A Reverse-transcription Recombinase-aided Amplification Assay for the Rapid Detection of the Far-Eastern Subtype of Tick-borne Encephalitis Virus

Objective Tick-borne encephalitis virus(TBEV) is an emerging pathogen in Europe and North Asia that causes tick-borne encephalitis(TBE). A simple, rapid method for detecting TBEV RNA is needed to control this disease. Methods A reverse-transcription recombinase-aided amplification(RT-RAA) assay was developed. This assay can be completed in one closed tube at 39℃ within 30 minutes. The sensitivity and specificity of RT-RAA were validated using non-infectious synthetic RNA representing a fragment of the NS5 region of the wild-type(WT) TBEV genome and the Senzhang strain. Additionally, 10 batches of tick samples were used to evaluate the performance of the RT-RAA assay. Results The analytical limit of detection of the assay was 20 copies per reaction of the TBEV synthetic transcript and 3 plaque-forming units(pfu) per reaction of TBEV titers. With the specific assay, no signal due to other arboviruses was observed. Of the 10 batches of tick samples obtained from the Changbai Mountains of China, three were TBEV-positive, which was consistent with the results of the quantitative real-time PCR assay. Conclusion A rapid, highly sensitive, specific, and easy-to-use method was developed for the detection of the TBEV Far-Eastern subtype.

Numerical Analysis of Stochastic Vector Borne Plant Disease Model

We are associating the solutions of stochastic and deterministic vector borne plant disease model in this manuscript.The dynamics of plant model depends upon threshold number P^(∗).If P^(∗)<1 then condition helpful to eradicate the disease in plants while P^(∗)>1 explains the persistence of disease.Inappropriately,standard numerical systems do not behave well in certain scenarios.We have been proposed a structure preserving stochastic non-standard finite difference system to analyze the behavior of model.This system is dynamical consistent,positive and bounded as defined by Mickens.

SEIR Model and Simulation for Vector Borne Diseases

An epidemic model is a simplified means of describing the transmission of infectious diseases through individuals. The modeling of infectious diseases is a tool which has been used to study the mechanisms by which diseases spread, to predict the future course of an outbreak and to evaluate strategies to control an epidemic. Epidemic models are of many types. Here, SEIR model is discussed. We first discuss the basics of SEIR model. Then it is applied for vector borne diseases. Steady state conditions are derived. A threshold parameter R0 is defined and is shown that the disease will spread only if its value exceeds 1. We have applied the basic model to one specific diseases-malaria and did the sensitivity analysis too using the data for India. We found sensitivity analysis very important as it told us the most sensitive parameter to be taken care of. This makes the work more of practical use. Numerical simulation is done for vector and host which shows the population dynamics in different compartments.

Combined iterative cross-correlation demodulation scheme for mixing space borne automatic identification system signals

Aiming at the potential presence of mixing automatic identification system(AIS) signals,a new demodulation scheme was proposed for separating other interfering signals in satellite systems.The combined iterative cross-correlation demodulation scheme,referred to as CICCD,yielded a set of single short signals based on the prior information of AIS,after the frequency,code rate and modulation index were estimated.It demodulates the corresponding short codes according to the maximum peak of cross-correlation,which is simple and easy to implement.Numerical simulations show that the bit error rate of proposed algorithm improves by about 40% compared with existing ones,and about 3 dB beyond the standard AIS receiver.In addition,the proposed demodulation scheme shows the satisfying performance and engineering value in mixing AIS environment and can also perform well in low signal-to-noise conditions.

PRELIMINARY STUDIES ON AIR-BORNE SPORES AND POLLENS IN NORTHERN SUBURB OF BEIJING

Air borne spores and pollens were collected by using Durham Pollen Sampler from 38 metre building top platform in northern suburb of Beijing and observations were carried out accordingly. Distributed mainly in March to October in a year, both the amount and variations of pollens are affected by air temperature and precipitation in addition to that of florescence. The maximum pollen species are found in May, and the maximum pollen amount is found in early June, and then in September. Pollens of herbs predominate, mostly distributing in May to September. Xylophytes have smaller amount of pollens which are found mostly in March to June. The amount of air borne plant pollen and bacterial spores is much higher in hot sunny days than in the cloudy/rainy days. The alternation of hot sunny days with cloudy/rainy days is most favourable for bacterial spores dispersion.

Finite element analysis of dynamic response and structure borne noise of gearbox

A dynamic finite element method combined with finite element mixed formula for contact problem is used to analyze the dynamic characteristics of gear system. Considering the stiffness excitation, error excitation and meshing shock excitation, the dynamic finite element model is established for the entire gear system which includes gears, shafts, bearings and gearbox housing. By the software of I-DEAS, the natural frequency, normal mode, dynamic time-domain response, frequency-domain response and one-third octave velocity grade structure borne noise of gear system are studied by the method of theoretical modal analysis and dynamic response analysis. The maximum values of vibration and structure borne noise are occurred at the mesh frequency of output grade gearing.

