Objective： Cheongseoikki-tang （CIT, Korean）, also called Qingshu Yiqi decoction（清署益气汤）and Seisho-ekki-to （Japanese）, is well known as an effective traditional combination of herbs for treating cardiovascul...Objective： Cheongseoikki-tang （CIT, Korean）, also called Qingshu Yiqi decoction（清署益气汤）and Seisho-ekki-to （Japanese）, is well known as an effective traditional combination of herbs for treating cardiovascular diseases. This study was to research its effects on bone marrow-derived mast cell （BMMC）-mediated allergy and inflammation mechanisms. Methods： In this study, the biological effect of Cheongseoikki-tang ethanol extract （CITE） was evaluated, focusing on its effects on the production of allergic mediators by phorbol 12-myfistate 13-acetate （PMA） plus calcium ionophore A23187 （A23187）-stimulated BMMCs. These allergic mediators included intedeukin-6 （IL-6）, prostaglandin D2 （PGD2）, leukotfiene （34 （LTC4）, and β-hexosaminidase （13-hex）. Results： Our data revealed that CITE inhibited the production of IL-6, PGD2,/TC4, and 15-hex induced by PMA plus A23187 （P〈0.05）. Conclusion： These findings indicate that CITE has the potential for use in the treatment of allergy.展开更多
目的:探讨钙结合蛋白S100A4调节小鼠骨髓源肥大细胞(BMMC)活化的作用。方法:8~10周龄野生型(WT)和S 100 A 4基因敲除(S 100 A 4-/-)C57BL/6健康雄性小鼠各2只,取胫骨与股骨提取骨髓进行骨髓源肥大细胞(BMMC)培养并鉴定;以成熟WT BMMC为...目的:探讨钙结合蛋白S100A4调节小鼠骨髓源肥大细胞(BMMC)活化的作用。方法:8~10周龄野生型(WT)和S 100 A 4基因敲除(S 100 A 4-/-)C57BL/6健康雄性小鼠各2只,取胫骨与股骨提取骨髓进行骨髓源肥大细胞(BMMC)培养并鉴定;以成熟WT BMMC为模型细胞,实验分为S100A4蛋白处理组(1500μg/L S100A4蛋白)和磷酸盐缓冲液(PBS)对照组(S100A4蛋白等量体积PBS),分别于培养第1、2及3周采用甲苯胺蓝染色鉴定小鼠成熟BMMC,采用化学发光法检测各组小鼠成熟BMMC中β-氨基己糖苷酶(β-hex)的吸光度(OD),采用流式细胞术检测各组小鼠成熟BMMC的S100A4蛋白表达和白细胞介素-5(IL-5)、白细胞介素-6(IL-6)、白细胞介素-13(IL-13)及肿瘤坏死因子-α(TNF-α)的荧光强度并计算相对荧光指数(rFI);以成熟WT和S 100 A 4-/-BMMC为模型细胞,实验分为WT离子霉素(ION)处理组(1500μg/L ION)、WT PBS对照组(ION等量体积PBS)、S 100 A 4-/-ION处理组(1500μg/L ION)及S 100 A 4-/-PBS对照组(ION等量体积PBS),采用化学发光法检测各组小鼠成熟BMMC中β-hex的吸光度(OD)。结果:BMMC培养至3周时基本成熟,并表达S100A4蛋白;S100A4蛋白处理组成熟BMMC中β-hex、IL-5、IL-6、IL-13及TNF-α水平高于PBS对照组(P<0.05或P<0.01);WT ION处理组成熟BMMCβ-hex、IL-5、IL-6、IL-13及TNF-α水平高于WT PBS对照组(P<0.01),S 100 A 4-/-ION处理组成熟BMMC中β-hex、IL-5及IL-6明显高于S 100 A 4-/-PBS对照组(P<0.05),S 100 A 4-/-ION处理组成熟BMMC中β-hex、IL-5、IL-6、IL-13及TNF-α水平均低于WT ION处理组(P<0.05)。结论:S100A4蛋白能够直接诱导成熟BMMC活化,S 100 A 4基因的缺失可抑制成熟BMMC活化及炎性因子的产生。展开更多
