Molecular Identification of Mycobacterium Strains Responsible of Bovine Tuberculosis Cases in Bobo-Dioulasso Slaughterhouse, Burkina Faso

Bovine tuberculosis (bTB) is an endemic zoonosis significantly affects animal health in Burkina Faso. The primary causative agent is Mycobacterium tuberculosis (M. tuberculosis) complex, mainly M. bovis. Cattle are considered as natural reservoir of M. bovis. However, in Burkina Faso, the circulation of these strains remains poorly understood and documented. This study aimed to identify and characterize Mycobacterium strains from suspected carcasses during routine meat inspection at Bobo-Dioulasso refrigerated slaughterhouse. A prospective cross-sectional study was conducted from January 2021 to December 2022 on cases of seizures linked to suspected bovine tuberculosis. Microbiological and molecular analyzes were used for mycobacterial strain isolation and characterization. Out of 50 samples, 24% tested positive by microscopy and 12% by culture. Molecular analysis identified 6 strains of Mycobacteria, exclusively Mycobacterium bovis specifically the subspecies bovis (Mycobacterium bovis subsp bovis). In conclusion, M. bovis subsp bovis is the primary agent responsible for bovine tuberculosis in Bobo-Dioulasso. Continuous monitoring of mycobacterial strains is therefore necessary for the effective control of this pathology in the local cattle population.

Evaluation and application of a milk antibody ELISA for assessing the prevalence and incidence of bovine tuberculosis in dairy herds in Hubei Province,China

Bovine tuberculosis(bTB)is a chronic zoonotic disease that is endemic in China.Current in-vitro tests for bTB are mainly based on blood assays.Collection of samples results in some stress to the sampled cattle and associated economic losses for the herd owner.This study was designed to investigate the relationship between milk and serum antibody tests for bTB in dairy cows using 85 cows with milk and corresponding blood samples.Totally 4,395 milk samples were used to assesse the apparent(test)prevalence and incidence of bTB using the milk antibody ELISA.The association between levels of bTB milk antibody and milk quality was also evaluated.Milk and serum antibody tests showed a good correlation with a 87.5%(95%CI:61.7%,98.4)positive agreement and 98.7%(95%CI:95.4,99.8)negative agreement.The animal level lactoprevalence ranged from 0.3%(95%CI:0,1.2)to 33.3%(95%CI:26.6,40.6)in different farms and the incidence rate ranged from 0 head/cow-month(95%CI:0,0.02)to 0.04 head/cow-month(95%CI:0.02,0.07).Twenty percent of sampled farms met the criteria for bTB control in China.The prevalence on large-scale farms was lower(p<0.001)than on small farms.The bTB milk antibody levels had a negative correlation with milk yield and a positive correlation with somatic cell count(SCC),milk protein percentage(MPP)and percentage of total solids(TS).According to this research,milk ELISA could be used as a supplement of blood samples to assist in the surveillance for bTB and for alerting control and eradication of bTB.

Investigating Prevalence of Bovine Tuberculosis in Cattle in an Agro-Pastoral Community in Awdal Region, Somaliland

Awdal region is the most northwesterly province of Somaliland. The region is one of the agro-pastoral livelihood zones in Somaliland, where farming and agricultural production are the predominant livelihood sources. IGAD (Intergovernmental Authority on Development) Sheikh Technical University of Science (ISTUS) worked to study the prevalence of Bovine Tuberculosis (TB) among cattle in Awdal region of Somaliland. The aim was to inform public health and Veterinary experts on having one-health approach to infectious diseases among humans and animals. The serum that was stored at -20?C was transported to IGAD Sheikh Technical University of Science (ISTUS) for further analysis using BOVIGAM serological test (Sandwich ELISA). The results indicate that Bovine Tuberculosis is highly prevalent in the study area (10.1%). The high prevalence recorded in the current study could be due to the consumption of raw milk and lack of proper control strategies in place to control the transmission of the disease between animals, between animals and wildlife and between animals and humans. Hence, an awareness creation campaign should be created on bTB transmission and its public health significance to cattle owners, milk and meat consumers and people who are in close proximity to cattle. In addition, testing and eradication programme should be implemented where applicable.

A review of tuberculosis at the wildlife-livestock-human interface in Zambia

Zambia’s estimated incidence of all forms of human tuberculosis(TB)is 707/100,000.High prevalence of bovine tuberculosis(BTB)-infection with Mycobacterium bovis-in cattle and the Kafue lechwe antelopes(Kobus leche Kafuensis)has been reported in the Kafue basin.Consumption of unpasteurised milk and meat products from infected animals poses a risk of transmitting zoonotic tuberculosis to people living at the human-animal interface.Despite the reported high prevalence of BTB in both livestock and wildlife,information on the proportion of human patients infected with M.bovis is unknown in Zambia.This paper reviews the available information in English on human,livestock and wildlife TB in Zambia with the purpose of assessing the burden of animal infections with M.tuberculosis complex and its public health implications.

牛结核病检测方法研究进展

牛结核病(bovine tuberculosis, bTB)是一种主要由牛分枝杆菌引起的人兽共患病。各国通常采用"检疫-扑杀"的措施来降低牛结核病造成的经济损失并以此来保障公共卫生安全。文章综述了基于病原体水平、免疫蛋白(及其衍生物)水平和核酸(分子)水平的牛结核病检测方法的部分研究成果,以期为牛结核病的检疫与临床诊断提供理论依据。