REALIZATION OF QUICK-LOOK IMAGING FOR SPACEBORNE SAR BASED ON PARALLEL PROCESSING

Large range cell migration is a severe challenge to imaging algorithm for spaceborne SAR. Based on design of Finite Impulse Response (FIR) filter and Range Doppler (RD) algorithm, a realization of quick-look imaging for large range cell migration is proposed. It realized quick-look imaging of 8 times reduced resolution with parallel processing on memory shared 8 CPU SGI server. According to simulation experiment, this quick-look imaging algorithm with parallel processing can image 16384x16384 SAR raw data within 6 seconds. It reaches the requirement of real-time imaging.

Spatial Risk Analysis of Water Borne Diseases (Bovine Leptospirosis) in the Rive Nile State-Sudan

Geographical Information Science (GIS) technologies have been used increasingly for ecology and epidemiology of water-borne diseases, providing approach for animal health issues. This study was set up to investigate the geographical distribution of Bovine that was affected by Leptospira hardijo, in River Nile state, on October 2012. Locations of targeted cattle were delimited using GPS. Fifty three (53) of blood samples were collected, and screened in the laboratory for Leptospira hardijo specific antibodies using indirect ELISA. 15.09% had evidence of infection as determined by the presence of anti-leptospira antibodies. It was inferred that no incidences were recorded in 45 locations out of the 53 selected locations in the state. Leptospirosis risk area for transmission was mapped using 5 km buffer distance. Animals’ movements routes were mapped with their contacts area and positive samples locations, hafair locations where animals contacts were mapped. This study demonstrated the value of GIS and GPS in disease mapping for animals’ health, and this might help veterinary authorities to implement strategic interventions for animal disease control.

Effect of Soil Amendments on Soil Borne Pathogens of French Bean （Phaseolus vulgaris L,） in Organic Farming System in Manipur

The rationale behind the use of soil amendments by traditional marginal farmers against soil borne pathogen is an area of interest. The present work ascertain the effect of certain soil amendments against pre emergent and post emergent rot of French bean in the agro climatic condition of Manipur, Four soil amendments： cow dung （COD）, sawdust （SD） ＋ paddy husk （PH）, decomposed floating phytomat （DFP） was tried in field conditions in four different combinations. These have been often used by the traditional farmers. A locally available commonly cultivated pole type of French bean has been used for the study. Disease incidences in plots treated with these amendments were then compared with that of control plots where no amendments were made. The plots with a combined treatment of the soil amendments showed maximum seed germination and least post emergent rot against sclerotinia rot. But DFP showed effectiveness against rhizoctonia rot with maximum seed germination while saw dust showed maximum decrease in post emergent rot against rhizoctonia rot. The plots treated with organic soil amendments showed statistically significant positive effect against the pathogens as compared with untreated control plots.

Knowledge Attitude and Practices Related on Prevention of Mosquito Borne Diseases and Sanitation Conditions in a Salvadoran Urban Community

Introduction: In El Salvador Mosquito Borne Diseases (MBDs) is a serious public health problem due to produce morbidity and mortality. They produce a high economic loss, which contributes to the collapse of the public health system. Over the years, the Salvadoran public health system has invested large amounts of resources to minimize the problem through campaigns against MBDs. Despite this, the population is still affected by diseases such as dengue, and more recently chikungunya and zika outbreaks. The aim of this study is to evaluate sanitation conditions variables and identify knowledge, attitudes, practices related to the prevention of MBDs. Methods: A cross-sectional survey about sanitation conditions and knowledge, attitudes and practices on prevention of MBDs of inhabitant’s ≥ 15 years from an urban community of a municipality with the highest number of cases reported in 2015. Results: Majority of respondents (98.2%) had direct supply of drinking water;however 96.4% of respondents were agreed that they had an inconsistent water supply. Regarding MBDs prevention respondents showed high acceptable knowledge (76.8%), high favorable attitude (92.1%) and an acceptable implementation of practices toward prevention reproduction of mosquitoes (58.5%) and a poor implementation of practices to prevent mosquito bites (38.3%). Conclusions: The findings revealed high acceptable knowledge about MBDs and a high favorable attitude regarding to prevent them, but also revealed a scarce implementation of prevention practices. The generalized storage of water on non-covered barrels because the inconsistent water supplies, can be source of proliferation of mosquitoes and therefore increases the risk of transmission of MBDs. High population density of the community can magnify this risk. It is necessary to enhance education to promote better implementation of practices using the most common media together with the local health workers.