基金Supported by the National Research Foundation of Korea Grant (No.NRF-2010-013-E00034)the Grant of the Traditional Korean Medicine R&D project,Ministry of Health & Welfare, Republic of Korea(No.B120069)
文摘Objective： Cheongseoikki-tang （CIT, Korean）, also called Qingshu Yiqi decoction（清署益气汤）and Seisho-ekki-to （Japanese）, is well known as an effective traditional combination of herbs for treating cardiovascular diseases. This study was to research its effects on bone marrow-derived mast cell （BMMC）-mediated allergy and inflammation mechanisms. Methods： In this study, the biological effect of Cheongseoikki-tang ethanol extract （CITE） was evaluated, focusing on its effects on the production of allergic mediators by phorbol 12-myfistate 13-acetate （PMA） plus calcium ionophore A23187 （A23187）-stimulated BMMCs. These allergic mediators included intedeukin-6 （IL-6）, prostaglandin D2 （PGD2）, leukotfiene （34 （LTC4）, and β-hexosaminidase （13-hex）. Results： Our data revealed that CITE inhibited the production of IL-6, PGD2,/TC4, and 15-hex induced by PMA plus A23187 （P〈0.05）. Conclusion： These findings indicate that CITE has the potential for use in the treatment of allergy.
文摘目的:探讨钙结合蛋白S100A4调节小鼠骨髓源肥大细胞(BMMC)活化的作用。方法:8~10周龄野生型(WT)和S 100 A 4基因敲除(S 100 A 4-/-)C57BL/6健康雄性小鼠各2只,取胫骨与股骨提取骨髓进行骨髓源肥大细胞(BMMC)培养并鉴定;以成熟WT BMMC为模型细胞,实验分为S100A4蛋白处理组(1500μg/L S100A4蛋白)和磷酸盐缓冲液(PBS)对照组(S100A4蛋白等量体积PBS),分别于培养第1、2及3周采用甲苯胺蓝染色鉴定小鼠成熟BMMC,采用化学发光法检测各组小鼠成熟BMMC中β-氨基己糖苷酶(β-hex)的吸光度(OD),采用流式细胞术检测各组小鼠成熟BMMC的S100A4蛋白表达和白细胞介素-5(IL-5)、白细胞介素-6(IL-6)、白细胞介素-13(IL-13)及肿瘤坏死因子-α(TNF-α)的荧光强度并计算相对荧光指数(rFI);以成熟WT和S 100 A 4-/-BMMC为模型细胞,实验分为WT离子霉素(ION)处理组(1500μg/L ION)、WT PBS对照组(ION等量体积PBS)、S 100 A 4-/-ION处理组(1500μg/L ION)及S 100 A 4-/-PBS对照组(ION等量体积PBS),采用化学发光法检测各组小鼠成熟BMMC中β-hex的吸光度(OD)。结果:BMMC培养至3周时基本成熟,并表达S100A4蛋白;S100A4蛋白处理组成熟BMMC中β-hex、IL-5、IL-6、IL-13及TNF-α水平高于PBS对照组(P<0.05或P<0.01);WT ION处理组成熟BMMCβ-hex、IL-5、IL-6、IL-13及TNF-α水平高于WT PBS对照组(P<0.01),S 100 A 4-/-ION处理组成熟BMMC中β-hex、IL-5及IL-6明显高于S 100 A 4-/-PBS对照组(P<0.05),S 100 A 4-/-ION处理组成熟BMMC中β-hex、IL-5、IL-6、IL-13及TNF-α水平均低于WT ION处理组(P<0.05)。结论:S100A4蛋白能够直接诱导成熟BMMC活化,S 100 A 4基因的缺失可抑制成熟BMMC活化及炎性因子的产生。
