Boehmeria nivea var.strigosa Zeng Y.Wu&Y.Zhao,a new variety of B.nivea(Urticaceae)from Southwest China,is here described based on evidence from morphology and molecular phylogeny.This new variety is mainly charact...Boehmeria nivea var.strigosa Zeng Y.Wu&Y.Zhao,a new variety of B.nivea(Urticaceae)from Southwest China,is here described based on evidence from morphology and molecular phylogeny.This new variety is mainly characterized by its green abaxial leaf blade,partly connate stipules,and densely patent strigose hairs on stems and potioles.The phylogenetic analysis based on rbc L,nrDNA and rbc L+nrDNA datasets,revealed that all individuals of B.nivea var.strigosa formed a monophyletic group.The conservation status of B.nivea var.strigosa is assessed as“Near Threatened”(NT)according to IUCN evaluation criteria.The discovery of this new variety is not only crucial for the taxonomy of ramie,but also provides reference for the exploration and utilization of ramie.展开更多
AIM: To explore the anti-hepatitis B virus (HBV) effects of Boehmeria nivea (B. nivea) root extract (BNE) by using the HepG2 2.2.15 cell model system. METHODS: Hepatitis B surface antigen (HBsAg), hepatitis B virus e ...AIM: To explore the anti-hepatitis B virus (HBV) effects of Boehmeria nivea (B. nivea) root extract (BNE) by using the HepG2 2.2.15 cell model system. METHODS: Hepatitis B surface antigen (HBsAg), hepatitis B virus e antigen (HBeAg), and HBV DNA were measured by using ELISA and real-time PCR, respectively. Viral DNA replication and RNA expression were determined by using Southern and Northern blot, respectively. RESULTS: In HepG2 2.2.15 cells, HBeAg (60%, P < 0.01) and particle-associated HBV DNA (> 99%, P < 0.01) secretion into supernatant were significantly inhibited by BNE at a dose of 100 mg/L, whereas the HBsAg was not inhibited. With different doses of BNE, the reduced HBeAg was correlated with the inhibition of HBV DNA. The anti-HBV effect of BNE was not caused by its cytotoxicity to cells or inhibition of viral DNA replication and RNA expression. CONCLUSION: BNE could effectively reduce the HBV production and its anti-HBV machinery might differ from the nucleoside analogues.展开更多
Six male sterile lines (MSLs) of ramie (Boeh-meria nivea (L.) Gaud.) were grown in dark rooms under the photoperiods of 9.5h, 11h or 12.5h d-1 in spring and summer seasons (dif-ferent environmental temperatures) to te...Six male sterile lines (MSLs) of ramie (Boeh-meria nivea (L.) Gaud.) were grown in dark rooms under the photoperiods of 9.5h, 11h or 12.5h d-1 in spring and summer seasons (dif-ferent environmental temperatures) to test their developmental response to photoperiod and temperature. The MSLs showed little difference in vegetative growth duration, but different de-velopment rates in the reproductive growth stage under the tested conditions. Higher tem-perature (grown in summer) mainly accelerated vegetative growth, while the short photoperiod treatment accelerated the reproductive growth of the MSLs. Moreover, the short photoperiod treatment combined with higher temperature obviously accelerated both the vegetative and reproductive growth of the MSLs. But the effect of higher temperature decreased, or even dis-appeared along with the photoperiod elongation. The MSLs were divided into 5 photo-temperature response types, based on the flower budding acceleration of short pho-toperiod and the approximate temperature re-sponse index.展开更多
By using cpSSR （chloroplast microsatellites） markers, the genetic relationship among Boehmeria varieties was analyzed. Five pairs of cpSSR primers with excellent amplification result, clear amplification band and go...By using cpSSR （chloroplast microsatellites） markers, the genetic relationship among Boehmeria varieties was analyzed. Five pairs of cpSSR primers with excellent amplification result, clear amplification band and good repeatability were screened from 22 pairs of cpSSR primers, and the polymorphism rate was 22.73%. By using these five pairs of cpSSR primers, 16 polymorphic loci were amplified from eight experimental materials, with an average of 3.2 loci. According to the results of duster analysis, these experimental materials were divided into three categories. The clustering results were consistent with the classification results based on chloroplast gene sequences.展开更多
Rhizosphere microbial network in crater had higher complexity than in volcanic cone.Bacteria were more prone to enrichment than fungi in volcanic soils.The bacteria exhibited greater resistance and resilience than fun...Rhizosphere microbial network in crater had higher complexity than in volcanic cone.Bacteria were more prone to enrichment than fungi in volcanic soils.The bacteria exhibited greater resistance and resilience than fungi.Volcanic eruptions are significant natural disturbances that provide valuable opportunities to study their impacts on soil microorganisms.However,no previous studies have compared the rhizosphere microbial communities of Boehmeria nivea L.in volcanic craters and cones.To address this gap,we conducted a comprehensive investigation using Illumina MiSeq high-throughput sequencing to compare the rhizosphere microbial communities in volcanic craters and cones.Principal Coordinate Analysis revealed significant differences in the rhizosphere microbial communities between the crater and cone.The bacterial communities in the rhizosphere of the crater exhibited higher diversity and evenness compared to the cones.Moreover,the cones displayed more intricate bacterial networks than the crater(nodes 556 vs.440).Conversely,fungal networks were more complex in the crater than the cone(nodes 943 vs.967).Additionally,bacterial communities demonstrated greater stability than fungal ones within these volcanic soils(avgK 241.1 vs.499.7)and(avgCC 1.047 vs.1.092).Furthermore,the Structural Equation Model demonstrated a direct positive impact of alpha diversity on soil microbial community multifunctionality in the crater(λ=0.920,P<0.001).Our findings have presented the opportunity to investigate the characteristics of the rhizosphere microbial communities of Boehmeria nivea L.in the crater and cone.展开更多
文摘Boehmeria nivea var.strigosa Zeng Y.Wu&Y.Zhao,a new variety of B.nivea(Urticaceae)from Southwest China,is here described based on evidence from morphology and molecular phylogeny.This new variety is mainly characterized by its green abaxial leaf blade,partly connate stipules,and densely patent strigose hairs on stems and potioles.The phylogenetic analysis based on rbc L,nrDNA and rbc L+nrDNA datasets,revealed that all individuals of B.nivea var.strigosa formed a monophyletic group.The conservation status of B.nivea var.strigosa is assessed as“Near Threatened”(NT)according to IUCN evaluation criteria.The discovery of this new variety is not only crucial for the taxonomy of ramie,but also provides reference for the exploration and utilization of ramie.