Seed Borne Fungal Organisms Associated with Germination Success of Terminalia brownii (Fresen) in Kenya

Terminalia brownii is an indigenous tree species highly valued in the Kenyan drylands for its products such as timber for wood curving, medicine, and charcoal production, among others. Due to this high value followed by overutilization, its population in the drylands is dwindling. Concern about the species’ low regeneration as shown by low germination has been raised. This research was conducted on the seed borne fungal organisms that are associating with the germination of this species. T. brownii seeds were collected from cluster patches of the species in Kendu Bay, Kimose and Ndumoni in Homabay, Baringo and Kitui counties of Kenya respectively. The seeds were harvested for fruit probing for maturity confirmation. Random sampling was used in selecting thirty trees, 50 meters apart, from which fresh fruits were uniformly collected from the crowns. The research adopted a complete block experimental design where one hundred seeds each from the three sites, were subjected to a germination test, seed borne fungal organisms’ presence, identified organisms cultured and DNA and DNA sequencing carried out for identification. Fungal organisms associated with germination success of T. brownii were Fusarium equiseti, Pestalotia sp, and Alternaria alternata. Paired t test run between germinants versus the number of sown seeds gave N = 12, p-value of 0.000, t = 16.29. On the other hand, paired t test run between fungal infected seeds with the number of germinants gave N = 12, p-0.000, t = 8.78. Fungi associated with germinants included Penicillium sp, and the data analyzed using Mann-Whitney U test run showed significant difference at a p value of 0.000. Identified organisms associating with none germination and germination success on the other hand were analyzed using descriptive analysis. Fungal organisms associating with germination success of T. brownii significantly affect germination of freshly extracted seeds from the field, hence, for maximum germination achievement, seeds should be sown while still fresh.

Tick-borne Diseases,Transmission,Host Immune Responses,Diagnosis and Control

Present review article explains tick-borne diseases,transmission,host immune responses,diagnosis and control in relation to climatic variations.Ticks are hematophagous ectoparasites which suck large volumes of blood from livestock and humans.They release large numbers of protozoans,bacteria,rickettsia and viral pathogens during blood feeding and transmit disease pathogens through saliva.Due to heavy blood sucking by ticks animals face significant blood and weight loss that affect their overall health.Due to more severe illness,high economic losses were noted in livestock.This article highlights medically important tick borne diseases in man and livestock,its pathogenesis,diagnosis and treatment methods.The present article emphasizes invasion of hosts,host-pathogen interactions,tick saliva toxin induced host immune responses and biological effects.This article highlighted various tick control methods i.e.physical killing,acaricidal,biological,hormonal,genetic and immunological methods such as administration of protective antibody and vaccines for disease control in human being and his livestock.The authors suggest non-chemical environmentally safe methods for successful control of tick borne diseases to kill cattle,bird and canine invading ticks.

Pathogenic Surveillance of Foodborne Illness-Related Diarrhea—Beijing Municipality,China,2013-2023

What is already known about this topic?Foodborne diseases present a significant public health concern,particularly in China,where they represent a significant food safety challenge.Currently,there is a need for a thorough and systematic analysis of the extended epidemiological patterns of foodborne diseases in Beijing Municipality.What is added by this report?Monitoring results show that Norovirus and diarrheagenic Escherichia coli(DEC)are the most commonly identified foodborne diarrheal pathogens.Individuals aged 19–30 are at a higher risk of foodborne diarrhea in Beijing,with Salmonella infection being associated with fever symptoms.What are the implications for public health practice?This study analyzes 11 years of consecutive monitoring data to enhance understanding of the epidemiological and clinical features of foodborne diarrhea in Beijing.It aims to identify high-risk populations,assist in clinical pathogen identification and treatment,and support the development of tailored preventive strategies.

Vector-borne disease and climate change adaptation in African dryland socialecological systems

Background:Drylands,which are among the biosphere's most naturally limiting and environmentally variable ecosystems,constitute three-quarters of the African continent.As a result,environmental sustainability and human development along with vector-borne disease(VBD)control historically have been especially challenging in Africa,particularly in the sub-Saharan and Sahelian drylands.Here,the VBD burden,food insecurity,environmental degradation,and social vulnerability are particularly severe.Changing climate can exacerbate the legion of environmental health threats in Africa,the social dimensions of which are now part of the international development agenda.Accordingly,the need to better understand the dynamics and complex coupling of populations and environments as exemplified by drylands is increasingly recognized as critical to the design of more sustainable interventions.Main body:This scoping review examines the challenge of vector-borne disease control in drylands with a focus on Africa,and the dramatic,ongoing environmental and social changes taking place.Dryland societies persisted and even flourished in the past despite changing climates,extreme and unpredictable weather,and marginal conditions for agriculture.Yet intrusive forces largely out of the control of traditional dryland societies,along with the negative impacts of globalization,have contributed to the erosion of dryland's cultural and natural resources.This has led to the loss of resilience underlying the adaptive capacity formerly widely exhibited among dryland societies.A growing body of evidence from studies of environmental and natural resource management demonstrates how,in light of dryland system's inherent complexity,these factors and top-down interventions can impede sustainable development and vector-borne disease control.Strengthening adaptive capacity through community-based,participatory methods that build on local knowledge and are tailored to local ecological conditions,hold the best promise of reversing current trends.Conclusions:A significant opportunity exists to simultaneously address the increasing threat of vector-borne diseases and climate change through methods aimed at strengthening adaptive capacity.The integrative framework and methods based on social-ecological systems and resilience theory offers a novel set of tools that allow multiple threats and sources of vulnerability to be addressed in combination.Integration of recent advances in vector borne disease ecology and wider deployment of these tools could help reverse the negative social and environmental trends currently seen in African drylands.

