AIM:To establish a Chinese esophageal squamous cell carcinoma(ESCC)cell line with high bone metastasis potency using99mTc-methylene diphosphonate(99mTcMDP)micro-pinhole scintigraphy,X ray and micropositron emission to...AIM:To establish a Chinese esophageal squamous cell carcinoma(ESCC)cell line with high bone metastasis potency using99mTc-methylene diphosphonate(99mTcMDP)micro-pinhole scintigraphy,X ray and micropositron emission tomography/computed tomography(PET/CT)for exploring the mechanism of occurrence and development in esophageal cancer.METHODS:The cells came from a BALB/c nu/nu immunodeficient mouse,and oncogenic tumor tissue was from a surgical specimen from a 61-year-old male patient with ESCC.The cell growth curve was mapped and analysis of chromosome karyotype was performed.Approximately 1×106oncogenic cells were injected into the left cardiac ventricle of immunodeficient mice.The bone metastatic lesions of tumor-bearing mice were detected by99mTc-MDP scintigraphy,micro-PET/CT and X-ray,and were resected from the mice under deep anesthesia.The bone metastatic cells in the lesions were used for culture and for repeated intracardiac inoculation.This in vivo/in vitro experimental metastasis study was repeated for four cycles.All of the suspicious bone sites were confirmed by pathology.Real-time polymerase chain reaction was used to compare the gene expression in the parental cells and in the bone metastatic clone.RESULTS:The surgical specimen was implanted subcutaneously in immunodeficient mice and the tumorigenesis rate was 100%.First-passage oncogenic cells were named CEK-Sq-1.The chromosome karyotype analysis of the cell line was hypotriploid.The bone metastasis rate went from 20%with the first-passage oncogenic cells via intracardiac inoculation to 90%after four cycles.The established bone metastasis clone named CEK-Sq-1BM had a high potential to metastasize in bone,including mandible,humerus,thoracic and lumbar vertebrae,scapula and femur.The bone metastasis lesions were successfully detected by micro-pinhole bone scintigraphy,micro-PET/CT,and X-ray.The sensitivity,specificity and accuracy of the micro-pinhole scintigraphy,X-ray,and micro-PET/CT imaging examinations were:89.66%/32%/80%,88.2%/100%/89.2%,and 88.75%/77.5%/87.5%,respectively.Some gene expression difference was found between parental and bone metastasis cells.CONCLUSION:This newly established Chinese ESCC cell line and animal model may provide a useful tool for the study of the pathogenesis and development of esophageal carcinoma.展开更多
Continuous expansion of rat neural stem cell lines has not been achieved due to proliferation arrest and spontaneous differentiation in vitro. In the current study, neural precursor cells derived from the subventricul...Continuous expansion of rat neural stem cell lines has not been achieved due to proliferation arrest and spontaneous differentiation in vitro. In the current study, neural precursor cells derived from the subventricular zone of adult rats spontaneously underwent astroglial and oligodendroglial differentiation after limited propagation. This differentiation was largely induced by autocrine or paracrine bone morphogenetic protein and platelet derived growth factor signals. The results showed that, by inhibiting bone morphogenetic protein and platelet derived growth factor signals, adult rat neural precursor cells could be extensively cultured in vitro as tripotent stem cell lines. In addition to adult rat neural stem cells, we found that bone morphogenetic protein antagonists can promote the proliferation of human neural stem cells. Therefore, the present findings illustrated the role of autocrine or paracrine bone morphogenetic protein and platelet derived growth factor signaling in determining neural stem cell self-renewal and differentiation. By antagonizing both signals, the long-term propagation of rat neural stem cell lines can be achieved.展开更多
基金Supported by Shanghai Science and Technology fundamental research Grant 08140902202 and 09140901500(to Yang SF)National Natural Science Foundation of China Grant 30973017(to Yang QC)
文摘AIM:To establish a Chinese esophageal squamous cell carcinoma(ESCC)cell line with high bone metastasis potency using99mTc-methylene diphosphonate(99mTcMDP)micro-pinhole scintigraphy,X ray and micropositron emission tomography/computed tomography(PET/CT)for exploring the mechanism of occurrence and development in esophageal cancer.METHODS:The cells came from a BALB/c nu/nu immunodeficient mouse,and oncogenic tumor tissue was from a surgical specimen from a 61-year-old male patient with ESCC.The cell growth curve was mapped and analysis of chromosome karyotype was performed.Approximately 1×106oncogenic cells were injected into the left cardiac ventricle of immunodeficient mice.The bone metastatic lesions of tumor-bearing mice were detected by99mTc-MDP scintigraphy,micro-PET/CT and X-ray,and were resected from the mice under deep anesthesia.The bone metastatic cells in the lesions were used for culture and for repeated intracardiac inoculation.This in vivo/in vitro experimental metastasis study was repeated for four cycles.All of the suspicious bone sites were confirmed by pathology.Real-time polymerase chain reaction was used to compare the gene expression in the parental cells and in the bone metastatic clone.RESULTS:The surgical specimen was implanted subcutaneously in immunodeficient mice and the tumorigenesis rate was 100%.First-passage oncogenic cells were named CEK-Sq-1.The chromosome karyotype analysis of the cell line was hypotriploid.The bone metastasis rate went from 20%with the first-passage oncogenic cells via intracardiac inoculation to 90%after four cycles.The established bone metastasis clone named CEK-Sq-1BM had a high potential to metastasize in bone,including mandible,humerus,thoracic and lumbar vertebrae,scapula and femur.The bone metastasis lesions were successfully detected by micro-pinhole bone scintigraphy,micro-PET/CT,and X-ray.The sensitivity,specificity and accuracy of the micro-pinhole scintigraphy,X-ray,and micro-PET/CT imaging examinations were:89.66%/32%/80%,88.2%/100%/89.2%,and 88.75%/77.5%/87.5%,respectively.Some gene expression difference was found between parental and bone metastasis cells.CONCLUSION:This newly established Chinese ESCC cell line and animal model may provide a useful tool for the study of the pathogenesis and development of esophageal carcinoma.
基金the National Natural Science Foundation of China,No.81000518China Postdoctoral Science Foundation,No.201003237+2 种基金the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry of ChinaShanghai Pujiang Program by Science and Technology Commission of Shanghai Municipality,No. 09PJ1408300Key Basic Research Project by Science and Technology Commission of Shanghai Municipality,No. 10JC1402300
文摘Continuous expansion of rat neural stem cell lines has not been achieved due to proliferation arrest and spontaneous differentiation in vitro. In the current study, neural precursor cells derived from the subventricular zone of adult rats spontaneously underwent astroglial and oligodendroglial differentiation after limited propagation. This differentiation was largely induced by autocrine or paracrine bone morphogenetic protein and platelet derived growth factor signals. The results showed that, by inhibiting bone morphogenetic protein and platelet derived growth factor signals, adult rat neural precursor cells could be extensively cultured in vitro as tripotent stem cell lines. In addition to adult rat neural stem cells, we found that bone morphogenetic protein antagonists can promote the proliferation of human neural stem cells. Therefore, the present findings illustrated the role of autocrine or paracrine bone morphogenetic protein and platelet derived growth factor signaling in determining neural stem cell self-renewal and differentiation. By antagonizing both signals, the long-term propagation of rat neural stem cell lines can be achieved.