文摘AIM: To explore the anti-hepatitis B virus (HBV) effects of Boehmeria nivea (B. nivea) root extract (BNE) by using the HepG2 2.2.15 cell model system. METHODS: Hepatitis B surface antigen (HBsAg), hepatitis B virus e antigen (HBeAg), and HBV DNA were measured by using ELISA and real-time PCR, respectively. Viral DNA replication and RNA expression were determined by using Southern and Northern blot, respectively. RESULTS: In HepG2 2.2.15 cells, HBeAg (60%, P < 0.01) and particle-associated HBV DNA (> 99%, P < 0.01) secretion into supernatant were significantly inhibited by BNE at a dose of 100 mg/L, whereas the HBsAg was not inhibited. With different doses of BNE, the reduced HBeAg was correlated with the inhibition of HBV DNA. The anti-HBV effect of BNE was not caused by its cytotoxicity to cells or inhibition of viral DNA replication and RNA expression. CONCLUSION: BNE could effectively reduce the HBV production and its anti-HBV machinery might differ from the nucleoside analogues.
文摘Six male sterile lines (MSLs) of ramie (Boeh-meria nivea (L.) Gaud.) were grown in dark rooms under the photoperiods of 9.5h, 11h or 12.5h d-1 in spring and summer seasons (dif-ferent environmental temperatures) to test their developmental response to photoperiod and temperature. The MSLs showed little difference in vegetative growth duration, but different de-velopment rates in the reproductive growth stage under the tested conditions. Higher tem-perature (grown in summer) mainly accelerated vegetative growth, while the short photoperiod treatment accelerated the reproductive growth of the MSLs. Moreover, the short photoperiod treatment combined with higher temperature obviously accelerated both the vegetative and reproductive growth of the MSLs. But the effect of higher temperature decreased, or even dis-appeared along with the photoperiod elongation. The MSLs were divided into 5 photo-temperature response types, based on the flower budding acceleration of short pho-toperiod and the approximate temperature re-sponse index.
基金Supported by Fund for Basic Scientific Research and Operating Expenses of Chinese Academy Of Agricultural Sciences(1610032012030)
文摘By using cpSSR （chloroplast microsatellites） markers, the genetic relationship among Boehmeria varieties was analyzed. Five pairs of cpSSR primers with excellent amplification result, clear amplification band and good repeatability were screened from 22 pairs of cpSSR primers, and the polymorphism rate was 22.73%. By using these five pairs of cpSSR primers, 16 polymorphic loci were amplified from eight experimental materials, with an average of 3.2 loci. According to the results of duster analysis, these experimental materials were divided into three categories. The clustering results were consistent with the classification results based on chloroplast gene sequences.
基金supported by the Key Research and Development Program of Anhui Province(Grant No.202204c06020021)the National Key Research and Development Program of China(Grant No.2023YFD1901002)the National Natural Science Foundation of China(Grant No.32201308).
文摘Rhizosphere microbial network in crater had higher complexity than in volcanic cone.Bacteria were more prone to enrichment than fungi in volcanic soils.The bacteria exhibited greater resistance and resilience than fungi.Volcanic eruptions are significant natural disturbances that provide valuable opportunities to study their impacts on soil microorganisms.However,no previous studies have compared the rhizosphere microbial communities of Boehmeria nivea L.in volcanic craters and cones.To address this gap,we conducted a comprehensive investigation using Illumina MiSeq high-throughput sequencing to compare the rhizosphere microbial communities in volcanic craters and cones.Principal Coordinate Analysis revealed significant differences in the rhizosphere microbial communities between the crater and cone.The bacterial communities in the rhizosphere of the crater exhibited higher diversity and evenness compared to the cones.Moreover,the cones displayed more intricate bacterial networks than the crater(nodes 556 vs.440).Conversely,fungal networks were more complex in the crater than the cone(nodes 943 vs.967).Additionally,bacterial communities demonstrated greater stability than fungal ones within these volcanic soils(avgK 241.1 vs.499.7)and(avgCC 1.047 vs.1.092).Furthermore,the Structural Equation Model demonstrated a direct positive impact of alpha diversity on soil microbial community multifunctionality in the crater(λ=0.920,P<0.001).Our findings have presented the opportunity to investigate the characteristics of the rhizosphere microbial communities of Boehmeria nivea L.in the crater and cone.
