic collaterals to the liver on liver damage and patient outcome after embolotherapy for the ruptured hepatic artery pseudoa- neurysm following hepatobiliary pancreatic surgery. METHODS: We reviewed 9 patients who underwent transcatheter arterial embolization (TAE) for the ruptured hepatic artery pseudoaneurysm following major hepato- biliary pancreatic surgery between June 1992 and April 2006. We paid special attention to the extrahepatic arte- rial collaterals to the liver which may affect post-TAE liver damage and patient outcome. RESULTS: The underlying diseases were all malignan- cies, and the surgical procedures included hepatopancre- atoduodenectomy in 2 patients, hepatic resection with removal of the bile duct in 5, and pancreaticoduodenec- tomy in 2. A total of 11 pseudoaneurysm developed: 4 in the common hepatic artery, 4 in the proper hepatic artery, and 3 in the right hepatic artery. Successful he- mostasis was accomplished with the initial TAE in all patients, except for 1. Extrahepatic arterial pathways to the liver, including the right inferior phrenic artery, the jejunal branches, and the aberrant left hepatic artery, were identified in 8 of the 9 patients after the completion of TAE. The development of collaterals depended on the extent of liver mobilization during the hepatic resection, the postoperative period, the presence or absence of an aberrant left hepatic artery, and the concomitant arte- rial stenosis adjacent to the pseudoaneurysm. The liver tolerated TAE without significant consequences when at least one of the collaterals from the inferior phrenic ar-tery or the aberrant left hepatic artery was present. One patient, however, with no extrahepatic collaterals died of liver failure due to total liver necrosis 9 d after TAE. CONCLUSION: When TAE is performed on ruptured hepatic artery pseudoaneurysm, reduced collateral path- ways to the liver created by the primary surgical proce- dure and a short postoperative interval may lead to an unfavorable outcome.
基金supported by the National Natural Science Foundation of China,No.30672696,81072801the Natural Science Foundation of Beijing in China,No.7093129
文摘The Chinese compound Kaixin fieyu Fang can be used to treat vascular depression; however, the underlying mechanism remains unclear. This study established a rat model of chronic cerebral ischemia-caused white matter damage by ligation of the bilateral common carotid arteries. Rats received daily intragastric administration of a suspension of Kaixin ]ieyu Fang powder. After 3, 7 and 21 days of treatment, the degree of white matter damage in the cerebral ischemia rat model was alleviated, Bcl-2 protein and mRNA expression in brain tissue increased, and Bax protein and mRNA expression decreased. These results indicate that Kaixin Jieyu Fang can alleviate cere- bral white matter damage, and the underlying mechanism is associated with regulation of Bcl-2/ Bax protein and mRNA expression, which is one of possible mechanism behind the protective effect of Kaixin Jieyu Fang against vascular depression.
基金the National Research Foundation of Korea,funded by the Korean Ministry of Education,Sci-ence and Technology,No.2007-0055330Inje Univer-sity Research Grant (2008)
文摘Cheonggukjang is a soybean paste made by fermenting whole cooked soybeans with Bacillus subtilis. Cheonggukjang contains a fibrinolytic enzyme that could provide clinical applications for removing blood clots. In the present study, the term ＂cheonggukjang kinase＂ （CGK） was used to refer to this fibrinolytic enzyme. The thrombolytic effects of CGK were analyzed in a rat model of cerebral embolic stroke produced by middle cerebral artery occlusion （MCAO）. Results from fibrin and platelet-rich clot lysis assays demonstrated that thrombolytic activity was greatest in CGKs, which were cultured for 40 hours. In addition, T50, the time needed to decompose 50% of the clot, did not change with plasminogen treatment, indicating that CGK was not a plasminogen activator, but was rather presumed to act as a plasmin-like protein. An intravenous infusion of CGK （1 U plasmin-like activity/100 μg CGK/kg） at 1 hour after MCAO resulted in removal of clots in a rat model of cerebral embolic stroke. CGK-treated groups exhibited a significant dose-dependent reduction in infarct volume. CGK treatment also improved functional recovery, as assessed by neurological deficit scores. Decreased infarct volume and improved functional recovery following CGK treatment was greater compared with recombinant tissue plasminogen activator （10 mg/kg）. These results suggested that CGK effectively reduced infarct volume and improved functional recovery following ischemic brain injury. CGK exhibits a number of potential clinical applications ir the treatment of cerebral embolic stroke.
文摘目的探讨急性脑缺血/再灌注大鼠神经元细胞骨架蛋白时空动态变化。方法线栓法阻断大鼠大脑中动脉90 min后实现再灌注,在不同再灌注时间点观察及取材。尼氏染色观察神经细胞损伤,采用神经功能缺损评分和前肢放置实验评估神经功能;免疫组化染色、免疫印迹法观察细胞骨架成分微管相关蛋白2(microtubule associated protein 2,MAP2)、神经丝重链(neurofilament heavy chain,NF-H)的变化;透射电镜观察轴突、树突和神经丝亚显微结构。结果随着再灌注时间的延长,脑损伤和神经行为功能损害逐渐加重。纹状体损伤比皮层出现的更早、更严重。缺血区域的MAP2相关免疫反应强度降低,NF-H相关免疫反应强度升高。超微结构观察显示细胞骨架排列受损,密度降低。结论不同脑区对缺血/再灌注损伤的耐受性不同。神经元细胞骨架的主要成分对缺血和再灌注表现出动态反应,这可能进一步促进脑损伤和神经功能缺损。