Boron-doped high-entropy oxide toward high-rate and long-cycle layered cathodes for wide-temperature sodium-ion batteries

03-type layered metal oxides hold great promise for sodium-ion batteries cathodes owing to their energy density advantage.However,the severe irreversible phase transition and sluggish Na^(+)diffusion kinetics pose significant challenges to achieve high-performance layered cathodes.Herein,a boron-doped03-type high entropy oxide Na(Fe_(0.2)Co_(0.15)Cu_(0.05)Ni_(0.2)Mn_(0.2)Ti_(0.2))B_(0.02)O_(2)(NFCCNMT-B_(0.02))is designed and the covalent B-O bonds with high entropy configuration ensure a robust layered structure.The obtained cathode NFCCNMT-B_(0.02)exhibits impressive cycling performance(capacity retention of 95%and 82%after100 cycles and 300 cycles at 1 and 10 C,respectively)and outstanding rate capability(capacity of 83 mAh g^(-1)at 10 C).Furthermore,the NFCCNMT-B_(0.02)demonstrates a superior wide-temperature performance,maintaining the same capacity level(113,4 mAh g^(-1)@-20℃,121 mAh g^(-1)@25℃,and 119 mAh g^(-1)@60℃)and superior cycle stability(90%capacity retention after 100 cycles at 1 C at-20℃).The high-entropy configuration design with boron doping strategy contributes to the excellent sodium-ion storage performance.The high-entropy configuration design effectively suppresses irreversible phase transitions accompanied by small volume changes(ΔV=0.65 A3).B ions doping expands the Na layer distance and enlarges the P3 phase region,thereby enhancing Na^(+)diffusion kinetics.This work offers valuable insights into design of high-performance layered cathodes for sodium-ion batteries operating across a wide temperature.

Some Notable Chinese in Dublin in the Mid-Twentieth Century

The careers of three Chinese physicists,Hu Ning,Ma Shijun,and Peng Huanwu at the Dublin Institute for Advance Studies in the 1940s,and later,are described.A brief history of the foundation and operations of the institute,as well as the roles in it of Erwin Schrodinger,Walter Heitler,Max Born,and others are included.Some details are given of the works carried out there.The three men's post-institute careers are described,Ma eventually in Australia,and Hu and Peng in the People's Republic of China where they became distinguished leaders of theoretical physics research.

障碍干扰下的压缩感知电磁成像算法

基于电磁成像模型,针对逆散射问题的病态性和非线性性质,引入压缩感知(CS)中的全变分(TV)算法,旨在减少所需天线数量,并提高电磁成像的图像质量.在玻恩(Born)迭代的基础上,引入全变分压缩感知算法(TV-CS).仿真结果显示:即使目标被障碍物遮挡,该算法也能够在配置较少探测天线的情况下,对目标位置和形状进行准确的重构.

Powassan virus:A tick borne flavivirus infecting humans

Infection of humans by Powassan virus(POWV)occurs rarely but is potentially life‐threatening.First isolated in Ontario,Canada in 1958,the presence of POWV has been confirmed in three countries:Canada,the USA,and Russia.Although a limited number of human cases has been reported thus far,the infection rate has shown signs of increasing during the 21^(st) century.Interestingly,POWV and a genetically close variant,deer tick virus(DTV),are the only member of the tick‐borne flaviviruses known to be endemic in North America and maintain in respective tick‐host cycles.In this review,we briefly summarize current knowledge involving the epidemiology and etiology,pathogenesis and immunity,molecular evolution,and protein functions of POWV,aiming to increase our understanding of the virus and unlock the potential to control this lethal pathogen.These data may also provide tools to minimize the future threat of other emerging and re‐emerging viruses.

Role of stanniocalcin1 in brain injury of coal-burning-borne fluorosis rats

Objective To observe the change of stanniocalcin 1(STC1) and calcium content in brain of coal-burning-borne fluorosis rats,and to explore the role of STC1 in brain injury of coal-burning-borne fluorosis.Methods Twenty four male SD rats were randomly divided into control,low,medium,