The Experimental Study of Constructing Tissue Engineered Bone by Compounding Zinc-sintered Bovine Cancellous Bone with Marrow Stromal Cells

To study the osteogenic ability of tissue-engineered bone constructed by compounding zinc-sintered bovine cancellous bone with rabbit marrow stromal cells (MSCs) in vivo,the zinc-sintered bovine cancellous bone of beta-tricalcium phosphate (TCP) type was prepared by sintering the fresh calf cancellous bone twice and then loading it with zinc-ion.The rabbit MSCs were cultured,induced and seeded onto the zinc-sintered bovine cancellous bones.The tissue-engineered bones were then implanted into the rabbits' back muscles.The newly formed bone tissues were observed by histological methods and the areas of new osseous tissues were measured at the end of the 4th and 8th week.The zinc-sintered bovine cancellous bones alone were implanted on the other side as control.The osteogenic activity of MSCs was identified by alkaline phosphatase (ALP) staining and calcification nod chinalizarin staining.At the end of 4th week,a small amount of new bone tissues was observed.At the end of 8th week,there were many newly formed bone mature tissues.Moreover,the area of the latter was significantly larger than that of the former(P<0.01),while in the control group there was no new bone formation.The tissue-engineered bone,which was constructed by combining zinc-sintered bovine cancellous bone with MSCs,has satisfactory osteogenic capabilities in vivo.

血清EGF及TRACP-5b水平在乳腺癌患者骨转移中的应用价值

目的:探讨血清EGF及TRACP-5b水平在早期诊断乳腺癌骨转移的应用价值。方法:定量检测75例乳腺癌患者和30例健康女性献血员血清中EGF及TRACP-5b含量,应用SPECT核素骨扫描确定有无骨转移。结果:伴骨转移的乳腺癌患者血清EGF及TRACP-5b水平显著高于非骨转移组,差异有统计学意义(P<0.05)。两组患者的血清EGF及TRACP-5b水平显著高于正常对照组,差异有统计学意义(P<0.05)。结论:联合检测骨转换指标EGF及TRACP-5b在乳腺癌骨转移的早期诊断及病程监测中有重要应用价值。目的:探讨血清EGF及TRACP-5b水平在早期诊断乳腺癌骨转移的应用价值。方法:定量检测75例乳腺癌患者和30例健康女性献血员血清中EGF及TRACP-5b含量,应用SPECT核素骨扫描确定有无骨转移。结果:伴骨转移的乳腺癌患者血清EGF及TRACP-5b水平显著高于非骨转移组,差异有统计学意义(P<0.05)。两组患者的血清EGF及TRACP-5b水平显著高于正常对照组,差异有统计学意义(P<0.05)。结论:联合检测骨转换指标EGF及TRACP-5b在乳腺癌骨转移的早期诊断及病程监测中有重要应用价值。

骨炭对水中不同形态Sb吸附和解吸的影响

采用骨炭作为吸附剂，研究其在不同骨炭用量、pH值和温度条件下对水中Sb（Ⅲ）和Sb（Ⅴ）吸附和解吸的影响。结果表明，骨炭对Sb（Ⅲ）的吸附效果远好于Sb（V），在0．2～8．0mmol／L的Sb（Ⅲ）和Sb（Ⅴ）浓度下，骨炭对这2种形态Sb的去除率分别为46．1％～78．6％和9．6％～31．7％。采用Langmuir方程和Freundlich方程均可以很好地拟合骨炭对Sb（Ⅲ）和Sb（Ⅴ）的吸附，Sb（Ⅲ）和Sb（Ⅴ）的最大吸附容量分别为110．1034mg/g和17．4167mg／g。骨炭对Sb（Ⅲ）的解吸也大于对Sb（Ⅴ）的解吸。骨炭对Sb（Ⅲ）和Sb（Ⅴ）的吸附受不同骨炭用量、pH值和温度影响。

WNT激活剂对人骨髓基质干细胞成脂-成骨分化的影响

目的观察WNT信号通路激活剂SB-216763对于人骨髓基质干细胞（hBMSCs）成脂．成骨分化的影响。方法从3例临床股骨头标本中分离培养hBMSCs与5μmol／LSB-216763共同培养，观察hBMSCs表达β-连锁蛋白（β-catenin）的变化，检测hBMSCs增殖能力、成脂分化能力和成骨细胞诱导分化时碱性磷酸酶（ALP）活性的变化。结果SB-216763可以显著促进hBMSCs表达β-catenin（30．2±2．7比13．3±2．1，P〈0．01）、促进hBMSCs增殖（P〈0．01）、抑制hBMSCs向脂肪细胞分化[油红O（OilRed—O）阳性细胞数：27．4±3．2比8．4±2．1，P〈0．01]、降低hBMSEs的ALP活性（P〈0．01）。结论激活WNT信号通路可以促进hBMSCs的增殖，抑制hBMSCs向脂肪细胞分化并且抑制hBMSCs的成骨活性。